LOCUS       RR210985                9234 bp    DNA     circular  SYN 
DEFINITION  Fyco1 (Myc-DDK-tagged ORF) - Rat FYVE and coiled-coil domain containing 1 (Fyco1), (10 ug)
ACCESSION   NM_001106870
VERSION     NM_001106870.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5391..5482 
                     /label="Myc-DDK"  
     misc_feature    5512..6097 
                     /label="PolyA signal"  
     misc_feature    5569..5588 
                     /label="Restriction Enzyme"  
     misc_feature    6373..7045 
                     /label="ColE1"  
     misc_feature    7475..8269 
                     /label="Kan/Neo"  
     misc_feature    8304..8606 
                     /label="SV40 ori"  
     misc_feature    8692..9169 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5373..5379 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5373 
                     /label="Fyco1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCTAGC AGCACTGAGA CGCAGCTCCA GAGGATCATC CGTGATCTAC 
     1081 AAGATGCAGT GACAGAACTA AGCAATGAAT TTAAGGAAGG CGGAGAACCA ATCACAGATG 
     1141 ACAGCACCAG CTTGCACAAG TTTTCTTACA AACTTGAGTA TCTTCTGCAA TTTGATCAGA 
     1201 AGGAGAAGGC TAGCCTCCTG GGTAGCAAGA AGGACTACTG GGATTACTTC TGTGCGTGCC 
     1261 TAGCCAAGGT GAAAGGAGCC AATGATGGGA TTCGATTCGT CAGGTCCATC TCTGAGCTCC 
     1321 GAACATCTCT AGGAAAGGGG AGAGCCTTCA TTCGCTACTC TTTGGTGCAC CAGAGGTTGG 
     1381 CAGACACCTT GCAGCAGTGC TTCATGAACA CCAAAGTGAC CAGTGACTGG TATTATGCAA 
     1441 GAAGCCCCTT TCTGAAGCCA AAGCTGAGCT CTGACATTGT GGGCCAGCTG TATGAGCTGA 
     1501 CTGAGGTTCA GTTTGATCTA GCATCAAGAG GCTACGACTT GGATGCCGCC TGGCCAACAT 
     1561 TTGCCAGGAG GACGCTAGCC ACCAGCTCTT CTTACATGTG GAAACCCCCC AGCCGAAGCT 
     1621 CCAGCATGAG TAGTTTAGTG AGCAACTACT TGCAGACTCA GGAGATGGCC TCCAGTCTTG 
     1681 ACCTGAATAG TTCTCTAAAC AATGAAGCAC TAGAAAGCTT CGATGAGATG CGGCTGGAGC 
     1741 TGGACCAGTT GGAGGTTCGG GAGAAACAGC TACAAGAGCA CGTGCAGCAG CTAAACAGGG 
     1801 AAAACCAAGC ACTTCGAATG ACGATCAGCA AGCAAGGGGG GCAACTTCAG GTAGAGAAGG 
     1861 AAACGGGGTA CCTTGCAGTC GAGGATAGCA TTGACCTCGT GAGCCTGGTG GCAGAGCTCC 
     1921 AGAAGCAGGG GGAGGTCACC CAGGCCACAG TGAAGAAGCT ACAGACATGT CTACAGGCTC 
     1981 TGGAGTTAGC TGTAGACCAG AAAGAGACCA GCCACTCAGT CTTGCAGCTT GAGAACGTGG 
     2041 CACAGGCACT TGACGCTGTG AGAGACTCCT TGGGTAGGAA GAACCAGCTT CTAGCCAGCC 
     2101 TTTCTGAATG CCTCACTAGG GCTGAAAAAA GGGAACATCC ATCTCTGGAC ACAAAGCTTC 
     2161 ATCAAGAGCC AGTTCCTACT GACCTGGCCC TGAAGTTCCA GGAGCTGAAG GGAAAACTTC 
     2221 AAACCCTAGA AGGAGAGAAC ACCAAGGCCC AGGAGCTAAA CAGGCAGCAA AGTCTCAAGC 
     2281 TAGAGCAGCT GGCCAAGGAG CTGCAGCTGA AGGAGGAAGC CCGAGCTAGC CTGGAACACT 
     2341 TAGTGAAGGA TGCGGTCCCA CTTCAGGAAG AGCTATCCGG AAAGAAGCAA GAGTCAGCTC 
     2401 AGCTCCGACG ACAGCTTCAG GAATCCCTGG CCCACTTGAG CTCTGTGGAG GAGGAGCTGG 
     2461 CTGAAGCCAG ACAGCAGGAA AAGCAGCATC GAGAAGAAAA ACAGCTACTA GAGCAGGAGG 
     2521 CCAAGTCTCT GACATGGCAG CTGCAGCTCC TAGAGACCCA ACTAGGACAG GTGAGTCAGC 
     2581 TTGTGAGTGA CCTGGAGGAG CAGAAGAAGC AACTCATGCA GGACAAAGAC CATCTCAGCC 
     2641 AGAAAGTAGG AACACTAGAG CAGCTAGCTG AGGCCCATGG GGCAAGTAAG AGTAAAGAGG 
     2701 CTGTCCCACC ACAGGCTGGG GAGATGCCAG AAAAGGGGCA GCACTGCCTC CAGGAAGAAC 
     2761 AGGTAAACAA TGCCACAGTG CGAGGGGCAG ACCAGGACGA GTTACAAAAG GTCAACCAGG 
     2821 AGCTACAGAA GGAGCTGCAG AACATGGCTG TGCGCAACCA GCTCCTAGAA GGCAAACTGC 
     2881 AGGCCTTGCA AACTGATTAC AAGGCACTGC AGCAGCGAGA GGCAGCAATC CAGGGCTCTT 
     2941 TAGCCTCCCT GGAAGCTGAG CAGGCCAGCA TCCGGCAGCT GGGAAACCAG ATGGAAGCAA 
     3001 GCCTCCTGGC TGTGAGGAAA GCCAAAGAGA CTATGAAGGC CCAGGTGGCA GAGAAGGAGG 
     3061 CTGCCCTGCA GAGTAAGGAG GGCGAGTGCC GGCAGCTGCA GGAAGAGGCA GAGCAGTGCC 
     3121 GGCTTCGGAC AGAGGCCCAG GCCCAGGAGC TCAGAGCTCT TGAGAACCAG TGCCAGCAGC 
     3181 AGATTCAACT GATTGAGGTC CTCTCTGCAG AAAAAGGCCA ACAGGGACTC AGCCTACCCC 
     3241 AGGTCAACAC AGCCCAACTG GCCCTGTCTC AAGCACAGCT TGAAATTCAC CAGGGGGAAG 
     3301 CCCAGCGGTT ACAGAATGAG GTGGTGGACC TCCAGGCCAA GCTCCAGGTG GCCCTAGGTG 
     3361 ATCGAGACAA GTTACAGAGC CAACTGGGTG TGGCTGAGGC AGTTCTCGGG GAGCACAAGA 
     3421 CCCTGGTGCA ACAACTGAAA GAACAGAATG AAGCCCTCAA CAGGGCCCAT ATTCAGGAGC 
     3481 TGCTGCAGTG CTCAGAGAGA GAGGGGATAC TGCAGGAGGA GAGCATCTAC AAGGCCCAGA 
     3541 AGCAGGAGCA AGAGCTACGA GCCCTGCAGG CAGAGCTGTC ACAGGTGAGG TGCAGCTCTG 
     3601 AGGGAGCCCA CCTGGAGCAT GCAGAGCTAC AGGACCAGCT GCACCGAGCC AACACAGACA 
     3661 CAGCTGAGCT AGGCATACAG GTCTGTGCAC TGACAGCTGA GAAGGATCGG ATGGAGGGGG 
     3721 CCCTGGCCAG CCTAGCCCAG GAGCTCCAGG ACTCCAAAGA AGCAGCACTA CAGGAACGAA 
     3781 AGGGCTTAGA GCTCCAAGTG ATGCAACTTC AGCAAGAGAA GGAGAGCTTG CAGGAAAAGC 
     3841 TGAAGGCAAC TGAGGAGGCA GCCAGTTCAT TCTCTGTTCT TCAGGCACAG CTGGCCCAGG 
     3901 CTGAGCAGCT GGCCCAGAGC CTCCAAGAGG CTGCACACCA GGAACAAGAT GCCCTTAAGT 
     3961 TCCAGCTAAG TGCTGAGATT ATGGACCACC AGAACAGACT AAAGACAGCC AATGAAGAGT 
     4021 GTGGGAGCCT CAGGGCCCAG CTAGAAGAAC AAGGCCAGGA GCTGCAAATG ACTAAGGAGG 
     4081 CTGTGCAGGA ACTGAAGATC ACCAAGGCGA CCATGGAGGA GAAGCTGAAT TGCACCGGTA 
     4141 GCCACCTTGC AGAGTGCCAG GCCACTTTGC TACGCAAAGA TGAAGAAAGC GCTATGCTTC 
     4201 GAATCAACCT AGAGAGAACA CAGAAGGAAC TTGAAAAGGC TACATCAAAA TTCCAAGAAT 
     4261 ATTACAACAA ACTCTGCCAG GAGGTGACAA ACAGGGAAAG GAATGACCAG AAGATGCTTG 
     4321 CAGACCTAGA TGACCTGAAC AGAACCAAGA AATACCTTGA GGAGCGGCTG ATAGAGCTGC 
     4381 TCAGGGACAA AGATGCTCTC TGGCAAAAGT CCGATGCACT GGAATTCCAG CAGAAGCTCA 
     4441 GTGCTGAAGA GAAATGTCTT GGGGACATGG AGGCCAACCA CTGCCATGAC TGCAAGCGGG 
     4501 AGTTCAGCTG GATAGTGCGG CGGCACCACT GCAGGTTATG TGGCCGAATA TTCTGTTACT 
     4561 ACTGCTGTAA CAACTATGTT GTGACTAAGC CCAGTGGCAA GAAGGAACGG TGCTGCCGAG 
     4621 CCTGCTTCCA GAAGTTTGGT GAAAGCTCTG GATCCCCTGA CAGCAGTGGT TCGGGCACTA 
     4681 GCCAGGGAGA GCCCAGCCCC ATGGTGTCAC CAGCTGAAGC AGCTCCCCAG TCCATAGGAA 
     4741 GCCAAGGAAT AAACCCAGTC TGCAGACCAC CAGATGATGC TGTGTTTGAC ATCATCACCG 
     4801 ATGAGGAACT GTGCCAGATC CAAGAATCTG GCTCCTCCTT GCCTGAAACA CCCACCGAAA 
     4861 CTGATTCGAT GGACCCAAAT ATGGCTGAAC AGGACACCAC ATCAAACTCA TTAACCCCTG 
     4921 AAGACACTGA AGACATGCCC CTGGGGCAGG ATGCTGAAAT CTGCTTGCTG AAGTCAGGAG 
     4981 AGCTGATGAT CAAATTACCC CTCACAGTAG AGGAGATCGC CAGCTTTGGG GAGGGAAACA 
     5041 GGGAGCTGTT TGTGAGGTCC AGTACCTACA GCCTGATCCC CATCACCGTG GCTGAGCCCG 
     5101 GCCTTACCAT CAGCTGGGTC TTCTCTTCTG ATCCCAAGAG CATCTCCTTC AGTGTGGTCT 
     5161 TCCAAGAGAC AGAAGACACG CCACTCGACC AGTGCAAGGT CCTCATTCCC ACCACACGCT 
     5221 GCAATTCCCA CAAGGAGAGC ATCCGGGGCC AGCTGAAGGT CCGAACCCCT GGCATCTACT 
     5281 TGCTCATCTT TGACAACACC TTTTCCAGAT TTATCTCCAA AAAAGTATTT TACCACCTGA 
     5341 CCGTTGACCG GCCTGTGATC TACGATGGAA GTGATTTCCC AACGCGTACG CGGCCGCTCG 
     5401 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5461 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5521 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5581 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5641 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5701 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5761 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5821 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5881 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5941 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     6001 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     6061 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6121 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6181 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6241 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6301 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6361 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6421 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6481 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6541 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6601 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6661 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6721 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6781 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6841 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6901 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6961 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     7021 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     7081 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7141 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7201 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7261 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7321 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7381 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7441 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7501 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7561 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7621 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7681 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7741 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7801 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7861 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7921 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7981 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     8041 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     8101 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8161 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8221 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8281 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8341 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8401 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8461 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8521 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8581 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8641 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8701 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8761 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8821 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8881 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8941 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     9001 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     9061 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9121 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9181 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//