LOCUS       RR211332                6202 bp    DNA     circular  SYN 
DEFINITION  Wnt10a (Myc-DDK-tagged ORF) - Rat wingless-type MMTV integration site family, member 10A (Wnt10a), (10 ug)
ACCESSION   NM_001108227
VERSION     NM_001108227.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2357..2448 
                     /label="Myc-DDK"  
     misc_feature    2478..3063 
                     /label="PolyA signal"  
     misc_feature    2535..2554 
                     /label="Restriction Enzyme"  
     misc_feature    3339..4011 
                     /label="ColE1"  
     misc_feature    4441..5235 
                     /label="Kan/Neo"  
     misc_feature    5270..5572 
                     /label="SV40 ori"  
     misc_feature    5658..6135 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2332..2339 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2332 
                     /label="Wnt10a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAGCGCC CACCCTCGCC CCTGGCTGCG GCTCCCACCA GGGCCCCAGC 
     1081 CGCGGCCTGA GCTCTGGGCG CTCCTGTTCT TCCTACTGCT GCTGGCTGCT GCTGTGCCCA 
     1141 GGTCAGCACC CAACGACATC CTGGGCCTCC GCCTACCCCC AGAGCCTGTG CTCAACGCCA 
     1201 ACACAGTGTG CCTGACATTG CCCGGCCTGA GCCGGCGGCA GATGGAGGTG TGTGTGCGTC 
     1261 ACCCTGACGT GGCCGCCTCT GCTATCCAGG GCATCCAGAT CGCCATCCAT GAGTGCCAGC 
     1321 ATCAGTTCCG GGACCAGCGC TGGAACTGTT CCAGCCTGGA GACTCGGAAC AAAGTCCCCT 
     1381 ACGAGAGCCC CATCTTCAGC AGAGGTTTTC GAGAGAGTGC TTTCGCCTAC GCCATAGCAG 
     1441 CTGCCGGGGT GGTGCACGCA GTGTCCAACG CGTGCGCCCT GGGTAAACTG AAGGCTTGCG 
     1501 GTTGCGATGC CTCCAGGCGT GGGGACGAAG AGGCCTTTCG TCGGAAGCTG CACCGCTTGC 
     1561 AGCTGGACGC CCTACAGCGC GGAAAGGGCT TGAGCCACGG GGTCCCGGAG CACCCTGCCA 
     1621 TACCCCCTGC CAGCCCAGGC CTGCAGGACT CCTGGGAGTG GGGTGGCTGC AGTCCGGATG 
     1681 TGGGCTTTGG AGAACGCTTC TCCAAGGACT TTCTGGACTC CCGAGAGCCT CACAGAGACA 
     1741 TCCACGCACG CATGAGACTC CACAACAACC GTGTGGGCAG GCAGGCGGTG ATGGAGAACA 
     1801 TGCGGCGAAA GTGCAAGTGC CACGGCACCT CAGGCAGCTG CCAGCTCAAG ACCTGCTGGC 
     1861 AGGTGACGCC GGAGTTCCGC ACAGTGGGGG CGCTGCTGCG CAACCGCTTC CACCGCGCCA 
     1921 CGCTCATCCG GCCGCACAAC CGCAACGGTG GCCAGTTGGA GCCCGGCCTC GCGGGAGCAC 
     1981 CCTCGCCAGC ACCGGGTACT CCAGGGCTGC GCCGTAGGGC CAGTCACTCC GACCTGGTCT 
     2041 ACTTTGAGAA ATCGCCCGAC TTCTGTGAGC GCGAGCCTCG TCTGGACTCG GCAGGCACTG 
     2101 TGGGGCGCCT GTGCAATAAG AGCAGCACGG GTCCAGACAG CTGCGGCAGC ATGTGCTGTG 
     2161 GCCGCGGTCA CAACATTCTG CGCCAGACGC GCAGCGAGCG CTGCCACTGT CGTTTCCACT 
     2221 GGTGCTGCTT CGTGGTCTGC GAAGAATGCC GCATCACTGA GTGGGTCAGC GTCTGCAAGC 
     2281 TGGTGCTGCT TCGTGGTCTG CGAAGAATGC CGCATCACTG AGTGGGTCAG CGTCTGCAAG 
     2341 AGCGGACCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA 
     2401 GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT 
     2461 CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT 
     2521 CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG 
     2581 CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG 
     2641 GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG 
     2701 CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT 
     2761 TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA 
     2821 ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC 
     2881 CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC 
     2941 CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA 
     3001 GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG 
     3061 CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC 
     3121 AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     3181 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     3241 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     3301 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     3361 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     3421 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     3481 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     3541 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     3601 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     3661 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     3721 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     3781 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     3841 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     3901 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     3961 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC 
     4021 CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG 
     4081 GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG 
     4141 AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT 
     4201 TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG 
     4261 TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA 
     4321 GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG 
     4381 CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC 
     4441 CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC 
     4501 GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC 
     4561 AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC 
     4621 ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC 
     4681 GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG 
     4741 TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC 
     4801 TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT 
     4861 AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC 
     4921 AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC 
     4981 CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG 
     5041 CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT 
     5101 GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC 
     5161 GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC 
     5221 TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT 
     5281 CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG 
     5341 CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT 
     5401 GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG 
     5461 CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC 
     5521 CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC 
     5581 CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG 
     5641 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 
     5701 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 
     5761 AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG 
     5821 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC 
     5881 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG 
     5941 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC 
     6001 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT 
     6061 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC 
     6121 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA 
     6181 ACAAAAATTT AACGCGAATT TT
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