LOCUS       RR210576                7017 bp    DNA     circular  SYN 
DEFINITION  Ltf (Myc-DDK-tagged ORF) - Rat lactotransferrin (Ltf), (10 ug)
ACCESSION   NM_001106864
VERSION     NM_001106864.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3174..3265 
                     /label="Myc-DDK"  
     misc_feature    3295..3880 
                     /label="PolyA signal"  
     misc_feature    3352..3371 
                     /label="Restriction Enzyme"  
     misc_feature    4156..4828 
                     /label="ColE1"  
     misc_feature    5258..6052 
                     /label="Kan/Neo"  
     misc_feature    6087..6389 
                     /label="SV40 ori"  
     misc_feature    6475..6952 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    3156..3162 
                     /label="Restriction Enzyme"  
     misc_feature    1029..3156 
                     /label="Ltf"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG AGGCCGTTCA TCACTGTCTT GATGTTTCTT GGGGTCCTTG 
     1081 GACCCTTTCT AGCTAGGATA GATACAGTTG TTCGATGGTG CACCATATCA AGAAATGAGG 
     1141 CACAAAAATG TTTCATGTGG CAAGAGATGC TGAACAAAGC GGGTGTCCCG AAGCTTAGAT 
     1201 GTGCCAGGAA GTACTTCATG CCTCACTGTA TCCAGGAGAT TATGATGAGG AGAGCCGACG 
     1261 CCATGACTCT TTCTGGCAGC GCCATATTCG ATTTCTATTT TCCCTACAAA CTGCAGCCGA 
     1321 TAGCTGCCGA AGTCTATGGA ACCAAAGAGA AGCCCCGGAT TCACTATTAT GCGGTGGCCG 
     1381 TCGTGAAGAA CAGCAGTGAC ATTCGTCTGA ACCAACTCCA AGGCTTGAAG TCCTGCCATG 
     1441 CAGGCTTTGA CACTAGTGCA GGGTGGATAG CCCCTTTAGG GGCACTTCGT CCATACCTGA 
     1501 ATTGGGATGA GAAATCTGTA TCCCTTGAGG AAGCGGTATC CAAGTTCTTC TCACAGAGCT 
     1561 GTGTTCCTGG TATCAGCAAG TCTAGATTTC CCAGGTTGTG TAGCTTGTGT GCAGGGAAAG 
     1621 GAGAACACAT ATGTGACTTT TCCCCCCAGG AACCATACGC TGGCTACGCA GGTGCCTTCA 
     1681 GGTGTCTGAG AGACAACGCT GGAGATGTGG CTTTTATCAG AGAAAGCACG ATATTTGAGG 
     1741 AGTTACCCAA TGAAGCTGAA TGGGACCAGT ACAAGCTGCT TTGCCCAGAT AACACCTGGA 
     1801 AGCCGGTGAC AGAGTACAAG GAGTGCCACC TGGCTCAAAT CCCTTCACGT GCTGTCGTAG 
     1861 CCCATGTCAG AAGTGAAAAA GACAGTGCCA TCTGGGAGCT TCTCCACCTG TCACAGGAAA 
     1921 TGTTTGGAAA AAACAAAACA TCAAAATTCG AGCTCTTTGG CTCCTATCTG GGACAGAAGG 
     1981 ACCTGCTGTT CAAGGACTCT GTCATTGGCT TTGTGAGGGT TCCCTTTACA GTGAATGTTT 
     2041 GGCTTTACCT GACCTTCAGT TATATCATGG CCCTGAAGAG CTTGAAGGAA AGCAAGGAGA 
     2101 ATGTGATGGC CTTAAGGGCC CGGATCACGT GGTGTGCCGT GGGCAGTGAG GAGAAGCTCA 
     2161 AGTGTGACCA GTGGAGCAGA GTGAGTGTTG GCAAGATTAC CTGCATCTCA TGCACCACCA 
     2221 CGGAGCAGTG CATCTTCTCT ATCACGATGG GAGATGCTGA TGCCATGAAC TTGGATGGAG 
     2281 GCTATATCTA CTCTGCGGGC AAGTGTGGTT TAGTTCCAGT TTTGGCAGAG ATCCAGAAAT 
     2341 CCCCCAATAG CAATGGCTCT GATTGTGTGG ACAGACCAGC AGAAGGGTAC CTTGCTGTAG 
     2401 CCGCAGTCAG AAAAGAAGAT ACTGGCTTCA CCTGGAGCAC TGTGAGAGGG AAGAAATCCT 
     2461 GCCACACCGC TGTGGACAGG ACCGCGGGCT GGAACATTCC CATGGGCCTG CTTGTCAACC 
     2521 AGACCAACTC CTGCCAATTT AAGGAATTCT TTAACAAAAG CTGCGCCCCT GGGTCTTTCC 
     2581 TCTATTCCAA TCTCTGTGCC CTGTGTATTG GTGACGAGAA CGGTAAGGAT AAGTGTAACC 
     2641 CCAACAGCCA AGAGAGGTAC CAAGGCTACG TCGGGGCTTT CAGGTGTCTG GCTGAGAAGG 
     2701 CAGGAAATGT CGCGTTTTTG AAGGACGCCA CTGTCTTGCA GAATACAGAC GGGAAGAATG 
     2761 CTGACAAGTG GGCTAAGAAC TTGAAGCTGG ATGACTTCGA GCTGTTGTGC CTTGATGACA 
     2821 CCCGGAAGCC TGTGACTGAG GCTAAGAACT GCCACCTAGC CGTAGCCCCA AACCATGCTG 
     2881 TAGTAGCTCG GAAGGACAAG GCGAGACTCG TTCAGCAAGA GCTGCTTTAC CAACAGGTTC 
     2941 AGTTTGGGAG AAATGGATGC AGGTGTCCAG AAGAGTTTTG CCTGTTCAGG TCTGAAACCA 
     3001 AAAACCTTCT GTTCAATGAC AACACTGAGT GTCTGGCCAA GCTCCCCAGC AAAATCACAT 
     3061 GGGAGGAATA TCTAGGCAAG GAGTACGTCG TAGCGATCGC GCATCTGAGG CAGTGCTCCA 
     3121 ATATCACTCA GGAAGCCTAC AATTTTCTTG CCCTTCGAAA CTCCACGCGT ACGCGGCCGC 
     3181 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3241 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3301 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3361 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3421 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3481 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3541 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3601 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3661 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3721 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3781 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3841 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     3901 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     3961 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4021 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4081 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4141 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4201 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4261 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4321 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4381 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4441 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4501 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4561 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4621 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4681 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4741 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4801 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4861 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     4921 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     4981 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5041 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5101 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5161 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5221 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5281 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5341 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5401 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5461 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5521 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5581 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5641 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5701 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5761 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5821 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     5881 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     5941 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6001 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6061 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6121 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6181 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6241 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6301 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6361 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6421 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6481 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6541 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6601 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6661 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6721 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6781 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6841 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     6901 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     6961 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//