LOCUS       RR210573                8625 bp    DNA     circular  SYN 
DEFINITION  Adcy8 (Myc-DDK-tagged ORF) - Rat adenylate cyclase 8 (brain) (Adcy8), (10 ug)
ACCESSION   NM_017142
VERSION     NM_017142.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4782..4873 
                     /label="Myc-DDK"  
     misc_feature    4903..5488 
                     /label="PolyA signal"  
     misc_feature    4960..4979 
                     /label="Restriction Enzyme"  
     misc_feature    5764..6436 
                     /label="ColE1"  
     misc_feature    6866..7660 
                     /label="Kan/Neo"  
     misc_feature    7695..7997 
                     /label="SV40 ori"  
     misc_feature    8083..8560 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4764..4770 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4764 
                     /label="Adcy8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACTCTCG GATGTGCACT GCCTTAGCGG CAGCGAGGAA CTCTATACCA 
     1081 TTCACCCGAC GCCCCCGGCC GCGGACGGCG GGAGCGGCTC TCGGCCGCAG CGGCTGCTGT 
     1141 GGCAGACGGC GGTGCGGCAC ATCACCGAGC AGCGCTTCAT CCACGGCCAC CGAGGCGGCG 
     1201 GCGGCGGGGG CTCCCGCAAA GCCTCGAACC CTGCGGGCAG CGGACCAAAC CACCACGCGC 
     1261 CGCAGCTGTC TAGCGACTCG GTGCTGCCTC TCTATTCTCT GGGCTCCGGA GAGCGAGCGC 
     1321 ACAACACCGG TGGCACCAAA GTCTTTCCGG AACGCAGCGG GAGCGGCAGT GCCAGTGGCA 
     1381 GCGGGGGTGG GGGCGACTTG GGCTTCCTGC ACCTTGACTG TGCCCCAAGT AACTCGGATT 
     1441 TCTTCCTCAA TGGGGGATAC AGCTACCGTG GGGTCATTTT CCCAACCCTA CGCAACTCCT 
     1501 TCAAGTCTCG GGATCTGGAG CGCCTCTATC AACGCTATTT CCTGGGCCAG AGGCGCAAAT 
     1561 CGGAGGTAGT GATGAACGTG CTGGATGTAC TAACCAAACT CACCCTTCTA GTCCTTCACT 
     1621 TGAGCCTAGC CTCGGCTCCC ATGGACCCTC TCAAGGGCAT CCTGCTAGGC TTTTTCACTG 
     1681 GCATCGAGGT GGTGATCTGC GCCCTCGTGG TGGTCAGGAA GGACACCACC TCCCACACTT 
     1741 ACCTGCAATA CAGCGGCGTG GTCACTTGGG TGGCTATGAC CACCCAGATT CTGGCAGCAG 
     1801 GCCTGGGCTA TGGGCTTCTG GGCGACGGCA TAGGCTACGT GCTTTTTACA CTCTTCGCCA 
     1861 CCTACAGCAT GCTTCCGCTG CCTCTCACCT GGGCCATCTT GGCCGGCCTG GGCACATCCT 
     1921 TGCTGCAAGT CACACTTCAA GTGCTCATAC CCAGACTAGC GGTCTTTTCC ATCAACCAGG 
     1981 TCCTGGCCCA GGTGGTGCTC TTCATGTGCA TGAATACAGC AGGCATCTTC ATCAGTTACC 
     2041 TTTCAGACCG CGCCCAGCGG CAGGCCTTCC TGGAGACCCG GAGGTGTGTG GAGGCCAGGC 
     2101 TCCGCCTGGA GACAGAGAAC CAAAGACAGG AGCGGCTTGT GCTCTCTGTG CTCCCCAGGT 
     2161 TTGTCGTCCT AGAAATGATC AATGACATGA CCAATGTGGA GGACGAGCAC CTGCAGCATC 
     2221 AGTTCCACCG CATCTACATC CATCGCTACG AGAACGTCAG TATTCTTTTT GCAGATGTCA 
     2281 AAGGATTTAC CAACCTCTCT ACGACCTTGT CTGCTCAGGA GCTTGTCAGG ATGCTCAACG 
     2341 AGCTCTTTGC CAGATTTGAT CGGCTGGCCC ATGAGCATCA CTGTCTTCGC ATTAAAATCC 
     2401 TGGGGGACTG CTACTACTGT GTGTCAGGAC TGCCTGAGCC CCGCCAGGAC CATGCTCATT 
     2461 GCTGTGTTGA AATGGGCCTC AGCATGATCA AAACTATCAG GTTTGTGAGG TCCAGAACGA 
     2521 AGCATGATGT TGACATGCGA ATTGGAATCC ATTCAGGCTC TGTGCTGTGT GGTGTGTTGG 
     2581 GCCTGAGAAA ATGGCAGTTT GATGTCTGGT CTTGGGATGT GGACATCGCA AACAAACTTG 
     2641 AATCTGGAGG AATCCCTGGG AGAATTCACA TTTCCAAAGC CACACTGGAT TGCCTCAGTG 
     2701 GTGACTATAA TGTGGAAGAG GGCCACGGTA AGGAGAGGAA TGAATTCTTG AGGAAGCATA 
     2761 ATATAGAGAC CTATTTGATT AAGCAGCCCG AGGAGAGTTT GCTATCCTTG CCTGAAGATA 
     2821 TAGTTAAGGA GTCGGTGAGC TGCTCGGACA GGAGAAACAG TGGGGCAACG TTCACAGAAG 
     2881 GATCCTGGAG CCCAGAACTG CCATTCGACA ACATCGTGGG CAAACAGAAT ACTCTGGCTG 
     2941 CCCTAACAAG AAATTCAATA AATCTGCTTC CAAACCATCT CGCACAAGCT TTGCATGTCC 
     3001 AGTCTGGGCC TGAGGAAATT AACAAGAGAA TAGAGCATAC CATCGACTTG CGGAGTGGCG 
     3061 ATAAGTTGAG AAGAGAGCAT ATCAAGCCAT TCTCACTGAT GTTTAAAGAC TCCAGCCTGG 
     3121 AGCACAAGTA TTCTCAAATG CGGGATGAAG TATTCAAGTC AAACTTGGTC TGTGCATTTA 
     3181 TCGTTCTTCT GTTTATCACT GCGATTCAAA GTTTGCTTCC TTCTTCGAGG CTGATGCCTA 
     3241 TGACCATCCA GTTCTCCATC CTGATCATGC TGCACTCCGC CCTGGTCCTC ATCACCACGG 
     3301 CAGAAGACTA TAAGTGTCTG CCTCTCATTC TCCGCAAAAC CTGTTGTTGG ATTAACGAGA 
     3361 CCTATTTGGC CCGCAACGTC ATCATCTTTG CTTCCATCTT GATTAATTTC CTGGGAGCCG 
     3421 TCATAAATAT TCTGTGGTGT GATTTTGACA AGTCGATACC CTTGAAGAAC CTGACTTTCA 
     3481 ATTCCTCAGC TGTGTTTACA GATATCTGCT CCTACCCAGA GTACTTTGTC TTCACTGGGG 
     3541 TGTTGGCCAT GGTGACGTGT GCAGTATTTC TCCGGCTTAA CTCTGTCCTG AAGCTGGCTG 
     3601 TGCTGCTCAT TATGATCGCC ATCTACGCCC TGCTGACAGA GACCATCTAT GCAGGTCTCT 
     3661 TTCTGAGTTA TGACAACCTG AACCACAGTG GAGAAGATTT TCTGGGGACC AAGGAGGCAT 
     3721 CACTGCTACT GATGGCCATG TTCCTTCTTG CTGTATTCTA CCATGGACAG CAGCTGGAGT 
     3781 ACACAGCCCG CCTAGATTTC CTGTGGCGAG TACAGGCCAA AGAGGAGATC AACGAGATGA 
     3841 AGGACTTGAG GGAACACAAT GAGAACATGC TTCGCAATAT CTTACCCGGC CACGTGGCCC 
     3901 GCCACTTCCT GGAGAAAGAC AGAGACAATG AGGAGCTGTA TTCTCAATCC TATGATGCCG 
     3961 TTGGGGTAAT GTTTGCCTCC ATTCCTGGAT TTGCAGACTT TTACTCTCAG ACAGAAATGA 
     4021 ACAACCAGGG AGTGGAATGT CTGCGCTTGC TGAATGAGAT CATTGCTGAC TTTGATGAGT 
     4081 TACTTGGAGA GGACCGCTTT CAGGACATAG AGAAGATTAA GACCATTGGT AGTACATACA 
     4141 TGGCTGTCTC AGGACTGTCA CCAGAGAAAC AGCAATGTGA AGATAAATGG GGACATTTGT 
     4201 GTGCCCTGGC TGACTTCTCT CTTGCCCTGA CTGAAAGCAT ACAAGAGATC AACAAGCATT 
     4261 CGTTCAACAA TTTTGAACTC CGTATTGGCA TCAGCCATGG CTCAGTGGTA GCAGGTGTAA 
     4321 TTGGCGCTAA GAAACCACAG TATGACATTT GGGGTAAAAC TGTGAACTTG GCAAGCCGAA 
     4381 TGGACAGCAC AGGAGTGAGT GGCCGGATCC AAGTTCCTGA GGAGACCTAT CTCATCCTGA 
     4441 AGGATCAGGG CTTTGCCTTC GACTACCGGG GAGAGATATA TGTGAAGGGC ATCAGCGAAC 
     4501 AAGAAGGGAA AATCAAAACA TATTTTCTCC TGGGACGAGT CCAACCCAAC CCATTCATCT 
     4561 TACCCCCAAG AAGACTTCCT GGGCAGTACT CTCTGGCTGC AGTTGTCCTT GGGCTTGTCC 
     4621 AGTCTCTCAA CAGGCAAAGG CAGAAGCAAC TACTCAATGA GAACAGCAAC TCTGGCATCA 
     4681 TCAAGAGCCA TTACAACCGG CGGACTTTGC TAACGCCAAG TGGGCCAGAG CCTGGAGCAC 
     4741 AGGCTGAAGG CACTGACAAA TCCGATTTGC CAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4801 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4861 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4921 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4981 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5041 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5101 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5161 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5221 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5281 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5341 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5401 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5461 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5521 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5581 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5641 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5701 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5761 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5821 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5881 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5941 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6001 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6061 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6121 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6181 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6241 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6301 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6361 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6421 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6481 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6541 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6601 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6661 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6721 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6781 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6841 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6901 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6961 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7021 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7081 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7141 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7201 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7261 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7321 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7381 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7441 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7501 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7561 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7621 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7681 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7741 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7801 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7861 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7921 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7981 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8041 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8101 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8161 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8221 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8281 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8341 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8401 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8461 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8521 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8581 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//