LOCUS       RR210628                9462 bp    DNA     circular  SYN 
DEFINITION  Arhgef11 (Myc-DDK-tagged ORF) - Rat Rho guanine nucleotide exchange factor (GEF) 11 (Arhgef11), (10 ug)
ACCESSION   NM_023982
VERSION     NM_023982.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5619..5710 
                     /label="Myc-DDK"  
     misc_feature    5740..6325 
                     /label="PolyA signal"  
     misc_feature    5797..5816 
                     /label="Restriction Enzyme"  
     misc_feature    6601..7273 
                     /label="ColE1"  
     misc_feature    7703..8497 
                     /label="Kan/Neo"  
     misc_feature    8532..8834 
                     /label="SV40 ori"  
     misc_feature    8920..9397 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5601..5607 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5601 
                     /label="Arhgef11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCATACGA TTGCCCCATA GTATAGACAG ATCAGCCAGT AAAAAGCAGT 
     1081 CTCACCTGTC CAGTCCCATT GCATCCTGGT TAAGTAGCCT GTCTTCTCTG GGAGATTCTA 
     1141 CACCTGAACG CACATCCCCT TCTCACCACC GCCAGCCCTC TGACACTTCT GAGACAACAG 
     1201 CAGGTCTTGT TCAGCGCTGT GTCATCATCC AAAAGGACCA GCATGGCTTT GGCTTCACAG 
     1261 TTAGTGGAGA TCGCATTGTA CTGGTGCAGT CCGTGCGCCC TGGAGGCGCA GCCATGAAAG 
     1321 CTGGTGTGAA AGAGGGTGAC CGGATCATCA AAGTAAACGG CACCATGGTG ACCAATAGCT 
     1381 CACACCTGGA GGTGGTAAAG CTTATCAAAT CTGGCGCCTA TGCTGCGCTT ACCCTCCTAG 
     1441 GCTCTTCTCC TCCCTCCGTC GGCGTCTCTG GGCTCCAGCA GAATCCATCT GTGGCAGGAG 
     1501 TGCTCAGAGT TAACCCCATC ATTCCTCCAC CACCTCCCCC GCCACCCTTG CCACCTCCAC 
     1561 AGCACATTAC TGGACCCAAA CCTCTTCAGG ATCCTGAAGT CCAAAAGCAC GCCACTCAAA 
     1621 TCCTCTGGAA TATGCTAAGA CAGGAGGAGG AAGAGTTACA GGACATACTT CCACCCTGTG 
     1681 GTGAGACCAG TCAGAGAACA TGTGAGGGCC GCCTCTCTGT GGACTCCCAG GAGGCAGACA 
     1741 GTGGCTTGGA TTCTGGGACA GAACGCTTTC CCTCCATCAG TGAGTCATTG ATGAATCGGA 
     1801 ACTCAGTATT GTCAGATCCT GGACTAGACA GCCCTCAAAC CTCCCCTGTA ATCCTGGCCA 
     1861 GGGTGGCCCA GCACCACAGG CGACAGGGCT CAGATGCAGC GTTGCTCCCG CTCAACCACC 
     1921 AGGGTATAGA TCAAAGCCCA AAGCCTCTGA TTATTGGCCC AGAGGAAGAT TATGACCCAG 
     1981 GTTATTTCAA CAATGAGAGT GACATCATCT TCCAAGATCT TGAAAAACTG AAGTCACATC 
     2041 CAGCTTACTT GGTAGTTTTT CTACGTTACA TCCTCTCTCA GGCAGACCCT GGCCCCCTGC 
     2101 TTTTTTATTT GTGTTCAGAA GTTTATCAAC AGACAAATCC CAAAGATTCC CGAAGTCTGG 
     2161 GGAAAGACAT CTGGAACATT TTCCTGGAGA AAAATGCGCC TCTCAGAGTG AAGATCCCTG 
     2221 AGATGTTGCA GGCTGAAATT GACCTACGCC TGCGGAACAA TGAGGACCCT CGCAATGTGC 
     2281 TCTGTGAAGC TCAGGAGGCA GTCATGCTGG AAATCCAGGA GCAGATCAAC GACTACAGAT 
     2341 CCAAGCGTAC TCTGGGCCTG GGCAGCCTCT ATGGTGAAAA TGACCTGCTA GGCCTGGATG 
     2401 GGGACCCTCT TCGAGAACGC CAAATGGCTG AGAAGCAGCT GGCTGCCCTT GGAGATATCT 
     2461 TGTCCAAATA TGAGGAAGAT CGGAGTGCCC CCATGGACTT TGCTGTTAAT ACCTTTATGA 
     2521 GCCACGCTGG GATCCGTCTT CGGGAGTCTC GATCCTCCTG CACGGCAGAA AAGACCCAGT 
     2581 CTGCCCCTGA CAAGGACAAG TGGCTGCCCT TCTTCCCTAA GACCAAGAAG CAGAGCAGCA 
     2641 ATTCCAAGAA AGAAAAGGAT GCCTTGGAGG ACAAGAAGCG AAACCCCATC CTCAGATATA 
     2701 TTGGGAAGCC CAAGAGCTCC TCTCAGAGCA TTAAGCCAGG CAATGTGAGG AACATCATTC 
     2761 AGCACTTTGA GAACAGCCAT CAGTATGATG TCCCAGAGCC GGGGACACAA CGACTCTCAA 
     2821 CAGGAAGCTT TCCTGAGGAC CTGCTGGAGA GTGACAGTTC GCGCTCAGAG ATTCGACTGG 
     2881 GCCGCTCTGG GAGCCTCAAG GGCCGGGAAG AGATGAAGCG ATCCCGGAAA GCAGAGAACG 
     2941 TGCCCCGGCC TCGAAGTGAC GTTGACATGG ATGCTGCTGC AGAGGCTGCC CGCCTTCACC 
     3001 AGTCAGCCTC GTCCTCTGCC TCCAGCCTCT CCACCAGGTC TCTTGAGAAC CCAACCCCTC 
     3061 CCTTCACCCC CAAAATGGGC CGCAGGAGCA TTGAGTCCCC CAATCTGGGG TTCTGTACAG 
     3121 ACGTCATCCT TCCCCACCTC CTGGAGGATG ATCTGGGCCA ATTGTCTGAC CTGGAGCCAG 
     3181 AGCCAGAGGT CCAAAACTGG CAGCATACAG TAGGCAAGGA TGTGGTGGCC AACCTGACCC 
     3241 AGAGGGAAAT TGACCGGCAA GAGGTCATCA ATGAGCTTTT TGTGACAGAA GCATCTCACC 
     3301 TGCGCACACT CCGAGTCCTG GACCTCATCT TCTACCAGCG CATGAGAAAG GAGAACCTAA 
     3361 TGCCTCGGGA AGAGCTAGCG CGGCTCTTCC CTAACCTGCC TGAGCTCATA GAGATTCACA 
     3421 ATTCCTGGTG TGAGGCCATG AAGAAGCTCC GGGAGGAGGG CCCCATTATC AGAGACATCA 
     3481 GTGACCCCAT GCTGGCTCGG TTTGATGGTC CTGCCCGAGA AGAACTCCAG CAAGTAGCTG 
     3541 CACAATTCTG TTCCTATCAG TCAGTAGCCC TAGAGCTAAT CAGGACTAAG CAACGTAAGG 
     3601 AGAGTCGGTT CCAGCTCTTC ATGCAGGAGG CTGAGAGCCA CCCTCAGTGC CGGCGTCTGC 
     3661 AGCTCCGAGA CCTCATCGTC TCTGAAATGC AACGGCTCAC CAAGTACCCA CTGCTGCTAG 
     3721 AGAACATCAT CAAGCACACA GAGGGTGGCA CCTCTGAGCA TGAGAAGCTC TGCCGTGCCC 
     3781 GGGACCAGTG CCGGGAGATT CTCAAGTTTG TGAATGAAGC AGTAAAGCAG ACAGAGAACC 
     3841 GCCACCGGCT AGAGGGGTAC CAGAAACGCC TGGATGCCAC TGCCCTAGAG CGGGCCAGCA 
     3901 ACCCCTTGGC AGCAGAGTTC AAGAGCCTGG ATCTTACAAC AAGGAAGATG ATCCACGAGG 
     3961 GGCCTCTGAC CTGGAGGATC AGCAAAGACA AGACCCTGGA CCTCCAGGTG CTTCTGCTTG 
     4021 AGGACCTGGT GGTACTGCTG CAGAGACAAG AGGAGCGGCT GCTGCTAAAG TGCCACAGCA 
     4081 AGACAGCCGT GGGCTCCTCC GACAGCAAGC AGACGTTCAG CCCTGTGCTG AAGCTCAATG 
     4141 CTGTGCTCAT CCGCTCCGTG GCTACAGACA AGCGAGCCTT CTTCATCATC TGCACCTCCG 
     4201 AGCTGGGCCC TCCCCAGATC TATGAGCTGG TTGCATTGAC GTCATCAGAC AAGAATATAT 
     4261 GGATGGAGCT CTTAGAAGAG GCCGTGCAGA ATGCCACCAA GCACCCTGGA GCTGCCCCAA 
     4321 TCCCCATCCA TCCCTCACCA CCAGGATCCC AGGAGCCGGC CTACCAGGGC TCCACCTCCA 
     4381 GCAGGGTAGA AATAAATGAC TCAGAAGTAT ATCACACTGA AAAAGAACCC AAGAAGCTAC 
     4441 CTGAAGGCCC CGGGCCTGAG CAGAGAGTTC AAGACAAGCA GCTGATAGCA CAAGGGGAGC 
     4501 CTGTGCAGGA AGAGGATGAA GAGGAATTGA GGACCTTGCC TCGAGCTCCC CCCTCCCTGG 
     4561 ATGGAGAAAA CAGAGGCATC AGGACAAGGG ACCCTGTCCT TCTGGCCCTC ACAGGCCCTC 
     4621 TGCTCATGGA GGGACTTGCT GATGCTGCCC TGGAAGATGT GGAGAACTTG CGTCACCTGA 
     4681 TCCTGTGGAG CCTGCTGCCT GGTCACACTG TGAAGACTCA GGCTGCTGGC GAGCCTGAGG 
     4741 ATGACCTCAC ACCCACCCCT TCTGTCGTGA GCATCACCTC TCACCCCTGG GACCCAGGGT 
     4801 CCCCAGGGCA AGCTCCCACC ATAAGTGACA GCACCCGACT TGCGAGGCCA GAGGGCAGCC 
     4861 AGCCAGAGGG CGAGGATGTT GCTGTCAGTT CTCTGGCACA CCTGCCGCCA AGGACCAGAA 
     4921 GTTCTGGCGT CTGGGACTCT CCTGAGCTGG ATAGGAATCC GGCTGCAGAG GCTGCAAGCA 
     4981 CAGAACCAGC AGCAAGTTAC AAGGTTGTGA GAAAAGTCTC TCTACTCCCT GGTGGTGGTG 
     5041 TCGGTGCAGC CAAGGTGGCG GGCAGCAATG CTATCCCTGA CAGTGGCCAG TCAGAATCTG 
     5101 AGCTATCTGA AGTGGAAGGC GGAGCACAGG CTACGGGGAA CTGTTTCTAT GTCAGCATGC 
     5161 CAGCAGGACC TCTGGACTCC AGCACTGAGC CTACTGGGAC ACCCCCAAGC CCCTCACAGT 
     5221 GTCACAGCCT CCCTGCATGG CCAACAGAGC CTCAGCCCTA CAGGGGAGTC CGTGGGGGTC 
     5281 AGTGTTCCAG CCTGGTCCGC AGGGATGTGG ATGTGATCTT CCATACCATC GAGCAGCTCA 
     5341 CCATCAAGCT TCACAGACTG AAGGACATGG AGCTGGCCCA CAGAGAGCTG CTCAAGTCCC 
     5401 TTGGAGGAGA GTCATCGGGT GGAACCACAC CTGTGGGGAG TTTTCACACA GAGGCAGCCA 
     5461 GATGGACAGA CTACTCCCTC TCTCCTCCAG CCAAGGAAGC CCTGGCCTCT GATTCCCAAA 
     5521 ATGGTCAGGA GCAGGGGTCC TGCCCTGAAG AAGGCTCCGA CATCGCCCTG GAAGACAGTG 
     5581 CCACTGACAC AGCTGTGTCA CCAGGACCAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5641 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5701 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5761 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5821 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5881 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5941 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     6001 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     6061 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     6121 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     6181 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     6241 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     6301 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6361 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6421 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6481 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6541 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6601 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6661 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6721 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6781 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6841 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6901 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6961 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     7021 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     7081 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     7141 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     7201 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     7261 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     7321 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7381 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7441 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7501 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7561 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7621 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7681 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7741 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7801 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7861 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7921 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7981 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     8041 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     8101 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     8161 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     8221 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     8281 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     8341 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8401 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8461 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8521 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8581 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8641 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8701 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8761 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8821 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8881 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8941 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     9001 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     9061 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     9121 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     9181 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     9241 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     9301 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9361 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9421 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//