LOCUS       RR211431                7989 bp    DNA     circular  SYN 
DEFINITION  Llgl1 (Myc-DDK-tagged ORF) - Rat lethal giant larvae homolog 1 (Drosophila) (Llgl1), (10 ug)
ACCESSION   NM_152844
VERSION     NM_152844.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4146..4237 
                     /label="Myc-DDK"  
     misc_feature    4267..4852 
                     /label="PolyA signal"  
     misc_feature    4324..4343 
                     /label="Restriction Enzyme"  
     misc_feature    5128..5800 
                     /label="ColE1"  
     misc_feature    6230..7024 
                     /label="Kan/Neo"  
     misc_feature    7059..7361 
                     /label="SV40 ori"  
     misc_feature    7447..7924 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4128..4134 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4128 
                     /label="Llgl1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGAAGTTT CGGTTCCGGC GGCAGGGCGC AGACCCGCAG CGCGAGAAGC 
     1081 TCAAGCAGGA GCTCTTCACC TTCCACAAGA CTGTGGAGCA TGGCTTCCCC AACCAGCCCA 
     1141 GCGCCTTGGC CTTCGACCCG GAGCTTCGCA TCATGGCCAT CGGCACCAGA TCTGGGGCTG 
     1201 TCAAGATCTA TGGGGCACCC GGAGTGGAAT TTACAGGCCT ACACCGGGAT GCGGCCACTG 
     1261 TCACCCAGAT GCATTTCCTG CCTGGTCAGG GCCGACTTCT GACCCTGTTA GACGACAGCA 
     1321 GCTTACATCT GTGGGAAATC ATCCAGCGTA ATGGCTGTGC CCATCTGGAG GAAGGGCTCA 
     1381 GCTTCCACCC ACCCAGCCGG CCCAGTTTTG GCAATGCCAG TTTCCCTGCC GGTCTAACCC 
     1441 GAGTCACTGT GGTCCTGCTG GCAGCTGGCG ATACCGTGGT TCTGGGGACC GAGAGTGGTA 
     1501 GCATATTCTT CCTGGATGTC GCCACCCTGG CACTGCTGGA GGGGCAGACT CTCAGCCCAG 
     1561 ATGAGGTCCT GCGCAGCGTG CCAGATGACT ACCGGTGTGG AAAGGCCTTG GGCCCTGTGG 
     1621 AGTCACTCCA GGGACATCTG CAAGACCCCA GCAAGATCCT TATAGGCTAT AGTCGGGGCT 
     1681 TACTGGTCAT CTGGAGCCAG GCCACACAGT CTGTGGAGCA CGTTTTCCTG GGTAACCAGC 
     1741 AGCTGGAGAG CCTGTGTTGG GGGCGTGGTG GCAGCAACAT TATCAGCTCA CATAGTGATG 
     1801 GCAGCTATGC CATCTGGTCC ACAGACACTG GCAGCCCCCC AACGCTTCAG CCCACTGTAG 
     1861 TGACCACACC TTATGGCCCC TTCCCCTGCA AGGCCATCAA CAAGATTCTG TGGCGGAGCT 
     1921 GTGAGTCAGG AGACCACTTT ATCATCTTCA GTGGTGGCAT GCCTCGAGCC AGCTATGGTG 
     1981 ACCGCCACTG TGTGAGTGTA CTGCGGGCAG AGACACTGGT GACCCTAGAC TTCACCTCCC 
     2041 GTGTCATTGA CTTCTTCACG GTGCACAGCA CACAGCCAGA GGATGGTTTT GACAACCCCC 
     2101 AGGCCCTAGC CGTGCTTCTG GAGGAAGAGC TGGTGGTGCT GGACCTGCAG ACACCAGGCT 
     2161 GGCCAGCTGT GCCTGCCCCT TACCTGGCTC CACTGCATTC ATCAGCGATC ACCTGCTCTG 
     2221 CCCATGTTGC CAATGTGCCC AGCAAGCTGT GGGCCCGCAT CGTAAGTGCT GGCGAGCGGC 
     2281 AGAGCCCACA GCCTGCCTCC AGTGCCTTGA GTTGGCCCAT TACTGGGGGC CGGAACTTGG 
     2341 CCCAGGAACC CTCGCAGCGT GGGCTACTGC TGACCGGCCA TGAGGATGGC ACTGTGCGGT 
     2401 TCTGGGACGC CTCTGGTGTG GCGCTAAGGC CACTCTACAA ACTGAGCACA GCTGGCCTCT 
     2461 TTCAGACGGA CTGCGAACAT GCTGACAGCC TGGCTCAGGC TGTGGAGGAT GACTGGCCGC 
     2521 CCTTCCGCAA GGTGGGCTGC TTTGATCCCT ACAGTGATGA TCCCCGGCTA GGAATCCAGA 
     2581 AGGTTGCGCT TTGCAAGTAC ACAGCCCAGA TGGTGGTGGC TGGCACAGCA GGCCAGGTGC 
     2641 TGGTGCTGGA GCTCAGTGAC GTGCCGGGAG AGCACACCGT CAGTGTGGCC AGTGTGGATC 
     2701 TTCTCCAGGA TCGGGAGGGC TTCACGTGGA AGGGCCACGA GCGACTGAGC CCGCACACGG 
     2761 GACCGTTGCC TTGGCCTGCC GGGTTCCAGC CTCGCGTGCT GATACAGTGC CTGCCGCCCG 
     2821 CTGCTGTTAC TGCTGTTGCA CTCCATGCTG AGTGGAGCCT CGTGGCCTTT GGGACCAGTC 
     2881 ATGGCTTTGG CCTTTTTGAC TACCAGCGCA AGAGCCCTGT GCTGGCCAGG TGTACCCTTC 
     2941 ACCCCAATGA CTCTCTGGCC ATGGAGGGGC CACTGTCCCG GGTGAAGTCC CTCAAGAAGT 
     3001 CACTGCGACA GTCATTCCGG CGTATCCGCA AGAGCCGTGT CTCAGGCAAA AAACGGGCTA 
     3061 CCACTGCCAG CAGCAAGTTG CAGGAGGCCA ATGCACAGCT GGCAGAGCAG ACATGCCCAC 
     3121 ACGACGTGGA GATGACACCT GTGCAGCGCC GCATCGAGCC TCGCTCTGCT GATGACTCCC 
     3181 TTTCTGGTGT TGTACGCTGC CTCTACTTTG CTGACACATT CCTTCGAGAT GCGACCCACC 
     3241 ATGGGCCCAC CATGTGGGCA GGCACCAACT CGGGCTCTGT ATTTGCCTAT GCGCTGGAGG 
     3301 TTCCAGCAGC CACGGCAGGT GGTGAAAAGC GGCCTGAGCA GGCAGTGGAG GCTGTGCTGG 
     3361 GCAAGGAGGT GCAACTAATG CATCGGGCAC CCGTGGTGGC CATTGCTGTG TTGGATGGCC 
     3421 GTGGCCGTCC GCTGCCTGAG CCGTATGAGG CTTCCCGGGA CCTGGCCCAG GCGCCAGACA 
     3481 TGCAAGGCGG CCATGCTGTG CTCATTGCAT CTGAGGAACA ATTCAAGGTG TTCACACTAC 
     3541 CCAAGGTGAG TGCTAAGACT AAATTCAAGC TTACAGCCCA TGAAGGCTGT CGTGTGCGGA 
     3601 AGGTAGCCCT GGCTACATTT GCCAGCGTGA TGTCTGAGGA CTATGCCGAG ACCTGCCTTG 
     3661 CCTGCCTCAC CAACCTGGGT GATGTCCACG TCTTCTCGGT GCCTGGCCTG AGGCCTCAGG 
     3721 TGCACTACTC CTGTATCCGG AAGGAGGACA TCAGTGGCAT CGCTTCCTGT GTCTTCACAC 
     3781 GTCACGGCCA GGGCTTTTAC TTGATTTCTC CATCGGAATT TGAGCGCTTC TCACTGAGTG 
     3841 CTCGCAACAT CACAGAACCA CTATGTTCTC TGGATATAAG CTGGCCCCAA AATGCCACCC 
     3901 AGCCCAGGCT TCAAGAGTCA CCCAAGCTGA GCCAGGCTAA TGGGACCAGA GACATCATTC 
     3961 TGGCCCCAGA GAGCTGCGAA GGAAGCCCTA GCTCTGCCCA CAGCAAGCGA GCTGATACCA 
     4021 TGGAACCACC CGAGGCCGCT CTCTCGCCTG TGTCCATTGA TTCAGCTGCT AGTGGGGATA 
     4081 CCATGCTGGA CACAACAGGG GATGTCACCG TGGAATATGT GAAGGATTTT CTGGGGACGC 
     4141 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4201 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4261 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4321 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4381 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4441 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4501 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4561 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4621 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4681 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4741 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4801 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4861 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4921 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4981 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5041 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5101 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5161 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5221 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5281 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5341 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5401 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5461 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5521 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5581 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5641 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5701 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5761 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5821 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5881 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5941 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6001 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6061 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6121 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6181 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6241 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6301 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6361 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6421 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6481 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6541 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6601 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6661 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6721 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6781 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6841 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6901 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6961 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7021 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7081 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7141 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7201 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7261 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7321 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7381 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7441 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7501 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7561 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7621 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7681 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7741 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7801 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7861 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7921 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7981 GCGAATTTT
//