LOCUS       RR211872                8307 bp    DNA     circular  SYN 
DEFINITION  Kcnt2 (Myc-DDK-tagged ORF) - Rat potassium channel, subfamily T, member 2 (Kcnt2), (10 ug)
ACCESSION   NM_198762
VERSION     NM_198762.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4464..4555 
                     /label="Myc-DDK"  
     misc_feature    4585..5170 
                     /label="PolyA signal"  
     misc_feature    4642..4661 
                     /label="Restriction Enzyme"  
     misc_feature    5446..6118 
                     /label="ColE1"  
     misc_feature    6548..7342 
                     /label="Kan/Neo"  
     misc_feature    7377..7679 
                     /label="SV40 ori"  
     misc_feature    7765..8242 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4446..4452 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4446 
                     /label="Kcnt2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTGATTTG GAGAGCGAAG TACCCCCTCT GCCTCCCAGG TACAGATTCC 
     1081 GTGATTTGCT GCTAGGGGAC CAAGGCTGGC AGAATGATGA CAGGGTTCAA GTCGAATTCT 
     1141 ACATGAATGA AAACACATTT AAAGAGAGAT TAAAGTTGTT TTTCATAAAA AACCAGCGAT 
     1201 CAAGTCTAAG AATCCGCTTG TTCAACTTTT CCCTCAAATT GTTAAGCTGT TTGTTATACA 
     1261 TTATCCGTGT GTTGCTTGAG AAACCTTCAC AGGGAAATGA TTGGTCTCAC ATATTTTGGG 
     1321 TTAACAGAAG CCTGCCTTTA TGGGGTTTAC AGGTCTCGGT GGCATTGATA AGTCTATTTG 
     1381 AAACAATACT CCTTGGATAT CTCAGTTATA AGGGGAATAT CTGGGAGCAG ATTTTACGAG 
     1441 TTCCATTCAT CTTGGAAATA ATCAATGCAG TTCCCTTCAT CATCTCAATA TTCTGGCCTA 
     1501 CGTTAAGAAA TCTCTTTGTT CCAGTTTTTC TAAATTGTTG GCTTGCCAAA CATGCCTTGG 
     1561 AAAATATGAT TAATGATCTT CATCGAGCCA TTCAGCGTAC ACAGTCTGCA ATGTTTAATC 
     1621 AAGTTCTGAT TTTGATATCT ACTTTACTAT GCCTTATCTT CACTTGCATT TGTGGAATTC 
     1681 AGCATCTGGA ACGAATTGGA AAGAAACTCA ATCTCTTTGA CTCCCTGTAT TTCTGCATTG 
     1741 TGACATTTTC TACTGTGGGC TTTGGGGATG TTACTCCTGA AACATGGTCC TCCAAGCTTT 
     1801 TTGTTGTTGC TATGATCTGC GTTGCTCTTG TAGTTCTCCC CATACAGTTT GAACAGCTGG 
     1861 CCTATTTGTG GATGGAGAGA CAAAAGTCGG GAGGAAACTA TAGTCGACAC AGAGCTCAGA 
     1921 CTGAGAAGCA TGTTGTTCTG TGTGTCAGCT CATTGAAGAT TGACTTGCTT ATGGATTTTT 
     1981 TGAATGAATT CTATGCACAT CCAAGACTGC AGGATTATTA TGTGGTGATT TTGTGTCCCA 
     2041 CGGAGATGGA TGTGCAAGTT CGGAGAGTGC TACAGATTCC AATGTGGTCC CAGAGAGTGA 
     2101 TCTACCTTCA AGGCTCTGCC CTTAAAGACC AGGATCTCCT GAGAGCAAAG ATGGACAATG 
     2161 CTGAGGCCTG TTTCATTTTG AGTAGCCGCT GTGAAGTCGA TAGAACATCA TCTGATCACC 
     2221 AGACAATTTT GAGAGCATGG GCTGTAAAAG ATTTTGCCCC AAATTGTCCT CTGTATGTTC 
     2281 AGATATTAAA ACCAGAAAAT AAATTCCACA TCAAATTTGC AGATCATGTT GTTTGTGAAG 
     2341 AAGAGTTTAA GTACGCCATG TTAGCTTTAA ACTGTATATG CCCAGCAACA TCTACACTTA 
     2401 TTACACTACT GGTTCATACC TCTAGAGGGC AGTGTGTGTG CTTGTGTTGC AGAGAAGGGC 
     2461 AGCAGTCACC TGAACAGTGG CAGAAGACGT ATGGGAGGTG CTCAGGAAAT GAAGTCTACC 
     2521 ACATCGTTCT GGAAGAAAGT ACATTTTTTG CTGAATATGA AGGGAAGAGT TTTACCTATG 
     2581 CTTCTTTCCA TGCACACAAA AAGTTTGGTG TCTGCTTGGT TGGTGTTAGG AGGGAGGATA 
     2641 ATAAAAACAT TTTGCTGAAT CCAGGTCCTC GATACATTAT GAATGCTTCA GACATATGTT 
     2701 TTTATATTAA TATTACCAAA GAAGAAAATT CAGCATTTAA GAATCAAGAC CAACAGAGAA 
     2761 AAAGTAATGT GTCAAGGTCA TTTTATCATG GGCCTTCCAG ATTGCCTGTC CACAGTATCA 
     2821 TTGCCAGCAT GGGTACTGTG GCTATAGACT TACAAGACAC AAGCTGTAGA GCAGCAAGTG 
     2881 GCCCTACACT GGCTCTTCCT TCAGAGGGAG GCAAGGAACT AAGAAGACCT AGCATTGCTC 
     2941 CTGTTTTAGA GGTAGCAGAT ACATCATCAA TTCAAACCTG CGATCTTCTA AGTGACCAGT 
     3001 CAGAAGATGA AACTACACCA GATGAAGAAA CATCTTCAAA TTTAGAGTAT GCCAAAGGCT 
     3061 ACCCACCTTA TTCTCCATAC ATAGGAAGTT CACCTACTTT TTGTCATCTA CTTCAAGAAA 
     3121 AAGTGCCCTT TTGCTGCCTA AGATTAGACA AGAGTTGCCA GCATAACTAC TATGAGGATG 
     3181 CAAAAGCCTA TGGATTCAAA AATAAACTAA TTATAGTTGC AGCTGAAACG GCTGGGAACG 
     3241 GATTATATAA TTTTATTGTA CCTCTCAGGG CATATTATAG ACCAAAGAAA GAATTAAATC 
     3301 CCATAGTTCT TCTATTGGAT AATCCGCCAG ATATGCATTT TCTGGATGCA ATCTGTTGGT 
     3361 TTCCAATGGT TTACTACATG GTGGGCTCTA TTGACAACCT AGATGATTTG CTCAGGTGTG 
     3421 GAGTGACCTT CGCTGCCAAC ATGGTGGTTG TGGACAAAGA AAGCACCATG AGTGCAGAGG 
     3481 AAGACTACAT GGCAGATGCC AAGACGATTG TGAATGTGCA GACTCTTTTC AGGTTGTTTT 
     3541 CGAGTCTCAG TATTATCACT GAGCTTACTC ATCCAGCAAA TATGAGATTC ATGCAATTCA 
     3601 GAGCCAAGGA CTGTTACTCT CTTGCACTTT CAAAACTGGA AAAGAAAGAG CGAGAACGAG 
     3661 GTTCTAACCT GGCCTTCATG TTCCGACTGC CTTTCGCTGC GGGGAGAGTG TTCAGCATCA 
     3721 GTATGCTGGA CACGCTGCTT TATCAGTCAT TTGTGAAAGA TTATATGATT TCTATCACCA 
     3781 GACTTCTTTT GGGACTGGAC ACCATACCAG GATCGGGGTT TCTTTGTTCT ATGAAAATTA 
     3841 CTGAAGATGA CTTGTGGATC AGAACGTATG CCAGACTCTA TCAGAAGCTG TGTTCTTCTA 
     3901 CTGGAGATGT TCCCATTGGG ATCTACAGAA CAGAATCTCA GAAGCTAACT ACATCTGAGT 
     3961 CTCAAATATC CATCAGTGTG GAAGAGTGGG AAGACACCAA AGATGTCAAA GACCCAGGGC 
     4021 ACCACCGCAG CATTCACCGC AACTCAACCT CTAGCGACCA GTCAGACCAC CCCTTGCTGC 
     4081 GGAGGAAGAG CATGCAGTGG GCCCGGAGGC TGAGCAGAAA AGGGCCAAAG CACTCTGGTA 
     4141 AAACTGCAGA AAAGATAACC CAGCAACGAC TGAATCTCTA CAGGAGGTCG GAAAGACAGG 
     4201 AGCTTGCTGA ACTTGTGAAA AACAGAATGA AACACCTGGG CCTCTCTACG GTGGGCTACG 
     4261 ATGAAATGAA TGATCATCAG AGCACCCTTT CCTACATCCT GATTAATCCA TCTCCAGACA 
     4321 CCAGGCTTGA GCTGAATGAT GTTGTATATT TGATCCGACC AGATCCACTG TCCTACCTTC 
     4381 CGAACAGTGA ACCCAGTAGG AAAAACAGCA TCTGCAATGC CGCTGTTCAA GATTCTCGGG 
     4441 AGGAAACACA ACTCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4501 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4561 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4621 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4681 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4741 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4801 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4861 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4921 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4981 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5041 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5101 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5161 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5221 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5281 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5341 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5401 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5461 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5521 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5581 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5641 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5701 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5761 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5821 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5881 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5941 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6001 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6061 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6121 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6181 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6241 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6301 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6361 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6421 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6481 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6541 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6601 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6661 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6721 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6781 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6841 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6901 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6961 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7021 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7081 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7141 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7201 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7261 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7321 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7381 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7441 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7501 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7561 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7621 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7681 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7741 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7801 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7861 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7921 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7981 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8041 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8101 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8161 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8221 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8281 ATTTAACAAA AATTTAACGC GAATTTT
//