LOCUS       RR211945                7314 bp    DNA     circular  SYN 
DEFINITION  Kif18a (Myc-DDK-tagged ORF) - Rat kinesin family member 18A (Kif18a), (10 ug)
ACCESSION   NM_001137642
VERSION     NM_001137642.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3471..3562 
                     /label="Myc-DDK"  
     misc_feature    3592..4177 
                     /label="PolyA signal"  
     misc_feature    3649..3668 
                     /label="Restriction Enzyme"  
     misc_feature    4453..5125 
                     /label="ColE1"  
     misc_feature    5555..6349 
                     /label="Kan/Neo"  
     misc_feature    6384..6686 
                     /label="SV40 ori"  
     misc_feature    6772..7249 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3453..3459 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3453 
                     /label="Kif18a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAATGGATA GCTGAGGAAG CTGGCTTGGG TTTGAATTTG TGGCTGTGTT 
     1081 TGGAGCGCCT GGAAGCCAAC TCAGCCAAGT TGTCGCGGTC ACTTCTAGTA TTTGCATATG 
     1141 GTGCCACTGG ATCTGGGAAG ACTCACACAA TGCTGGGATC AACTGCTGAA CCCGGGGTCA 
     1201 TGTACCTAAC AATGCTGGAC CTTTTCAAAT GCATGGATGA GGTTAAAGAC GAGAAAGAGT 
     1261 GTAGTACTGC AGTCTCTTAT CTGGAGGTAT ATAATGAACA GATTCGTGAC CTCTTGACAA 
     1321 ACTCAGGGCC ACTTGCTGTC CGGGAAGATG CCCAAAAAGG AGTGGTTGTT CAGGGTCTTA 
     1381 CTCTACACCA GCCTAAATCT TCAGAGGAAA TATTGCAGCT ACTAGACAAT GGAAACAAAA 
     1441 ACAGGACGCA GCATCCCACT GACATGAATG CTGCGTCTTC TCGTTCTCAT GCTGTTTTCC 
     1501 AGATTTATTT ACGACAACAG GACAAAACAG CAAGCATCAA TCAAAATGTC CATATAGCCA 
     1561 AAATGTCACT CATTGACCTC GCAGGATCTG AGCGGGCCAG TATTTCTGGT ACTAAAGGGA 
     1621 CAAGATTTGT AGAAGGCACA AATATTAACA AATCTCTTCT AGCTCTTGGG AATGTCATCA 
     1681 ATGCCTTAGC AGATACAAAG AGAAGAAATC AGCATATTCC TTACAGAAAT AGTAAACTTA 
     1741 CTCGTTTGCT GAAGGATTCT CTTGGAGGAA ACTGTCAAAC TATAATGATA GCTGCTGTTA 
     1801 GTCCTTCCTC TTTATTCTAC GATGACACAT ATAACACTCT TAAGTACGCA AATCGTGCCA 
     1861 AAGACATTAA GTCTTCTCAG TTGAAGAGCA ATGTTGTCAA CCTCAGTAGT CACATATCAC 
     1921 AGTATGTAAA GATCTGCAAC ATGCAGAAGG CAGAGATTTT AATGTTGAAA GAGAAACTAA 
     1981 AGGCCTATGA AGGACAGAAA GCTCTGACTG ACAGAAATGA CTGTGCTAAA TTAGTACATT 
     2041 CAAACGTGGA GGACAAAGAG ATTGAAAGAT TTCAAGAAAT TCTGAACTGC TTGTTCCAGA 
     2101 ATCGAGAAGG AATGAGGCAA GAATATCTGA AGGTAGAAAT GTTACTTAAA GAAAATGAAC 
     2161 TAAAGTCATC CTATCATCAA CAGTGCCATA AGCAAATAGA AATGATGTGT TCTGAGGACA 
     2221 AATTGGAAAA GGCCACTTGC AGACGAGATC ACAGACTTGA AAAGTTGAAA AGCAACCGCT 
     2281 GTGTGCTAGA GAAAAAGAAA GAAGAGATGT TGAAACAGTT TGATGAAAAT ACTAATTGGC 
     2341 TACATCGAGT GGAAAGTGAA ATGCGACTCT TAGGTCAAAA TGGTGATATT CCCGAGGGAC 
     2401 TCAATAAAGA TCTTCATTGT CACCATTTAC ACCTCCAAAA CAAAGACCTG AAAACACAAA 
     2461 TGGGACACAT GATGACTCTG GCTGCTCTCC AGGAGCAGCA GCATGGGCAG ACTGAAGCAG 
     2521 TACTGAATGC TTTGCTTCCA GTCCTAAGAA AACAGTATTG GAGATTGAAA GAAACCGGCC 
     2581 TGTCAAACGC TGCTTTTGAT TCTGACTTCA AAGATATTGA ACATTTAGTA GAGAGAAAAA 
     2641 AGGTAGTGGC TTGGGCTGAC CAAACTGCTG AACATTCAAA CCAAAGTGAT GTTCCTGGTA 
     2701 CTTCTCTTCT TATGACCTTC CCACAGCTTG GACCAATTCA GTCTATTCCT TGTTGCTCAT 
     2761 CCATGAGTGA CCCTAGCATA CTTAAGATTA CTCCACAAAG AAGAACCAAA AGAAAATTAA 
     2821 TACCTTCTCC ATTGAAAGTA AAACATACTC AGAAGTCTGC ACTGTCTGAA AGGACACAGC 
     2881 TTAATGATTC TTTTAGCAAA GAACTTCAGC CTATTGTTTA CACCCCAGAG GACAGCAGAA 
     2941 GAACTGTTCA GGACCTCTCT CCACCCTTAG CAAGAGCGTC CTCACATTCT GCAAATGGAA 
     3001 TGAATGATAA TTCCCTGAAA GCACTGTGTA GAACAGAATC TCCTCTCAGC AGAACCGAGT 
     3061 GTAAAGAGGG TTTGTACTCT ACATCTGCTA TATGTGATGG TATCAAAAGC TTGAAAAATA 
     3121 ATTTGCCTGA ACCACTACCG AGCAGCAAAA GCACTTTACA CAGGGTGGAG TCTTCCTCAT 
     3181 TTCCAACGAA GGATTCTAGG TCTGTATCAA CAAGTGTGCC ATCCTACATG GCGATGACCA 
     3241 CTGCTGCAAA GAGGAAGTGG AAATTCATGA GTTCTACATC AGATGCTTCA TTAAAATCTG 
     3301 ATGAAAGTTG TGGACTTGCC AAACGCATTC GACAGGATAA TTCAAGTGTT AAGCTCATCC 
     3361 GAGAAAACAG ACTGAGAGTG GGCTATAAAA AAACCACCAA CAAAACAAAT TCAAACATGC 
     3421 TTAGAAAATT TAGAAGAAAC ACTTCAAAAG AAAATGTACA AACGCGTACG CGGCCGCTCG 
     3481 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3541 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3601 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3661 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3721 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3781 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3841 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3901 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3961 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4021 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4081 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4141 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4201 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4261 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4321 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4381 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4441 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4501 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4561 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4621 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4681 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4741 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4801 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4861 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4921 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4981 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5041 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5101 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5161 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5221 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5281 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5341 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5401 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5461 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5521 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5581 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5641 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5701 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5761 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5821 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5881 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5941 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6001 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6061 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6121 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6181 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6241 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6301 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6361 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6421 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6481 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6541 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6601 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6661 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6721 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6781 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6841 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6901 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6961 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7021 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7081 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7141 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7201 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7261 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//