LOCUS       RR211835                8778 bp    DNA     circular  SYN 
DEFINITION  Nrcam (Myc-DDK-tagged ORF) - Rat neuronal cell adhesion molecule (Nrcam), (10 ug)
ACCESSION   NM_013150
VERSION     NM_013150.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4935..5026 
                     /label="Myc-DDK"  
     misc_feature    5056..5641 
                     /label="PolyA signal"  
     misc_feature    5113..5132 
                     /label="Restriction Enzyme"  
     misc_feature    5917..6589 
                     /label="ColE1"  
     misc_feature    7019..7813 
                     /label="Kan/Neo"  
     misc_feature    7848..8150 
                     /label="SV40 ori"  
     misc_feature    8236..8713 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4917..4923 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4917 
                     /label="Nrcam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGAAGAAG AAGCCCTTGT CTGCAGGCAG AGCGCCCCTG TTTCTCTTCC 
     1081 TGTGCCAGAT GATCAGCGCT CTGGATGTTC CTCTTGATCC AAAGCTCCTT GATGACTTGG 
     1141 TACAGCCTCC AACTATCACT CAACAGTCAC CAAAAGACTA CATCATTGAC CCACGGGAGA 
     1201 ATATTGTAAT CCAATGTGAG GCCAAAGGGA AACCTCCTCC AAGCTTTTCC TGGACTCGTA 
     1261 ACGGAACACA TTTTGACATA GACAAAGACC CTCTGGTCAC TATGAAGCCT GGCTCAGGAA 
     1321 CCCTTGTCAT CAACATCATG AGTGAAGGAA AGGCGGAGAC CTATGAAGGG GTTTACCAGT 
     1381 GCACTGCAAG GAATGAGCGC GGAGCTGCTG TCTCCAATAA CATTGTTGTC CGCCCCTCTA 
     1441 GGTCACCCTT GTGGACCAAG GAAAGACTTG AACCAATAAT CCTCCGAAGT GGTCAGTCAC 
     1501 TAGTACTACC ATGTAGGCCT CCAATTGGAT TACCACCGGC CATAATATTT TGGATGGATA 
     1561 ACTCCTTTCA AAGACTGCCA CAGAGTGAGC GGGTTTCCCA AGGACTGAAT GGAGACCTTT 
     1621 ACTTCTCCAA TGTCCTCCCA GAGGACACCC GTGAGGACTA CATCTGCTAT GCCAGATTTA 
     1681 ATCACACTCA AACAATTCAA CAGAAACAAC CTATTTCTCT GAAGGTGATT TCAGTGGATG 
     1741 AATTGAATGA CACTATAGCT GCTAATTTGA GTGACACTGA GTTTTATGGT GCTAAATCTA 
     1801 GTAAAGAGAG GCCACCAACA TTTCTAACTC CAGAGGGCAA TGAAAGTCAC AAGGAAGAAT 
     1861 TAAGAGGAAA CGTGCTTTCC CTGGAGTGCA TTGCAGAAGG CCTACCTACT CCAGTTATTT 
     1921 ACTGGATCAA GGAAGATGGA ACGCTTCCTG TCAACCGGAC GTTTTATCGG AACTTTAAGA 
     1981 AAACCTTGCA GATCATTCAT GTCTCTGAAG CAGACTCTGG AAATTATCAG TGCATAGCAA 
     2041 AAAACGCATT GGGAGCCGTC CATCATACCA TTTCTGTCAC AGTTAAAGCG GCTCCCTACT 
     2101 GGATTGTTGC ACCTCACAAC CTCGTGCTTT CCCCAGGGGA GAATGGGACC CTCATCTGCA 
     2161 GAGCTAACGG CAACCCAAAA CCCAGAATTA GCTGGTTAAC AAATGGAGTC CCAGTAGAAA 
     2221 TTGCTCTCGA TGACCCCAGC CGAAAAATCG ATGGTGATAC CATTATGTTT TCAAATGTTC 
     2281 AAGAAAGCTC AAGTGCGGTT TATCAGTGCA ATGCCTCTAA CAAATATGGA TATTTACTAG 
     2341 CAAATGCATT TGTAAATGTG CTCGCTGAAC CACCTCGGAT TCTTACCTCA GCAAACACAC 
     2401 TGTACCAGGT CATTGCAAAC CGGCCTGCTT TGCTGGACTG TGCCTTCTTT GGGTCTCCTA 
     2461 TGCCTACCAT TGAGTGGTTT AAAGGAACTA AAGGAAGCGC TCTTCACGAA GACATTTATG 
     2521 TTTTACATGA TAACGGAACG CTAGAAATTC CTGTGGCCCA AAAGGATAGT ACAGGGACAT 
     2581 ATACTTGTGT CGCAAGGAAT AAATTAGGGA TGGCAAAGAA TGAAGTTCAC TTGGAAATCA 
     2641 AAGATCCAAC CAGGTTCATT AAACAACCTG AATATGCAGT CGTTCAGAGG GGGAGCAAGG 
     2701 TTTCCTTTGA ATGCAAAGTG AAACACGACC ACACCTTAAT CCCCACCATT CTGTGGCTGA 
     2761 AGGACAATGG AGAGCTGCCC AATGATGAAA GGTTCTCTGT CGACAAGGAC CATCTGGTGG 
     2821 TCTCTGACGT AAAGGATGAA GATGGTGGGA CCTACACATG TGCAGCCAAC ACAACGCTGG 
     2881 ACAGTGTTTC AGCCAGCGCC GTGCTCAGGG TGGTCGCTCC TACTCCAACT CCAGCCCCCA 
     2941 TTTACGATGT CCCGAATCCT CCCTTTGATT TAGAATTGAC CAATCAACTT GACAAAAGTG 
     3001 TTCAGCTGAC ATGGACCCCA GGCGATGACA ACAATAGTCC CATTACAAAA TTCATCATCG 
     3061 AGTATGAAGA TGCAATGCAT GAGGCAGGGC TGTGGCGCCA CCAGGCGGAA GTTTCTGGAA 
     3121 CGCAGACCAC AGCCCAGCTG AAGCTGTCTC CCTATGTGAA CTACTCCTTC CGAGTCATGG 
     3181 CGGAGAACAG CATCGGGAGG AGTGTGCCAA GCGAGGCATC GGAGCAGTAT CTTACAAAGG 
     3241 CCGCGGAACC AGACCAGAAT CCCACGGCTG TGGAAGGACT AGGGACAGAG CCGGACAACT 
     3301 TGGTGATCAC ATGGAAGCCC CTGAATGGTT TTCAATCGAA TGGGCCCGGC CTCCAGTACA 
     3361 AAGTGAGCTG GCGTCAGAAA GATGGTGACG ATGAATGGAC GTCTGTGGTT GTGGCCAATG 
     3421 TATCCAAATA CATTGTTTCT GGCACACCAA CTTTTGTCCC ATACCTGATA AAAGTTCAAG 
     3481 CCCTGAATGA TGTGGGCTTT GCTCCAGAGC CAGCTGCAGT CATGGGACAT TCCGGAGAAG 
     3541 ACCTTCCAAT GGTGGCTCCT GGAAATGTGC GTGTCAGTGT GGTGAACAGC ACCTTGGCAG 
     3601 AGGCACACTG GGACCCTGTA CCTCCCAAGA GTGTCCGAGG ACACTTACAA GGCTACCGGA 
     3661 TTTACTACTG GAAGGCTCAG AGCTCATCTA AAAGAAACAG ACGCCACATT GAGAAGAAGA 
     3721 TCCTCACCTT CCAGGGCAGC AAGACTCACG GCATGCTGCC AGGGCTGCAA CCTTACAGTC 
     3781 ACTATGTTCT CAATGTCCGA GTGGTCAATG GGAAAGGGGA GGGCCCAGCC AGCGCCGACA 
     3841 GAGGCTTCCA TACTCCAGAG GGAGTCCCTA GTGCTCCCTC ATCTTTGAAA ATTGTTAATC 
     3901 CTACACTGGA CTCTCTCACT TTGGAATGGG ACCCTCCAAG CCACCCAAAT GGTATCCTGA 
     3961 CTGAATACAT CTTAAAATAT CAGCCAATTA ATAGCACACA CGAACTAGGC CCTCTGGTAG 
     4021 ATTTAAAAAT TCCTGCCAAC AAGACACGCT GGACTTTGAA AAATTTAAAT TTCAGCACTC 
     4081 GGTACAAGTT CTACTTCTAT GCACAAACAT CAGTGGGATC GGGCAGTCAG ATCACAGAGG 
     4141 AAGCAATAAC AACTGTGGAC GAAGCTGGTA TTCTTCCACC TGATGTAGGT GCAGGCAAAG 
     4201 TCCGTGCAGT AAGTCCCAGG ATCGGCAATG TCACTGCTGC AGCTGCTGAG ACGTATGCCA 
     4261 ATATCAGTTG GGAATATGAG GGACCAGAGC ATGTGAAGTT TTATGTTGAA TATGGTGTAG 
     4321 CAGGCAGCAA AGAAGAATGG AGGAAAGAGA TTGTAAATGG TTCTCGGAGC TTCTTTGGGT 
     4381 TAAAGGGTCT AATGCCAGGA ACAGCATACA AAGTCCGAGT TGGTGCTGAG GGGGACTCTG 
     4441 GTTTTGTGAG TTCAGAGGAT GTGTTTGAGA CAGGACCAGC AATGGCAAGT CGGCAAGTGG 
     4501 ATATTGCGAC CCAAGGCTGG TTCATAGGTC TAATGTGTGC TGTTGCCCTC CTCATCTTAA 
     4561 TTTTGCTAAT CGTTTGCTTC ATCAGAAGAA ACAAAGGTGG TAAATATCCA GTTAAAGAAA 
     4621 AGGAGGATGC TCACGCAGAC CCTGAAATCC AGCCCATGAA GGAGGATGAC GGAACGTTTG 
     4681 GAGAATATAG TGATGCAGAA GATCACAAGC CTTTGAAAAA AGGAAGTCGA ACACCTTCAG 
     4741 ACAGGACTGT GAAAAAAGAA GATAGTGATG ACAGCCTGGT TGACTATGGC GAGGGGGTGA 
     4801 ATGGCCAATT CAATGAGGAT GGCTCCTTTA TCGGACAATA CAGTGGTAAG AAAGAGAAAG 
     4861 AACCAGCAGA GGGAAATGAA AGCTCAGAGG CCCCTTCTCC TGTCAACGCA ATGAACTCCT 
     4921 TTGTTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4981 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5041 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5101 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5161 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5221 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5281 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5341 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5401 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5461 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5521 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5581 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5641 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5701 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5761 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5821 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5881 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5941 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6001 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6061 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6121 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6181 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6241 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6301 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6361 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6421 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6481 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6541 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6601 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6661 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6721 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6781 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6841 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6901 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6961 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7021 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7081 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7141 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7201 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7261 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7321 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7381 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7441 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7501 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7561 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7621 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7681 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7741 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7801 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7861 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7921 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7981 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8041 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8101 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8161 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8221 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8281 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8341 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8401 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8461 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8521 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8581 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8641 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8701 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8761 AAATTTAACG CGAATTTT
//