LOCUS       RR210889                8256 bp    DNA     circular  SYN 
DEFINITION  Tert (Myc-DDK-tagged ORF) - Rat telomerase reverse transcriptase (Tert), (10 ug)
ACCESSION   NM_053423
VERSION     NM_053423.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4413..4504 
                     /label="Myc-DDK"  
     misc_feature    4534..5119 
                     /label="PolyA signal"  
     misc_feature    4591..4610 
                     /label="Restriction Enzyme"  
     misc_feature    5395..6067 
                     /label="ColE1"  
     misc_feature    6497..7291 
                     /label="Kan/Neo"  
     misc_feature    7326..7628 
                     /label="SV40 ori"  
     misc_feature    7714..8191 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4395..4401 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4395 
                     /label="Tert"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGCGCT CCTCGTTGCC CCGCCGTGCG CTCTCTACTG CGCAGCCGAT 
     1081 ATCGGGAGGT GTGGCCGCTG GCGACCTTTG TGCGGCGCCT GGGGCTTGAG GGCAGTCGGC 
     1141 TTGTGCAACC CGGGGACCCG AAGGTCTTCC GCACGTTGGT TGCCCAGTGC CTAGTGTGCG 
     1201 TGCCCTGGGG CTCACAGCCG CCACCTGCTG ACCTTTCCTT CCACCAGGTG TCATCCCTGA 
     1261 AAGAGCTGGT GTCCAGGGTT GTGCAGAAAC TTTGCGAGCG CGGTGAGAGG AATGTGCTGG 
     1321 CTTTTGGCTT TGCACTGCTT AACGGGGCCA GAGGTGGGCC TCCCATGGCC TTCACGACCA 
     1381 GCGTGCATAG CTACTTGCCC AACTCGGTTA CTGAGTCCCT GTGTGTCAGT GGTGCATGGA 
     1441 TGCTACTGTT GAGCCGAGTG GGCGACGACC TGCTGGTCTA CCTGCTGTCG CACTGTGCGC 
     1501 TCTACCTGCT GGTGCCCCCC AGCTGTGCCT ACCAGGTGTG CGGGTCACCC CTGTACCAAA 
     1561 TTTGTGCCAC CACGGATACC TGGTCCTCTG TGCCCGCTGG TTACAGGCCC ACTCGACCCG 
     1621 TGGGCGGGAA TTTCACTAAC CTTGGGTCCG CACACCAGAT CAAAAACAGT GGTCACCAGG 
     1681 AAGCACCAAA ACCCCAGGCC TTGCCATCAC GAGGTACGAA GAGGCTTCTG AGTCTCACCA 
     1741 GTACAAACGT GCCTTCAGCT AAGAAGGCCA GGTTTGAACC TGCCCTGAGA GTGGATAAGG 
     1801 GACCCCACAG GCAGGTGGTA CCAACCCCAT CAGGCAAAAC ATGGGCGCCA AGTCCTGCTG 
     1861 CGTCCCCCAA GGTGCCTCCT GCAGCGAAAA ACTTGTCTTT GAAAGGAAAG GCATCTGACC 
     1921 CGAGTCTCTC TGGGTCGGTG TGCTGTAAAC ACAAGCCCAG CTCCTCGTCC CTGCTGTCAT 
     1981 CACCACCCCA AGATGCTGAA AAGCTCAGGC CATTCACTGA GACCAGACAT TTCCTTTACT 
     2041 CCAGGGGAGG TGGCCAAGAG GAGCTAAATC CCTCATTCCT ACTCAACAGC CTCCCGCCTA 
     2101 GCTTGACCGG GGCCAGGAGA CTGGTGGAGA TCATCTTTCT GGGCTCAAGG CCTAGGACAT 
     2161 CAGGACCATT CTGCAGGACC CGCCGCCTGC CCCGTCGATA CTGGCAGATG CGACCCCTAT 
     2221 TCCAGCAGCT GCTCATGAAC CACGCAAAGT GCCAATATGT CAGATTCCTC CGGTCGCACT 
     2281 GCAGATTTCG AACAGCAAAC CAGCGGGTGC CGGATGCCAT GGACACCAGC CCATCCCACC 
     2341 TCACGAGTTT GCTCCGGTTA CACAGCAGCC CCTGGCAGGT ATACGGCTTT CTTCGGGCCT 
     2401 GCCTCCGCGA GCTGGTGCCT GCCGGTCTCT GGGGCACCAG GCACAATGAG CGCCGCTTCT 
     2461 TAAAGAACGT GAAGAAGTTC ATCTCGTTGG GGAAGTACGC CAAGCTATCC CTGCAGGAAC 
     2521 TGATGTGGAG GGTGAAAGTG GAGGACTGCC ACTGGCTCCG CAGCAGCCCA GAGAAGGACA 
     2581 CTGTCCCTGC CGCAGAGCAC CGTCTGAGGG AGAGGATCCT TGCCATGTTC CTGTTCTGGC 
     2641 TAATGGACAC ATATGTGGTA CAGCTGCTGA GGTCATTCTT CTACATCACA GAGACCACGT 
     2701 TCCAGAAGAA CCGCCTTTTC TTCTACCGTA AGAGTGTGTG GAGCAAGCTG CAGAGCATTG 
     2761 GAATCAGGCA ACAGCTTGAG AGAGTTCAGC TACGGGAACT GTCACAAGAG GAGGTCAAGC 
     2821 ATCACCAGGA CACTTGGCTG GCCATGCCTA TCTGCAGATT GCGCTTCATC CCCAAGCTCA 
     2881 ATGGTCTCCG GCCCATTGTG AACATGAGTT ATGGCATGGA CACCAGAGCT TTTGGCAAAA 
     2941 AGAAGCAGAC CCAGTGTTTC ACTCAGAGTC TCAAGACTTT GTTCAGCGTG CTCAACTACG 
     3001 AGCGGACCAA ACATCCTAAC CTTATGGGTG CTTCAGTACT GGGTACGAGT GACAGCTACA 
     3061 GGATCTGGCG GACCTTCGTG CTGCGTGTGC GTGCTCTGGA CCAGACACCC AGGATGTACT 
     3121 TTGTTAAGGC AGATGTGACA GGGGCCTATG ATGCCATCCC CCAGGACAAG CTCGTGGAAA 
     3181 TTGTCGCCAA TATAATCAGG CGCTCAGAGA GCATGTACTG TATCCGCCAG TATGCAGTGG 
     3241 TTCAGAAAGA TAGCCAAGGC CAAGTCCACA AGTCCTTCAG GAGACAGGTC TCCACCCTCT 
     3301 CTGACCTCCA GCCATACATG GGCCAGTTCA CCAAGCATCT GCAGGACTCA GATGCCAGTG 
     3361 CACTGAGGAA CTCTGTTGTC ATCGAGCAGA GCATCTCCAT GAATGAGACT GGCAGTAGCC 
     3421 TGCTCCACTT CTTCCTGCGC TTTGTCCGTC ACAGTGTCGT GAAGATCGAT GGCAGGTTCT 
     3481 ATGTGCAATG CCAGGGCATC CCCCAGGGCT CCAGCCTGTC CACCCTGCTC TGCAGTCTGT 
     3541 GTTTCGGAGA CATGGAGAAC AAGCTGTTTG CCGAGGTGCA GCAGGACGGC TTGCTTTTAC 
     3601 GTTTTGTCGA TGACTTTCTG TTGGTGACAC CTCACCTGGC CCATGCAAAA GCCTTTCTCA 
     3661 GCACCCTGGT CCATGGCGTG CCCGAGTATG GCTGCATGAT AAACTTGCAG AAGACAGTGG 
     3721 TGAACTTCCC TGTGGAGACC GGCGCCCTGG GAGGTGCAGC CCCGCACCAG CTGCCTGCTC 
     3781 ACTGCCTGTT TCCCTGGTGT GGCTTACTGC TGGACACTCG GACTTTGGAA GTATTCTGTG 
     3841 ACTACTCAGG TTACGGACGG ACCTCAATTA AGATGAGCCT CACCTTCCAG GGTGTCTCCA 
     3901 GGGCCGGGAA GACCATGCGG TACAAGCTCT TGTCAGTCTT GCGGTTGAAG TGTCATGGTC 
     3961 TGTTTCTAGA CTTGCAGGTG AACAGCCTGC AGACAGTCTG CATCAATATA TACAAGATCT 
     4021 TCCTGCTTCA GGCCTACAGG TTCCATGCAT GTGTGATTCG GCTTCCCTTT GGCCAGCATG 
     4081 TTAGGAAGAA CCATGCATTC TTTCTGGGCA TCATCTCCAA CCTAGCATCC TGCTGCTACG 
     4141 CCATCCTGAA GGTCAAGAAT CCAGGAGTGT CACTAAGGGC CAAGGGTGCC CCTGGCTCCT 
     4201 TTCCGCCCGA GGCCACACGT TGGCTCTGCT ACCAAGCCTT CCTGCTCAAG CTGGCTGCTC 
     4261 ATTCTGTCAC CTACAAGTGT CTCCTGGGAC CTCTTAGGAC AGCCCAAAAA CAGCTGTGCC 
     4321 GGAAGCTCCC AGAGGCAACA ATGACCCTCC TTAAGACTGC AGCTGACCCA GCCCTAAGCA 
     4381 CAGATTTTCA GACCATTTTG GACACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4441 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4501 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4561 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4621 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4681 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4741 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4801 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4861 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4921 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4981 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5041 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5101 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5161 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5221 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5281 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5341 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5401 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5461 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5521 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5581 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5641 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5701 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5761 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5821 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5881 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5941 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6001 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6061 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6121 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6181 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6241 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6301 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6361 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6421 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6481 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6541 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6601 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6661 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6721 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6781 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6841 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6901 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6961 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7021 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7081 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7141 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7201 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7261 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7321 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7381 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7441 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7501 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7561 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7621 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7681 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7741 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7801 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7861 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7921 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7981 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8041 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8101 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8161 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8221 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//