LOCUS       RR211197                8013 bp    DNA     circular  SYN 
DEFINITION  Myo1c (Myc-DDK-tagged ORF) - Rat myosin IC (Myo1c), (10 ug)
ACCESSION   NM_023092
VERSION     NM_023092.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4170..4261 
                     /label="Myc-DDK"  
     misc_feature    4291..4876 
                     /label="PolyA signal"  
     misc_feature    4348..4367 
                     /label="Restriction Enzyme"  
     misc_feature    5152..5824 
                     /label="ColE1"  
     misc_feature    6254..7048 
                     /label="Kan/Neo"  
     misc_feature    7083..7385 
                     /label="SV40 ori"  
     misc_feature    7471..7948 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4152..4158 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4152 
                     /label="Myo1c"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCTACCGG GCGTCGGCCC TGGGCAGTGA CGGGGTACGA GTGACCATGG 
     1081 AGAGCGCCTT GACTGCCCGA GACCGGGTGG GGGTGCAAGA CTTTGTTCTG CTGGAGAACT 
     1141 TCACCAGTGA GGCTGCCTTC ATTGAGAACC TCCGGCGTCG ATTCCGGGAG AACCTCATTT 
     1201 ATACCTACAT TGGCCCTGTC CTGGTCTCTG TCAACCCCTA CCGGGACCTA CAGATCTACA 
     1261 CACGGCAACA TATGGAACGC TACCGTGGCG TCAGTTTCTA TGAAGTGCCG CCTCATCTGT 
     1321 TTGCAGTGGC TGACACTGTA TACCGGGCGC TTCGTACTGA GCGTCGGGAC CAAGCAGTGA 
     1381 TGATTTCTGG AGAGAGTGGG GCAGGGAAGA CAGAGGCCAC CAAGAGATTG CTCCAGTTCT 
     1441 ATGCAGAGAC CTGCCCAGCC CCTGAAAGGG GTGGCGCAGT GCGAGACCGC CTGTTGCAGA 
     1501 GCAACCCTGT GCTGGAGGCC TTTGGGAATG CCAAGACTCT CCGCAACGAC AACTCCAGCC 
     1561 GCTTTGGGAA GTACATGGAT GTGCAGTTTG ACTTCAAGGG TGCCCCTGTG GGAGGCCACA 
     1621 TTCTCAGTTA CCTCCTGGAG AAGTCTCGAG TGGTACACCA GAATCACGGA GAACGGAACT 
     1681 TCCACGTCTT TTACCAGCTC CTGGAGGGGG GGGAGGAGGA GGCTCTTCGT CGTCTGGGCT 
     1741 TGGAACGGAA CCCCCAGAGC TACTTGTACC TGGTGAAGGG CCAGTGTGCC AAGGTCTCCT 
     1801 CCATCAATGA CAAGAGTGAC TGGAAGGTTG TGAGGAAGGC GCTGTCAGTC ATTGACTTCA 
     1861 CTGAGGATGA AGTGGAGGAC TTGCTAAGCA TCGTGGCCAG CGTCCTACAT CTGGGCAATA 
     1921 TCCACTTTGC TGCTGATGAG GACAGCAATG CCCAGGTTAC TACTGAGAAC CAGCTCAAAT 
     1981 ATCTGACCAG GCTCCTTGGT GTGGAAGGCA CAACACTGAG GGAAGCCCTG ACCCACAGGA 
     2041 AGATCATCGC CAAGGGGGAA GAGCTCCTGA GCCCACTGAA CCTTGAACAG GCTGCATATG 
     2101 CAAGGGATGC TCTTGCCAAG GCTGTGTACA GCCGCACTTT CACCTGGTTG GTCAGAAAGA 
     2161 TCAATAGGTC TCTGGCCTCT AAGGACGCTG AGAGCCCCAG CTGGCGAAGC ACCACAGTTC 
     2221 TTGGGCTCCT GGACATTTAT GGCTTCGAAG TGTTTCAGCA TAACAGCTTC GAGCAGTTCT 
     2281 GCATCAACTA TTGCAATGAG AAGCTGCAGC AGCTCTTCAT TGAGCTGACT CTCAAGTCCG 
     2341 AGCAGGAGGA ATACGAGGCA GAGGGCATCG CGTGGGAACC TGTCCAATAC TTCAACAACA 
     2401 AGATCATCTG TGACCTGGTG GAGGAGAAAT TCAAGGGCAT CATCTCCATC TTGGATGAAG 
     2461 AGTGCCTGCG TCCTGGGGAG GCCACAGACC TGACCTTCCT GGAGAAGCTG GAGGACACAA 
     2521 TCAAGCACCA CCCTCACTTC CTGACGCACA AGCTTGCTGA CCAGAAGACC AGGAAATCCC 
     2581 TCGACCGAGG GGAGTTCCGC CTTCTTCATT ATGCGGGAGA GGTGACCTAC AGCGTGACTG 
     2641 GGTTTCTGGA TAAAAACAAT GACCTCCTCT TCCGGAACCT GAAGGAGACC ATGTGCAGCT 
     2701 CCACGAACCC CATCATGGCC CAGTGCTTTG ACAAGAGTGA GCTCAGTGAC AAGAAGCGGC 
     2761 CAGAGACGGT GGCCACCCAG TTCAAGATGA GCCTCTTGCA GCTAGTGGAG ATCCTGAGGT 
     2821 CTAAGGAACC TGCTTATATC CGGTGCATCA AGCCAAATGA CGCCAAACAG CCGGGTCGCT 
     2881 TTGATGAGGT GCTCATCCGA CACCAAGTGA AGTACCTGGG ACTTATGGAG AATCTGCGTG 
     2941 TGCGCAGAGC TGGCTTTGCC TACCGTCGAA AATATGAGGC TTTCCTGCAG AGGTACAAGT 
     3001 CACTGTGCCC AGAGACATGG CCCGTGTGGA CAGGACGGCC CCAGGATGGG GTGGCTGTGT 
     3061 TGGTCAGACA CCTTGGCTAT AAGCCAGAAG AGTACAAAAT GGGCAGGACT AAGATCTTCA 
     3121 TCCGATTCCC CAAGACCTTG TTTGCCACAG AGGACTCCCT GGAAGTCCGA CGGCAGAGTC 
     3181 TAGCCACCAA GATCCAGGCG GCGTGGAGGG GCTTTCATTG GCGGCAGAAA TTTCTCCGGG 
     3241 TGAAGCGATC AGCCATCTGT ATCCAGTCCT GGTGGCGCGG TACACTGGGC CGGAGGAAGG 
     3301 CAGCCAAAAG GAAGTGGGCA GCCCAGACTA TCCGTAGACT CATCCGTGGC TTCATTTTGC 
     3361 GTCATGCACC CCGGTGCCCT GAGAATGCCT TCTTCCTGGA CCACGTGCGC ACGTCATTTT 
     3421 TGCTTAACCT GAGGCGACAA CTACCCCGGA ATGTTCTGGA CACCTCCTGG CCCACACCCC 
     3481 CACCTGCCCT GAGAGAGGCC TCGGAGCTGC TCCGGGAGCT ATGCATGAAG AACATGGTGT 
     3541 GGAAATACTG CCGGAGTATC AGCCCTGAGT GGAAGCAGCA GCTGCAGCAA AAGGCGGTGG 
     3601 CTAGTGAAAT TTTCAAGGGC AAGAAGGACA ATTACCCCCA GAGTGTCCCC AGACTTTTCA 
     3661 TCAGCACACG GCTTGGCACA GAGGAGATCA GCCCCAGAGT GCTTCAGGCC TTGGGCTCTG 
     3721 AACCCATCCA GTATGCAGTG CCTGTGGTAA AATACGACCG TAAGGGCTAC AAGCCTCGTT 
     3781 CCCGGCAGCT GCTGCTCACA CCCAGTGCTG TGGTCATCGT GGAGGATGCC AAAGTCAAGC 
     3841 AGAGAATTGA TTATGCCAAC CTAACTGGAA TCTCCGTCAG TAGCCTGAGT GATAGCCTGT 
     3901 TTGTGCTTCA CGTGCAGCGT GAAGACAGCA AGCAGAAGGG AGATGTGGTA CTGCAGAGCG 
     3961 ATCATGTGAT CGAGACACTA ACCAAGACGG CCCTCAGTGC TGACCGTGTG AATAATATCA 
     4021 ACATCAACCA GGGCAGCATC ACGTTTGCAG GGGGTCCAGG CAGGGATGGC ATCATCGACT 
     4081 TCACATCTGG CTCAGAGCTT CTCATCACCA AGGCTAAGAA TGGCCACCTG GCTGTGGTGG 
     4141 CCCCACGGCT GAATTCTCGG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4201 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4261 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4321 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4381 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4441 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4501 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4561 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4621 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4681 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4741 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4801 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4861 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4921 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4981 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5041 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5101 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5161 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5221 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5281 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5341 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5401 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5461 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5521 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5581 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5641 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5701 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5761 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5821 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5881 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5941 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6001 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6061 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6121 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6181 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6241 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6301 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6361 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6421 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6481 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6541 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6601 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6661 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6721 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6781 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6841 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6901 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6961 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7021 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7081 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7141 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7201 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7261 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7321 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7381 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7441 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7501 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7561 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7621 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7681 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7741 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7801 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7861 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7921 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7981 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//