LOCUS       RR210622                8538 bp    DNA     circular  SYN 
DEFINITION  Plcb1 (Myc-DDK-tagged ORF) - Rat phospholipase C, beta 1 (phosphoinositide-specific) (Plcb1), (10 ug)
ACCESSION   NM_001077641
VERSION     NM_001077641.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4693..4784 
                     /label="Myc-DDK"  
     misc_feature    4814..5399 
                     /label="PolyA signal"  
     misc_feature    4871..4890 
                     /label="Restriction Enzyme"  
     misc_feature    5675..6347 
                     /label="ColE1"  
     misc_feature    6777..7571 
                     /label="Kan/Neo"  
     misc_feature    7606..7908 
                     /label="SV40 ori"  
     misc_feature    7994..8471 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4668..4675 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4668 
                     /label="Plcb1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGGGGCT CAGCCCGGAG TGCACGCCTT GCAACTCAAG CCCGTGTGCG 
     1081 TGTCCGACAG CCTCAAGAAG GGCACCAAAT TCGTCAAGTG GGATGATGAC TCCACTATAG 
     1141 TTACTCCAAT TATTTTGAGG ACCGATCCTC AGGGATTTTT CTTTTACTGG ACAGATCAGA 
     1201 ATAAGGAGAC GGAGCTGTTA GATCTCAGCC TCGTCAAGGA TGCCAGGTGT GGGAAGCACG 
     1261 CCAAAGCTCC CAAGGACCCC AAGTTACGTG AACTTCTGGA TGTTGGGAAC ATCGGACACT 
     1321 TGGAACAGCG CATGATAACT GTGGTGTATG GGCCAGACCT GGTGAATATC TCCCACCTGA 
     1381 ATCTTGTGGC TTTTCAAGAG GAAGTGGCCA AGGAATGGAC AAATGAGGTT TTCAGTTTGG 
     1441 CAACAAACCT GCTGGCTCAG AACATGTCCA GGGACGCATT TCTGGAGAAA GCATATACTA 
     1501 AGCTCAAGCT TCAGGTGACC CCAGAAGGGC GCATTCCTCT TAAAAACATC TATCGACTGT 
     1561 TTTCGGCAGA CCGGAAGCGA GTGGAAACTG CGCTAGAGGC TTGTAGTCTT CCATCGTCAA 
     1621 GGAACGATTC CATCCCTCAA GAGGACTTTA CTCCAGATGT ATACAGAGTT TTCCTGAACA 
     1681 ATCTTTGTCC CCGACCTGAA ATTGATAACA TCTTCTCTGA ATTTGGTGCC AAAAGCAAAC 
     1741 CGTACCTTAC TGTTGATCAG ATGATGGATT TTATCAACCT TAAGCAGAGA GATCCCCGGC 
     1801 TGAATGAAAT ACTTTACCCA CCTCTGAAGC AAGAGCAGGT CCAAGTGTTG ATTGAGAAGT 
     1861 ACGAGCCCAA CAGCAGCCTC GCCAAGAAAG GGCAGATGTC AGTGGATGGA TTCATGCGCT 
     1921 ACCTGAGCGG AGAAGAAAAT GGAGTCGTTT CACCTGAGAA ACTGGATTTG AACGAAGACA 
     1981 TGTCTCAGCC CCTGTCTCAC TATTTCATCA ATTCCTCACA CAACACCTAC CTCACAGCTG 
     2041 GCCAGTTGGC TGGGAACTCG TCTGTAGAGA TGTATCGCCA GGTGCTTCTG TCTGGATGTC 
     2101 GCTGTGTGGA GCTGGACTGC TGGAAGGGCA GGACCGCTGA GGAAGAGCCT GTCATCACCC 
     2161 ATGGATTCAC CATGACAACA GAAATATCCT TCAAGGAAGT CATAGAAGCC ATCGCAGAGT 
     2221 GTGCGTTCAA GACTTCTCCT TTTCCCATCC TCCTTTCCTT TGAGAACCAT GTGGATTCCC 
     2281 CGAAGCAACA AGCCAAGATG GCCGAGTATT GCCGATTAAT CTTTGGTGAT GCCCTCCTTA 
     2341 TGGAGCCACT GGAAAAATAC CCACTGGAAT CTGGGGTACC TCTTCCAAGC CCTATGGATT 
     2401 TAATGTATAA AATCTTGGTG AAAAACAAGA AGAAGTCACA CAAGTCGTCA GAGGGAAGTG 
     2461 GTAAAAAGAA GCTCTCTGAG CAAGCTTCCA ACACGTACAG CGACTCTTCC AGCGTGTTCG 
     2521 AGCCTTCGTC TCCGGGAGCT GGGGAAGCAG ATACGGAGAG TGATGACGAT GACGACGATG 
     2581 ATGACTGTAA AAAGTCTTCC ATGGATGAGG GGACTGCTGG CAGCGAGGCC ATGGCCACAG 
     2641 AAGAGATGTC TAACCTGGTG AACTATATTC AGCCTGTCAA GTTTGAGTCC TTTGAAACTT 
     2701 CAAAAAAAAG AAATAAAAGC TTTGAAATGT CTTCCTTCGT GGAAACCAAA GGACTCGAAC 
     2761 AACTCACGAA GTCTCCAGTT GAATTTGTCG AATACAACAA GATGCAGCTT AGCAGGATAT 
     2821 ATCCCAAAGG AACACGCGTG GACTCATCCA ACTACATGCC TCAGCTCTTC TGGAATGCTG 
     2881 GCTGTCAGAT GGTGGCGCTC AACTTCCAGA CAGTGGATCT AGCTATGCAG ATAAACATGG 
     2941 GCATGTACGA ATACAATGGG AAGAGTGGCT ACAGGCTGAA GCCAGAGTTC ATGAGGAGGC 
     3001 CAGACAAGCA TTTTGATCCA TTTACTGAAG GAATCGTAGA TGGGATAGTG GCCAACACTT 
     3061 TATCTGTTAA GATTATTTCA GGTCAGTTTC TCTCTGATAA GAAAGTTGGG ACTTATGTGG 
     3121 AAGTGGATAT GTTTGGTTTG CCTGTGGACA CAAGAAGGAA GGCATTTAAA ACCAAGACAT 
     3181 CCCAAGGAAA TGCTGTAAAT CCTGTCTGGG AAGAAGAGCC AATTGTATTC AAAAAGGTAG 
     3241 TTCTGCCTTC TCTGGCCTGT TTAAGGATAG CAGCATATGA GGAAGGAGGC AAATTTATTG 
     3301 GCCACCGGAT CTTGCCTGTG CAAGCAATTC GGCCAGGCTA TCACTACATC TGCCTGCGGA 
     3361 ATGAGAGGAA CCAGCCCCTG ATGCTGCCAG CTGTCTTTGT CTACATAGAA GTCAAAGACT 
     3421 ATGTCCCAGA CACGTATGCA GATGTAATTG AAGCATTATC AAACCCAATC CGATATGTCA 
     3481 ATCTGATGGA ACAGAGAGCT AAGCAGTTGG CTGCATTGAC ACTGGAGGAT GAAGAGGAAG 
     3541 TCAAGAAGGA GGCTGACCCC GGGGAAACGT CATCCGAGGC TCCAAGTGAA ACCAGGACAA 
     3601 CTCCAGCAGA GAATGGGGTG AATCACACCG CAACCCTTGC ACCCAAGCCA CCTTCCCAGG 
     3661 CTCCACACAG CCAGCCTGCT CCAGGGTCTG TGAAGGCACC CGCCAAAACA GAGGATCTGA 
     3721 TTCAGAGCGT GTTAACAGAA GTAGAGGCGC AGACCATTGA AGAGCTCAAG CAACAGAAAT 
     3781 CGTTCGTGAA ACTTCAAAAG AAGCACTACA AAGAAATGAA AGACCTGGTC AAGAGACACC 
     3841 ACAAGAAAAC CACCGAGCTC ATCAAGGAGC ATACCACCAA GTACAATGAG ATTCAGAATG 
     3901 ACTACTTGAG AAGGAGGGCA GCCTTGGAAA AGTCCGCCAA AAAGGATAGC AAGAAGAAAT 
     3961 CTGAACCCAG CAGCCCAGAT CATGGCTCAT CCGCCATTGA GCAAGACCTC GCGGCCCTGG 
     4021 ATGCAGAAAT GACTCAGAAG TTGATAGACT TGAAAGACAA GCAACAACAG CAGCTGCTTA 
     4081 ATCTTCGGCA AGAGCAGTAT TACAGTGAGA AGTACCAGAA GCGGGAGCAC ATTAAATTGC 
     4141 TCATTCAGAA GTTGACAGAT GTTGCTGAAG AGTGTCAGAA CAATCAGTTG AAGAAGCTGA 
     4201 AGGAAATCTG CGAGAAAGAG AAGAAGGAAT TAAAGAAGAA AATGGATAAG AAGAGGCAGG 
     4261 AGAAGATAAC AGAAGCCAAG TCCAAAGACA AAAGCCAGAT GGAAGAGGAG AAGACAGAGA 
     4321 TGATCCGATC ATACATCCAG GAGGTGGTGC AGTACATCAA GAGGTTAGAG GAAGCACAAA 
     4381 GTAAAAGACA AGAAAAACTT GTGGAAAAAC ACAAGGAGAT CCGCCAGCAG ATCCTGGATG 
     4441 AGAAGCCCAA GCTGCAGATG GAGCTGGAGC AAGAATACCA AGACAAGTTC AAAAGACTGC 
     4501 CCCTGGAGAT TCTGGAGTTT GTACAGGAAG CCATGAAAGG GAAGGTTAGT GAGGACAGCA 
     4561 ATCACGGCTC TGCCCCTCCC TCGCTGGCCT CAGACCCTGC TAAGGTGAAC CTCAAGTCTC 
     4621 CCTCCAGTGA GGAGGTACAA GGAGAGAACG CGGGAAGAGA GTTTGATACT CCTCTGAGCG 
     4681 GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4741 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4801 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4861 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4921 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4981 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5041 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5101 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5161 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5221 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5281 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5341 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5401 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5461 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5521 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5581 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5641 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5701 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5761 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5821 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5881 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5941 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6001 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6061 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6121 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6181 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6241 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6301 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6361 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6421 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6481 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6541 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6601 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6661 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6721 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6781 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6841 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6901 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6961 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7021 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7081 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7141 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7201 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7261 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7321 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7381 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7441 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7501 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7561 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7621 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7681 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7741 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7981 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8041 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8101 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8161 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8221 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8281 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8341 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8401 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8461 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8521 AAATTTAACG CGAATTTT
//