LOCUS       RR211954                8814 bp    DNA     circular  SYN 
DEFINITION  Zfp423 (Myc-DDK-tagged ORF) - Rat zinc finger protein 423 (Zfp423), (10 ug)
ACCESSION   NM_053583
VERSION     NM_053583.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4971..5062 
                     /label="Myc-DDK"  
     misc_feature    5092..5677 
                     /label="PolyA signal"  
     misc_feature    5149..5168 
                     /label="Restriction Enzyme"  
     misc_feature    5953..6625 
                     /label="ColE1"  
     misc_feature    7055..7849 
                     /label="Kan/Neo"  
     misc_feature    7884..8186 
                     /label="SV40 ori"  
     misc_feature    8272..8749 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4953..4959 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4953 
                     /label="Zfp423"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCAGGCGG AAGCAGGCGA AGCCGCGATC GGTGAAAGTT GAAGAGGGGG 
     1081 AGGCCTCGGA CTTCTCGCTG GCCTGGGATT CCTCTGTGGC AGCAGCGGGA GGCCTAGAAG 
     1141 GAGAGTCAGA GTGTGATCGG AAATCCAGCC GTGCGCTGGA AGACAGGAAC AGCGTGACAA 
     1201 GTCAAGAGGA GAGAAACGAG GACGATGAAG ATGTGGAAGA TGAGTCAATT TACACCTGCG 
     1261 ATCACTGTCA GCAGGACTTC GAGTCTCTGG CAGACCTGAC GGACCACCGG GCCCACCGCT 
     1321 GTCCTGGAGA TGGTGATGAC GACCCACAGC TCTCCTGGGT GGCTTCATCT CCCTCCAGCA 
     1381 AGGATGTTGC GTCACCCACG CAGATGATCG GCGATGGTTG TGACCTTGGC CTCGGCGAGG 
     1441 AGGAAGGCGG CACCGGCCTG CCGTACCCTT GCCAGTTCTG CGACAAGTCC TTCATCCGCC 
     1501 TGAGCTACTT GAAGAGGCAT GAACAGATCC ACAGCGACAA GCTGCCGTTC AAGTGCACCT 
     1561 TCTGCAGCCG CCTCTTCAAA CACAAGAGGA GCCGGGACCG GCACATCAAG CTGCACACAG 
     1621 GCGACAAGAA GTACCACTGC CACGAGTGCG AGGCGGCTTT CTCCCGCAGG GACCACCTCA 
     1681 AGATCCACCT GAAGACCCAC AGCTCCAGCA AGCCGTTCAA GTGCAGCGTG TGCAAACGCG 
     1741 GGTTCTCCTC CACCAGCTCC CTGCAGAGCC ACATGCAGGC CCACAAGAAG AATAAGGAAC 
     1801 ACCTGGCTAA GTCAGAGAAG GAAGCCAAGA AGGACGACTT CATGTGTGAC TACTGCGAGG 
     1861 ACACCTTTAG CCAGACAGAA GAGCTGGAGA AGCATGTGCT TACCCTCCAC CCGCAGCTCT 
     1921 CAGAGAAGGC GGACCTCCAG TGTATCCACT GCCCCGAGGT CTTTGTCGAC GAGAGCACGC 
     1981 TGCTGGCCCA CATCCACCAA GCTCACGCCA ACCAGAAACA CAAGTGCCCC ATGTGCCCTG 
     2041 AGCAGTTCTC CTCCGTGGAG GGTGTGTACT GCCACCTGGA CAGCCACCGG CAGCCTGATT 
     2101 CCAGCAATCA CAGTGTCAGC CCCGACCCCG TGCTGGGCAG TGTGGCTTCC ATGAGCAGTG 
     2161 CTACACCTGA CTCGACGCCC GACCCCGTGC TGGGCAGTGT GGCTTCCATG AGCAGTGCTA 
     2221 CACCTGACTC GAGCGCCTCT GTGGAGCGCG GGTCCACGCC AGACTCCACC TTGAAGCCGC 
     2281 TGAGGGGGCA GAAGAAGATG CGGGATGACG GGCAGAGCTG GTCCAAGGTT GTCTACAGCT 
     2341 GCCCCTACTG TTCTAAGCGG GACTTTACCA GCCTGGCTGT GCTAGAGATT CATCTGAAGA 
     2401 CCATTCACGC GGACAAACCT CAGCAGAGTC ACACGTGTCA GATTTGCCTG GACTCCATGC 
     2461 CCACGCTCTA CAACCTCAAC GAGCATGTGC GCAAGCTGCA CAAGAGCCAC GCTTACCCCG 
     2521 TCATGCAATT CGGCAACATC TCCGCCTTCC ACTGCAACTA CTGCCCCGAG ATGTTCGCGG 
     2581 ACATCAACAG CCTGCAGGAG CACATCCGAG TCTCGCACTG TGGCCCCAAT GCCAACCCCC 
     2641 CCGACGGGAA CAATGCTTTC TTCTGTAACC AGTGTTCTAT GGGCTTTCTC ACTGAATCCT 
     2701 CCCTCACAGA GCACATCCAA CAGGCACACT GCAGTGTGGG GAGCACCAAG CTGGAGTCTC 
     2761 CCGTTATCCA GCCCACACAG TCCTTCATGG AGGTCTACTC CTGCCCTTAC TGTACCAACT 
     2821 CCCCTATCTT TGGCTCCATC CTGAAGCTCA CTAAGCACAT CAAAGAGAAC CACAAGAACA 
     2881 TCCCGTTGGC ACACAGCAAG AAGTCCAAGG CGGAGCAGAG TCCGGTCTCC TCTGACGTCG 
     2941 AGGTGTCTTC CCCGAAACGA CAGCGCCTCT CGGGGAGTGC CAACTCCATC TCTAACGGCG 
     3001 AGTACCCCTG CAATCAGTGC GACCTCAAGT TCTCCAACTT CGAGAGCTTC CAGACCCACT 
     3061 TGAAGCTGCA CCTGGAGCTG CTGCTCCGGA AGCAGGCCTG CCCCCAGTGC AAAGAGGACT 
     3121 TCGACTCTCA GGAGTCCCTC CTGCAGCATC TGACCGTGCA CTACATGACC ACGTCCACCC 
     3181 ACTACGTCTG CGAGAGCTGT GACAAGCAGT TCTCCTCAGT GGACGACCTG CAGAAGCACC 
     3241 TGCTGGACAT GCACACCTTT GTGCTATACC ACTGCACCCT GTGTCAGGAG GTCTTCGACT 
     3301 CTAAGGTGTC CATCCAGGTG CACCTGGCCG TGAAGCACAG CAACGAGAAG AAGATGTACC 
     3361 GTTGCACCGC CTGCAACTGG GACTTCCGCA AGGAGGCTGA CCTGCAGGTG CACGTCAAGC 
     3421 ACAGTCACCT CGGCAACCCG GCCAAGGCCC ACAAGTGCAT CTTCTGTGGT GAGACCTTCA 
     3481 GCACCGAGGT GGAGCTCCAG TGCCACATCA CCACGCACAG CAAGAAGTAC AATTGCAGGT 
     3541 TCTGCAGCAA AGCCTTCCAC GCCGTCCTCC TGCTGGAGAA GCACCTTCGG GAGAAGCATT 
     3601 GTGTGTTTGA CCCAGCTGCA GAGAATGGCA CGGCCAACGG GGTGCCCCCC ACCTCCACCA 
     3661 AGAAGGCAGA GCCCGCCGAC CTGCAGGGCA TGCTGCTCAA GAACCCTGAG GCACCGAACA 
     3721 GCCACGAGGC CAGTGAGGAC GATGTGGATG CATCAGAGCC CATGTACGGC TGTGACATCT 
     3781 GTGGTGCAGC CTACACCATG GAGGTGCTGC TGCAGAACCA CCGACTCCGG GATCATAACA 
     3841 TCCGGCCCGG AGAGGACGAT GGCTCACGCA AGAAGGCAGA GTTCATAAAG GGCAGCCACA 
     3901 AGTGTAACGT GTGCTCTCGG ACTTTCTTCT CGGAGAACGG GCTCCGGGAA CACCTGCAGA 
     3961 CGCACCGGGG CCCTGCCAAG CACTACATGT GTCCCATCTG TGGCGAGCGC TTCCCCTCGC 
     4021 TGCTGACGCT CACTGAGCAC AAGGTGACCC ACAGCAAGAG TCTGGACACA GGCACCTGTC 
     4081 GCATCTGCAA AATGCCCCTG CAGAGTGAGG AGGAGTTTAT CGAGCACTGC CAGATGCACC 
     4141 CCGACTTGCG GAACTCCCTC ACTGGTTTCC GCTGTGTGGT CTGTATGCAG ACTGTCACCT 
     4201 CAACCCTGGA GCTCAAGATC CATGGCACCT TTCACATGCA GAAGCTGGCT GGCAGCTCAG 
     4261 CTGCTTCCTC CCCCAATGGC CAGGGGCTGC AGAAGCTCTA CAAGTGCGCC CTGTGCCTCA 
     4321 AAGAGTTCCG TAGCAAGCAG GACCTGGTCA GGCTTGACGT CAATGGGCTG CCCTATGGCC 
     4381 TATGTGCCGG CTGCATGGCC CGTAGTGCCA ATGGACAGGT GGGTGGCCTG GCCCCACCCG 
     4441 AACCTGCCGA CCGGCCCTGC GCTGGCCTCC GCTGCCCTGA ATGTAACGTG AAGTTTGAGA 
     4501 GTGCTGAGGA CCTGGAGAGC CACATGCAGG TGGACCACCG TGATCTTACC CCAGAGACCA 
     4561 GTGGGCCCCG GAAAGGTGCC CAGACGTCAC CAGTGCCCCG GAAGAAGACG TACCAGTGCA 
     4621 TCAAGTGCCA GATGACCTTC GAGAACGAGA GAGAGATCCA GATCCACGTC GCCAACCACA 
     4681 TGATCGAGGA AGGCATCAAC CATGAGTGTA AGCTGTGCAA CCAGATGTTC GACTCCCCAG 
     4741 CCAAGCTCCT TTGTCACCTC ATCGAACACA GCTTCGAGGG CATGGGTGGT ACTTTCAAGT 
     4801 GCCCCGTGTG CTTCACAGTC TTCGTCCAGG CCAACAAGCT GCAACAGCAC ATCTTCGCCG 
     4861 TGCACGGGCA GGAGGACAAA ATCTACGACT GCTCGCAGTG CCCACAGAAG TTTTTCTTCC 
     4921 AGACAGAGTT GCAGAACCAC ACGATGAGCC AGCACGCACA GACGCGTACG CGGCCGCTCG 
     4981 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5041 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5101 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5161 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5221 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5281 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5341 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5401 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5461 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5521 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5581 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5641 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5701 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5761 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5821 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5881 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5941 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6001 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6061 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6121 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6181 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6241 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6301 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6361 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6421 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6481 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6541 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6601 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6661 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6721 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6781 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6841 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6901 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6961 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7021 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7081 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7141 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7201 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7261 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7321 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7381 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7441 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7501 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7561 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7621 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7681 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7741 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7801 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7861 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7921 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7981 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8041 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8101 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8161 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8221 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8281 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8341 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8401 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8461 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8521 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8581 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8641 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8701 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8761 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//