LOCUS       RR211007                6819 bp    DNA     circular  SYN 
DEFINITION  Hspa8 (Myc-DDK-tagged ORF) - Rat heat shock protein 8 (Hspa8), (10 ug)
ACCESSION   NM_024351
VERSION     NM_024351.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2976..3067 
                     /label="Myc-DDK"  
     misc_feature    3097..3682 
                     /label="PolyA signal"  
     misc_feature    3154..3173 
                     /label="Restriction Enzyme"  
     misc_feature    3958..4630 
                     /label="ColE1"  
     misc_feature    5060..5854 
                     /label="Kan/Neo"  
     misc_feature    5889..6191 
                     /label="SV40 ori"  
     misc_feature    6277..6754 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2958..2964 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2958 
                     /label="Hspa8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTAAGGGA CCTGCAGTTG GCATTGATCT TGGCACCACC TACTCCTGTG 
     1081 TGGGTGTCTT CCAGCATGGA AAGGTGGAAA TAATTGCCAA TGACCAGGGT AACCGCACCA 
     1141 CGCCGAGCTA TGTTGCTTTC ACCGACACAG AACGATTAAT TGGGGATGCG GCCAAGAATC 
     1201 AGGTTGCAAT GAACCCCACC AACACAGTTT TTGATGCCAA ACGTCTGATC GGACGTAGGT 
     1261 TCGATGATGC TGTTGTTCAG TCTGACATGA AGCACTGGCC CTTCATGGTG GTGAACGATG 
     1321 CAGGCAGGCC CAAGGTCCAA GTCGAATACA AAGGGGAGAC AAAAAGTTTC TATCCTGAGG 
     1381 AAGTGTCTTC AATGGTTCTG ACAAAAATGA AGGAAATTGC AGAAGCTTAC CTTGGAAAGA 
     1441 CTGTTACCAA TGCCGTGGTC ACCGTGCCAG CTTACTTCAA TGACTCTCAG CGACAGGCAA 
     1501 CAAAAGATGC TGGAACTATT GCTGGCCTCA ACGTACTTCG AATTATCAAT GAGCCAACTG 
     1561 CTGCTGCTAT TGCCTATGGC TTAGATAAGA AGGTCGGGGC TGAAAGGAAT GTGCTCATTT 
     1621 TTGACTTGGG AGGTGGCACT TTTGATGTGT CAATCCTCAC TATCGAGGAT GGAATTTTTG 
     1681 AAGTCAAATC AACAGCTGGA GACACCCACT TGGGCGGAGA AGACTTTGAC AACCGAATGG 
     1741 TCAACCATTT CATTGCTGAG TTTAAGCGAA AGCACAAGAA GGACATCAGT GAGAACAAGA 
     1801 GAGCTGTCAG GCGTCTCCGC ACTGCCTGTG AGCGGGCCAA GCGCACCCTC TCCTCCAGCA 
     1861 CCCAGGCCAG TATTGAGATT GATTCTCTCT ATGAGGGAAT TGACTTCTAC ACCTCCATTA 
     1921 CCCGTGCTCG ATTTGAGGAG TTGAATGCTG ACCTGTTCCG TGGCACACTG GACCCTGTAG 
     1981 AGAAGGCCCT TCGAGATGCC AAACTAGACA AGTCACAGAT CCATGATATT GTCCTGGTGG 
     2041 GTGGTTCTAC CAGAATCCCC AAGATCCAGA AACTTCTGCA AGACTTCTTC AATGGAAAAG 
     2101 AGCTGAATAA GAGCATTAAC CCCGATGAAG CTGTTGCCTA TGGTGCAGCT GTCCAGGCAG 
     2161 CCATTCTATC TGGAGACAAG TCTGAGAATG TTCAGGATTT GCTGCTCTTG GATGTCACTC 
     2221 CTCTTTCCCT TGGGATTGAA ACTGCTGGTG GAGTCATGAC TGTCCTCATC AAGCGCAATA 
     2281 CCACCATTCC CACCAAGCAG ACCCAGACTT TCACCACCTA CTCTGACAAC CAGCCAGGTG 
     2341 TACTCATCCA GGTGTATGAA GGTGAAAGGG CCATGACCAA GGACAACAAC CTGCTTGGGA 
     2401 AGTTTGAGCT CACAGGCATA CCTCCAGCAC CCCGTGGGGT TCCTCAGATT GAGGTTACTT 
     2461 TTGACATTGA TGCCAATGGC ATCCTCAATG TTTCTGCTGT AGATAAGAGC ACAGGAAAGG 
     2521 AGAACAAGAT CACCATCACC AATGACAAGG GCCGCTTGAG TAAGGAGGAT ATTGAGCGCA 
     2581 TGGTCCAAGA AGCTGAGAAG TACAAAGCTG AGGATGAGAA GCAGAGAGAT AAGGTTTCCT 
     2641 CTAAGAACTC GCTGGAGTCT TATGCTTTCA ACATGAAAGC AACTGTTGAG GATGAGAAAC 
     2701 TTCAAGGCAA GATCAATGAT GAAGACAAAC AGAAGATTCT TGACAAGTGC AACGAAATCA 
     2761 TCAGCTGGCT GGATAAGAAC CAGACTGCGG AGAAGGAAGA ATTTGAGCAT CAGCAGAAAG 
     2821 AACTGGAGAA GGTCTGCAAC CCTATCATCA CCAAGCTGTA CCAGAGTGCT GGTGGCATGC 
     2881 CTGGAGGAAT GCCTGGTGGC TTCCCTGGTG GAGGAGCTCC TCCATCTGGT GGTGCTTCTT 
     2941 CAGGCCCCAC CATTGAAGAG GTCGATACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3001 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3061 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3121 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3181 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3241 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3301 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3361 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3421 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3481 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3541 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     3601 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     3661 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     3721 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     3781 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     3841 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     3901 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     3961 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4021 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4081 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4141 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4201 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4261 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4321 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4381 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4441 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4501 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4561 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     4621 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     4681 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     4741 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     4801 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     4861 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     4921 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     4981 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5041 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5101 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5161 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5221 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5281 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5341 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5401 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5461 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5521 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     5581 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     5641 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     5701 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     5761 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     5821 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     5881 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     5941 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6001 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6061 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6121 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6181 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6241 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6301 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6361 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6421 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6481 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6541 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     6601 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     6661 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     6721 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     6781 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//