LOCUS       RR211342                8193 bp    DNA     circular  SYN 
DEFINITION  Trpm8 (Myc-DDK-tagged ORF) - Rat transient receptor potential cation channel, subfamily M, member 8 (Trpm8), (10 ug)
ACCESSION   NM_134371
VERSION     NM_134371.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4350..4441 
                     /label="Myc-DDK"  
     misc_feature    4471..5056 
                     /label="PolyA signal"  
     misc_feature    4528..4547 
                     /label="Restriction Enzyme"  
     misc_feature    5332..6004 
                     /label="ColE1"  
     misc_feature    6434..7228 
                     /label="Kan/Neo"  
     misc_feature    7263..7565 
                     /label="SV40 ori"  
     misc_feature    7651..8128 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4332..4338 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4332 
                     /label="Trpm8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTTCGAG GGAGCCAGGC TCAGCATGAG GAGCCGCAGA AATGGAACTC 
     1081 TGGGCAGCAC CCGGACCCTG TACTCCAGCG TGTCTCGGAG CACAGACGTG TCCTACAGTG 
     1141 AAAGTGATTT GGTGAATTTT ATTCAGGCAA ATTTTAAAAA ACGAGAATGC GTCTTCTTTA 
     1201 CCAGAGACTC CAAGGCCATG GAGAGCATAT GCAAGTGTGG TTATGCCCAG AGCCAGCATA 
     1261 TCGAAGGCAC CCAGATCAAC CAAAATGAGA AGTGGAACTA CAAAAAACAC ACCAAGGAGT 
     1321 TTCCAACAGA CGCCTTTGGG GACATTCAGT TTGAGACTCT GGGGAAGAAA GGCAAGTACT 
     1381 TACGCTTATC CTGTGACACG GACTCTGAAA CCCTCTACGA ACTGCTGACC CAGCACTGGC 
     1441 ACCTCAAAAC ACCCAACCTG GTCATCTCAG TGACGGGTGG AGCCAAAAAC TTTGCTTTGA 
     1501 AGCCACGCAT GCGCAAAATC TTCAGTCGGC TGATCTACAT CGCTCAGTCT AAAGGGGCAT 
     1561 GGATTCTTAC CGGAGGCACT CATTACGGTC TGATGAAGTA CATAGGTGAA GTGGTGAGGG 
     1621 ATAACACCAT CAGCAGGAAC TCGGAAGAGA ACATCGTGGC CATTGGCATA GCGGCCTGGG 
     1681 GCATGGTCTC CAACAGGGAC ACCCTCATCA GGAATTGTGA TGATGAGGGA CATTTTTCAG 
     1741 CTCAATATAT CATGGATGAC TTCATGAGAG ATCCTCTCTA CATCCTGGAC AACAATCATA 
     1801 CCCACCTGCT GCTTGTGGAC AACGGTTGTC ATGGACACCC CACGGTGGAA GCCAAACTTC 
     1861 GGAATCAGCT GGAGAAGTAC ATCTCTGAGC GCACCAGTCA AGATTCCAAC TATGGTGGTA 
     1921 AGATCCCCAT CGTGTGTTTT GCCCAGGGAG GTGGAAGAGA AACTTTGAAA GCCATCAACA 
     1981 CCTCTGTCAA AAGTAAGATC CCCTGTGTGG TGGTGGAAGG CTCGGGGCAG ATTGCCGATG 
     2041 TGATTGCCAG CCTGGTGGAG GTAGAGGATG TTTTAACCTC TTCCATGGTC AAAGAGAAGC 
     2101 TGGTACGGTT TTTACCCCGC ACTGTGTCCC GGCTGCCTGA AGAGGAGATT GAGAGCTGGA 
     2161 TCAAATGGCT CAAAGAAATT CTTGAGAGCC CCCACCTCCT CACGGTCATC AAGATGGAGG 
     2221 AGGCTGGAGA CGAGGTCGTG AGCAGCGCCA TTTCCTACGC GCTGTACAAA GCCTTCAGCA 
     2281 CTAATGAACA AGACAAGGAC AACTGGAACG GACAGCTGAA GCTTCTGCTG GAGTGGAACC 
     2341 AACTGGACCT TGCCAGTGAT GAGATCTTCA CCAATGACCG CCGCTGGGAG TCTGCCGACC 
     2401 TTCAGGAAGT CATGTTCACG GCCCTCATAA AGGACAGGCC CAAGTTTGTC CGCCTCTTCC 
     2461 TGGAGAATGG CCTCAACCTG CAGAAGTTCC TCACCAATGA AGTCCTCACG GAGCTCTTCT 
     2521 CCACCCACTT CAGCACCCTA GTGTACCGGA ACCTGCAGAT CGCCAAGAAC TCCTACAACG 
     2581 ATGCACTCCT TACCTTTGTC TGGAAGTTGG TGGCAAACTT CCGTAGAAGC TTCTGGAAAG 
     2641 AGGACAGAAG CAGCAGGGAG GACTTGGATG TGGAACTCCA TGATGCATCT CTCACCACCC 
     2701 GGCACCCCCT GCAGGCTCTT TTCATCTGGG CCATTCTTCA GAACAAGAAG GAACTCTCCA 
     2761 AGGTCATCTG GGAGCAAACC AAAGGCTGTA CTCTGGCCGC CTTGGGGGCC AGCAAACTTC 
     2821 TGAAGACCCT GGCCAAAGTT AAGAATGATA TCAACGCAGC TGGGGAATCT GAGGAACTGG 
     2881 CTAATGAGTA TGAGACCCGA GCAGTGGAGT TGTTCACTGA GTGTTACAGC AGTGATGAGG 
     2941 ACTTGGCAGA ACAGCTACTG GTCTACTCTT GTGAAGCCTG GGGTGGGAGC AACTGTCTGG 
     3001 AGCTGGCGGT GGAGGCTACG GACCAGCATT TCATTGCTCA GCCTGGGGTC CAGAATTTCC 
     3061 TTTCTAAGCA ATGGTATGGA GAGATTTCCC GAGACACGAA GAACTGGAAG ATTATCCTGT 
     3121 GTCTGTTCAT CATCCCCCTG GTGGGCTGTG GCCTCGTATC GTTTAGGAAG AAGCCCATTG 
     3181 ACAAGCACAA GAAGCTGCTC TGGTACTACG TGGCCTTCTT CACTTCGCCC TTCGTGGTCT 
     3241 TCTCCTGGAA CGTGGTCTTC TACATCGCCT TCCTCCTGCT GTTTGCGTAT GTGCTGCTCA 
     3301 TGGACTTCCA CTCGGTGCCA CACACCCCCG AGCTGATCCT CTATGCCCTG GTCTTCGTCC 
     3361 TCTTCTGTGA TGAAGTGAGG CAGTGGTACA TGAACGGAGT GAATTATTTC ACCGACCTAT 
     3421 GGAACGTTAT GGACACACTG GGACTTTTCT ACTTCATAGC GGGTATTGTA TTCCGGCTTC 
     3481 ACTCTTCAAA TAAAAGCTCT TTGTACTCCG GGCGAGTCAT TTTCTGTCTG GATTACATTA 
     3541 TATTCACTCT AAGGCTCATC CACATTTTCA CCGTGAGCAG GAACCTGGGA CCCAAGATTA 
     3601 TAATGCTGCA GCGGATGCTC ATCGACGTTT TCTTCTTCTT GTTTCTCTTT GCTGTGTGGA 
     3661 TGGTGGCCTT CGGCGTAGCC AGACAGGGGA TCCTTAGGCA AAATGAACAG CGCTGGAGGT 
     3721 GGATCTTCCG CTCTGTCATC TATGAGCCCT ACCTGGCCAT GTTTGGCCAG GTGCCCAGTG 
     3781 ATGTGGACAG TACCACATAT GACTTCTCCC ACTGCACCTT CTCGGGAAAT GAGTCCAAGC 
     3841 CACTGTGCGT GGAGCTAGAT GAATACAATC TGCCCCGCTT CCCTGAGTGG ATCACCATCC 
     3901 CACTAGTGTG CATCTACATG CTCTCCACCA ACATCCTTCT GGTCAATCTC CTGGTCGCCA 
     3961 TGTTTGGCTA CACGGTGGGC ATTGTGCAGG AGAACAACGA TCAGGTCTGG AAGTTCCAGC 
     4021 GGTACTTCCT GGTGCAGGAG TACTGCAACC GCCTCAACAT CCCCTTCCCC TTCGTCGTCT 
     4081 TCGCTTACTT CTACATGGTG GTCAAGAAGT GTTTCAAATG CTGCTGTAAA GAGAAGAACA 
     4141 CGGAGTCTTC TGCCTGCTGT TTCAGAAATG AGGACAACGA GACTTTGGCG TGGGAGGGCG 
     4201 TCATGAAGGA GAATTACCTT GTCAAGATCA ACACGAAGGC CAACGACAAC GCAGAGGAGA 
     4261 TGAGGCATCG GTTCAGACAA CTGGACACAA AGCTTAATGA TCTCAAAGGT CTTCTGAAAG 
     4321 AGATTGCTAA TAAAATCAAA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4381 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4441 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4501 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4561 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4621 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4681 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4741 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4801 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4861 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4921 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4981 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5041 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5101 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5161 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5221 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5281 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5341 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5461 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5521 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5581 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5641 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5761 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5821 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5881 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5941 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6001 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6061 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6121 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6181 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6241 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6301 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6361 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6421 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6481 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6541 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6601 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6661 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6721 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6781 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6841 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6901 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6961 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7021 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7081 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7141 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7201 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7261 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7321 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7381 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7441 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7501 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7561 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7621 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7681 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7741 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7801 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7861 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7921 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7981 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8041 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8101 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8161 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//