LOCUS       RR210558                8211 bp    DNA     circular  SYN 
DEFINITION  Gucy2d (Myc-DDK-tagged ORF) - Rat guanylate cyclase 2d (Gucy2d), (10 ug)
ACCESSION   NM_130737
VERSION     NM_130737.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4368..4459 
                     /label="Myc-DDK"  
     misc_feature    4489..5074 
                     /label="PolyA signal"  
     misc_feature    4546..4565 
                     /label="Restriction Enzyme"  
     misc_feature    5350..6022 
                     /label="ColE1"  
     misc_feature    6452..7246 
                     /label="Kan/Neo"  
     misc_feature    7281..7583 
                     /label="SV40 ori"  
     misc_feature    7669..8146 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4350..4356 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4350 
                     /label="Gucy2d"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGGTCTG CAGCAGGGCT GTCACCCCGA AGGCCAGGAC TGGACAGCGC 
     1081 CACACTGGAA GACCTGCCGG GCCCTGCCAG GGCCACGGGG ACTCACGGTC AGACATCTGA 
     1141 GGACAGTCTC TTCCATATCG GTCTTCTCTG TGGTATTTTG GGGTGTCTTG CTGTGGGCTG 
     1201 ACTCTCTTTC CCTGCCAGCA TGGGCAAGAG AGACCTTCAC CCTTGGGGTG CTGGGTCCCT 
     1261 GGGACTGTGA CCCTATCTTT GCCCAGGCAC TCCCTAGCAT GGCTACCCAG TTGGCTGTAG 
     1321 ATCGAGTCAA TCAGGACGCC TCACTGCTGC TGGGCTCACA GTTGGATTTC AAGATCCTCC 
     1381 CTACAGGCTG TGACACCCCT CATGCCCTGG CCACATTTGT GGCCCACAGG AACACAGTGG 
     1441 CTGCTTTTAT AGGCCCTGTC AATCCTGGGT ATTGTCCAGC AGCAGCTCTG CTGGCCCAAG 
     1501 GCTGGGGCAA GTCCCTCTTC TCCTGGGCCT GTGGAGCTCC AGAGGGAGGA GGTGCCTTAG 
     1561 TGCCCACTTT GCCTTCTATG GCCGACGTGC TACTGTCTGT CATGAGACAC TTTGGCTGGG 
     1621 CTCGCTTGGC CATCGTGTCC TCTCACCAGG ACATCTGGGT AACCACAGCC CAGCAGCTAG 
     1681 CCACAGCTTT CAGGGCCCAT GGGCTGCCTA TCGGACTGAT CACCTCCTTG GGACCTGGAG 
     1741 AGAAGGGGGC CACGGAGGTT TGCAAGCAGC TCCACAGTGT GCATGGTCTG AAAATCGTGG 
     1801 TTCTGTGCAT GCACTCAGCG CTGCTTGGAG GCCTAGAGCA GACAGTCCTG CTGCGCTGCG 
     1861 CGAGGGAGGA GGGCCTGACA GATGGCAGAC TGGTCTTCTT GCCCTACGAC ACGCTGCTCT 
     1921 TTGCCCTGCC CTATCGTAAC CGCTCTTACC TGGTCCTGGA TGATGATGGG CCCCTTCAGG 
     1981 AGGCCTACGA TGCAGTACTC ACCATCAGTC TGGACACCAG TCCTGAGAGC CATGCCTTCA 
     2041 CTGCTACCAA GATGAGAGGA GGGGCAGCTG CCAACTTGGG GCCCGAGCAG GTGTCCCCAC 
     2101 TCTTTGGAAC TATCTACGAT GCCGTCATCC TGTTGGCCCA TGCCCTGAAC CACTCTGAGA 
     2161 CCCATGGAAC GGGGCTCTCA GGGGCTCACT TAGGGAACCA TATAAGAGCT CTTGATGTGG 
     2221 CTGGTTTTAG CCAGAGAATC CGGATAGATG GGAAAGGCAG AAGGCTACCC CAATACGTCA 
     2281 TCCTGGACAC AAATGGTGAA GGAAGCCAGT TGGTTCCCAC CCACATCCTG GACGTGAGCA 
     2341 CACAGCAGGT GCAGCCCCTG GGAACGGCGG TACACTTCCC TGGAGGGAGC CCTCCAGCCC 
     2401 ATGATGCCAG CTGCTGGTTC GACCCCAACA CACTATGCAT ACGAGGTGTG CAGCCCCTGG 
     2461 GCAGCCTCCT GACTCTGACG ATAACCTGTG TCCTGGCTCT GGTTGGTGGA TTCCTTGCTT 
     2521 ACTTTATCAG GTTAGGCCTC CAGCAGCTGC GGCTGCTGCG GGGCCCCCAT CGGATCCTGC 
     2581 TGACACCTCA AGAGCTCACC TTCCTCCAGC GAACCCCCAG CCGGCGGAGG CCACATGTGG 
     2641 ACAGTGGCAG CGAGTCAAGA AGTGTGGTAG ATGGTGGGAG TCCACAGTCA GTGATCCAGG 
     2701 GGTCAACAAG GAGCGTACCA GCCTTCCTGG AGCACACCAA CGTGGCCCTG TACCAGGGAG 
     2761 AGTGGGTGTG GCTGAAGAAG TTTGAGGCAG GCACGGCTCC TGATCTGCGG CCCAGCAGCC 
     2821 TCAGCCTCCT GAGAAAGATG CGGGAGATGC GGCATGAGAA TGTCACCGCC TTCCTGGGTC 
     2881 TCTTTGTGGG CCCTGAGGTT AGTGCGATGG TGCTGGAGCA CTGTGCCCGT GGCAGCCTGG 
     2941 AGGACCTGCT ACGGAATGAG GACCTGAGGC TAGACTGGAC CTTCAAGGCC TCCTTACTGT 
     3001 TGGATCTGAT CCGAGGTTTG CGGTATCTGC ACCATCGACA TTTCCCCCAT GGGCGCCTCA 
     3061 AGTCCAGGAA CTGTGTGGTG GACACTCGCT TTGTGCTCAA GATCACTGAC CATGGTTATG 
     3121 CAGAGTTCCT GGAGTCTCAC TGCTCTTTCA GGCCCCAGCC GGCCCCAGAA GAGCTTCTGT 
     3181 GGACGGCTCC TGAGCTGTTG CGGGGGCCTC GGGGGCCTTG GGGGCCTGGG AAGGCCACCT 
     3241 TCAAAGGTGA TGTTTTCAGC CTGGGCATCA TCCTGCAGGA GGTACTGACC CGAGACCCAC 
     3301 CCTACTGCTC CTGGGGACTC TCAGCAGAAG AGATCATCCG GAAGGTGGCA TCTCCCCCTC 
     3361 CTCTGTGCCG GCCTCTGGTG TCCCCTGACC AGGGTCCCCT GGAGTGCATC CAGTTGATGC 
     3421 AGCTGTGCTG GGAGGAAGCT CCAGATGACA GGCCAAGCTT GGACCAGATC TACACACAGT 
     3481 TCAAAAGCAT CAACCAAGGC AAGAAGACAA GTGTTGCTGA CTCCATGCTG CGGATGCTGG 
     3541 AGAAGTATTC CCAAAGCCTG GAAGGTCTGG TCCAGGAGCG GACTGAGGAG CTGGAACTGG 
     3601 AGAGGCGGAA GACAGAGAGG CTGCTCTCAC AGATGCTCCC CCCGTCTGTC GCCCATGCTC 
     3661 TAAAGATGGG AACAACTGTG GAGCCCGAGT ACTTTGACCA AGTCACCATC TATTTCAGTG 
     3721 ACATCGTGGG CTTTACCACC ATCTCAGCCT TGAGTGAGCC CATTGAGGTG GTGGGCTTCC 
     3781 TCAATGATCT CTACACGATG TTTGATGCTG TTCTAGACAG CCATGATGTG TATAAGGTAG 
     3841 AAACCATAGG GGATGCCTAC ATGGTGGCAT CTGGGCTCCC TCGGCGCAAC GGAAATCGGC 
     3901 ATGCTGCTGA GATTGCCAAC ATGGCTCTGG AGATCCTTAG CTACGCAGGC AACTTCCGGA 
     3961 TGAGGCATGC ACCTGATGTG CCCATCCGTG TCAGGGCCGG TCTGCATTCA GGGCCCTGTG 
     4021 TGGCAGGTGT TGTGGGTCTC ACCATGCCTC GGTACTGCCT CTTTGGGGAC ACCGTGAACA 
     4081 CTGCTTCCAG GATGGAATCC ACTGGACTGC CATACAGAAT CCACGTCAGC CGAAACACTG 
     4141 TCCAGGCCCT GCTTAGCCTT GACGAAGGCT ACAAAATTGA TGTCAGAGGT CAGACCGAAC 
     4201 TGAAGGGGAA GGGCTTGGAG GAGACTTACT GGCTGACAGG GAAGACAGGA TTTTGCAGAT 
     4261 CCCTCCCTAC ACCTCTGTCC ATCCAGCCTG GAGACCCATG GCAGGACCAT ATAAACCAAG 
     4321 AAATCCGGAC TGGCTTTGCA AAGCTCGCCA GAGTCTGCAC GCGTACGCGG CCGCTCGAGC 
     4381 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4441 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4501 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4561 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4621 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4681 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4741 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4801 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4861 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4921 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4981 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5041 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5101 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5161 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5221 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5281 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5341 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5401 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5461 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5521 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5581 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5641 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5701 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5761 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5821 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5881 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5941 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6001 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6061 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6121 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6181 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6241 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6301 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6361 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6421 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6481 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6541 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6601 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6661 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6721 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6781 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6841 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6901 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6961 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7021 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7081 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7141 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7201 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7261 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7321 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7381 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7441 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7501 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7561 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7621 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7681 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7741 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7801 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7861 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7921 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7981 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8041 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8101 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8161 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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