LOCUS       RR211471                9183 bp    DNA     circular  SYN 
DEFINITION  Ptch1 (Myc-DDK-tagged ORF) - Rat patched homolog 1 (Drosophila) (Ptch1), (10 ug)
ACCESSION   NM_053566
VERSION     NM_053566.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5340..5431 
                     /label="Myc-DDK"  
     misc_feature    5461..6046 
                     /label="PolyA signal"  
     misc_feature    5518..5537 
                     /label="Restriction Enzyme"  
     misc_feature    6322..6994 
                     /label="ColE1"  
     misc_feature    7424..8218 
                     /label="Kan/Neo"  
     misc_feature    8253..8555 
                     /label="SV40 ori"  
     misc_feature    8641..9118 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5322..5328 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5322 
                     /label="Ptch1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCGGCT GGTAACGCCG CCGGGGCCCT GGGCAGGCAG GCCGGCGGCG 
     1081 GGAGGCGCAG ACGGACCGGG GGACCGCACC GCGCCGCACC GGACCGGGAC TATCTGCACC 
     1141 GGCCCAGCTA CTGCGACGCC GCCTTCGCTC TGGAGCAGAT TTCCAAGGGG AAGGCTACTG 
     1201 GCCGGAAAGC GCCGCTGTGG CTGAGAGCGA AGTTTCAAAG ACTCTTATTT AAACTGGGTT 
     1261 GTTACATTCA AAAGAACTGC GGCAAGTTTT TGGTTGTGGG TCTCCTCATA TTTGGGGCCT 
     1321 TCGCTGTGGG ATTAAAGGCA GCTAATCTCG AGACCAACGT GGAGGAGCTG TGGGTGGAAG 
     1381 TTGGTGGACG AGTGAGTCGA GAATTAAATT ATACCCGCCA GAAGATAGGA GAAGAGGCTA 
     1441 TGTTTAATCC TCAACTCATG ATTCAGACTC CAAAAGAAGA AGGCGCTAAT GTCCTGACCA 
     1501 CAGAGGCCCT ACTACAACAC CTGGACTCAG CACTCCAGGC CAGCCGTGTG CACGTCTACA 
     1561 TGTATAACAG GCAATGGAAG TTGGAACATT TATGCTACAA ATCAGGGGAA CTTATCACAG 
     1621 AGACAGGCTA CATGGATCAG ATAATAGAAT ACCTTTACCC TTGCTTAATC ATTACACCTT 
     1681 TGGACTGCTT CTGGGAAGGG GCAAAGCTAC AGTCCGGGAC AGCGTACCTC CTAGGTAAGC 
     1741 CTCCTTTACG GTGGACAAAC TTTGACCCCT TGGAATTCCT AGAAGAGTTA AAGAAAATAA 
     1801 ACTACCAAGT GGACAGTTGG GAGGAAATGC TGAATAAAGC CGAAGTTGGC CATGGGTACA 
     1861 TGGACCGGCC TTGCCTCAAT CCAGCCGACC CAGATTGCCC TGCCACAGCC CCCAACAAAA 
     1921 ATTCAACCAA ACCTCTTGAT GTGGCCCTTG TTTTGAACGG TGGATGTCAA GGTTTATCCA 
     1981 GGAAGTATAT GCATTGGCAG GAGGAGTTGA TTGTGGGTGG CACCGTCAAG AACGCCACTG 
     2041 GAAAGCTTGT CAGCGCTCAT GCCCTGCAAA CCATGTTCCA GTTAATGACT CCCAAGCAAA 
     2101 TGTATGAACA CTTCAGGGGC TACGACTATG TCTCTCACAT CAACTGGAAT GAGGATAGGG 
     2161 CTGCCGCCAT CCTGGAGGCA TGGCAGAGGA CTTACGTGGA GGTGGTTCAT CAAAGTGTTG 
     2221 CCCCAAACTC CACTCAAAAG GTGCTTTCCT TCACCACCAC GACCCTGGAC GACATCCTAA 
     2281 AATCCTTCTC TGATGTCAGT GTCATCCGAG TGGCCAGTGG CTACCTACTG ATGCTTGCCT 
     2341 ACGCCTGTTT AACCATGCTG CGCTGGGACT GCTCCAAGTC CCAGGGTGCC GTGGGGTTGG 
     2401 CTGGTGTCCT GTTGGTTGCA CTGTCAGTGG CTGCAGGATT GGGCCTCTGC TCCTTGATTG 
     2461 GCATTTCCTT TAATGCTGCA ACAACTCAGG TTTTGCCATT TCTTGCCCTT GGTGTTGGTG 
     2521 TGGACGACGT CTTTCTCCTG GCCCATGCAT TCAGTGAAAC GGGACAGAAT AAGAGGATTC 
     2581 CATTTGAGGA CAGGACTGGG GAGTGCCTCA AGCGCACGGG AGCCAGCGTG GCCCTCACCT 
     2641 CCATCAGCAA TGTCACTGCC TTCTTCATGG CTGCATTAAT CCCTATTCCT GCACTGCGAG 
     2701 CGTTCTCCCT CCAGGCTGCT GTGGTGGTGG TATTCAATTT TGCCATGGTT CTACTCATTT 
     2761 TCCCTGCAAT TCTCAGCATG GATTTATACA GACGTGAGGA CAGAAGATTG GATATTTTCT 
     2821 GCTGTTTCAC AAGCCCCTGT GTCAGCCGGG TGATTCAAGT TGAACCGCAG GCCTACACAG 
     2881 AGCCTCATAG TAACACCCGG TACAGCCCCC CACCCCCGTA CAGCAGCCAC AGCTTCGCCC 
     2941 ATGAAACCCA CATCACCATG CAGTCCACCG TTCAGCTCCG CACAGAATAT GACCCTCACA 
     3001 CACACGTCTA CTACACCACC GCCGAGCCGC GCTCTGAGAT CTCTGTACAG CCTGTCACCA 
     3061 TCACCCAGGA CACCCTCAGC TGTCAGAGCC CTGAGAGCAC CAGTTCCACC CGGGACCTGC 
     3121 TTTCTCAATT CTCTGACTCC AGCCTCCACT GCCTTGAGCC CCCCTGCACC AAGTGGACAC 
     3181 TTTCTTCGTT TGCAGAGAAG CACTACGCTC CTTTCCTCTT GAGACCCAAA GCCAAGGTTG 
     3241 TGGTAATCCT TCTTTTCCTG GGCTTGCTGG GCGTCAGCCT TTATGGGACC ACCCGAGTGA 
     3301 GAGACGGGCT GGACCTCACG GACATTGTCC CCCGGGAAAC CAGAGAATAT GACTTCATAG 
     3361 CTGCCCAGTT CAAGTACTTC TCTTTCTACA ACATGTATAT AGTCACCCAG AAAGCAGACT 
     3421 ACCCAAATAT CCAACACCTG CTTTACGACC TTCATAAGAG TTTCAGCAGT GTGAAGTATG 
     3481 TCATGCTGGA GGAGAACAAG CAACTTCCCC AAATGTGGCT GCATTACTTT AGAGACTGGC 
     3541 TCCAAGGACT TCAGGATGCA TTTGACAGTG ACTGGGAAAC CGGGAGGATC ATGCCAAACA 
     3601 ATTATAAAAA TGGATCTGAT GATGGGGTCC TCGCTTACAA ACTCCTGGTG CAGACTGGCA 
     3661 GCCGAGACAA GCCCATCGAC ATTAGTCAGT TGACTAAACA GCGTCTGGTG GACGCAGATG 
     3721 GCATCATTAA TCCGAGCGCT TTCTACATCT ACCTGACCGC TTGGGTCAGC AATGACCCTG 
     3781 TAGCTTACGC TGCCTCCCAG GCCAACATCC GGCCTCACAG ACCAGAGTGG GTGCACGACA 
     3841 AAGCTGACTA CATGCCAGAG ACCAGGCTGA GAATCCCAGC AGCAGAGCCC ATAGAGTACG 
     3901 CTCAGTTCCC TTTCTACCTC AACGGCCTAC GGGACACCTC AGACTTTGTG GAAGCCATAG 
     3961 AAAAAGTGAG AGTCATCTGT AACAACTACA CGAGTCTGGG GCTGTCCAGC TACCCCAATG 
     4021 GCTACCCCTT CCTGTTCTGG GAGCAGTACA TCAGCCTGCG TCACTGGCTG CTGCTAGCCA 
     4081 TCAGCGTGGT GCTGGCCTGC ACATTTCTAG TGTGCGCAGT CTTCCTCCTG AACCCCTGGA 
     4141 CGGCCGGGAT CATTGTCATG GTCCTGGCTT TGATGACCGT TGAGCTCTTC GGCATGATGG 
     4201 GCCTCATTGG GATCAAGCTG AGTGCTGTGC CGGTGGTCAT CCTGATTGCG TCTGTTGGCA 
     4261 TTGGAGTAGA ATTCACCGTC CACGTGGCTT TGGCCTTTCT GACAGCCATT GGGGACAAGA 
     4321 ACCACAGGGC TATGCTCGCC CTGGAGCACA TGTTCGCTCC TGTTCTGGAC GGTGCTGTGT 
     4381 CCACTCTGCT GGGTGTACTG ATGCTTGCGG GATCCGAATT TGACTTCATT GTCAGATACT 
     4441 TCTTTGCCGT CCTGGCCATC CTCACCGTCT TGGGAGTTCT CAACGGACTG GTTCTGCTGC 
     4501 CTGTACTCTT ATCCTTCTTC GGACCATGTC CTGAGGTGTC TCCAGCCAAC GGCCTAAACC 
     4561 GGCTGCCCAC TCCTTCGCCT GAGCCGCCCC CAAGCGTCGT CCGGTTTGCC GTGCCTCCTG 
     4621 GTCACACGAA CAATGGGTCT GATTCCTCCG ACTCCGAGTA CAGCTCTCAG ACCACGGTGT 
     4681 CTGGCATCAG TGAGGAGCTC AGGCACTATG AAGCACAGCA GGGCACCGGA GGTCCTGCCC 
     4741 ACCAAGTGAT TGTGGAAGCC ACAGAAAACC CCGTCTTTGC CCGGTCCACT GTGGTGCATC 
     4801 CTGACTCCAG ACATCAGCCT CCCTTGAACT CTCGGCAACA GCCCCACCTG GACTCTGGCT 
     4861 CCCTGTCCCC TGGACGGCAA GGCCAGCAGC CTCGAAGGGA TCCCCCCAGA GAAGGCTTGC 
     4921 GGCCACCCCC CTACAGACCG CGCAGAGACG CTTTTGAAAT TTCTACTGAA GGGCATTCTG 
     4981 GCCCTAGCAA TAGGGACCGC TCGGGCCCCC ATGGGGCCCG TTCTCACAAC CCTCGGAACC 
     5041 CAACGTCCAC TGCCATGGGC AGCTCTGTGC CCAGCTACTG CCAGCCTATC ACCACTGTGA 
     5101 CGGCTTCCGC TTCTGTAACT GTCGCTGTGC ACCCCCCACC TGGACCTGGG CGCAACCCCC 
     5161 GAGGGGGACC CTGTCCAGGC TACGAGAGCT ACCCCGAGAC TGATCACGGG GTGTTCGAGG 
     5221 ATCCCCATGT GCCTTTTCAT GTCAGGTGTG AGAGGAGGGA CTCGAAGGTG GAGGTTATAG 
     5281 AACTACAGGA CGTGGAGTGT GAGGAGAGGC CGTGGGGGAG CAGCTCCAAC ACGCGTACGC 
     5341 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5401 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5461 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5521 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5581 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5641 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5701 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5761 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5821 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5881 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5941 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     6001 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     6061 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6121 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6181 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6241 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6301 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6361 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6421 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6481 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6541 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6601 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6661 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6721 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6781 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6841 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6901 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6961 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     7021 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     7081 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7141 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7201 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7261 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7321 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7381 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7441 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7501 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7561 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7621 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7681 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7741 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7801 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7861 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7921 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7981 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     8041 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     8101 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8161 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8221 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8281 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8341 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8401 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8461 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8521 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8581 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8641 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8701 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8761 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8821 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8881 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8941 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     9001 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     9061 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9121 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9181 TTT
//