LOCUS       RR211917                7344 bp    DNA     circular  SYN 
DEFINITION  Capn3 (Myc-DDK-tagged ORF) - Rat calpain 3 (Capn3), (10 ug)
ACCESSION   NM_017117
VERSION     NM_017117.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3501..3592 
                     /label="Myc-DDK"  
     misc_feature    3622..4207 
                     /label="PolyA signal"  
     misc_feature    3679..3698 
                     /label="Restriction Enzyme"  
     misc_feature    4483..5155 
                     /label="ColE1"  
     misc_feature    5585..6379 
                     /label="Kan/Neo"  
     misc_feature    6414..6716 
                     /label="SV40 ori"  
     misc_feature    6802..7279 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3483..3489 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3483 
                     /label="Capn3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGACCGTT ATTAGTCCAA CTGTGGCCCC AAGGACAGGA GCTGAGCCCA 
     1081 GGTCCCCAGG GCCAGTTCCT CACCCAGCTC AAGGCAAGAC CACTGAGGCT GGAGGTGGAC 
     1141 ACCCGGGTGG CATCTATTCA GCCATCATCA GCCGCAATTT TCCGATCATT GGTGTGAAAG 
     1201 AGAAGACATT CGAGCAGCTC CACAAGAAGT GCCTAGAGAA GAAAGTTCTT TACCTGGATC 
     1261 CCGAGTTCCC ACCGGATGAG ACCTCTCTCT TTTACAGCCA GAAGTTCCCC ATCCAGTTCG 
     1321 TCTGGAAGAG ACCTCCGGAA ATTTGTGAGA ATCCCCGATT TATCATTGGT GGAGCCAACA 
     1381 GGACTGACAT CTGCCAAGGA GATCTAGGGG ACTGCTGGCT TCTTGCAGCC ATTGCCTGTC 
     1441 TGACCCTGAA TGAGCGACTG CTTTTCCGAG TTATACCTCA TGATCAAAGT TTCACTGAAA 
     1501 ACTACGCAGG GATCTTCCAC TTCCAGTTCT GGCGCTATGG AGACTGGGTA GATGTGGTTA 
     1561 TTGACGACTG TCTGCCGACA TACAACAACC AGCTGGTCTT CACCAAATCC AACCACCGCA 
     1621 ATGAGTTCTG GAGTGCTCTA CTGGAGAAAG CATATGCCAA GCTCCATGGT TCCTATGAAG 
     1681 CTCTGAAAGG TGGGAACACC ACAGAAGCCA TGGAGGACTT CACAGGAGGG GTGACAGAGT 
     1741 TTTTTGAGAT CAAGGATGCT CCGAGTGACA TGTACAAGAT CATGAGGAAA GCTATCGAGA 
     1801 GAGGCTCCCT CATGGGCTGC TCCATTGATG ATGGCACCAA CATGACTTAT GGAACCTCTC 
     1861 CTTCTGGTCT GAACATGGGG GAATTGATTG CGCGGATGGT GAGAAATATG GATAACTCGC 
     1921 TGCTCAGAGA CTCAGACCTG GACCCCAGGG CCTCAGATGA CAGACCGTCA CGGACAATTG 
     1981 TTCCGGTGCA GTATGAAACA AGAATGGCCT GTGGACTGGT GAAAGGGCAC GCCTATTCAG 
     2041 TCACTGGGCT GGAGGAGGCC CTGTTCAAAG GCGAGAAGGT GAAGCTGGTG CGGCTGCGGA 
     2101 ACCCCTGGGG CCAGGTGGAG TGGAACGGCT CTTGGAGTGA TGGTTGGAAG GACTGGAGCT 
     2161 TTGTGGACAA AGACGAGAAG GCCCGTCTGC AGCACCAGGT CACCGAGGAT GGAGAGTTCT 
     2221 GGATGTCATA TGATGACTTT GTCTACCATT TCACAAAGCT GGAGATCTGC AACCTCACAG 
     2281 CTGATGCCCT GGAGTCCGAT AAGCTTCAGA CCTGGACAGT GTCTGTAAAT GAGGGCCGCT 
     2341 GGGTGAGGGG CTGTTCTGCT GGAGGCTGCC GGAACTTCCC AGACACTTTC TGGACCAACC 
     2401 CGCAGTACCG TCTCAAGCTC CTGGAGGAGG ATGATGACCC TGATGACTCT GAGGTGATTT 
     2461 GCAGCTTCCT CGTGGCTCTG ATGCAGAAAA ATCGGCGCAA GGACCGGAAG CTGGGGGCCA 
     2521 ACCTCTTCAC CATTGGCTTC GCTATCTACG AGGTTCCCAA AGAGATGCAC GGGAATAAGC 
     2581 AACACCTGCA GAAGGACTTC TTCTTGTACA ATGCCTCCAA GGCCAGGAGC AAAACCTACA 
     2641 TCAACATGCG GGAGGTGTCC CAGCGCTTCC GCCTGCCGCC CAGCGAGTAT GTCATTGTCC 
     2701 CCTCCACTTA CGAGCCCCAT CAGGAGGGGG AATTCATCCT CCGGGTCTTC TCTGAAAAGA 
     2761 GGAATCTCTC TGAGGAAGCT GAGAATACAA TCTCTGTGGA CCGGCCAGTG AAAAAGAAAA 
     2821 AAAACAAGCC CATCATCTTC GTTTCAGACA GAGCAAACAG CAACAAGGAG CTGGGTGTGG 
     2881 ACCAGGAGGC AGAGGAGGGC AAAGACAAAA CAGGGCCGGA TAAACAAGGG GAAAGCCCAC 
     2941 AGCCACGGCC TGGCCACACA GACCAGGAGA GTGAGGAGCA GCAGCAATTC CGGAACATCT 
     3001 TCAGGCAGAT TGCAGGCGAC GACATGGAGA TCTGTGCGGA TGAACTCAAG AATGTCCTTA 
     3061 ATACGGTGGT GAACAAACAC AAGGACCTGA AGACACAAGG GTTCACTCTG GAGTCCTGCA 
     3121 GAAGCATGAT AGCTCTCATG GATACAGATG GCTCTGGGAG ACTGAATCTT CAAGAGTTCC 
     3181 ATCACCTCTG GAAAAAGATC AAGGCCTGGC AGAAAATCTT CAAACACTAT GACACTGACC 
     3241 ATTCTGGTAC CATCAATAGC TATGAGATGC GAAATGCAGT CAATGATGCA GGCTTCCATC 
     3301 TCAACAGCCA ACTCTATGAC ATCATCACCA TGCGCTACGC AGACAAACAC ATGAACATCG 
     3361 ACTTTGACAG CTTCATCTGC TGCTTCGTCA GGCTGGAAGG GATGTTCAGA GCTTTTCACG 
     3421 CATTTGACAA GGATGGAGAT GGCATCATCA AACTGAACGT ACTTGAGTGG CTGCAGCTTA 
     3481 CCATGTATGC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3541 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3601 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3661 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3721 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3781 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3841 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3901 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3961 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4021 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4081 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4141 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4201 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4261 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4321 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4381 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4441 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4501 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4561 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4621 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4681 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4741 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4801 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4861 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4921 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4981 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5041 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5101 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5161 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5221 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5281 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5341 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5401 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5461 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5521 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5581 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5641 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5701 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5761 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5821 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5881 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5941 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6001 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6061 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6121 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6181 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6241 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6301 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6361 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6421 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6481 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6541 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6601 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6661 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6721 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6781 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6841 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6901 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6961 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7021 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7081 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7141 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7201 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7261 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7321 TAACAAAAAT TTAACGCGAA TTTT
//