LOCUS       RR212013                8523 bp    DNA     circular  SYN 
DEFINITION  Ttbk2 (Myc-DDK-tagged ORF) - Rat tau tubulin kinase 2 (Ttbk2), (10 ug)
ACCESSION   NM_001107766
VERSION     NM_001107766.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4678..4769 
                     /label="Myc-DDK"  
     misc_feature    4799..5384 
                     /label="PolyA signal"  
     misc_feature    4856..4875 
                     /label="Restriction Enzyme"  
     misc_feature    5660..6332 
                     /label="ColE1"  
     misc_feature    6762..7556 
                     /label="Kan/Neo"  
     misc_feature    7591..7893 
                     /label="SV40 ori"  
     misc_feature    7979..8456 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4653..4660 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4653 
                     /label="Ttbk2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGGAGGA GGAGAGCAGC CAGATATCCT CAGTGTTGGA ATCCTGGTCA 
     1081 AAGAAAGATG GAAAGTGTTA AGAAAGATTG GGGGTGGGGG CTTTGGAGAA ATTTACGATG 
     1141 CCTTGGACAT GCTCACCAGG GAGAATGTGG CACTGAAGGT GGAGTCAGCT CAGCAGCCAA 
     1201 AGCAGGTTCT GAAGATGGAG GTTGCTGTGT TGAAGAAACT GCAAGGGAAA GACCATGTTT 
     1261 GTAGATTTAT TGGTTGTGGG AGAAATGACC GTTTCAACTA CGTGGTCATG CAATTGCAGG 
     1321 GTCGGAATCT GGCAGATCTT CGACGTAGCC AATCTCGGGG CACATTCACT ATTAGCACTA 
     1381 CTCTTCGTCT CGGGAAACAG ATTCTGGAGT CTATTGAAAG CATACATTCT GTGGGATTCC 
     1441 TTCACAGAGA CATCAAACCG TCAAATTTCG CCATGGGACG CTTTCCCAGT ACATGTAGAA 
     1501 AATGTTTTAT GCTTGATTTT GGCTTGGCCC GACAATTTAC CAATTCCTGT GGTGACGTCA 
     1561 GACCACCTCG TGCTGTGGCA GGCTTCCGAG GGACAGTTCG CTATGCATCC ATCAATGCTC 
     1621 ATCGGAACAG GGAGCAAGTA GGTTCCATTA AGGAGAGGTA TGACCATAGG CTCATGTTGA 
     1681 AACACCTCCC TCCAGAATTC AGCACCTTTC TTGACCATAT TTCCTCTTTG GATTATTTTA 
     1741 CAAAACCGGA CTACCAGCTT CTAACATCCG TGTTTGACAA TAGCATCAAG ACCTTTGGAG 
     1801 TAATTGAAAG TGACCCTTTT GACTGGGAGA AGAGTGGAAC TGACGGCTCC CTAACGACCA 
     1861 CCACCACCTC TGCCACCCCT CAGCTGCACA CCCGCCTGAC CCCTGCTGCA ATCGGAATTG 
     1921 CAAATGCCAC CCCTATCCCA GGAGACTTGC TTCGAGAAAA TACAGACGAA GTGTTTCCAG 
     1981 ATGAGCAGCT TAGTGATGGG GAGAATGGAA TCCCTGCTGG TGTGTCCCCA GATAAATTGC 
     2041 CTGGATCTCT GGGGCACCCT CGCCCTCAGG AAAAGGATGT CTGGGAAGAG ATGGACATCA 
     2101 ACAAGAACAA GATAAAGCTC GGAATTTGTA AAGCAGCTAC TGAAGAAGAA AATAGCCATG 
     2161 GTCAAGTGAA TGGAATTCTT AATGCTCCAA GCCTTGGCTC ACCAATTCGT GTCCGATCAG 
     2221 AGATTACTCA GCCAGACAGA GATGTTCCTT TAGTAAGGAA GTTACGTTCC ATTCACAGCT 
     2281 TTGAGCTGGA AAAACGTCTG ACTCTCGAGC CAAAGCCAGA TACTGATAAG TTTCTTGAGA 
     2341 CCTGCATGGA GAAAATGCAG AAAGATTCCA GTGCAGGAAA GGAACCTGTT CTCCCTGCTC 
     2401 TGCCTCATAA GCCTTGTGTC CCTGCTGTGC CCCGCACTGA CCACGTCTGG CACTATGATG 
     2461 AAGAATATCC TCCTGATGCC TCCAAGCCTG CCTCTGCCAA CACCCCAGAG CAGGCCGATG 
     2521 GTGGTGGCAG CCACGGATTT ATAGCTGTTA ACTTAAGCTC TTGTAAGCAG GAGGTTGATT 
     2581 CCAAAGAATG GGTGATTGTG GACAAGGAGC AAGACCTTCG GGACTTTAGG ACAAATGAGG 
     2641 TGTTAGGCCA TAAAACAACT GGAAGCCCTT CAGATGAGGA GCCGGAAGTG CTTCAGGTCC 
     2701 TTGAGGGGTC GCCTCAAGAT GAAAAGATCC AAGTAGGTCC TTGGACTGAC AACCAGCACT 
     2761 TAAAGAAGGA AAGCTCAGGT GTGATTTTAG CCCTTTCTGC TGAATGCCCT GCTACTGCTG 
     2821 CTTCAGAACT GTACACAGAT AGGCTAGACC TCCAGGCTGG AGCTGCAAGT CAGTTCATCA 
     2881 CAGTGACTCC CACAAGTCCC ATGGAGGCAC AAGCAGAAGG ACCCCTGACT GCGATTACAA 
     2941 TTCCTCGACC TTCTGTGGCA TCAACACAGT CAACTTCAGG AAGTTTTCAC TATGGCCCAC 
     3001 AACCAGAGAA GAAAGATCTT CAGCCCTTGG AGCCCACCGT AGAACTCTTC TCTCCAAGGG 
     3061 AGAACTTCTC GGGCTTGGCT GTGACAGAGG GTGAACTAGG AAGCGGAGGA AGCAGAGTGG 
     3121 ATTTGGGACT TCAGATAGAT CACATTGGTC ATGGCATGTT ACCCAACATG AAAGATGGTG 
     3181 ACGCGTCTCA AGACTTAGGA CCAAGAGACC TTCCTGACAG TAATAGATTA GTTGTGAAAG 
     3241 AATTTGAAAA TCTCCCTGGA GAAACAGAAG AGAAAAGCAT TCTTCTGGGC TCAGATAATG 
     3301 AAGATGAGAA GCTAAATAAA GGGCAACACT GTATTGAGAT CTCTCTCCCA GGAGATTTAG 
     3361 TGACCACAGA AAGGGATCAG TCAGCTACCA CAGAACCTCT GGATGTGTCA AAGACACAGA 
     3421 ATTTCAGTGT GGTGCCTAAT CAGGACAAAA ACCATGAGAT AATGAAGCTT TTGGCAGTTG 
     3481 GAACGTCAGA AATGTCTCCA CAAGCCATTG ACCCACATGC TGAAGGGCAG ATAGGCCAGG 
     3541 TGGCAACAGT GCAAAAACAC AAGCTATTTA AAGATGACAC TAAGAGTGAG AGCTTGCCAG 
     3601 GCCAGCAGGG AGACCTTGCT ACTTTTTTGC ACCAAGAGGG TAAGAGAGAG AAAGTTGCCC 
     3661 CTAGAAATGG AGAGCTCTAT CACTGTGTCT CAGAGAGTGA GCATGGGCCT CCTACCCGGA 
     3721 AGGACATGCT TCGGTCGTCC TTGGTGACCA GGCACAGCCG GATCCCTGTT TTAGCACAAG 
     3781 AAATAGACTC AACTTTTGAA TCATCCTCTG CAGTCTCTGC AAAAGAAAAG CTTTTACAGA 
     3841 AGAAAGCCTA TCAGCCAGAA ATAGTCAAAC TTCTGGTAGA AAAAAGGCAA TTCAAGTCTT 
     3901 TCCTCGGGGA CCTCTCAAGT GCCTCTGAGA AGCTGATGGA GGAGAAGCTA GCTGCTGCTG 
     3961 CAGTTCCCTT TTCTGAGGAG GAAGTCTTCG CCCCCTTTTC CAGACTGGCA GTAGACCCCC 
     4021 AGCTGAGTAG GTCAGTTGAA GATGGCTTTC TGTCACCCAT CGTCTCCCAG TCTAGAAAGA 
     4081 GCAAGATTCC GAGGCCAGTA TCCTGGGTCA GCACAGATCA AATCAATAGC TCAGCTTCAC 
     4141 CTCAGTTCTT GCCTCGGCCG CCACCGGGAA AGCCACCAGT AAGGCCTGGA GTAGAAGCCA 
     4201 GGCTACGCAG ATATAAAGTT CTAGGGAGTA GTAACTCTGA TTCAGACCTT TTCTCTCGCC 
     4261 TGGCCCAAAT TCTTCAAAAT GGATCTCAGA AATCCCGGAG TACTACACAA TGCAAGAGCC 
     4321 CAGGATCTCC TCACAATCCA AAAACACCAC CCAAGAGTCC AGTTGTACCT CGAAGGAGTC 
     4381 CCAGTGCCTC TCCTCGAAGC TCTTCCTTGC CTCGAACATC TAGTTCCTCA CCATCTAGGG 
     4441 CTGGACGGCC CCACCATGAC CAGAGGAGTT CTTCCCCACA TCTGGGGAGA AGCAAGTCAC 
     4501 CTCCCAGCCA CTCAGGATCA TCATCCTCCA GGAGGTCCTG CCAACAGGAG CATTGCAAAC 
     4561 CCAGCAAGAA TGGCCCCAAA GGATCTGGCA GCCTCCACCA CCACTCAGCC AGCTCTAAAA 
     4621 CTCCCCCAGG GAAGAGTAAG CCAGCCAGTA AACTCAGCAG AAGCGGACCG ACGCGTACGC 
     4681 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4741 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4801 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4861 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4921 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4981 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5041 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5101 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5161 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5221 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5281 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5341 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5401 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5461 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5521 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5581 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5641 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5701 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5761 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5821 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5881 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5941 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6001 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6061 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6121 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6181 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6241 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6301 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6361 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6421 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6481 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6541 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6601 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6661 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6721 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6781 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6841 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6901 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6961 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7021 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7081 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7141 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7201 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7261 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7321 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7381 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7441 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7501 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7561 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7621 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7681 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7741 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7861 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7921 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7981 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8041 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8101 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8161 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8221 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8281 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8341 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8401 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8461 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8521 TTT
//