LOCUS       RR211862                6906 bp    DNA     circular  SYN 
DEFINITION  Chgb (Myc-DDK-tagged ORF) - Rat chromogranin B (Chgb), (10 ug)
ACCESSION   NM_012526
VERSION     NM_012526.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3063..3154 
                     /label="Myc-DDK"  
     misc_feature    3184..3769 
                     /label="PolyA signal"  
     misc_feature    3241..3260 
                     /label="Restriction Enzyme"  
     misc_feature    4045..4717 
                     /label="ColE1"  
     misc_feature    5147..5941 
                     /label="Kan/Neo"  
     misc_feature    5976..6278 
                     /label="SV40 ori"  
     misc_feature    6364..6841 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3045..3051 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3045 
                     /label="Chgb"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCGGGCC ATGCTCCTCG GCCTCCTGGG CGCAGCAGCT CTGGCTGCTG 
     1081 TCATCTCAGC TCCAGTGGAT AACAGGGATC ACAATGAAGA AATGGTGACT CGCTGCATTA 
     1141 TTGAAGTCCT CTCAAATGCC CTATCCAAGT CCAGTGCTCC AACCATCACC CCTGAGTGCC 
     1201 GGCAAGTCCT GAGGAAGAGT GGAAAAGAGG TCAAAGGTGA AGAGAAAGGT GAAAATGAGA 
     1261 ACTCGAAGTT TGAAGTACGG TTGCTAAGAG ACCCATCTGA TGCCTCAGTA GGCCGCTGGG 
     1321 CCTCCAGTAG GGAGGAAACA GGGGCTCCAG TAGAAGACTC TCCAGGTCAG GCGAAGGTAG 
     1381 ACAATGAGGA GTGGACAGGA GGAGGAGGAC ACAGTCGAGA GGCAGTAGAT GACCAGGAGA 
     1441 GCCTCCATCC CTCCAACCAA CAAGTGTCCA AAGAAGCAAA GATACGCCAT TCGGAGGAGA 
     1501 GGGGCGGAAA AGAGGAGGAG GAGGAAGAAG GCAAGATTTA TCCAAAGGGA GAGCATAGGG 
     1561 GAGATGCTGG TGAGGAGAAG AAACACACTG AAGAATCTGG AGAGAAACAC AACGCCTTCT 
     1621 CCAACAAAAG AAGCGAAGCT TCAGCTAAGA AAAAAGAGGA GTCAGTGGCC AGAGCAGAGG 
     1681 CACACTTCGT GGAGCTGGAG AAGACACACA GCAGGGAGCA GAGCAGCCAG GAGAGTGGAG 
     1741 AGGAGACGAG GAGGCAGGAG AAACCACAGG AGCTTCCCGA CCAGGACCAG AGCGAGGAAG 
     1801 AATCCGAGGA AGGCGAGGAA GGCGAGGAAG GCGCAACCTC AGAAGTAACC AAACGCAGGC 
     1861 CCAGGCACCA CCACTGGAGA AGCCAGTCCA ACAAGCCCTC TTACGAAGGG CGTCGTCCTC 
     1921 TGTCTGAGGA AAGGAAACAT GCTGCTGGGG AGTCCAAGGA TGCAAATGTG GCCACAGCCA 
     1981 ATTTAGGGGA AAAAAGGGGC CACCACCTAG CTCACTACCG GGCTTCAGAG GAAGAACCTG 
     2041 ACTATGGGGA AGAATTGAGA AGCTATCCGG GCTTCCAGGC TCCCCAGGGA CTGCAGTACC 
     2101 AGGGCAGAGG AAGTGAAGAG GTCAGAGCCC CAAGCCCTCG GAGTGAGGAG AGCCAGGAAA 
     2161 AGGAGTACAA GAGAAACCAC CCCGACTCTG AACTGGAAAG CACAGCAAAC AGACACAGTG 
     2221 AAGAAACTGA GGAAGAGAGG AGCTACGAGG GGGCAAAGGG ACGACAACAC AGAGGCAGGG 
     2281 GGCGGGAGCC AGGAGCTTAT CCTGCTCTTG ACAGCAGACA AGAAAAAAGG CTCCTGGATG 
     2341 AAGGGCATGA CCCTGTTCAT GAAAGCCCAG TAGATACGGC AAAAAGGTAT CCACAAAGCA 
     2401 AATGGCAGGA ACAGGAGAAA AACTACCTCA ACTATGACGA GGAAGGGGAC CAAGGAAGAT 
     2461 GGTGGCAGCA GGAAGAGCAG CTAGAACCGG AAGAAAGCAG GGAGGAAGTG AGCTTTCCAG 
     2521 ACAGACAATA CGCGCCCTAC CCCACCACTG AAAAGAGGAA GAGGCTAGGG GCGCTGTTCA 
     2581 ACCCGTACTT TGACCCTCTC CAGTGGAAGA ATAGCGACTT TGAGAAAAAA GGCAACCCAG 
     2641 ATGACAGTTT CCTTGATGAT GACGGCGAAG ACGGAAATGG GGTGACAATG ACTGAGAAGA 
     2701 ATTTCTTCCC AGAGTACAAC TATGACTGGT GGGAGAAAAG GCCCTTCTCG GAGGATGTGA 
     2761 ATTGGGGATA TGAGAAGAGA AGCTTTGCCA GGGCCCCTCA CTTGGACTTG AAAAGGCAAT 
     2821 ACGACGATGG AGTGGCTGAA CTGGACCAGC TCCTTCACTA CAGGAAGAAG GCAGCCGAAT 
     2881 TTCCCGACTT CTACGATTCA GAGGAGCAGA TGGGGCCTCA CCAGGAGGCA GAAGATGAAA 
     2941 AGGACAGGGC TGACCAGAGA GTTCTGACCG AGGAAGAGAA AAAGGAACTG GAGAACTTGG 
     3001 CGGCCATGGA TCTGGAACTG CAGAAAATAG CGGAAAAGTT CAGCCAGCGG GGCACGCGTA 
     3061 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3121 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3181 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3241 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3301 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3361 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3421 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3481 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3541 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3601 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3661 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3721 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     3781 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     3841 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     3901 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     3961 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4021 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4081 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4141 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4201 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4261 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4321 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4381 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4441 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4501 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4561 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4621 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4681 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     4741 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     4801 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     4861 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     4921 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     4981 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5041 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5101 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5161 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5221 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5281 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5341 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5401 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5461 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5521 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5581 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5641 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     5701 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     5761 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     5821 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     5881 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     5941 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6001 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6061 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6121 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6181 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6241 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6301 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6361 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6421 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6481 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6541 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6601 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6661 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     6721 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     6781 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     6841 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     6901 AATTTT
//