LOCUS       RR211255                8454 bp    DNA     circular  SYN 
DEFINITION  Smc2 (Myc-DDK-tagged ORF) - Rat structural maintenance of chromosomes 2 (Smc2), (10 ug)
ACCESSION   NM_001108666
VERSION     NM_001108666.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4611..4702 
                     /label="Myc-DDK"  
     misc_feature    4732..5317 
                     /label="PolyA signal"  
     misc_feature    4789..4808 
                     /label="Restriction Enzyme"  
     misc_feature    5593..6265 
                     /label="ColE1"  
     misc_feature    6695..7489 
                     /label="Kan/Neo"  
     misc_feature    7524..7826 
                     /label="SV40 ori"  
     misc_feature    7912..8389 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4593..4599 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4593 
                     /label="Smc2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTATGTTAAA TCAATCATTC TCGAAGGATT CAAGTCCTAT GCACAAAGGA 
     1081 CTGAAGTCAA TGGTTTTGAC CCCCTCTTCA ATGCGATCAC TGGTTTGAAT GGTAGTGGAA 
     1141 AATCCAATAT ATTGGATTCC ATATGCTTTC TCCTAGGCAT CTCCAACCTG TCTCAGGTTC 
     1201 GGGCTTCAAA TTTACAAGAT CTGGTTTATA AAAACGGACA GGCTGGAATC ACCAAAGCCT 
     1261 CTGTTTCAAT CACTTTTGAT AATTCTGACA AAAAGCAGAG TCCATTAGGA TTTGAAGCTC 
     1321 ATGATGAAAT CACTGTAACA AGACAGGTGG TTATTGGTGG CAGAAATAAA TACTTAATCA 
     1381 ATGGAGTGAA TGCCAACAAC ACCAGAGTCC AGGATCTCTT TTGTTCTGTG GGCCTGAACG 
     1441 TGAATAACCC TCACTTTCTC ATCATGCAGG GCAGAATTAC GAAAGTATTA AATATGAAAC 
     1501 CACCAGAGAT ATTATCCATG ATCGAAGAAG CTGCTGGAAC CAGGATGTAT GAGTACAAAA 
     1561 AAATAGCTGC CCAGAAAACT ATAGAAAAAA AGGAGGCTAA GCTGAAAGAA ATAAAAACGA 
     1621 TACTGGAAGA AGAGATTACG CCAACAATTC AAAAATTAAA AGAGGAAAGA TCTTCTTACT 
     1681 TGGAGTACCA GAAAGTGATG CGTGAGATCG AGCATCTGAG CCGTTTATAT ATTGCCTATC 
     1741 AGTTTTTGCT GGCTGAAGAT ACCAAGGAAC GCTCAGCTGG GGAGCTAAAG GAAATGCAGG 
     1801 ATAAAATATT AAAGCTTCAA GAAGTACTGT CAGAGAATGA AAAAAAGATA AAGGCTCTCA 
     1861 ATTGTGAAAT AGAAGAATTG GAGAAGAGAA AAGATAAGGA AACTGGAGGC ATCCTCCGAT 
     1921 CTTTAGAGGA TGCTTTTGCA GAAGCTCAGC GAGTTAATAC TAAGTCTCAA AGTGCATTTG 
     1981 ACCTCAAGAA GAAAAACCTA GCCAGTGAAG AAACCAAGCG CAAAGAGCTA GAGAAAAACA 
     2041 TGGCTGAGGA CTCTAAAGCA TTAGCAGCAA AGGAAAAGGA AGTTAAGAAG CTAACCGATG 
     2101 GACTGCACGG CCTTCAAGAA GCAAGCAATA AAGATGCGGA AGCTCTGGCC GCCGCCCAGC 
     2161 AGCACTTCAA TGCAGTGTCT GCTGGCTTGT CCAGTAATGA AGATGGAGCA GAGGCAACTC 
     2221 TGGCCGGGCA GATGATGGCC TGTAAGAACG ACATCAGCAA AGCGCAGACC GGGGCCAAGC 
     2281 AGGCTCAGAT GAAGTTGAAA CATGCCCAAC AGGAACTGAA GAATAAACAA GCAGAAGTTA 
     2341 GGAAGATGGA TAGTGGGTAC AAGAAGGATC AAGAAGCTTT TGAAGCTGTG AAAAAAGTTA 
     2401 AAGAAAAACT TGAAACCGAA ATGAAGAAGC TAAATTACGA AGACAACAAA GAGGAAAGGC 
     2461 TTTTGGAAAA GCACAGGCAG GTGTCTCGTG ACATCAGTAA TCTAAAAGGA ACCTACGAAG 
     2521 CTCTCTTGGC CAAGTTTCCC AATCTTCGAT TTGCATACAA GGACCCAGAG AAGAACTGGA 
     2581 ATCGAAATTG TGTAAAAGGA CTTGTAGCTT CTCTGATAAA TGTGAAAGAT AATTCTACAG 
     2641 CCACAGCTCT GGAATTGGTG GCTGGAGAAC GACTCTACAA TGTTGTAGTA GACACAGAGG 
     2701 TTACTGGTAA GAAACTATTA GAAAAGGGGG AATTGAAGCG TCGATACACA ATAATTCCTC 
     2761 TCAATAAGAT TTCAGCCAGA TGTATTGCTC CTGAAACTCT GAGGGTCGCT CAGAATCTTG 
     2821 TTGGTCCTAA CAATGTTCAT GTAGCTCTTT CCTTGGTTGA CTACAAACCA GAACTTCAGA 
     2881 AAGCGATGGA ATTTGTCTTC GGAACAACAT TTGTTTGTAA CAATATGGAT AATGCCAAAA 
     2941 AAGTGGCCTT TGATAAAAGA ATAATGACCA GAACTGTAAC CCTTGGGGGT GATGTATTTG 
     3001 ATCCTCATGG AACATTGAGT GGAGGTGCTA GGTCCCAGGC GGCTTCTATT TTAACCAAGT 
     3061 TTCAAGAACT CAAAGATGTT CAAGCTGAGC TGAGGACAAA GGAGAAAGAA CTGCAGGCTC 
     3121 TGGAAGAGGA ATTGGCTGGT CTTAAAAATG TCGCTGAAAA GTATCGCCAA CTGAAACAGC 
     3181 AGTGGGAGAT GAAGACAGAG GAGGGAGATT TGTTGCAAAC CAAACTTCAG CAAAGCTCTT 
     3241 ACCATAAGCA GCAAGAAGAA TTAGATGCCC TTAAAAAAAC CATTGAGGAA AGTGAGGAGA 
     3301 CATTAAAAAA CACAAAAGAA ATCCAAAAGA AAGCTGAAGA AAAATATGAG GCATTGGAGA 
     3361 ATAAAATGAA AAATGCAGAA GCTGAAAGAG AGAAAGAACT GAAGGATGCT CAGAAAAAAC 
     3421 TGGATTGTGC CAAAACAAAG GCTGATGCAT CTAGCAAGAA AATGAAAGAA AAGCAACAGG 
     3481 AGGTTGAAGC TATCACTCTG GAGCTAGAGG AGCTCAAAAG AGAGCATGCA TCTAACGAAC 
     3541 AGCAGCTTGA TGCTGTGAAT GAAGCCATCA AAGCCTATGA AGGGCAGATT GAAATAATGG 
     3601 CAGCTGAGGT GGCCAAAAAC AAGGAATCAG TTAATAAAGC TCAAAATGAG CTGATGAAGC 
     3661 AAAAAGAAAT AATAAGTGCC CAGGACAACA TAATTAAAGA TAAATGTGCG GAAGTGGCAA 
     3721 AACACAATTT GCAAAACAAT GATTCTCAGC TTAAAATTAA GGAGTTAGAC CACAGCATAA 
     3781 GCAAACACAA GCGGGAAGCT GACGACGCTG CTGCGAAGGT ATCCAAAATG TTGAATGATT 
     3841 ATGACTGGAT TAATGCTGAG AAACACCTCT TTGGCCAACC TAATAGTGCC TATGATTTCA 
     3901 AAACAAACAA CCCCAAAGAA GCTGGCCAGA GACTTCAGAA GTTGCAAGAA GTAAAAGAGA 
     3961 AACTAGGGAG AAATGTCAAC ATGAGAGCTA TGAATGTGCT GACAGAAGCG GAAGAGCGGT 
     4021 ATAATGATTT GATGAAGAAG AAAAGAATTG TAGAAAATGA CAAATCCAAA ATCCTTGCAA 
     4081 CTATAGAAGA TCTTGACCAG AAGAAAAATC AGGCCCTAAA TATTGCTTGG CAAAAGGTGA 
     4141 ACAAAGACTT TGGCTCTATT TTTTCTACCC TTTTGCCTGG TGCCAATGCT ATGCTTGCAC 
     4201 CACCAGAAGG GCAGACAGTT TTAGATGGTC TGGAGTTCAA GGTTGCCTTA GGAAATACAT 
     4261 GGAAAGAAAA CCTAACTGAG CTTAGTGGTG GTCAGAGGTC TCTGGTGGCT CTCTCACTAA 
     4321 TATTGTCCAT GCTCCTCTTC AAACCTGCTC CAATTTATAT CTTGGACGAG GTGGATGCGG 
     4381 CCTTAGATCT TTCTCATACC CAGAACATCG GACAGATGCT GCGAACTCAC TTCACACATT 
     4441 CTCAATTCAT TGTGGTATCC CTCAAAGAAG GTATGTTCAA CAATGCAAAC GTTCTCTTCA 
     4501 AAACCAAGTT TGTGGATGGT GTTTCTACAG TAGCCAGATT TACACAGAGT CAAAATGGGA 
     4561 GGATTCCAAA GGAGGCAAAG GCCAAGGGCA AAGGACCCAG CACGCGTACG CGGCCGCTCG 
     4621 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4681 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4741 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4801 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4861 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4921 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4981 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5041 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5101 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5161 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5221 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5281 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5341 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5401 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5461 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5521 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5581 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5641 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5701 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5761 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5821 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5881 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5941 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6001 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6061 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6121 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6181 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6241 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6301 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6361 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6421 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6481 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6541 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6601 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6661 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6721 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6781 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6841 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6901 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6961 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7021 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7081 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7141 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7201 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7261 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7321 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7381 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7441 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7501 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7561 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7621 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7681 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7861 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7921 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7981 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8041 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8101 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8161 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8221 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8281 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8341 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8401 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//