LOCUS       RR212026                9033 bp    DNA     circular  SYN 
DEFINITION  Insr (Myc-DDK-tagged ORF) - Rat insulin receptor (Insr), (10 ug)
ACCESSION   NM_017071
VERSION     NM_017071.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5190..5281 
                     /label="Myc-DDK"  
     misc_feature    5311..5896 
                     /label="PolyA signal"  
     misc_feature    5368..5387 
                     /label="Restriction Enzyme"  
     misc_feature    6172..6844 
                     /label="ColE1"  
     misc_feature    7274..8068 
                     /label="Kan/Neo"  
     misc_feature    8103..8405 
                     /label="SV40 ori"  
     misc_feature    8491..8968 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5172..5178 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5172 
                     /label="Insr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTCCGGG AGAGGATGTG AGACGACGGC GGTGCCGTTG CTGATGGCCG 
     1081 TGGCCGCGTT GCTGGTGGGC ACAGCCGGCC ACCTGTACCC TGGAGAGGTG TGCCCGGGAA 
     1141 TGGACATCCG GAACAACCTG ACCAGGTTGC ATGAGCTGGA GAACTGCTCG GTCATTGAGG 
     1201 GCCATCTGCA GATCCTCTTG ATGTTCAAGA CCAGACCCGA AGATTTCCGA GACCTCAGTT 
     1261 TCCCCAAACT CATCATGATC ACAGACTACC TGCTTCTTTT CCGTGTCTAT GGCCTAGAAA 
     1321 GTCTAAAAGA CCTCTTCCCA AACCTCACAG TCATCCGAGG CTCCCGTCTC TTCTTCAACT 
     1381 ATGCCCTGGT CATCTTCGAG ATGGTCCACC TGAAGGAGCT GGGTCTTTAC AACCTCATGA 
     1441 ACATCACCCG GGGCTCTGTC CGCATCGAGA AGAATAATGA ACTCTGCTAC CTGGCCACTA 
     1501 TCGACTGGTC CCGCATCCTG GATTCCGTGG AGGACAACTA CATTGTACTG AACAAAGATG 
     1561 ACAATGAGGA ATGTGGGGAC GTCTGTCCAG GCACAGCCAA GGGCAAGACC AACTGCCCTG 
     1621 CCACCGTCAT CAATGGGCAG TTTGTGGAAC GGTGCTGGAC ACACAGTCAT TGTCAGAAAG 
     1681 TTTGCCCAAC CATCTGTAAG TCACATGGCT GCACAGCTGA AGGCCTGTGC TGTCACAAGG 
     1741 AGTGCCTGGG CAACTGTTCA GAACCCGATG ACCCTACCAA GTGTGTGGCC TGTCGAAACT 
     1801 TCTACCTGGA TGGTCAGTGT GTGGAGACCT GCCCACCACC CTACTATCAC TTCCAGGATT 
     1861 GGCGCTGTGT AAACTTCAGC TTCTGCCAAG ACCTTCACTA CAAATGCAGG AACTCTCGGA 
     1921 AGCCTGGCTG TCACCAATAC GTCATTCACA ACAACAAGTG CATCCCCGAG TGCCCGTCTG 
     1981 GCTATACCAT GAATTCCAGC AACTTGATGT GCACCCCGTG TCTGGGACCC TGTCCTAAGG 
     2041 TCTGCCAAAT CCTCGAAGGC GAGAAGACCA TTGATTCCGT GACATCTGCC CAGGAGCTCC 
     2101 GAGGCTGCAC TGTGATCAAT GGCAGCCTGA TTATCAACAT CCGAGGGGGC AACAACCTGG 
     2161 CAGCTGAACT AGAGGCTAAC CTTGGCCTCA TTGAAGAAAT CTCAGGGTTC CTAAAGATCC 
     2221 GTCGCTCCTA TGCTCTGGTG TCACTTTCTT TCTTCAGGAA GCTACATCTG ATTCGAGGAG 
     2281 AGACCTTGGA AATTGGGAAC TATTCTTTCT ACGCCTTGGA CAACCAGAAC CTGAGGCAGC 
     2341 TGTGGGACTG GAACAAGCAC AACCTCACGA TCACTCAGGG CAAGCTCTTC TTCCACTACA 
     2401 ACCCCAAACT CTGCCTGTCT GAGATCCACA AGATGGAGGA AGTGTCCGGA ACCAAGGGCC 
     2461 GCCAGGAGAG GAACGACATT GCCCTGAAGA CCAACGGGGA CCAGGCATCC TGTGAAAATG 
     2521 AATTACTTAA ATTCTCTTTC ATCCGGACGT CTTTTGACAA GATCTTGCTG AGGTGGGAGC 
     2581 CCTACTGGCC CCCCGACTTC CGAGATCTCC TGGGATTCAT GCTGTTCTAC AAAGAGGCCC 
     2641 CTTATCAGAA CGTGACAGAG TTTGACGGGC AGGATGCGTG TGGCTCCAAC AGCTGGACTG 
     2701 TGGTGGATAT TGACCCGCCC CAGAGGTCCA ACGACCCTAA GTCTCAGACC CCAAGCCACC 
     2761 CTGGGTGGCT GATGCGGGGC CTCAAACCCT GGACCCAGTA TGCCATCTTT GTCAAGACCT 
     2821 TGGTTACCTT CTCTGATGAA CGCCGGACCT ATGGAGCCAA AAGTGATATC ATCTATGTGC 
     2881 AAACAGATGC CACCAATCCT TCCGTTCCCC TGGATCCCAT ATCAGTTTCC AACTCCTCAT 
     2941 CTCAGATTAT CTTAAAGTGG AAGCCCCCCT CGGACCCCAA TGGCAACATC ACACACTACC 
     3001 TGGTCTACTG GGAGAGGCAG GCGGAGGACA GCGAGCTGTT CGAGTTGGAT TATTGTCTCA 
     3061 AAGGGCTGAA GCTCCCCTCA CGCACCTGGT CCCCACCTTT TGAGTCTGAC GATTCTCAAA 
     3121 AGCACAATCA GAGTGAGTAT GACGACTCGG CCAGCGAGTG CTGCTCATGT CCTAAGACTG 
     3181 ACTCTCAGAT CCTGAAGGAG CTGGAGGAGT CTTCATTCAG GAAGACCTTC GAGGATTACC 
     3241 TGCACAACGT GGTTTTTGTT CCCAGAAAAA CCTCTTCAGG CAATGGTGCT GAGGACACTA 
     3301 GGCCATCCCG AAAGCGAAGA TCCCTTGAAG AGGTGGGCAA TGTGACAGCC ACTACACCCA 
     3361 CACTTCCAGA TTTTCCCAAC ATCTCCTCCA CCATCGCGCC CACAAGCCAC GAAGAGCACA 
     3421 GACCATTTGA GAAAGTAGTA AACAAGGAGT CACTTGTCAT CTCTGGCCTG AGACACTTCA 
     3481 CTGGGTACCG CATTGAGCTG CAGGCATGCA ATCAGGACTC CCCAGAAGAG AGGTGCAGCG 
     3541 TGGCTGCCTA CGTCAGTGCC CGGACCATGC CTGAAGCTAA GGCAGATGAC ATCGTTGGCC 
     3601 CTGTGACCCA TGAAATCTTT GAGAACAATG TTGTACACTT AATGTGGCAA GAGCCAAAGG 
     3661 AACCTAATGG TCTGATTGTG CTATATGAAG TGAGCTATCG GCGATATGGT GATGAGGAGC 
     3721 TGCACCTCTG TGTCTCCCGG AAGCATTTTG CCCTGGAGCG GGGCTGCAGG CTTCGAGGGC 
     3781 TCTCTCCAGG AAACTACAGT GTTCGAGTCC GGGCTACCTC TCTGGCAGGA AATGGCTCCT 
     3841 GGACAGAACC CACCTATTTT TATGTGACTG ATTATTTAGA TGTCCCATCA AATATTGCCA 
     3901 AAATTATCAT CGGGCCCCTC ATCTTCGTCT TCCTCTTCAG TGTCGTGATC GGAAGTATTT 
     3961 ATCTATTCTT GAGGAAGAGG CAGCCAGATG GGCCAATGGG ACCACTGTAC GCTTCTTCAA 
     4021 ACCCAGAGTA CCTCAGTGCC AGTGATGTCT TTCCATCTTC CGTATACGTT CCGGATGAGT 
     4081 GGGAGGTACC TCGAGAGAAG ATCACCCTCC TCCGAGAGCT GGGGCAGGGA TCCTTCGGTA 
     4141 TGGTGTACGA AGGCAATGCC AAGGATATCA TCAAGGGTGA GGTAGAGACC CGTGTTGCGG 
     4201 TGAAGACGGT CAATGAGTCA GCCAGTCTTC GAGAACGGAT CGAGTTCCTC AATGAGGCAT 
     4261 CAGTCATGAA GGGCTTCACC TGTCATCACG TGGTCCGCCT TCTTGGGGTG GTGTCCAAAG 
     4321 GCCAGCCCAC ATTGGTAGTG ATGGAACTGA TGGCTCATGG AGACCTGAAA AGTCACCTCC 
     4381 GTTCTCTGCG GCCCGATGCT GAGAACAACC CAGGCCGTCC TCCCCCTACC TTGCAAGAAA 
     4441 TGATTCAGAT GACAGCAGAA ATTGCCGATG GCATGGCATA CTTGAACGCC AAGAAGTTTG 
     4501 TGCACCGGGA CCTGGCAGCT CGGAACTGCA TGGTTGCCCA TGATTTTACT GTCAAAATCG 
     4561 GAGACTTTGG AATGACGAGA GACATCTACG AGACAGATTA CTATCGGAAA GGGGGCAAGG 
     4621 GGTTGCTGCC CGTGAGGTGG ATGTCACCCG AGTCCCTGAA GGACGGAGTC TTCACTGCTT 
     4681 CTTCCGACAT GTGGTCCTTT GGGGTGGTCC TTTGGGAAAT CACCAGCCTG GCTGAGCAAC 
     4741 CTTACCAAGG CCTGTCTAAT GAACAGGTGT TGAAATTTGT CATGGATGGA GGCTATCTGG 
     4801 ATCCCCCTGA TAACTGTCCA GAGAGACTCA CTGACCTGAT GCGCATGTGC TGGCAGTTCA 
     4861 ACCCCAAGAT GAGGCCGACC TTCCTGGAAA TCGTCAACCT GCTCAAGGAC GACCTCCACC 
     4921 CCAGCTTTCC GGAAGTTTCC TTCTTCTACA GCGAGGAGAA CAAGGCTCCC GAGAGTGAAG 
     4981 AGCTGGAGAT GGAGTTCGAG GACATGGAGA ATGTCCCCTT GGATCGTTCC TCTCACTGTC 
     5041 AGAGAGAAGA GGCTGGATGC CGGGAGGGAG GGTCCTCTCT GAGCATCAAA CGGACCTATG 
     5101 ATGAACACAT CCCCTACACC CACATGAACG GGGGCAAGAA GAATGGGCGG GTCCTCACCC 
     5161 TGCCGAGGTC GAACCCTTCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5221 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5281 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5341 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5401 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5461 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5521 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5581 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5641 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5701 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5761 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5821 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5881 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5941 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6001 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6061 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6121 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6181 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6241 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6301 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6361 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6421 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6481 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6541 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6601 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6661 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6721 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6781 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6841 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6901 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6961 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7021 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7081 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7141 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7201 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7261 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7321 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7381 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7441 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7501 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7561 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7621 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7681 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7741 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7801 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7861 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7921 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7981 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8041 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8101 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8161 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8221 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8281 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8341 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8401 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8461 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8521 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8581 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8641 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8701 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8761 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8821 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8881 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8941 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9001 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//