LOCUS       RR212005                7590 bp    DNA     circular  SYN 
DEFINITION  Clca2 (Myc-DDK-tagged ORF) - Rat chloride channel calcium activated 2 (Clca2), (10 ug)
ACCESSION   NM_001013202
VERSION     NM_001013202.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3747..3838 
                     /label="Myc-DDK"  
     misc_feature    3868..4453 
                     /label="PolyA signal"  
     misc_feature    3925..3944 
                     /label="Restriction Enzyme"  
     misc_feature    4729..5401 
                     /label="ColE1"  
     misc_feature    5831..6625 
                     /label="Kan/Neo"  
     misc_feature    6660..6962 
                     /label="SV40 ori"  
     misc_feature    7048..7525 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3729..3735 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3729 
                     /label="Clca2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCCAGTG CTGAAGGTCC TTCTGTTCCT CACCCTGCAC CTCTTGCAGG 
     1081 ACACAAAAAG CTTCAAGGTC CATCTCAACA ACAATGGATA TGAGGGTGTG GTCATTGCAA 
     1141 TTAACCCCAG TGTACCAGAG GATGAAAGAC TCATCCCAAG CTTAAAGGAA ATGGTCACTC 
     1201 AAGCCTCTAC TTACCTGTTT GAAGCCAGCC AAGGAAGGTT TTACTTCAGG AATGTAAGCA 
     1261 TATTAGTCCC GATGACCTGG AAGTCAAAAT CTGAGTACTT GATGCCAAAA CGAGAATCAT 
     1321 ATGACAAAGC AGATGTCATA GTTGCCAATT CTCATCTGAA ATATGGAGAC AATCCCTACA 
     1381 CCCTTCAATA TGGGCAGTGT GGGGACAGAG GACGGTACAT ACATTTCACT CCAAACTTCC 
     1441 TGCTCACTGA TAACGTGCGT AACTATGGAC CCCGAGGCAG AGTCTTTGTC CATGAGTGGG 
     1501 CTCATCTCCG GTGGGGAGTG TTTGATGAGT ATAATGAGGA CCGGCCTTTC TACATATCTG 
     1561 GAAAGAACAC TATCGAAGTG ACAAGGTGTT CTACCGACAT CAAGGGCTCT AAGGCGGTCC 
     1621 ATGAGCGCCA GAGAGGCAGC GATGTGACAA GGTTATGCCG ATGGGACTCG AGGACAGGGC 
     1681 TGTATGAACC CAAATGTAAA TTTTTCCCAG ACAAAATTCA GACTGCCAGG GCCTCCATAA 
     1741 TGTTCATGCA AAATCTCAAT TCCGTGGTTG AATTTTGCAC AGAAAAAACC CATAATACGG 
     1801 AAGCCCCAAA CCTACAAAAC AAAATATGCA ATGGCAGAAG CACGTGGGAC GTAATCAAGG 
     1861 AGTCCGCTGA CTTTCAGCAG GCCCCTCCCA TGAGAGGAAC AGAAGCCCCT CCTCCACCCA 
     1921 CGTTTTCCTT GCTCAAGTCC AGACAGCGAG TGATCTGCTT GGTGCTGGAT AAATCCGGAA 
     1981 GCATGGACAC AGAAGACCGC CTTATTCGAA TGAATCAAGC AGCGGAACTG TACTTAACTC 
     2041 AAATTGTTGA AAAGGAATCT ATGGTTGGAT TAGTCACTTT TGACAGCACT GCCCAAATCC 
     2101 AAAATTACCT GATAAAAATA ACTAATACCG GTGACTACAA GAAAATCACT GGAAATCTCC 
     2161 CTCAGCAGGC TGTTGGGGGG ACTTCAATCT GCAGGGGACT GGAAGCAGGA TTTCAGGCAA 
     2221 TTACCTCCAG TGACCAGAGT ACTTCCGGTT CTGAGATTGT ATTGCTAACA GATGGGGAAG 
     2281 ATGATCTAAT AAGTTCATGC TTTGAGGTGG TCAAACACAG CGGTGCGGTC ATTCACACCA 
     2341 TCGCCCTGGG GCCTAAAGCT GCCCGAGAAC TGGAGACTTT GTCGGATATG ACAGGAGGGC 
     2401 TTCGCTTTTA TGCCAACAAA GATGTGAACA GCCTGATGGA CGCTTTCAGT GGGATTTCCT 
     2461 CTGCAAGTGG CAACCTCTCC CAGCAGGCTC TGCAGTTGGA GAGCAAAGCC TTCAATATTG 
     2521 GAGGGGGGAC ATGGATAAAT GGTACAGTAC CTGTGGACAG TACCATTGGC AACGACACTT 
     2581 TCTTTGTTAT TACCTGGACA GTCCAAAAGC CAGAAATTAT TCTTCGAGAT CCAAAAAGAA 
     2641 AAAAATATAC AACCTCAGAT TTCCAAGACA ATAAACTAAA CATTCGATCT GCTCGACTTC 
     2701 AAATACCAGG CACTGCAGAG ACAGGAACTT GGACTTACAG CCTCTTCAAT AAGGGGGTCA 
     2761 AACCTCAGTT GCTAACTGTG ACAGTGACCA CTCGAGCCAG AAGTCCCACC ACACAGCCCC 
     2821 TCATGGCCAC CGCTCACATG AGTCAGAGCA CAGCCCAGTA CCCTAGTCCG ATGATTGTGT 
     2881 ATGCACGGGT CAGCCAAGGG TTTTTGCCTG TTCTGGGAGC CAATGTCACA GCCGTCATAG 
     2941 AATCTGAAAA TGGAAGTCGA GTCACCCTGG AGCTCTGGGA CAACGGGGCA GGTGCTGACA 
     3001 CTGTCAAAAA TGATGGCATC TACACGAGAT ATTTTACAGA TTATCACGGA AATGGTAGAT 
     3061 ACAGCTTAAA AGTGCGTGTC CAGGCAAGAA AGAGCAAAGC CAGACTAAGT TTAAGACAAA 
     3121 AGAACAAGTC TTTATATATA CCTGGCTATG TTGAAAATGG TAAAATTATA CTGAATCCAC 
     3181 CCAGACCGGA AGTCCCAGAA GAAGCTACAG AAGCTGCAGT GGAAGACTTC AGCAGACTAA 
     3241 CCTCTGGAGA GTCGTTTACT GTGTCCGGAG CGCCCCCTGA TGGCGGCCAC GCTCATGTGT 
     3301 TCCCACCCAG TAAAGTTACA GACCTGGAGG CCGAGTTTAA AGGTGATCAT ATTCACCTTA 
     3361 CCTGGACGGC CCCTGGCAAG GTTCTCGATA AAGGAAGAGC GCACAGATAC GTCATCAGAA 
     3421 TGAGCCGGCA TCCTCCTGGT CTCCAAGATT TTAACGACGC TACTTTAGTG AATACTTCCG 
     3481 GTCTGATACC TAAAGAGGCT GGCTCAGAAG AAACGTTTAA ATTCAAACCA GCAAGTTTTA 
     3541 AAATAGAAAA CGGCACCCAG CTCTACATTG CAATTCTGGC CAACAATGAA GCCGGTCTCA 
     3601 CCTCTGAGGT CTCCAACATC GCACAGGCTG TCAACTTTAT TCCTCCAGAA CATAGTTTCT 
     3661 CTACACTCAG CCCTGATATT TCTGCAATCT GTCTGACAAT TTGGGGGTTA ACTGTGATTC 
     3721 TTAACGATAT TTTAAACACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3781 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3841 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3901 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3961 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4021 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4081 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4141 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4201 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4261 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4321 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4381 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4441 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4501 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4561 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4621 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4681 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4741 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4801 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4861 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4921 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4981 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5041 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5101 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5161 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5221 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5281 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5341 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5401 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5461 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5521 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5581 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5641 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5701 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5761 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5821 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5881 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5941 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6001 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6061 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6121 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6181 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6241 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6301 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6361 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6421 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6481 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6541 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6601 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6661 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6721 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6781 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6841 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6901 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6961 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7021 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7081 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7141 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7201 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7261 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7321 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7381 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7441 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7501 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7561 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//