LOCUS       RR211513                7020 bp    DNA     circular  SYN 
DEFINITION  Capn1 (Myc-DDK-tagged ORF) - Rat calpain 1 (Capn1), (10 ug)
ACCESSION   NM_019152
VERSION     NM_019152.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3177..3268 
                     /label="Myc-DDK"  
     misc_feature    3298..3883 
                     /label="PolyA signal"  
     misc_feature    3355..3374 
                     /label="Restriction Enzyme"  
     misc_feature    4159..4831 
                     /label="ColE1"  
     misc_feature    5261..6055 
                     /label="Kan/Neo"  
     misc_feature    6090..6392 
                     /label="SV40 ori"  
     misc_feature    6478..6955 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3159..3165 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3159 
                     /label="Capn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGAGGAG TTAATCACAC CTGTGTACTG CACCGGGGTG TCAGCCCAAG 
     1081 TGCAGAAGCA ACGGGACAAG GAGTTGGGCT TGGGCCGCCA TGAAAATGCC ATCAAGTACC 
     1141 TGGGCCAGGA TTATGAAAAT CTTCGTGCAA GATGCCTGCA GAACGGGGTC CTCTTCCAAG 
     1201 ACGATGCCTT CCCTCCAGTT TCTCATAGCT TGGGCTTCAA GGAACTGGGT CCCAATTCCT 
     1261 CTAAAACCTA TGGCATCAAG TGGAAGCGTC CTACGGAACT GCTGTCAAAC CCCCAGTTCA 
     1321 TCGTGGATGG AGCCACCCGA ACGGACATCT GCCAGGGAGC ACTGGGGGAC TGTTGGCTCC 
     1381 TGGCTGCCAT TGCCTCGCTC ACTCTCAACG AGACTATCCT CCACCGAGTG GTTCCCTACG 
     1441 GCCAGAGCTT CCAGGAGGGC TATGCTGGCA TCTTTCATTT CCAGCTGTGG CAGTTTGGGG 
     1501 AGTGGGTAGA TGTGGTTGTG GATGATTTGC TACCCACCAA GGATGGAAAG CTGGTGTTCG 
     1561 TGCACTCTGC CCAGGGCAAC GAGTTCTGGA GCGCTCTCCT GGAGAAAGCC TATGCAAAAG 
     1621 TGAATGGCAG CTATGAGGCC CTTTCGGGAG GCTGCACCTC AGAGGCCTTC GAGGACTTTA 
     1681 CCGGTGGGGT CACTGAGTGG TACGATCTGC AGAAGGCACC CAGCGACCTC TACCAGATCA 
     1741 TTCTGAAGGC CCTGGAACGA GGCTCCTTGC TGGGCTGCTC CATTAATATC TCCGATATCC 
     1801 GTGATTTGGA GGCTATTACT TTTAAGAACC TGGTGAGGGG CCATGCATAC TCTGTGACGG 
     1861 ATGCCAAGCA GGTCACTTAC CAGGGCCAGC GGGTGAACCT AATCCGGATG CGGAACCCCT 
     1921 GGGGTGAAGT GGAGTGGAAA GGACCCTGGA GTGACAACTC CTATGAGTGG AACAAAGTGG 
     1981 ACCCCTATGA ACGTGAGCAG CTGAGGGTCA AGATGGAGGA CGGGGAGTTC TGGATGTCCT 
     2041 TCCGGGACTT CATACGTGAG TTCACCAAAC TGGAAATCTG CAACCTTACA CCTGACGCCC 
     2101 TTAAGAGCAG AACCCTCCGG AACTGGAATA CCACATTTTA CGAGGGCACC TGGCGCCGGG 
     2161 GAAGCACTGC TGGAGGCTGC AGGAACTACC CAGCTACCTT CTGGGTAAAC CCCCAGTTCA 
     2221 AGATCCGGCT GGAGGAGGTG GATGACGCAG ACGACTATGA CAGTCGAGAG TCCGGCTGCA 
     2281 GCTTCTTGTT GGCCCTCATG CAGAAGCATC GCCGCAGGGA GCGTCGCTTT GGCCGGGACA 
     2341 TGGAGACCAT TGGTTTTGCA GTGTACCAGG TCCCTCGGGA GTTGGCGGGT CAGCCTGTGC 
     2401 ACTTGAAGCG TGACTTCTTC CTGGCCAATG CTTCTCGGGC ACAATCAGAG CACTTTATCA 
     2461 ACCTTCGAGA GGTCAGTAAC CGCATCCGCC TGCCGCCGGG GGAGTATATA GTGGTGCCCT 
     2521 CCACCTTCGA GCCCAACAAA GAGGGTGACT TTCTGCTGCG CTTCTTTTCA GAGAAGAAGG 
     2581 CTGGGACCCA GGAACTAGAT GACCAGATCC AGGCCAACCT CCCCGATGAG AAAGTGCTCT 
     2641 CTGAAGAGGA GATCGATGAC AACTTCAAAA CCCTGTTCAG CAAGTTGGCA GGGGATGACA 
     2701 TGGAGATCAG TGTCAAGGAG TTACAGACCA TCTTGAACAG AATCATTAGC AAACACAAAG 
     2761 ACCTGCGCAC TAACGGCTTC AGCCTGGAGT CGTGCCGCAG CATGGTGAAC CTCATGGATC 
     2821 GAGACGGTAA TGGGAAACTG GGCCTGGTGG AGTTCAACAT CCTGTGGAAC CGCATCCGAA 
     2881 ATTACCTGAC CATCTTCCGA AAGTTTGACC TGGACAAGTC TGGCAGCATG AGTGCTTATG 
     2941 AGATGAGGAT GGCCATCGAG GCTGCAGGCT TCAAGCTTAA CAAGAAGCTG CATGAGCTCA 
     3001 TCATCACCCG CTACTCAGAG CCCGACCTGG CCGTGGACTT TGACAACTTT GTGTGCTGCC 
     3061 TTGTGCGCCT GGAGACCATG TTCCGGTTTT TCAAAATTCT GGACACAGAC CTGGATGGTG 
     3121 TTGTGACCTT TGATCTATTT AAGTGGTTAC AGCTGACTAT GTTTGCAACG CGTACGCGGC 
     3181 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3241 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3301 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3361 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3421 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3481 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3541 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3601 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3661 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3721 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     3781 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     3841 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     3901 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     3961 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4021 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4081 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4141 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4201 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4261 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4321 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4381 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4441 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4501 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4561 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4621 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4681 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4741 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     4801 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     4861 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     4921 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     4981 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5041 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5101 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5161 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5221 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5281 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5341 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5401 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5461 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5521 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5581 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5641 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5701 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     5761 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     5821 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     5881 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     5941 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6001 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6061 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6121 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6181 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6241 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6301 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6361 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6421 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6481 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6541 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6601 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6661 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6721 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     6781 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     6841 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     6901 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     6961 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//