LOCUS       RR211318                8373 bp    DNA     circular  SYN 
DEFINITION  Tjp2 (Myc-DDK-tagged ORF) - Rat tight junction protein 2 (Tjp2), (10 ug)
ACCESSION   NM_053773
VERSION     NM_053773.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4530..4621 
                     /label="Myc-DDK"  
     misc_feature    4651..5236 
                     /label="PolyA signal"  
     misc_feature    4708..4727 
                     /label="Restriction Enzyme"  
     misc_feature    5512..6184 
                     /label="ColE1"  
     misc_feature    6614..7408 
                     /label="Kan/Neo"  
     misc_feature    7443..7745 
                     /label="SV40 ori"  
     misc_feature    7831..8308 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4512..4518 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4512 
                     /label="Tjp2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGAGGTG ATATGGGAAC AGTACACTGT GACCCTGCAG AAGGATTCCA 
     1081 AAAGAGGATT TGGAATCGCA GTGTCAGGAG GCAGAGACAA CCCCCACTTT GAAAATGGGG 
     1141 AGACGTCAAT CGTCATTTCA GATGTACTCC CAGGTGGACC CGCTGATGGC TTGCTTCAAG 
     1201 AAAATGACAG GGTGGTCATG GTCAATGGCA CCCCCATGGA GGATGTGCTC CATTCATTTG 
     1261 CCGTTCAGCA GCTTAGGAAA AGTGGGAAGA TCGCAGCCAT TGTGGTCAAG AGGCCTCGGA 
     1321 AGGTTCAGGT GGCCCCGCTG CAGGGCAGCC CCCCTCTCAG TCATGATGAC CGAGGTTTTG 
     1381 AGATGATGGA TGAATTTGAT GGCAGAAGTT TCCGCAGCGG CTACAGTGAG AGGAGCCGGC 
     1441 ACAGCAGCCA CGATATGCTC AGCCACAGCT GGGAGGACAG CAGGGAGAGG GGGCGTCCCC 
     1501 ATCAGCGCGC ACAGAGCCGG GAGCGGGAGC GCAGCCGTGG CCGGAGCCTG GAACGTGGCC 
     1561 TGGACCATGA AGACTACGGG CGCAGCCATG AGCGCAGCCG CGGCCGGAGC CTGGAGCGCG 
     1621 GTCTAGACCG CGACTTTGTG TCCCGGGACT ACAGCCGTGG ACGGAGCATC GACAGGGACT 
     1681 ACGACCGAGA CTATGAGCGC TCCTATCACC AAGCCTATGA GCCTGACTAC GAAGGCCGCT 
     1741 ACAGCCCATC TTATGACCGC AGAGCCCATC CAGAGACCCG CTATGAACGG AGCCGCAGCC 
     1801 GAGAACACCT ACGGTCCCGA AGCCCCACCC CCGAGTCTAG GTCCCGGCAG GAACACAAGG 
     1861 GCCAGCATGA TCCCGACAGG CCCATCGGGG TCCTTCTGAC CAAAAGCAAA GCAAACGAAG 
     1921 AGTACGGGCT CCGGCTGGGA AGTCAGATCT TCATTAAGGA AATGACCAGA ACAGGACTGG 
     1981 CAACAAAAGA TGGCAACCTT CATGAGGGGG ACATAATTCT CAAGATCAAT GGAACTGTTA 
     2041 CTGAGAACAT GTCTTTAACC GATGCTCGGA AGTTAATAGA AAAGTCTCGA GGAAAACTGC 
     2101 AGCTGGTGGT GTTGAGAGAC AGCAAGCAGA CCCTCATCAA CATCCCATCT CTGAATGACA 
     2161 GCGACTCGGA AGTGGAGGAT ATCTCGGAAA TCGAGTCCAA CCGATCTTTT TCTCCAGAGG 
     2221 AGAGGCGCCA GCAGTATTCT GATCAGGAGT ATCATTCCTC CACTGAGAAG CTGAAGGAGA 
     2281 GGCCAAGCTC AAGAGAGGAG ACCTCAGGCA GAATGTCCAG GATGGGTGCC ACACCCACGC 
     2341 CGTTCAAGTC CACGGGGGAC ATCACAGCTG CAGGTGTCAC AGAGGCCAAC AAGGAGCCCA 
     2401 GGTCCCAGGA GGAATCCCCA GTTCCTCAAC CCAGAACAGC ATCAAGAGTC TTTCTTCGTC 
     2461 CTAGTCCCGA AGATGAAGCA ATATATGGCC CTAACACCAA AATGGTGAAG TTCAAGAAGG 
     2521 GAGACAGCGT GGGCCTCCGG TTGGCTGGTG GAAATGATGT TGGCATATTT GTGGCTGGCA 
     2581 TTCAGGAGGG CACCTCTGCA GAGCAGGAGG GCCTACAAGA AGGGGACCAG ATTCTGAAGG 
     2641 TGAACACACA AGATTTCAGA GGGCTGGTCC GGGAAGATGC CGTCCTCTAC CTGTTAGAAA 
     2701 TCCCTAAAGG TGAAACCGTG ACCATTTTGG CTCAGAGCCG AGCAGACGTG TATAGAGACA 
     2761 TCCTGGCCTG TGGCAGGGGA GACTCGTTCT TCATAAGGAG CCACTTTGAA TGTGAGAAGG 
     2821 AGACTCCGCA GAGCTTGGCC TTCACCAGGG GAGAGGTCTT CCGAGTGGTA GACACGCTGT 
     2881 ACGATGGCAA ACTGGGCCAC TGGCTGGCTG TGAGGATTGG AAATGAGCTG GAGAAGGGCT 
     2941 TAATCCCTAA CAAAAGCAGA GCTGAGCAGA TGGCCAGTGT CCAGAATGCC CAGCGAGAGA 
     3001 ATGCTGGGGA CAGAGCAGAC TTCTGGCGGA TGCGTGGCCA GAGATCCGGG GTCAAGAAGA 
     3061 ACATTCGCAA GAGCCGGGAA GACCTGGCAG CTGCTGTGTC GGTTAGCACC AAGTTCCCCG 
     3121 CCTACGAGAA GGTTCTGCTT CGGGAAGCTG GCTTCAAGAG ACCCGTGGTT CTGTTTGGCC 
     3181 CCATAGCAGA TATAGCAATG GAAAGGCTGA CTACTGAGCT ACCCGACCTG TTTCAAACTG 
     3241 CAAAAACAGA ACCCAAAGAT GCGGGATCTG AGAAATCCAG TGGAGTGGTT CGGTTGAATA 
     3301 CTGTGAGGCA AATTATTGAG CAGGACAAGC ATGCCCTGCT CGACGTTACC CCCAAAGCTG 
     3361 TGGACCTGCT CAATTATACT CAGTGGTTCC CAATCGTGAT TTTCTTCAAC CCGGATTCCA 
     3421 GACAAGGCAT TAAAACCATA AGGCAGAGGT TGAACCCAAC ATCCAATAAA AGTTCTCGCA 
     3481 AGTTATTCGA TCAAGCCAAC AAGCTCAAAA AAACCTGTTC TCATCTTTTT ACAGCTACCA 
     3541 TCAACGTGAA TTCAGCCAAT GATGGCTGGT TTGGCAGCCT GAAGGACAGC ATTCAGCAGC 
     3601 AGCAAAACGA AGCAGTGTGG GTCTCTGAAG GAAAGATGGA GGGGATGGAT GATGACGCTG 
     3661 AAGACCGCAT GTCCTACTTA ACCGCCATGG GTGCGGACTA TCTGAGTTGT GACAGCCGTC 
     3721 TCATCAGTGA CTTTGAAGAT ACGGACGGCG AGGGAGGCGC CTACACTGAC AATGAGCTGG 
     3781 ATGAGCCAGC TGAGGAGCCG CTGGTGTCTT CCATCACCCG CTCCTCAGAG CCGGTGCAGC 
     3841 ATGAGGAGAG CATAAGGAAG CCCAGCCCAG AGCCACGCGC TCAGATGAGG AGGGCAGCTA 
     3901 GCAGAGACCA GCTTAGGGAT GGTAGCCCGC CCCCAGCATT CAAGCCAGAG CCGCCCAAGG 
     3961 TCAGAAACCA AAACAGAGAG GACTCTTTCA ACTACTCCAA GTCAAACCTC TCTGCCATGG 
     4021 CTGGCAGTGA AATCCCGGGG GGATCCACCA AAGGGTGTCC TCCCCCTATT GCGGTGAAAC 
     4081 CTGCCTTTGG GCGATCCATC CTGAAGCCTT CTACTCCAGT CCCCATGCCT GAGAGTGAGG 
     4141 AGGTTGGGGA GAGCACCGAG GAGCAGGAAG AGGCTCCCAA ATCAGTCCTG GGCAGAGTGA 
     4201 AAATCTTCGA GAAGATGGAC CACAAGGCGA AATTACAGAG GATGCAGGAG CTCCAAGAAG 
     4261 CACAGAATGC GAGGATTGAA ATAGCTCAGA AGCATCCTGA CATCTATGCG GTTCCAATCA 
     4321 AAGCCCCCAA GCCAGATGCT GGCCTGCCCC AGCACATGAG ACCCCCAGAG CCACAGAAAG 
     4381 CTCCTTCTAG GCTTTACCAG GACACCAGAG GAAGCTACGG CAGTGATCCC GAGGAAGAGG 
     4441 AGGAGTACCG CCAACAGTTG GCAGCACACT CGAAGCGTGG TTACTACAGC CAGCCCTCCC 
     4501 GGTACCGAGA CACCGAATTA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4561 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4621 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4681 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4741 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4801 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4861 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4921 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4981 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5041 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5101 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5161 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5221 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5281 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5341 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5401 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5461 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5521 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5581 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5641 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5701 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5761 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5821 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5881 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5941 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6001 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6061 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6121 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6181 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6241 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6301 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6361 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6421 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6481 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6541 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6601 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6661 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6721 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6781 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6841 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6901 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6961 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7021 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7081 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7141 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7201 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7261 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7321 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7381 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7441 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7501 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7561 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7621 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7681 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7741 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7801 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7861 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7921 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7981 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8041 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8101 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8161 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8221 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8281 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8341 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//