LOCUS       RR211374                8586 bp    DNA     circular  SYN 
DEFINITION  Cfh (Myc-DDK-tagged ORF) - Rat complement factor H (Cfh), (10 ug)
ACCESSION   NM_130409
VERSION     NM_130409.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4743..4834 
                     /label="Myc-DDK"  
     misc_feature    4864..5449 
                     /label="PolyA signal"  
     misc_feature    4921..4940 
                     /label="Restriction Enzyme"  
     misc_feature    5725..6397 
                     /label="ColE1"  
     misc_feature    6827..7621 
                     /label="Kan/Neo"  
     misc_feature    7656..7958 
                     /label="SV40 ori"  
     misc_feature    8044..8521 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4725..4731 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4725 
                     /label="Cfh"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGACTGTCA GCAAGAATTA TTTGGCTTAT ATTATGGACT GTTTGTGTAG 
     1081 CAGAAGATTG TAAAGGTCCT CCTCCAAGAG AAAATTCAGA AATTCTCTCA GGTTCGTGGT 
     1141 CTGAACAACT ATATTCAGAA GGCACTCAGG CAACCTACAA ATGCCGCCCT GGATACCGAA 
     1201 CACTTGGTAC TATTGTAAAA GTATGCAAGA ATGGAGAATG GGTACCTTCT AACCCATCAA 
     1261 GGATATGTCG GAAAAGGCCA TGTGGGCATC CCGGAGACAC ACCCTTTGGG TCCTTTAGGC 
     1321 TGGCAGTTGG ATCTGAATTT GAATTTGGTG CAAAGGTTGT TTATACATGT GATGAAGGGT 
     1381 ACCAACTCTT AGGTGAAATT GATTACCGTG AATGTGATGC AGATGGGTGG ACCAATGATA 
     1441 TTCCAATATG TGAAGTTGTG AAGTGCTTGC CAGTGACAGA ACTGGAGAAT GGAAGAATTG 
     1501 TGAGTGGTGC AGCCGAACCA GACCAGGAAT ATTATTTTGG ACAGGTGGTA CGCTTTGAAT 
     1561 GCAACTCCGG CTTCAAGATT GAAGGACAGA AAGAAATGCA CTGCTCAGAA AATGGCCTCT 
     1621 GGAGCAATGA AAAGCCACAG TGTGTGGAAA TTTCTTGCCT GCCACCACGA GTTGAAAATG 
     1681 GAGATGGTAT ATATCTGAAA CCAGTTTACA AGGAGAATGA AAGATTCCAA TATAAATGTA 
     1741 AGCAAGGTTT TGTGTACAAA GAAAGAGGGG ATGCTGTCTG CACGGGTTCT GGATGGAATC 
     1801 CTCAGCCTTC CTGTGAAGAA ATGACATGTT TGACTCCATA TATTCCAAAT GGTATCTACA 
     1861 CACCTCACAG GATTAAACAC AGAATTGATG ATGAAATCAG ATATGAATGT AAAAATGGCT 
     1921 TCTATCCTGC AACCCGATCA CCTGTTTCAA AGTGTACAAT TACTGGCTGG ATCCCTGCTC 
     1981 CAAGATGTAG CTTGAAACCT TGTGATTTTC CACAATTCAA ACATGGACGT CTGTATTATG 
     2041 AAGAAAGCCG GAGACCCTAC TTCCCAGTAC CTATAGGAAA GGAGTACAGC TATTACTGTG 
     2101 ACAACGGGTT TACAACGCCT TCACAGTCAT ACTGGGACTA CCTTCGTTGC ACAGTAAATG 
     2161 GGTGGGAGCC TGAAGTTCCA TGCCTCAGGC AATGTATTTT CCATTATGTG GAATATGGAG 
     2221 AATCTTTATA CTGGCAAAGA AGATATATAG AGGGTCAGTC TGCAAAAGTC CAGTGTCACA 
     2281 GTGGCTATAG TCTTCCAAAT GGTCAAGATA CAATATTATG TACAGAAAAT GGCTGGTCCC 
     2341 CTCCTCCCAA ATGCGTCCGT ATCAAGACTT GTTCAGTATC AGATATAGAA ATTGAAAATG 
     2401 GGTTTTTTTC TGAATCTGAT TATACATATG CTCTAAATAG AAAAACACGG TATAGATGTA 
     2461 AACAGGGATA TGTAACAAAT ACCGGAGAAA TATCAGGAAT AATTACTTGT CTTCAAGATG 
     2521 GATGGTCACC TCGACCCTCA TGCATTAAGT CTTGTGATAT GCCTGTATTT GAGAATGCTA 
     2581 TGACTAAGAA TAATAACACA TGGTTTAAAC TCAATGACAA ATTAGACTAT GAATGTCACA 
     2641 TTGGATATGA AAATGAATAT AAACATACCA AAGGCTCTAT AACATGTACT TATGATGGAT 
     2701 GGTCTAGTAC ACCCTCCTGT TATGAAAGAG AATGCAGCAT TCCCCTGTTA CACCAAGACT 
     2761 TAGTTGTTTT TCCCAGAGAA GTAAAATACA AAGTTGGAGA TTCGTTGAGT TTCTCTTGCC 
     2821 GTTCAGGACA CAGAGTTGGA GCAGATTTAG TGCAATGCTA CCACTTTGGA TGGTCCCCTA 
     2881 ATTTCCCAAC GTGTGAAGGC CAAGTAAAAT CATGTGACCA ACCTCTTGAA ATCCCGAATG 
     2941 GGGAAATAAA GGGAACAAAA AAAGTTGAAT ACAGCCATGG TGACGTGGTG GAATATGATT 
     3001 GCAAACCTAG ATTTCTACTG AAGGGACCCA ATAAAATCCA GTGTGTTGAC GGGAAGTGGA 
     3061 CAACCTTGCC GATATGCGTT GAGTATGAGA GAACATGTGG AGACCTTCCT GCACTTGAGC 
     3121 ATGGCTCTGT CCAGTTATCT GTCCCTCCCT ACCACCACGG AGATTCAGTG GAGTTCACTT 
     3181 GTGCAGAAAC CTTCACAATG ATTGGGCATG CAGTAGTTTT CTGCATTAGT GGAAGGTGGA 
     3241 CCGAGCTTCC TCAATGTGTT GCAACAGATC AACTGGAGAA GTGTAAAGCC CCGAAGTCAA 
     3301 CTGGCATAGA TGCAATTCAT CCAAATAAGA ATGAATTTAA TCATAACTTT AGTGTGAGTT 
     3361 ACAGATGTAG ACAAAAGCAG GAGTATGAAC ATTCAATCTG CATCAATGGA AGATGGGATC 
     3421 CTGAACCAAA CTGTACAAGA AATGAGAAAA GATTCTGCCC TCCTCCCCCA CAGATTCCAA 
     3481 ATGCCCAAGT GATTGAAACC ACAGTGAAAT ACTTGGATGG AGAGAAAGTA TCTGTTCTTT 
     3541 GCCAAGATGG TTACCTAACT CAGGGCCCAG AAGAAATGGT GTGTAAACAT GGAAGGTGGC 
     3601 AGTCGTTACC ACGCTGCACG GAAAAAATTC CATGTTCCCA GCCCCCTAAA ATTGAACATG 
     3661 GATCTATTAA GTCGCCCAGG TCCTCAGAAG AGAGAGATTT AATTGAGTCC AGCAGTTATG 
     3721 AACACGGAAC TACATTCAGC TATGTCTGTG ATGATGGATT CAGGATATCT GAAGAAAATA 
     3781 GGGTAACCTG CAACATGGGA AAATGGAGCT CTCTGCCTCG TTGTGTTGGA ATACCTTGTG 
     3841 GACCCCCACC TTCAATTCCT CTTGGTATTG TTTCTCATGA ACTAGAAAGT TACCAATATG 
     3901 GAGAGGAGGT TACATACAAT TGTTCTGAAG GCTTTGGAAT TGATGGACCA GCATTTATTA 
     3961 AATGTGTAGG AGGACAGTGG TCTGAACCAC CCAAATGCAT AAAAACTGAT TGTGACAACT 
     4021 TGCCCACATT TGAAATTGCC AAACCGACAG AAAAGAAAAA AAAATCATAC AGGTCAGGAG 
     4081 AACAAGTGAC ATTCAGATGT CCACCTCCGT ATCGAATGGA TGGCTCTGAC ATTGTCACAT 
     4141 GTGTTAATAC GAAGTGGATT GGACAGCCGG TATGCAAAGA TAATTCCTGT GTGAATCCAC 
     4201 CACATGTGCC AAATGCTACT ATACTAACAA GGCACAAGAC TAAATATCCA TCTGGTGACA 
     4261 AAGTACGTTA TGACTGTAAT AAACCTTTTG AATTATTTGG GGAAGTGGAA GTGATGTGCC 
     4321 AAAACGGGAT TTGGACAGAA CCACCGAAAT GCAAAGATTC AACAGGGAAA TGTGGGCCTC 
     4381 CTCCACCTAT TGACAATGGA GACATCACCT CCTTGTCATT ACCAGTATAT GCACCATTAT 
     4441 CATCAGTTGA ATATCAATGC CAGAACTATT ATCTACTTAA GGGAAATAAG ATAGTAACAT 
     4501 GTAGAAATGG AAAGTGGTCT CAGCCACCAA CCTGCTTACA TGCATGTGTG ATACCAGAAG 
     4561 ATATTATGGA AAAACATAAT ATAGTTCTCA GATGGAGGGA AAATGCAAAG ATTTATTCCC 
     4621 AATCAGGGGA GAATATTGAA TTCATGTGTA AACCTGGATA TAGAAAATTC AGAGGATCAC 
     4681 CTCCGTTTCG TACAAAGTGC ATTGAGGGTC ACATCAATTA TCCCACTTGT GTAACGCGTA 
     4741 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4801 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4861 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4921 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4981 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5041 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5101 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5161 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5221 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5281 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5341 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5401 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5461 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5521 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5581 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5641 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5701 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5761 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5821 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5881 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5941 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6001 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6061 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6121 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6181 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6241 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6301 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6361 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6421 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6481 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6541 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6601 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6661 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6721 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6781 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6841 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6901 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6961 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7021 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7081 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7141 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7201 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7261 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7321 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7381 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7441 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7501 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7561 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7621 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7681 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7741 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7801 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8041 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8101 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8161 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8221 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8281 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8341 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8401 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8461 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8521 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8581 AATTTT
//