LOCUS       RR211782                8511 bp    DNA     circular  SYN 
DEFINITION  Dhx8 (Myc-DDK-tagged ORF) - Rat DEAH (Asp-Glu-Ala-His) box polypeptide 8 (Dhx8), (10 ug)
ACCESSION   NM_001047844
VERSION     NM_001047844.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4668..4759 
                     /label="Myc-DDK"  
     misc_feature    4789..5374 
                     /label="PolyA signal"  
     misc_feature    4846..4865 
                     /label="Restriction Enzyme"  
     misc_feature    5650..6322 
                     /label="ColE1"  
     misc_feature    6752..7546 
                     /label="Kan/Neo"  
     misc_feature    7581..7883 
                     /label="SV40 ori"  
     misc_feature    7969..8446 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4650..4656 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4650 
                     /label="Dhx8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGTGGCC GTGGCCACCG CCGGGGTCCT AATGGGCTCG GAACCTGGCC 
     1081 CGGCGGAAGA GCTCGCTAAA CTCGAGTACC TGTCTTTGGT GTCAAAGGTT TGCACGGAGC 
     1141 TGGACAATCA TTTGGGCATC AACGACAAGG ATCTGGCTGA ATTTGTCATC AGTCTTGCTG 
     1201 AGAAAAATAC TACCTTTGAT ACTTTTAAGG CTTCTCTGGT CAAAAATGGT GCAGAATTCA 
     1261 CAACCATGTT GGATGAAGAA GATGTGAAAG TTGCTGTGGA TGTTTTGAAA GAGCTGGAAG 
     1321 CTTTAATGCC CAGTGCAGCA GGCCAAGAGA AACAAAGAGA CACTGAGCAC AGGGACAGGA 
     1381 CAAAGAAGAA GAAGAGGAGT CGGAGCCGAG ATCGTGATCG TGATCGAGAC CGGGACAGGG 
     1441 ACCGAGACCG GGACAGGGAC CGTGACAAGG ACCGAGAACG AGATAGAGAT CGAGAACGAG 
     1501 ACAGAGAGCG AGATAGAGAT AGAGACCATA AGCGGAGACA CCGATCACGC TCTCGGTCAC 
     1561 ATTCTAGGAC CCGGGAGAGG GCTAAGGGGA AATCTAGATA CAGGTCTAGG AGCAGAAGTC 
     1621 AGAGTCCCTT CAAAGATCGG AAAGACCGGG AAAAGTATGG GGAGAGGAAT CTGGACAGAT 
     1681 GGCGGGATAA GCATGTGGAC CGCCCTCCTC CTGAAGAGCC TGCCATTGGA GACATTTACA 
     1741 ATGGCAAAGT GACCAGCATC ATGCAGTTTG GCTGCTTCGT GCAGCTGGAA GGCCTAAGGA 
     1801 AGCGGTGGGA AGGCCTGGTG CACATTTCTG AGCTTCGACG GGAAGGTCGA GTGGCCAATG 
     1861 TGGCTGATGT TGTGAGCAAA GGCCAGAGGG TTAAGGTCAA AGTTCTGTCC TTCACTGGGA 
     1921 CCAAGACCAG CCTGAGCATG AAGGATGTGG ATCAAGAAAC AGGAGAAGAT CTAAACCCAA 
     1981 ACAGACGCAG AAATCTGGTT GGGGAGACCA ACGAAGAGAC GTCGATGCGG AACCCAGACC 
     2041 GACCCACCCA CCTCTCCCTC GTCAGTGCTC CTGAAGTAGA GGACGACTCA CTGGAGCGCA 
     2101 AGCGGCTCAC CCGCATCTCT GACCCAGAGA AGTGGGAGAT CAAACAGATG ATTGCTGCCA 
     2161 ATGTGCTTTC CAAAGAAGAG TTTCCAGACT TCGATGAAGA GACCGGCATT CTCCCTAAGG 
     2221 TGGATGATGA AGAAGATGAG GATCTTGAAA TTGAACTTGT TGAAGAGGAG CCTCCGTTCC 
     2281 TGAGAGGGCA CACCAAGCAG AGCATGGACA TGAGCCCCAT CAAAATCGTT AAGAACCCGG 
     2341 ATGGCTCCCT CTCCCAAGCA GCCATGATGC AGAGTGCCCT GGCCAAAGAA AGGCGGGAGC 
     2401 TGAAGCAGGC CCAGCGGGAA GCAGAGATGG ATTCTATACC CATGGGGCTC AACAAACACT 
     2461 GGGTGGACCC CTTGCCTGAT GCGGAAGGCA GGCAGATCGC TGCCAACATG AGGGGGATTG 
     2521 GGATGATGCC CAATGACATT CCTGAGTGGA AGAAGCATGC CTTTGGGGGC AACAAAGCTT 
     2581 CTTACGGGAA GAAGACCCAG ATGTCAATCC TTGAGCAGAG AGAGAGCCTG CCTATCTACA 
     2641 AACTGAAGGA ACAGCTGGTG CAGGCTGTAC ATGACAATCA GATCCTAATT GTAATCGGAG 
     2701 AGACAGGATC TGGGAAGACA ACACAGATCA CCCAGTACCT GGCAGAGGCA GGCTATACTT 
     2761 CCAGGGGCAA GATTGGGTGC ACCCAGCCCA GAAGAGTGGC AGCTATGTCT GTGGCCAAAA 
     2821 GAGTATCAGA GGAATTTGGT TGTTGCCTTG GTCAAGAGGT AGGCTACACC ATTCGATTTG 
     2881 AGGATTGCAC CAGCCCAGAA ACAGTCATCA AGTACATGAC AGACGGCATG CTGCTCAGAG 
     2941 AGTGCCTGAT TGATCCCGAT CTCACTCAGT ACGCCATCAT CATGCTGGAC GAGGCACATG 
     3001 AGAGGACTAT TCACACAGAT GTGCTCTTTG GGTTGTTGAA AAAGACAGTT CAAAAACGAC 
     3061 AAGACATGAA GCTGATTGTC ACCTCAGCCA CCCTGGATGC AGTGAAGTTT TCTCAGTACT 
     3121 TCTATGAAGC ACCCATCTTC ACAATCCCTG GTCGAACCTA TCCAGTGGAG ATTCTGTACA 
     3181 CAAAGGAGCC CGAAACAGAC TACTTGGACG CCAGCCTGAT CACAGTCATG CAGATCCACC 
     3241 TGACAGAGCC GCCAGGTGAC ATCTTGGTTT TTTTGACTGG TCAGGAAGAG ATCGATACTG 
     3301 CTTGTGAGAT CCTGTATGAA AGAATGAAGT CACTGGGACC TGACGTCCCA GAGTTGATCA 
     3361 TCCTCCCAGT GTACTCTGCT CTTCCGAGTG AGATGCAGAC CCGAATCTTT GATCCAGCTC 
     3421 CTCCTGGCAG CAGAAAGGTG GTGATTGCCA CCAACATTGC AGAAACATCT CTGACCATCG 
     3481 ACGGCATCTA CTATGTGGTC GACCCTGGAT TTGTGAAGCA AAAAGTTTAC AATTCTAAAA 
     3541 CAGGGATTGA CCAGCTCGTG GTGACACCTA TTTCTCAGGC TCAAGCAAAG CAGAGAGCTG 
     3601 GCAGAGCTGG GAGAACCGGC CCAGGCAAGT GTTACAGGCT GTACACAGAG CGTGCCTACC 
     3661 GGGATGAAAT GCTGACCACC AATGTGCCAG AAATCCAGAG AACCAACCTG GCCAGCACAG 
     3721 TGCTGTCCCT CAAGGCCATG GGCATTAATG ACCTGCTGTC CTTTGACTTC ATGGATGCGC 
     3781 CACCGATGGA AACTCTGATC ACAGCCATGG AGCAACTGTA TACGCTGGGG GCCCTGGACG 
     3841 ATGAGGGCCT GCTCACTCGC CTTGGCCGCA GGATGGCAGA GTTCCCTCTG GAGCCAATGC 
     3901 TCTGCAAAAT GCTCATCATG TCTGTGCATC TGGGCTGCAG CGAAGAGATG CTAACTATTG 
     3961 TGTCCATGCT GTCTGTGCAG AACGTCTTCT ACCGACCCAA GGACAAGCAG GCCCTTGCAG 
     4021 ATCAGAAGAA GGCCAAATTC CACCAGACAG AAGGGGACCA TCTCACCCTG CTGGCCGTGT 
     4081 ACAATTCCTG GAAGAACAAC AAATTCTCCA ACCCATGGTG CTATGAGAAC TTCATCCAGG 
     4141 CTCGTTCCTT ACGCCGGGCC CAGGACATTC GAAAGCAGAT GTTAGGCATA ATGGACAGGC 
     4201 ACAAGCTGGA TGTGGTCTCC TGTGGTAAAT CCACAGTGCG AGTGCAGAAG GCCATCTGCA 
     4261 GTGGCTTCTT CCGGAATGCT GCCAAGAAGG ACCCCCAGGA GGGCTACCGG ACATTGATTG 
     4321 ACCAGCAGGT GGTCTATATC CATCCTTCCA GTGCTCTTTT CAACAGACAG CCCGAATGGG 
     4381 TGGTATACCA TGAACTGGTG CTGACCACAA AGGAGTACAT GCGTGAGGTC ACCACCATCG 
     4441 ACCCCCGGTG GCTTGTGGAG TTTGCTCCGG CTTTCTTCAA AGTCTCTGAC CCAACCAAGC 
     4501 TAAGCAAGCA GAAGAAGCAG CAGCGCCTGG AGCCTCTGTA CAATCGTGGA GAAGGGATGC 
     4561 GTGTCCTCTA TACTGAGCTC CGGTCCTGGA AAGCTCAGGA TTGCTGGACC AACTATGGAT 
     4621 GCACCTATAG ATATTCCTTC CGTTTGTTAC CTGAGAGCAC GCGTACGCGG CCGCTCGAGC 
     4681 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4741 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4801 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4861 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4921 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4981 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5041 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5101 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5161 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5221 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5281 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5341 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5401 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5461 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5521 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5581 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5641 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5701 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5761 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5821 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5881 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5941 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6001 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6061 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6121 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6181 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6241 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6301 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6361 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6421 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6481 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6541 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6601 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6661 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6721 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6781 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6841 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6901 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6961 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7021 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7081 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7141 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7201 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7261 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7321 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7381 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7441 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7501 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7561 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7621 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7681 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7741 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7801 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7861 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7921 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7981 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8041 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8101 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8161 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8221 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8281 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8341 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8401 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8461 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//