LOCUS       RR211191                8955 bp    DNA     circular  SYN 
DEFINITION  Phlpp2 (Myc-DDK-tagged ORF) - Rat PH domain and leucine rich repeat protein phosphatase-like (Phlppl), (10 ug)
ACCESSION   NM_001109131
VERSION     NM_001109131.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5112..5203 
                     /label="Myc-DDK"  
     misc_feature    5233..5818 
                     /label="PolyA signal"  
     misc_feature    5290..5309 
                     /label="Restriction Enzyme"  
     misc_feature    6094..6766 
                     /label="ColE1"  
     misc_feature    7196..7990 
                     /label="Kan/Neo"  
     misc_feature    8025..8327 
                     /label="SV40 ori"  
     misc_feature    8413..8890 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5094..5100 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5094 
                     /label="Phlpp2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGAGGTG GAGCCCGTGC CCGCGGGCTC GCTGGAGCCC CCGGAGCCAC 
     1081 CTGAAGCGGC GGCGCCTCGC CGGCCCGGAG GTATTCGGGT CCTAAAGAGG AATATGAAAC 
     1141 ACAATGGGAG CAGAAATTGT TTGAATAGAA GAAGTAGGTT TGGCTCTCGA GAAAGAGACT 
     1201 GGCTAAGAGA AGATGTGAAG AGAGGCTGTG TTTACCTTTA TGGAGCGGAC ACGACCACTG 
     1261 CCACTACAAC CACCACCTCC TCCTCCTCCT CCTCCTCCTC CTCCTCCTCC TCTGATTTAC 
     1321 ATCTTGTCCT TTGCACAGTG GAGACACCAG CGTCAGAAAT ATGTGCTGGA GAGGGAAGAG 
     1381 AAAGTCTCTA TCTACAGCTT CATGGAGATC TGGTCAGGAG ACTGGAGCCC ACTGAACGGC 
     1441 CTCTCCAGAT TGTTTACGAC TACTTATCCA GGCTGGGGTT TGAAGATCCC GTGCGCATAC 
     1501 AGGAGGAGGC TACGAACCCT GATCTCAGCT GTATGATTCG ATTTTATGGT GAAAAGCCAT 
     1561 GCCAGATGGA TCATCTGGAC CGAATCCTGC TGTCTGGCAT CTATAACGTC CGCAAAGGGA 
     1621 AGACCCAGCT GCACAAATGG GCTGAGCGCC TCGTTGTTCT CTGTGGCACC TGCCTTATTG 
     1681 TCTCCTCAGT GAAGGATTGC CAAACTGGAA AGATGCACAT CTTGCCACTG GTTGGGGGAA 
     1741 AGATAGAAGA AGTGAAGCGC CGGCAACACT CCCTTGCTTT CAGCTCAGCT GGAGCCCAAG 
     1801 CTCAGACCTA TCATGTCAGC TTCGAGACTT TGGCTGAGTA CCAGAGATGG CAGCGGCAGG 
     1861 CGTCTAAGGT GGTGTCCCAG AGGATCAGCA CCGTGGACCT CTCGTGTTAC AGCCTTGAGG 
     1921 AGGTTCCTGA GCATCTCTTC TACAGTCAAG ACATCACCTA CCTCAACCTG CGACACAACT 
     1981 TCATGCAGTT AGAAAGACCA GGGGGCCTTG ACACACTCTA CAAATTTTCT CAACTGAAGG 
     2041 GCCTGAACTT GTCCCATAAT AAGCTTGGAC TGTTTCCTGT GTTGCTGTGT GAGATTTCTA 
     2101 CCCTGACTGA ACTCAACCTT TCTTGTAATG GATTTCATGA CTTGCCAAGT CAGATTGGTA 
     2161 ATCTGTTAAA TCTTCAGACC CTCTGCCTTG ATGGCAATGT TCTCACAGCT CTACCAGATG 
     2221 AATTGGGAAA CCTGCAGCAG CTTACTTCTC TGGGAATTTC CTTCAACAAT TTTAGTCAAA 
     2281 TTCCTGAAGT TTTGGAGAAA CTCACCATGT TAGATAAAGT GGTTATGGCA GGAAACCGTT 
     2341 TGGAAATCCT GAACCTAGGG GTGCTGACCA GGATGAACCA TGTTAAGCAT GTGGATCTAA 
     2401 GGATGAACCA CTTGAAAACG GTGATTATTG AAAATCTGGA GGGAAATAAA CACATCACTC 
     2461 ACATGGATTT GCGGGACAAT CAGTTGACTG ATTTGGACCT TAGCTCCTTA TGTAGCTTGG 
     2521 AGCAGCTGCA TTGTGAGCGG AACCAGTTGA GAGAGCTGAC ACTCAGTGGC TTCTCCCTTC 
     2581 GAAACCTCTA CGCCAATTGG AACAAGCTGA CAGCAGTGAA TGTCTATCCA GTACCAAGCC 
     2641 TGCTCACTTC TTTGGAACTC TCCCGAAACT TGCTAGAATG TATCCCTGAC TGGGCCTGTG 
     2701 AAGCAAAAAA GCTAGAAATA CTAGATATAA GCTACAATCT GCTTACAGAG GTTCCCATGA 
     2761 GAATTCTGAG TAGTCTGAGT CTTCGGAAAC TGATGGTAGG ACACAACCAC ATCCATGTCC 
     2821 TTCCGGCACT GGTGGAGCAC ATTCCCCTCG AGGTACTGGA TGTCCAGCAT AACACTCTGT 
     2881 CCAGGCTGCC GGACACACTG TTCTCCAAGG CCTTAAATCT CAGGTACTTG AATGCATCCG 
     2941 CAAATAGTCT GGAGTCTTTG CCTTCTGCGT GTGCTGGAGA GGAGAGTCTG AGTGTGCTGC 
     3001 AGCTGCTGTA CCTGACCAAC AACCTTCTGA CAGACCAGTG CATACCTGTT TTGGTCGGGC 
     3061 ACCCACACCT GCGTGTTCTG CACCTTGCAA ACAACCAGCT ACAGACCTTT CCTGCAAGCA 
     3121 AACTAAACAA ATTGGAGCAA CTGGAAGAAC TGAATCTAAG TGGGAACAAG CTTAAAGCTA 
     3181 TTCCCACAAC CATAGCAAAC TGCAAAAGAC TGCACACCCT TGTCGCACAT GCCAACAACA 
     3241 TCAGCATTTT CCCAGAGATC CTGCAGCTGC CTCAGATTCA GTTTGTTGAC CTGAGTTGCA 
     3301 ATGATTTGAC AGAAATACTA ATTCCAGAGA CTCTGCCTGC CACTTTACAA GACCTTGATC 
     3361 TTACAGGAAA TACAAATCTG GTTCTGGAAC ACAAGACTTT GGACATGTTT AGCCATATCA 
     3421 CAACACTGAA AATTGATCAG AAACCTTTAC CGACCACAGA TTCTACAGTT ACATCAACCT 
     3481 TCTGGAGCCA TGGACTGGCC GAGATGGCTG GGCAGAGGAA TAAACTCTGT GTGTCTGCTC 
     3541 TGGCTATGGA TAACTTTGCA GAGGGCGTGG GGGCTGTGTA CGGCATGTTT GATGGGGACA 
     3601 GGAATGAGGA ACTCCCCCGT CTGCTGCAGT GTACAATGGC AGACGTGCTT TTGGAAGAGG 
     3661 TACAGCACTC AACAAGTGAC ACCGTTTTCA TGGCCAATAC CTTCCTGGTG TCTCACAGGA 
     3721 AATTAGGAAT GGCTGGCCAG AAGCTAGGTT CCTCTGCTCT CCTGTGCTAT ATTCGACCTG 
     3781 ACACTGCCGA CCCAACAAGT AGTTTTAGCT TGACTGTAGC CAATGTTGGC ACGTGCCAAG 
     3841 CTGTCCTGTG CCGAGGTGGA AAGCCAGTAC CTCTTTCTAA AGTCTTCAGC TTGGAGCATG 
     3901 ACCCAGAGGA GGCACAGAGG GTGAAGGACC AGAAAGCTAT CATAACAGAG GACAACAAGG 
     3961 TGAATGGGGT TACCTGCTGC ACCCGGATGC TGGGATGTAC ATACCTCTAC CCCTGGATCC 
     4021 TCCCCAAGCC TCACATATCC TCCACTCCGC TTACCATTCA AGATGAATTG CTGATTTTGG 
     4081 GAAACAAAGC ACTGTGGGAA CACTTGTCCT ATCTAGAAGC TGTCAGTGCA GTCCGTCATG 
     4141 TGCAAGACCC ATTAGCTGCT GCTAAGAAGC TGTGCACATT AGCACAGAGC TATGGTTGTC 
     4201 AGGACAATGT GGGGGCAATG GTGGTTTATT TGAACATTGG TGAAGAAGGC TGTACCTGTG 
     4261 AAATGCATGG GCTTGCCCTT CCAGGCCCTG TGGGATTTGC TTCAACAGCA ACCATCAAGG 
     4321 ATGCCCCCAA GCCAACCACT CCCTCCTCTA GCAGTGGGAT TGCCTCAGAG CTGAGCAGTG 
     4381 AGGTGTCGAC CTCAGAGGTG AGCAGTGAGG TGGGTTCCAC AGCGTCTGAC GAACACAACA 
     4441 CTGTGGGCCT GGAGACGAGC TTGCTTCCCA GGCCTGAGAG GCGCTGTAGT CTGCACCCTG 
     4501 TGCCCTCTGC AGGCACTTTC CAGCGCCAGC CTTCTTGTGC TACCTTTTCC AGCAATCAGT 
     4561 CTGACAATGG ACTGGACAGT GACGATGACC AGCCTGTTGA AGGCGTCATA ACCAATGGCA 
     4621 GCAGGGTTGA GGTAGAGGTA GACATTCATT GCTGCCGAGG CAGAGAGTCT GAGAACTCCC 
     4681 CTACTCTCCC GAAGAATTCT TCCTACCCCT GTTCAGAGGA ACATGCTAGG GGGTTGGGTT 
     4741 TTGGGATCCG AAGACAGAAC AGTGTGAATA GTGGTATACT TCTGCCAGTG AACAAAGACA 
     4801 AGATGGAGTT ACAGAAGTCT CCCTCCACTT CTTGTTTGTA TGGAAAGAAA CTCTCCAATG 
     4861 GCTCCATTGT GCCCCTAGAA GACAGCCTGA ACCTCATTGA GGTGGCCACA GAAGCACCCA 
     4921 AGAGGAAAAC TGGCTATTTT GCTGCCCCCA CTCAGCTGGA ACCAGAGGAT CAATTTGTTG 
     4981 TCCCCCGTGA CCTGGAAGAC GAAGTGAAGG AGCAAATGAA GCAGCACCAG GAGAGCAGGC 
     5041 CTGAGCCTGA ACCCAGGGAA GAGGACCGGA CAGAGCCCCT GCAGGAGTTT GATACAGCAC 
     5101 TGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5161 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5221 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5281 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5341 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5401 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5461 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5521 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5581 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5641 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5701 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5761 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5821 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5881 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5941 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6001 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6061 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6121 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6181 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6241 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6301 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6361 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6421 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6481 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6541 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6601 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6661 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6721 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6781 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6841 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6901 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6961 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7021 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7081 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7141 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7201 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7261 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7321 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7381 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7441 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7501 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7561 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7621 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7681 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7741 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7801 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7861 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7921 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7981 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8041 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8101 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8161 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8221 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8281 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8341 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8401 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8461 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8521 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8581 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8641 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8701 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8761 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8821 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8881 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8941 TTTAACGCGA ATTTT
//