LOCUS       RR211463                8214 bp    DNA     circular  SYN 
DEFINITION  Amer1 (Myc-DDK-tagged ORF) - Rat family with sequence similarity 123B (Fam123b), (10 ug)
ACCESSION   NM_001109320
VERSION     NM_001109320.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4371..4462 
                     /label="Myc-DDK"  
     misc_feature    4492..5077 
                     /label="PolyA signal"  
     misc_feature    4549..4568 
                     /label="Restriction Enzyme"  
     misc_feature    5353..6025 
                     /label="ColE1"  
     misc_feature    6455..7249 
                     /label="Kan/Neo"  
     misc_feature    7284..7586 
                     /label="SV40 ori"  
     misc_feature    7672..8149 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4353..4359 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4353 
                     /label="Amer1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGATTCAA CAGGATGAAA CAGTTCAAAT CAAGAGGACA TCAACTTCCA 
     1081 GTGATGCCCA AGATCAGGGA GCTGAGAAGG GAGCCAAGAA TAAGACAGCT GAGACAACAG 
     1141 AAGGATCAAC ATCAGAGCCA CCCTTATCAG GCCCAGGTAG GCTGAAGAAA ACTGCCTTGA 
     1201 AACTCTTTGG TGGCAAGAAG GGAATCTGTA CTCTGCCTAG TTTCTTTGGG GGTGGACGAA 
     1261 GCAAAGGTTC TGGGAAAGGC AGCTCCAAGA AGGGTCTAAA CAAGAGCAAG ACCCACGATG 
     1321 GTCTGAGTGA ATCATCTCAC GGCCCTGAAG ATGTTGTCGT TGAAGAAACA GAGCTCTCTA 
     1381 CACCTTTGTC CAAGTCACCT TCTCAATTTC CTAGCTCCCA GAGTGCCCAT GGAGCTTTGG 
     1441 AGATAGGCTC TAGACATAAG ACATCTGTGA CTGAAGCTGT AGAGAAAGCT GGAGTTGAGA 
     1501 AGGTACCCTC TGTGCACAAG CCGAAGAAAG GCCTAAAAGG CTTTTTAAAC AGTATCCGCC 
     1561 GTCACCGAAA GAGCAAGGTT TCTGGGGCTG ACCAAAGTGG GCTAGGGGCC AAGGAACTTG 
     1621 AGGGGACAAG AACCATGTCT CAAGAGCATG TGAGTTCAAT CTCTCTGCCC AGCTCTGAGG 
     1681 AGATATTCCG AGATGCTATA AAGGAAAATA CCAAACCCCA GGATGCCCCT GGACCAAAAA 
     1741 TGTCTCCAGC AAGAGAACAT TTTTCACTAA CTACTGAGAA GACAGCTTGT AAAAATCCAG 
     1801 AAAAACTTAC AATGACCTGT GCCTCAGAGT TCATGCAGCC CAAGCCTATT CTTGAAGCTA 
     1861 GTAGTCTAGA AGAGCCCCAC ACTTCAGAAA CAGAAGGAAA AGTGGTAACA GGAGACGCAA 
     1921 ACCCACCCAA TGGCCCGGTT GGGGATCAGC TAAGCCTCTT GTTTGGGGAT GTTACATCCC 
     1981 TGAAAAGCTT TGACTCGCTG ACAGGTTGTG GTGACATTAT AGCAGAACAG GACATGGACA 
     2041 GTATGACAGA TAGCATGGCC TCTGGTGGCC AGAGGGCCAA CCGAGATGGG ACCAAGCGAA 
     2101 GTTCCTGCTT GGTGACCTAC CAAGGAGGTG GGGAGGAAAT GGCCTTGCCA GATGACGATG 
     2161 ACAATGAAGA GGAAGAAGAA GAGGATGAGG AGGTAGAATT AGAGGATGAG GAAGAGGAGG 
     2221 TCAAGGATGG GGAGGAAGAT GATGACTTAG AGTATCTGTG GGCAAGTGCC CAGATATACC 
     2281 CAAGATTTAA TATGAACCTG GGCTATCATA CTGCTATATC TCCCAGCCAC CAGGGCTACA 
     2341 TGCTTCTTGA CCCAGTTCAG TCTTATCCTA ACCTAGGCCT GGGAGAACTT TTGACACCTC 
     2401 AGAGTGACCA GCAAGAGTCT GCTCCCAATA GTGATGAAGG TTATTATGAC TCTACCACAC 
     2461 CAGGATTTGA GGATGATTCG GGTGAGGCCC TGGGGCTTGT CCATAGGGAT TGTCTACCCA 
     2521 GAGACAGCTA TAGTGGTGAT GCTCTTTATG AATTCTATGA GCCAGATGAC AGTCTTGAGC 
     2581 ACTCCCCACC TGGGGATGAC TGCCTTTATG ACCTCCGTGG ACGCAACTCA GAGATGTTGG 
     2641 ATCCTTTCTT GAACTTGGAG CCCTTTTCTT CTCGACCACC TGGGGCAATG GAAACAGAAG 
     2701 AAGAACGGCT GGTAACCATC CAGAAGCAGT TGCTGTACTG GGAACTTCGC CGGGAGCAGC 
     2761 GTGAGGCTCA AGAGGCATGT GCCAGAGAGG CTCATGCCAG AGAAGCTTAT TCTCGAGACA 
     2821 CCCACACTCG GGAATCTTAC GGCAAAAATA CCCGAGCCCG AGAAACCCAA GCCCTAGAGG 
     2881 TTCATTCTCA AGAGGGTCGA GTCCAAGAGA CCAAAGTACG GCAAGAGAAG CCTGCCTTGG 
     2941 AATATCAGAT GAGGCCATTA GGCCCCTCGG TGATGGGTCT GGTGCCAGGG ACACCAGGAG 
     3001 GTTCTCAGAC TTCACACCGG GGAACCACTT CAGCTTTCCC AGCTACTTCC AGTAGTGAAC 
     3061 CAGACTGGCG GGATTTCCGT CCTCTGGAGA AGCGTTTTGA GGGGACTTGC TCCAAAAAAG 
     3121 ATCAAAGTAC TTGTTTAATG CAGCTCTTTC AGAGTGATGC CATGTTTGAA CCAGACATGC 
     3181 AAGAAGCAAA TTTCGGAGGC TCTCCCAGGA AGGCCTACCC TAGTTACTCA CCCCCTGAAG 
     3241 AGCCAGAGGA AGAGGAAGAT GAGAACGAAG GGAATGCCAC TGTGAGTTTT TCACAAGCTC 
     3301 TAGTAGAGTT TACCAGCAAT GGAAACCTTT TTACCAGCAT GTCCTACAGC TCTGATTCTG 
     3361 ACTCATCCTT CACTCAAAAT CTCCCTGAGT TGCCCCCCAT GGTCACCTTT GACATTGCTG 
     3421 ATGTGGAACG GGATGGAGAA GGCAAGTGTG AAGAGAATCC TGAGTTCAAC AATGATGAAG 
     3481 ATCTTACGGC CTCCTTGGAA GCTTTTGAGC TGGGTTATTA CCACAAACAT GCCTTCAACA 
     3541 GCTATCACAG CCGGTTCTAC CAAGGCCTGC CCTGGGGTGT TAGCAGCCTC CCTCGATACT 
     3601 TAGGACTCCC GGGTGTACAT CCTCGACCTC CACCCGCTGC AATGGCACTC AATAGGAGAA 
     3661 GCCGTTCCCT TGACAATGCA GAGACCCTGG AGCTGGAGCT CTCCAGTTCT CACCTAGCCC 
     3721 AGGGTTATGT GGAGTCTGAT GAGCTACAGG ACCACCAGGA AGATTCAGAT GAAGAAGGTG 
     3781 AAGAAGAGGA AGGAGAATGG GGCCGGGACA GCCCCTTGTC ACTCTACACT GAACCTCCAG 
     3841 GGGTCTATGA CTGGCCACCC TGGGCTCACT GTCCTGTTCC AGTGGGACCA GGTCTTGCTT 
     3901 GGCTAAGCCC TAATCAGTTG GATGAGCCTT TCAATCAGTC TTCATATGTG CAAGCAACCT 
     3961 GTTGTGTACC TCCTGTGGCA ATGTCAGTGC CAGGAAGAGC ACCTGGAGAC TCTGTGTCTC 
     4021 AGCCAGCTCG ACCTTCACAC CTACCCCTGC CCATGGGTCC TTGCTATAAC CTTCAATCAC 
     4081 AGGCCTCCCA GAGTGGGAGG GCCAGGCCTC GGGATGTACT GCTGCCTGTA GATGAGCCCA 
     4141 GTTGCTCCTC CATTTCTGGA GCCAACTCTC TGTCTCAAGC CAAGCCTGTA GGCATCACCC 
     4201 ATGGTATCCC TCAGCTGCCC AGGGTACGGC CTGAACCCCT TCAGCTTCAA CCCAATCACT 
     4261 ACCGAGCTTC CAACCTTGAC CTTTCAAAGG AGAGGGGTGA ACAAGGTGCC TCTCTCTCTA 
     4321 CCAGCTACTC CTCCACTGCC ATGAATGGAA ACCTAGCCAA GACGCGTACG CGGCCGCTCG 
     4381 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4441 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4501 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4561 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4621 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4681 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4741 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4801 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4861 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4921 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4981 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5041 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5101 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5161 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5221 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5281 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5341 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5401 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5461 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5521 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5581 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5641 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5701 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5761 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5821 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5881 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5941 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6001 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6061 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6121 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6181 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6241 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6301 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6361 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6421 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6481 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6541 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6601 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6661 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6721 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6781 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6841 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6901 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6961 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7021 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7081 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7141 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7201 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7261 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7321 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7381 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7441 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7501 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7621 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7681 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7741 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7801 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7861 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7921 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7981 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8041 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8101 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8161 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//