LOCUS       RR211820                7581 bp    DNA     circular  SYN 
DEFINITION  Dsc3 (Myc-DDK-tagged ORF) - Rat desmocollin 3 (Dsc3), (10 ug)
ACCESSION   NM_001107402
VERSION     NM_001107402.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3738..3829 
                     /label="Myc-DDK"  
     misc_feature    3859..4444 
                     /label="PolyA signal"  
     misc_feature    3916..3935 
                     /label="Restriction Enzyme"  
     misc_feature    4720..5392 
                     /label="ColE1"  
     misc_feature    5822..6616 
                     /label="Kan/Neo"  
     misc_feature    6651..6953 
                     /label="SV40 ori"  
     misc_feature    7039..7516 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3720..3726 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3720 
                     /label="Dsc3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGATGGCG ATGGCGGTCC CTGAGCTCCG GAGCCTGCCG TACAGGGCAC 
     1081 CATTCACGAA GTTCCTGCTG ACCCTCTGGG TCTTCAGTTT TGTTGGTGAA GCCTGTAAAG 
     1141 ATGTGACCTT TTATGTACCT TCAACACTAG AGGCAGACAA GATCATCGGA AGAGTTAGTT 
     1201 TGAAGGAGTG TCTCAGCTCG GCTGATGGTA TCATGCCAAG CGATCCTGAC TTCAGAGTTC 
     1261 TAGATGACGG GTCAGTTTAT CCTACCAGAG CTGTTGTGTT GTCTGATGAG AAAAGATCAT 
     1321 TTACCATTCA GCTTTCTGAT TCAAAGATGC GGAAACAGAA AGAGATCACT GTAATCCTGG 
     1381 AGCGCCAGAA GGAGGTAATG ATGAAAAGAC ACACTAATGA AACTGTCCTC AGAAGAGCCA 
     1441 AGAGGAGATG GGCACCCATT CCGTGTTCAA TGCAAGAAAA TTCCTTGGGT CCCTTCCCAT 
     1501 TGTTTCTTCA ACAGGTTCAG TCTGATTCAG CTCAGAACTA CAATATCTTC TACTCAATAA 
     1561 GCGGGCGTGG AGTTGACCAA GAACCACTGA ATTTGTTTTT CATTGAGAGA GACACTGGGA 
     1621 ATCTTTATTG TACTCGGCCT GTGGATCGTG AAGAATATGA TGTTTTTGAT TTGATTGCCT 
     1681 ATGCATCCAC TGCAGATGGC TACTCAGCAG ATTTACCTCT CCCCCTGCCC ATCAAAGTAG 
     1741 AGGATGAAAA TGACAATTAC CCTCTTTTTA CAGAAGCAAT TTACAATTTT GAAGTTCCAG 
     1801 AAAGCAGTAG ACTTGGTACC GTGGTGGGAA CGGTGTGTGC TACGGACAAA GATGAACCCG 
     1861 ATACGATGCA CACGCGCCTC AAGTACAGCA TCTTGGAGCA GAGTCCGCCG TCCCCAGGGC 
     1921 TCTTTTCTGT GCACCCTGAC ACCGGCGTCA TCACCACTGT CTCTCACTAC ATGGACAGAG 
     1981 AGGTTGTAGA CAAGTACAAA CTGATCTTGA AAGTCCAGGA CATGAATGGC CAATTCTTTG 
     2041 GACTCATCAG CACATCAACT TGTATCATAA CAGTGCAAGA TTCCAATGAC AATGCTCCCA 
     2101 CATTCAGACA AAATGCTTAT GAAACTTCTG TGGAGGAAAA TACATATAAT GTTGAGATCT 
     2161 TGCGCATACC TGTAGATGAT AAGGACATGG TTAACAGTGC CAACTGGAAG GCAAATTTCA 
     2221 CCATCTTAAA AGGAAATGAA AACGGCTGGT TCAAAATCAC GACAGACCCA GCGACAAATG 
     2281 AGGGTGTTCT GTGTGTCGTG AAGCCACTGG ATTATGAAGA AAATCGTCAA GTGACCCTAG 
     2341 AGATCGGTGT GAACAACGAA GCTCCATTCA TTAGAGATGT TGCAAGCCGA ATACCCACCA 
     2401 TGAATCGGGC CATGGTTACA GTCCAAGTGA GGGATCAGAA TGAGGGGCCG GAATGCAAGC 
     2461 CCCGTGAGCA GATTGTACGG ATCAAAGAGA ACTCAGCAAT TGGGTCTAAG ATCAATGGCT 
     2521 ACAAGGCCTA TGACCCCGAA ACCAAGAATA GCAATGGTTT AAGGTACAAA AAATTGCAGG 
     2581 ATCCTAAAGA CTGGGTCAGC ATTGAAGAAG TTTCAGGATC ACTCACCATA TCAAAAATCC 
     2641 TGGACAGAGA AATCATGGCT CCAAGAAATG ACCTATACAA TATTACAGTC ATGGCAATTG 
     2701 ACCAAGAGGG TAGATCATGC ACAGGGACGC TTGCAGTGAA CATTGAGGAT ATGAATGACA 
     2761 ACGCACCAGA GATAATTCAA GATGACATTG TCATCTGCAA GCCAAGGATG GGTTATACAG 
     2821 ACATTTCAGC TGTGGATCCT GATGAACCTA TTCACGGCCC TCCCTTCCAG TTCGCCTTGG 
     2881 CAAGCTCTTC TCCAGAAGTC AACAGAATCT GGACCCTCAA CCAAGTTAAT GATACAGCTG 
     2941 CCCGCCTGTC CTATCAGAAA ACCGCGGATG TTCAGATATA CTCCGTTCCT GTAACTGTAA 
     3001 CAGACAGAGC AGGCCAATCT GCGACCAAGA TCTTGAGAGT TAACCTGTGC GATTGTACTC 
     3061 ACCCCAGCCA GTGTCTGCTC AGATCAAGAA GAGCGGGGAT CACCCTGGGG AAGTGGGCAA 
     3121 TCCTTGCCAT CTTGCTGGGC ATCGCTCTGC TATTCTCGGT ACTGCTAACT TTAGTATGTG 
     3181 GGGTTGTTAC TGCTCAAAAA GGAAAACATT TTCCTGAAGA TTTAGCACAG CAAAACTTAA 
     3241 TTATATCAAA TACCGAAGCA CCCGGGGACG ACAGAGTGTG CTCTGCCAAT GGATTCACCA 
     3301 CCCACACTGC CAACAACTCC AGCCAAGGCT TCTGTGGCAC AATGGGGTCA GGAATGAGGA 
     3361 ACGGTGGGCA GGAGACCATC GAGATGATGA AAGGCCACCA GACCTTGGAC TCCTGCCGTG 
     3421 TTGCTGGGCA TCACCACACC CTGGACTCCT GCAGGGGAGG ACACATGGAA ACCGACAACT 
     3481 GCAGATACAC GTACTCAGAG TGGCACAGCT TCACTCAGCC CCGACTCGGC GAAAAATTGC 
     3541 ATGTGTGTAA TCAGAACGAA GACCACATCC CATCTCAAGA CTATGTCCTC ACCTATAACT 
     3601 ACGAAGGCAG GGGATCTCCA GCCGGTTCTG TAGGTTGCTG CAGTGAAAAG CAGGAAGAAG 
     3661 AGGGACTCGA CTTTTTAAAC AATTTGGAGC CCAAATTTCT TACATTGGCA GAAACATGTA 
     3721 CAAAGAGAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3781 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3841 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3901 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3961 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4021 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4081 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4141 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4201 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4261 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4321 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4381 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4441 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4501 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4561 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4621 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4681 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4741 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4801 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4861 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4921 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4981 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5041 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5101 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5161 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5221 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5281 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5341 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5401 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5461 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5521 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5581 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5641 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5701 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5761 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5821 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5881 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5941 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6001 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6061 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6121 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6181 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6241 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6301 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6361 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6421 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6481 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6541 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6601 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6661 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6721 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6781 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6841 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6901 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6961 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7021 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7081 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7141 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7201 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7261 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7321 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7381 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7441 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7501 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7561 CAAAAATTTA ACGCGAATTT T
//