LOCUS       RR211163                7905 bp    DNA     circular  SYN 
DEFINITION  Egflam (Myc-DDK-tagged ORF) - Rat EGF-like, fibronectin type III and laminin G domains (Egflam), (10 ug)
ACCESSION   NM_001108938
VERSION     NM_001108938.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4060..4151 
                     /label="Myc-DDK"  
     misc_feature    4181..4766 
                     /label="PolyA signal"  
     misc_feature    4238..4257 
                     /label="Restriction Enzyme"  
     misc_feature    5042..5714 
                     /label="ColE1"  
     misc_feature    6144..6938 
                     /label="Kan/Neo"  
     misc_feature    6973..7275 
                     /label="SV40 ori"  
     misc_feature    7361..7838 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4035..4042 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4035 
                     /label="Egflam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTTAATC TCAACCTTCT TGCTCCATTT CTTGCTCCTG GCCTGCAGCC 
     1081 TCCCACCCGG CGCCGTGTCA CTCCGAACCG CTCTGCGAAA ATCAGGCAAG GTGGGACCTC 
     1141 CTCTTGACAT CAAACTGGGA GCCTTGAACT GTACAGCCTT CAGCATCCAG TGGAAAACAC 
     1201 CAAAGCGTTC TGGTAGCTCC ATTGTTGGGT ACACAGTATT TTACTCTGAG CTTGGTTCAG 
     1261 ATAAGTCCCT CCGGGAACAG TCACATAATG TACCTGTTGG CCAGGATACC CTAATCACTG 
     1321 AGGAAGTGAT TGGAGATTTG AAGCCAGGCA CTGAATATCG AGTGAGCATA GCAGCCTACA 
     1381 GCCAGACTGG CAAAGGGAGG CTTAGCTTCC CTCGGCATGT GACCACTTTG TCTCAAGATT 
     1441 CCTGCCTGCC CCCTGAAGCA CCCCATCAGC CACATGTCCT TGTGGTTTCT GATTCTGAAG 
     1501 TGGCCCTATC TTGGAGACCG GGAGAGAATG AAGGAAGTGC CCCAATTCAG TCCTACTCTG 
     1561 TGGAATTCAT TAGGCCAGAT TTTGACAAGA GCTGGACCAT AATCCAAGAG CGCCTGCAGA 
     1621 TGGACTCCAT GGTTATCAAA GGCCTTGATC CAGACACCAA CTACCAGTTT GCTGTGAGAG 
     1681 CCATGAATGC CTATGGCTTC AGTCTGCGCA GCCAGCCCAG CAACACCATC CGAACTCTGG 
     1741 GTCCTGGGGA GGCTGGAAGT GGCCGCTATG GACCTGGGTA TATCACCGAT ACCGGCGTGA 
     1801 GTGAGGATGA CGATGCATCA GAAGACGAGC TAGATCTGGA TGTCTCCTTT GAGGAGGTTA 
     1861 AACCACTTCC TGCTACCAAA GTTGGGAATA AGAAGTCCAA GAAGACTTCT GTCTCCAACT 
     1921 CAGAGATGGA CTCCAGACTT GCCCAACCTA CCTCAGCATC TCTCCCTGAA ACTACAGTGG 
     1981 CTGTGCCACC CACGCCAGCC CAACGGAAGG GGAAGAACAG TGTGGCCGTG ATGTCAAGGC 
     2041 TCTTTGACAT GTCCTGTGAC GAAACCCTCT GTTCAGCTGA CAGCTTCTGT GTCAATGACT 
     2101 ACGCCTGGGG AGGCTCACGA TGCCATTGCA ACCTGGGCAA AGGAGGCGAG GCCTGCTCAG 
     2161 AAGATATCTT TATTCAGTAT CCACAGTTCT TTGGCCACTC CTATGTAACC TTTGAGCCTC 
     2221 TGAAGAACTC TTATCAGGCA TTTCAGATTA CTCTGGAATT CAGGGCAGAG GCAGAAGATG 
     2281 GCTTACTGCT CTACTGTGGG GAGAGTGAAC ATGGGAGAGG AGACTTCATG TCCCTGGCTC 
     2341 TCATCCGACG CTCCCTCCAC TTCAGGTTTA ATTGTGGAAC TGGGATGGCC ATCATCATAA 
     2401 GTGAGACCAA AATCAAGCTT GGGGCCTGGC ACTCGGTGAC ACTGTATAGA GATGGGCTGA 
     2461 ACGGGCTGCT GCAGTTGAAC AATGGCACCC CAGTAACAGG CCAGTCCCAG GGTCAGTACA 
     2521 GTAAAATTAC CTTCCGGACA CCTCTGTACC TTGGTGGTGC TCCCAGTGCT TACTGGCTGG 
     2581 TCAGAGCGAC GGGGACAAAT CGTGGCTTCC AAGGCTGTGT GCAGTCACTT GCTGTTAACG 
     2641 GGAAGAAGAT TGACATGAGG CCCTGGCCAC TGGGGAAAGC CCTCAACGGA GCTGATGTTG 
     2701 GGGAGTGCAG CAGTGGGATC TGTGATGAAG CCTCCTGCAT CAACGGGGGC ACCTGCGCAG 
     2761 CCATCAAGGC CGACTCCTAC ATCTGCCTCT GTCCCCTTGG GTTCAGAGGT CGACACTGTG 
     2821 AAGATGCATT TACCCTGACC ATTCCTCAGT TCAGAGAGTC GCTGAGGTCC TACGCTGCCA 
     2881 CGCCCTGGCC CCTGGAGCCT CAGCACTATC TTTCTTTCAC CGAGTTTGAG ATCACATTTC 
     2941 GGCCAGACTC CGGGGATGGG GTCCTTTTGT ACAGCTACGA CACAAGCAGC AAGGACTTCC 
     3001 TGTCCATTAT TATGGCTGCA GGCCATGTGG AATTCCGCTT CGACTGTGGT TCTGGGACCG 
     3061 GAGTTCTCAG GAGTGAGGAT ACCCTCACCC TAGGCCAATG GCATGACCTA CGCGTGTCTC 
     3121 GCACAGCGAA GAATGGGATC TTACAGGTGG ACAAGCAGAA GGTAGTAGAA GGAATGGCAG 
     3181 AGGGAGGCTT CACCCAGATT AAGTGTAATA CAGACATTTT TATTGGCGGA GTCCCAAATT 
     3241 ACGATGATGT CAAGAAGAAC TCGGGTATCC TCCATCCATT TAGTGGGAGC ATCCAGAAGA 
     3301 TCATCCTAAA TGACCGGACC ATCCATGTGA GGCACGACTT TACATCTGGT GTGAATGTGG 
     3361 AGAACGCAGC GCACCCCTGT GTGGGGGCCC CCTGTGCCCA TGGGGGCAGC TGTAGGCCCA 
     3421 GGAAGGAGGG TTATGAGTGT GACTGTCCCT TGGGCTTCGA AGGCCTGAAC TGCCAGAAAG 
     3481 CGATTACAGA AGCCATTGAG ATCCCACAGT TCATCGGTCG GAGTTACCTG ACCTATGACA 
     3541 ACCCAAATAT CCTCAAGAGG GTGTCCGGAT CAAGGTCAAA TGCTTTCATG AGGTTTAAGA 
     3601 CAACCGCCAA GGACGGCCTG TTGCTGTGGA GGGGGGACAG CCCCATGAGA CCTAACAGCG 
     3661 ACTTCATTTC CCTGGGTCTT CGGGATGGAG CCCTGGTGTT CAGCTACAAC CTGGGCAGTG 
     3721 GTGTGGCTTC CATTATGGTG AATGGTTCCT TCAGCGATGG CCGGTGGCAC CGAGTCAAGG 
     3781 CTGTCAGGGA TGGCCAGTCT GGGAAAATTA CGGTGGATGA CTATGGAGCC AGAACAGGCA 
     3841 AGTCACCTGG CATGATGAGG CAACTTAACA TCAATGGGGC TCTGTATGTG GGTGGAATGA 
     3901 AGGAAATTGC TCTGCGCACG AACAGACAGT ACATGCGAGG CCTTGTGGGC TGCATCTCCC 
     3961 ATTTCACATT GTCCACCGAT TACCACATTT CCCTTGTGGA AGACGCTGTG GATGGGAAGA 
     4021 ACATCAACAC TTGTGGGGCT AAGAGCGGAC CGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4081 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4141 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4201 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4261 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4321 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4381 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4441 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4501 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4561 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4621 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4681 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4741 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4801 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4861 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4921 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4981 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5041 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5101 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5161 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5221 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5281 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5341 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5401 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5461 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5521 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5581 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5641 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5701 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5761 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5821 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5881 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5941 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6001 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6061 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6121 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6181 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6241 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6301 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6361 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6421 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6481 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6541 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6601 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6661 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6721 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6781 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6841 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6901 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6961 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7021 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7081 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7141 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7201 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7261 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7321 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7381 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7441 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7501 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7561 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7621 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7681 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7741 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7801 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7861 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//