LOCUS       RR212018                6822 bp    DNA     circular  SYN 
DEFINITION  Kif12 (Myc-DDK-tagged ORF) - Rat kinesin family member 12 (Kif12), (10 ug)
ACCESSION   NM_001012102
VERSION     NM_001012102.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2979..3070 
                     /label="Myc-DDK"  
     misc_feature    3100..3685 
                     /label="PolyA signal"  
     misc_feature    3157..3176 
                     /label="Restriction Enzyme"  
     misc_feature    3961..4633 
                     /label="ColE1"  
     misc_feature    5063..5857 
                     /label="Kan/Neo"  
     misc_feature    5892..6194 
                     /label="SV40 ori"  
     misc_feature    6280..6757 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2961..2967 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2961 
                     /label="Kif12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGAACGT GGGTCTCCCG ATGGGGACCC TGCACGGAAC CTGGAGCAAG 
     1081 GGCCAGAAGG ATCAGAAACC CCTATTCAAG TGGTGCTCAG GGTGCGTCCC ATGAGCACAG 
     1141 TGGAGCTGCG TAGAGGGGAG CAGAGTGCAC TGCACTGCTC GGGGACCCGG ACGCTGCAGG 
     1201 TGAGCCCTCC GGGTGGGGGC CCCGACGTGG CGTTCCGCTT CGGTGCCGTA CTGGACGGGG 
     1261 CGCGCACACA GGAGGACGTG TTCAGAGCTT GCGGTGTGAA GCGCCTGGGC GAGCTGGCGC 
     1321 TGCGTGGTTT CTCCTGCACC GTCTTCACTT TTGGCCAGAC TGGTTCCGGG AAAACCTACA 
     1381 CTCTGACTGG ACCTCCTCCT CAGGCAGAAG GGGTGCCCGT GCCCCCCAGC CTTGCTGGCA 
     1441 TTATGCAGAG GACCTTCACC TGGCTATTAG ATCGCATGCA GCACCTGGAT TCTCCAGTCA 
     1501 CCCTCCGAGC CTCCTACCTG GAGATCTACA ATGAGCAGGT TCAGGACTTG CTGAGTCAGG 
     1561 GGTCTCCCCG GCCTCTGCCT GTTAGGTGGT CCAAGGCACG AGGCTTCTAT GTGGAACAGC 
     1621 TTCGGTTGGT GGAGTGTGGC AGTCTGGAAG CTTTGATGGA ACTATTGCAG ATGGGTCTCA 
     1681 GCCGTCGCCG GAGCTCCTCG CATACCTTGA ACCAGGCCTC CAGCAGAAGC CATGCCCTGC 
     1741 TCACACTCCA CATCAGCCGC CCAACTTCTC AGCAGGTACC TCCTGTGGAC CTTGGGGAGC 
     1801 CCCCTGTTGG AGGAAAGCTG TGCTTCGTGG ACCTTGCAGG CAGTGAGAAG GTGGCAGCCA 
     1861 CGGGGTCCAG AGGGCAGCTG ATGCTTGAGG CCAATAGCAT TAACCGCAGC CTATTGGCAC 
     1921 TGGGCCACTG CATCTCCCTG CTGTTGGACC CACAGAGGAA GCAGAGCCAC ATCCCCTTCC 
     1981 GGGACAGCAA GCTCACCAAA TTGCTGGCAG ACTCACTTGG GGGCCGTGGG GTCACGCTCA 
     2041 TGGTTGCCTG CGTATCCCCT TCAGCACAGT GCCTTCCCGA GACCCTCAGC ACTCTGCGAT 
     2101 ACGCAAGCCG AGCTCAGCGG ATCACCACCC GGCCACAGGG TCCCAAGTCC CCTGCAGTAA 
     2161 AGCCACCCCA GCAAGTAGAG GCCGAGCTGC TACAGCTCCA AGAGGAGAAT CGTTGCCTGC 
     2221 GGCTCCAGTT GGATCAAATG TACACTAAGG CACCGAGAGT CCACGGAGCC CGGGTGGCCT 
     2281 GGGCCCAGCG GAACCTCTAT GGGATGCTGC AGGAGTTCAT GCTGGAGAAT GAGAAGCTCA 
     2341 GGAAGGAAAT GAGACAGCTT CGGAGTAGCC GGGACCTGGC CCAGGCAGAG CAGCATGTCC 
     2401 TGGCCCAGCA GATCCATGAA CTGGAGAGGC GCCTCCTTTT GGCTTGCTCC CTTCCCCAGC 
     2461 AGACCTCTGC CACCGTGTGC CCCTGCAGGA TGACTCCAGC TGCCTCCTGC CATGTACTGC 
     2521 CACCCCTCTG CTACTGCTAT CACCTCTGTC CTCTGTGCCG AGTGTCCTTG ACCCACTGGG 
     2581 CCTGCCCATG GAGAGAATGC CATGCACCAC AGGTGCTGGA GCCTGAGGCC CCAGGCCACA 
     2641 TATCTCCATC TGTGCGGCCC CCACCCTGGG CACCTCCAAC AAGCCCAGGC TCTGCCAAGC 
     2701 CCCCACAAGA GAGGAACCAT AGTGACTGGA CACAGACACG AGTCCTGGCA GAGATGCTGA 
     2761 TGGGAGAGGA AGTGGTACCC TCTGCACCAC CCCTGCCTGC AGGGCCCTCA AACATGCCAC 
     2821 AAGTGCTAAG AGGAGGATCT GGGATCCCAA ACCAGACCCC AAGACTGGAA ACCCTCACAC 
     2881 ACCAGATCAA CAGCTCCCTG AACCCCAGCC AGAGACAACC ACAGCCCAGC GAGGACACAC 
     2941 AGAGCCCTGG CCAAGGCCTC TCTTCTTATA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3001 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3061 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3121 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3181 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3241 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3301 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3361 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3421 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3481 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3541 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3601 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3661 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3721 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3781 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     3841 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     3901 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     3961 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4021 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4081 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4141 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4201 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4261 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4321 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4381 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4441 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4501 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4561 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4621 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4681 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4741 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4801 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     4861 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     4921 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     4981 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5041 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5101 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5161 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5221 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5281 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5341 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5401 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5461 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5521 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5581 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5641 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5701 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5761 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     5821 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     5881 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     5941 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6001 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6061 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6121 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6181 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6241 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6301 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6361 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6421 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6481 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6541 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6601 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6661 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6721 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6781 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//