LOCUS       RR211064                8649 bp    DNA     circular  SYN 
DEFINITION  Stag3 (Myc-DDK-tagged ORF) - Rat stromal antigen 3 (Stag3), (10 ug)
ACCESSION   NM_053730
VERSION     NM_053730.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4806..4897 
                     /label="Myc-DDK"  
     misc_feature    4927..5512 
                     /label="PolyA signal"  
     misc_feature    4984..5003 
                     /label="Restriction Enzyme"  
     misc_feature    5788..6460 
                     /label="ColE1"  
     misc_feature    6890..7684 
                     /label="Kan/Neo"  
     misc_feature    7719..8021 
                     /label="SV40 ori"  
     misc_feature    8107..8584 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4788..4794 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4788 
                     /label="Stag3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTACTCTG TGGTCACCCT CCACCCAGCA CCATGGCTCT TCCTCAGGCA 
     1081 GTATGTCCTC CCCTCTTCGA AAGTCTGTGA GATGTGCACA GATGGCCTTG TCTCCTTGTT 
     1141 CTTCCAACAT CCAACCCTGT GATGACAGAG ACTCCCAGGG AACTGCAGAA TGGGATAGTT 
     1201 CCTCAACTAG TGAAGACAGT GACTTTGAAG ATAGCTTAAG AAGAAATGTG AGGAAGAGAG 
     1261 CAGCAAAACG ACCACCCAAA GCTATCCCAG TGGCAAAACA TCCGAAGAAG CAGTCCCACA 
     1321 TAGTACCTGG TGGGAATGAC AAGAACAAGT CAGTGCCGCC AACCAGTGAC CTTTTTGATG 
     1381 CTGTGAAAGC TGCTAGAAGT TGTGCGCAGT CTTTGGTAGA TGAGTGGCTA GAAAACTACA 
     1441 AGCAAGATGA AAATGCAGGA TTCTTGGAAC TTGTTAATTT TTTCATCCGA GCCTGTGGAT 
     1501 GTAAAAGCAC TGTCACACCC GAGATGTTCA AGACAATGTC CAACTCAGAG ATCATCCAGC 
     1561 ACCTAACAGA AGAGTTTAAT GAGGACTCAG GTGACTATCC CCTGACAGCT CCAGGTCCAT 
     1621 CCTGGAAGAA GTTCCAGGGA AGCTTCTGTG AGTTTGTGAA GACACTAGTC TGTCAGTGCC 
     1681 AGTACAGCCT CCTCTTTGAC GGCTTTCCAA TGGATGACCT TATCTCCCTG CTCATTGGCC 
     1741 TCTCAGATTC CCAGGTCCGA GCCTTTCGTC ATACTAGTAC TTTGGCTGCC ATGAAGCTAA 
     1801 TGACTTCTCT GGTAAAAGTT GCACTCCAGT TGAGTCTGCA CAAAGACAAC AATCAACGTC 
     1861 AGTATGAGGC AGAACGAAAC AAGGGGCCAG AGCAGAGGGC ACCAGAGCGG CTCGAGAGTC 
     1921 TGCTGGAGAA ACGAAAAGAG TTCCAAGAGA ATCAAGAGGA GATAGAGGGG ATGATGAATG 
     1981 CCATCTTCAG GGGTGTCTTT GTTCATCGGT ACAGGGACAT CCTTCCTGAG ATCCGTGCTG 
     2041 TCTGCATCGA GGAGATCGGG TGTTGGATGC AAAGCTACAG CACCTCCTTT CTTAATGACA 
     2101 GCTACCTAAA ATATATTGGC TGGACCCTGC ATGACAAGCA CAAGGAAGTC CGCCTAAAGT 
     2161 GTGTGAAGGC TCTGGCAGGG CTGTACAGCA ACCAGGAGCT GAGTTCACGG ATGGAGCTCT 
     2221 TTACTAATCG CTTCAAGGAC CGGATGGTTT CCATGGTCAT GGACAGAGAG AGTGAAGTAG 
     2281 CAGTGGAGGC CATCAGATTG CTGACCCTTA TTCTGAAGAA CATGGAGGGA GTACTGACTA 
     2341 GTGCAGATTG TGAGAAAATT TACTCCATTG TATACATTTC TAATCGTGCC ATGGCCTCTT 
     2401 CTGCAGGGGA ATTTGTGTAC TGGAAGATTT TCCATCCTGA ATGTGGGGCA AAAGCAGTGA 
     2461 GTGGCAGGGA GCGACGCCGG AGTCCACAAG CCCAGAGGAC TTTCATTTAC CTTTTATTGG 
     2521 CCTTCTTTAT GGAGAGTGAG CATCACGACC ATGCTGCTTA CCTAGTTGAC AGCTTGTGGG 
     2581 ACTGTGCAGG GTCTTACCTG AAGGACTGGG AGAGTCTGAC AAGTCTGTTG CTGCAGAAAG 
     2641 ACCAGAATCT GGGTGATATG CAAGAGAGAA TGTTGATAGA AATCCTGGTG TCCAGTGCCC 
     2701 GGCAAGCTGC AGAGGGTCAC CCACCAGTGG GGCGCATCAC TGGAAAGAAG AGTCTGACCG 
     2761 CCAAAGAACG CAAGCTTCAA GCTTATGATA AGGTGAAGCT GGCTGAGCAC CTCATCCCCC 
     2821 TCTTGCCCCA GCTCCTTGCC AAGTTCTCAG CAGATGCAGA GAACGTTGCT CCCTTGCTCC 
     2881 GGCTGCTCAG TTACTTTGAC CTCAACATTT ATTGCACTCA GCGCTTGGAG AAGCACTTGG 
     2941 AGCTGCTTCT GCAACAACTC CAGGAGGTGG TGGTGAAGCA CGTAGAGCCT GAGGTGCTTG 
     3001 AGGCTGCAGC ACATGCCCTC TATTTGCTCT GCAAGCCAGA GTTCACCTTC TTCAGCAGAG 
     3061 TGGACTTCGC CAGAAGCCAA TTAGTAGATC TGCTGACTGA TAGATTCCAG CAGGAGCTTG 
     3121 ACGACCTAAT GCAGTCATCC TTCCTAGATG AGGATGAGGT ATACAGTCTG ACAGCCACTC 
     3181 TGAAGCGTCT CTCTGCCTTT TACAATGCTC ATGACCTGAC TCGCTGGGAG ATCTCTGAAC 
     3241 CATGTTCTCG ACTCCTCCGG AAGGCTGTAG ACACAGGAGA AGTTCCTCAC CAGGTGATTT 
     3301 TGCCAGCCTT GACTCTGGTA TATTTTTCCA TTCTCTGGAC AGTGACCCAC ATTTCAGAGT 
     3361 CTACTTCCCA AAAGCAGCTG ATGAGTCTGA AGAAAAGAAT GGTAGCCTTC TGTGAACTTT 
     3421 GCCAAAGCTG CCTCTCAGAC GTGGACCCAG AGATCCAGGA GCAGGCTTTT GTTTTATTAA 
     3481 GTGACCTGCT TCTCATCTTC AGCCCCCAGA TGGTTGTAGG GGGCCGGGAT TTCCTTAGGC 
     3541 CTCTTGTCTT TTTTCCGGAA GCTACTCTCC AGTCGGAACT AGCCAGCTTC CTCATGGACC 
     3601 ATGTCTTTCT CCAGCCTGGA GAATTGGGCA ACGGTCAATC ACAGGAGGAT CACGTCCAAA 
     3661 TAGAGCTTCT GCACCAGAGG CGCCGCCTGC TTGCAGGATT TTGTAAGCTG TTGCTTTATG 
     3721 GGGTATTGGA ACTGGATGCG GCCTCAGATG TTTTCAAACA CTACAACAAG TTCTATGAAG 
     3781 ATTATGGTGA CATTATCAAG GAAACATTAA CTCGGGCGAG ACAAATTGAC CGATGTCAGT 
     3841 GCTCTCGGAT CCTGCTCCTG AGCCTAAAGC AGCTCTACAC AGAACTGATA CAGGAGCAGG 
     3901 GGCCCCAGGA CCTGACAGAA CTGCCAGCCT TCATTGAGAT GAGAGACCTG GCCCGGAGGT 
     3961 TTGCCTTGAG CTTTGGACCC CAGCAGCTCC ATAACAGAGA TCTTGTGGTC ATGCTGCACA 
     4021 AGGAAGGCAT CAAGTTCTCA TTGTCTGAGC TTCCTCCTGC TGGCTCTTCT CGAGAGCCCC 
     4081 CAAATATTGC ATTCCTGGAG CTTCTTTCAG AGTTCTCCCC CCGCCTCTTC CATCAGGACA 
     4141 AACAGCTACT ACTATCATAC CTGGAAAAGT GTCTGCAGCG TGTCTCCATG GCACCTAGCC 
     4201 ATCCCTGGGG TCCAGTCACC ACCTACTGCC ACTCCCTCCA TCTAGTAGAG AACACAGCAG 
     4261 AGGCCAGCTC TCAGGGGCCC CCCCACTCCA AGAAGAGGTG TATTGAAGTT CCCCGCAGGC 
     4321 TTCAGGAAGA AGAGTCTTCA TCCCAGGGAG AAAGCCTTCA GCTGAACAGT GGCCCCACAA 
     4381 CACCTACACT CACCTCCACA GCAGTGAAGA GAAGGCAGTC TCCGAGGACA GTAGGCAAGA 
     4441 GGCAAAAAGG TGGACCAGGA CCAGGACCAG GACCAGGACC AGGACCAGGA CCAGGACCAG 
     4501 GACCAGGACC AGGACCAGGA CCAGGACCAG AGCTGATCTG CAGTCAGCAG CTCTCAGGCA 
     4561 CCCAGAGGTT GAAAATGTCG AGTGCACCGT GTTTCCAGAT TCGATGTGAT CCTTCTGGCT 
     4621 CTGGCTTGGG CAAGCAGATG ACCCGACTCA GCCTTATGGA AGAAGATGAG GAAGAAGAGC 
     4681 TGAGACTTCT GGATGAAGAA TGGCAATGTG GAGACAAGCT ACTTCATAGC CCTTCTTCTC 
     4741 CCAGTGAGCA TGGGCTGGAC CTATTAGATA CAACAGAGCT GAACATGGAG GATTTCACGC 
     4801 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4861 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4921 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4981 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5041 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5101 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5161 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5221 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5281 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5341 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5401 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5461 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5521 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5581 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5641 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5701 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5761 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5821 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5881 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5941 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6001 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6061 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6121 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6181 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6241 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6301 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6361 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6421 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6481 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6541 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6601 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6661 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6721 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6781 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6841 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6901 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6961 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7021 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7081 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7141 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7201 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7261 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7321 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7381 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7441 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7501 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7561 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7621 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7681 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7741 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7801 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7861 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7981 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8041 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8161 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8221 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8281 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8341 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8401 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8461 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8521 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8581 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8641 GCGAATTTT
//