LOCUS       RR211192                7782 bp    DNA     circular  SYN 
DEFINITION  Rgs3 (Myc-DDK-tagged ORF) - Rat regulator of G-protein signaling 3 (Rgs3), (10 ug)
ACCESSION   NM_019340
VERSION     NM_019340.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3939..4030 
                     /label="Myc-DDK"  
     misc_feature    4060..4645 
                     /label="PolyA signal"  
     misc_feature    4117..4136 
                     /label="Restriction Enzyme"  
     misc_feature    4921..5593 
                     /label="ColE1"  
     misc_feature    6023..6817 
                     /label="Kan/Neo"  
     misc_feature    6852..7154 
                     /label="SV40 ori"  
     misc_feature    7240..7717 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3921..3927 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3921 
                     /label="Rgs3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACCGCTTC AATGGGCTCT GCAAAGTGTG CTCAGAACGC CGCTACAGGC 
     1081 AGATCACCAT CCGGAGGGGA AAAGACGGCT TTGGCTTCAC CATCTGCTGT GACTCTCCGG 
     1141 TCCGAGTCCA GGCTGTGGAT TCTGGGGGCC CAGCAGAGAG GGCGGGGCTG CAGCAGCTGG 
     1201 ACACAGTACT ACAGCTGAAT GAGAGGCCCG TGGAACACTG GAAATGTGTG GAGCTGGCAC 
     1261 ACGAGATCCG GAGCTGTCCC AGCGAGATCA TCCTGCTCGT GTGGCGTGTG GTCCCCCAGA 
     1321 TCAAGCCAGG GCCAGATGGC GGAGTCTTGA GGCGGGCCTC CTGCAAGTCC ACACATGATC 
     1381 TCCTGTCACC CCCTAACAAG AGGGAGAAGA ACTGTACTCA CGGGGCTCCA ACACGTCCCG 
     1441 AGCAGCGCCA CAGCTGCCAC CTGGTGTGCG ACAGCTCTGA TGGGCTACTG CTCGGAGGCT 
     1501 GGGAGCGCTA CACTGAGGTG GGCAAGCGCA GCGGCCAGCA TACCCTGCCT GCACTGTCCC 
     1561 GGGCCACCAC CCCTACTGAC CCCAACTACA TCATCCTGGC CCCACTGAAT CCTGGAAGCC 
     1621 AGTTGCTGCG GCCTGTGTAC CAGGAGGATG CCATCCCTGA AGAACCAGGG ACTACTACTA 
     1681 AAGGGAAATC GTACACTGGC CTGGGCAAGA AGTCTCGGTT GATGAAGACA GTGCAGACCA 
     1741 TGAAGGGCCA CAGCAACTAC CAAGATTGCT CAGCCCTGAG ACCACACATC CCACATTCAA 
     1801 GTTACGGCAC CTATGTCACC CTGGCCCCCA AAGTCCTGGT ATTCCCTGTC TTTGTGCAGC 
     1861 CCCTAGATCT CTGTAACCCT GCCCGGACTC TCCTGCTGTC AGAGGAGCTG CTGCTCTATG 
     1921 AGGGGAGGAA CAAGACTTCC CAGGTGACAC TGTTTGCCTA CTCGGACCTG CTGCTGTTCA 
     1981 CTAAGGAGGA GGAACCAGGC CGCTGTGACG TCCTGAGAAA CCCCCTCTAC CTCCAGAGTG 
     2041 TGAAGCTACA GGAGGGTTCT TCAGAAGACT TGAAATTCTG TGTGCTGTAC CTGGCAGAGA 
     2101 AGGCAGAGTG CTTATTCACT TTGGAGGCTC ACTCGCAGGA GCAGAAGAAG AGAGTGTGCT 
     2161 GGTGCCTGTC GGAGAACATC GCCAAGCAGC AACAGCTGGC TGCAACACCT ACGGAGAGGA 
     2221 AGATGTTTGA GACAGAGGCA GATGAGAAGG AGATGCCCCT GGTCGAGGGG AAGGGGCCAG 
     2281 GTGCTGAGGA ACGCACACCC AGCAAAGACC CCTCTCCCAG CCAGGAGCTG CCTCCAGGAC 
     2341 AAGAACTCCC ACCCAGCAAA GACCCCTCTC CCAGCCAGGA GCTGCCTCCA GGACAAGAAC 
     2401 TCCCACCCAG CAAAGACCCC TCTCCCAGCC AGGAGCTGCC TCCAGGACAA GAACTCCCAT 
     2461 CCAGCAAAAA CCCCTCTCCC AGCCAGGAGC TTCCTGCAGG ACAAGACCTC CCACCCAGGA 
     2521 AGGAATCCTT TTCAGGCCAA GAAGCAGCTC CTGGCCCAGA GTCACCATCC AGTGAAGACA 
     2581 TAGCAACCTG CCAAAACCCC CCTCAAAGCC CAGAAACCTC AACAAGCAAG GACTCCCCAC 
     2641 CAGGCCAGGG ATCCTCTCCA ACCACAGAAG TCCCATCTTG CCAGGGACTT CCTGCTGGTC 
     2701 AAGAATCTAC AAGCCAGGAC CCTCTGCTCA GTCAAGAGCC CCCTGCTATC CCAGAATCCT 
     2761 CTGCCTCCGA TCAGAATGTT CTACCCTCTC AGGAGTCACC TCCCAGCCAG GGCTCCCTAT 
     2821 CAGAGAAGGC CCTTGCTGAG CAGACCATCA GCCCCGGGGA GCTACCAGCG GCCACTGCAG 
     2881 GTGAACCTTC AGCCTCCAGA CCTAATTTTG TGATCCCCGA GGTCCGCCTG GATAGTGCCT 
     2941 ACAGCCAGCA GGATGGAGCC CATGGAGGCA GCTCTGGTGA GGATGAGGAT GCAGAAGAGG 
     3001 GAGAGGAGGG GGAAGAAGGG GAGGAAGATG AGGAGGACGA CACCAACGAC GACAACTACG 
     3061 GGGATCGTAA TGAGGCCAAG CGCAGCAGCC TGATTGAGAC TGGCCAGGGC GCCGAGGGTG 
     3121 GCCTCTCGTT GCGTGTGCAG AACTCGCTGC GGCGCCGGAC GCACAGCGAG GGCAGCCTGC 
     3181 TGCAGGAGGC CCGTGGGCCC TGCTTTGCCT CTGACACCAC CCTACACTGC TCTGATGGTG 
     3241 AGGGCACCAC ATCCACCTGG GCCATCCCTT CTCCCCGCAC CCTCAAAAAA GAACTGGGTC 
     3301 GTAATGGAGG CTCCATGCAC CACCTTTCCC TGTTCTTCAC TGGGCACAGG AAGATGAGTG 
     3361 GGACTGACCT CGCAGATGAC GTTGAAGCCT CCCGGAAGAG AAAGAGCAAA AACATAGCCA 
     3421 AGGATATGAA GAATAAGCTG GCCATCTTCA GGCGGCGGAA TGAATCTCCT GGGGCCCAGC 
     3481 CAGCCGGCAA GGCAGACAAG ACAACCAAGT CCTTCAAGCC TACCTCCGAG GAAGCCCTCA 
     3541 AGTGGAGCGA GTCCCTGGAA AAGCTGCTGC TTCATAAATA TGGCTTAGAA GTGTTCCAGG 
     3601 CCTTCCTTCG CACAGAGTTC AGTGAGGAGA ACCTGGAGTT TTGGCTGGCT TGTGAGGATT 
     3661 TCAAGAAGGT CAAATCGCAG TCCAAGATGG CAGCCAAAGC CAAGAAGATC TTTGCTGAGT 
     3721 TCATCGCGAT CCAGGCTTGC AAGGAGGTAA ACCTGGACTC GTACACACGA GAACACACTA 
     3781 AGGAGAACCT GCAGAGCATC ACCCGAGGCT GCTTTGACCT GGCACAAAAA CGTATCTTCG 
     3841 GGCTCATGGA AAAGGACTCT TACCCTCGCT TCCTCCGCTC TGACCTCTAC CTGGACCTCA 
     3901 TTAACCAGAA GAAGATGAGT CCCCCACTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3961 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4021 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4081 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4141 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4201 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4261 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4321 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4381 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4441 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4501 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4561 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4621 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4681 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4741 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4801 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4861 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4921 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4981 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5041 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5101 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5161 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5221 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5281 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5341 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5401 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5461 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5521 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5581 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5641 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5701 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5761 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5821 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5881 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5941 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6001 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6061 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6121 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6181 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6241 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6301 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6361 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6421 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6481 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6541 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6601 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6661 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6721 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6781 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6841 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6901 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6961 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7021 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7081 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7141 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7201 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7261 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7321 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7381 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7441 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7501 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7561 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7621 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7681 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7741 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//