LOCUS       RR210552                7680 bp    DNA     circular  SYN 
DEFINITION  Pld2 (Myc-DDK-tagged ORF) - Rat phospholipase D2 (Pld2), (10 ug)
ACCESSION   NM_033299
VERSION     NM_033299.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3837..3928 
                     /label="Myc-DDK"  
     misc_feature    3958..4543 
                     /label="PolyA signal"  
     misc_feature    4015..4034 
                     /label="Restriction Enzyme"  
     misc_feature    4819..5491 
                     /label="ColE1"  
     misc_feature    5921..6715 
                     /label="Kan/Neo"  
     misc_feature    6750..7052 
                     /label="SV40 ori"  
     misc_feature    7138..7615 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3819..3825 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3819 
                     /label="Pld2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGTAACC CAGACGGACC TCTTTCCCTA TGGGGACTAC CTGAACTCCA 
     1081 GCCAGTTGCA CATGGAGCCA GATGAGGTTG ACACTCTGAA GGAAGGAGAG GATCCAGCTG 
     1141 ATCGAATGCA TCCCTTTCTG GCCATCTATG ACCTTCAGCC TCTGAGAGCA CACCCCTTGG 
     1201 TGTTTGCTCC TGGGGTCCCT GTTATAGCCC AGGTGGTAGG CACCGAAAGA TACACCAGCG 
     1261 GATCCAAGGT GGGAACCTGT ACTCTTTATT CTGTTCGATT GACCCATGGC GACTTTACCT 
     1321 GGACAACCAA GAAGAAATTC CGACACTTTC AGGAGCTGCA TCGGGACCTC CAGAGACACA 
     1381 AAGTCTTGAT GAGTCTGCTC CCTCTGGCTC GCTTTGCTGC GGCCCATTCT CCAGCCCGAG 
     1441 AGGCAGCCAA TGAGAATATT CCCTCCCTAC CCCGAGGAGG TTCCGAGGGC TCTGCCAGAC 
     1501 ACACAGCCAG CAAGCAGAAG TACCTGGAAA ATTACCTCAA CCGCCTCCTG ACCATGTCTT 
     1561 TCTATCGAAA TTACCATGCC ATGACAGAAT TTCTGGAAGT CAGTCAACTT TCCTTTATCC 
     1621 CAGACCTTGG CTCCAAAGGA CTGGAAGGGG TGATCCGGAA GCGCTCAGGT GGGCATCGAG 
     1681 TTCCTGGCTT CACCTGCTGT GGTCGAGACC AAGTTTGTTA TCGATGGTCC AAGAGGTGGC 
     1741 TGGTGGTGAA GGACTCCTTC CTGCTGTACA TGCGTCCAGA GACTGGCGCC ATCTCATTTG 
     1801 TTCAACTTTT TGACCCTGGC TTTGAGGTCC AGGTGGGGAA AAGGAGCACA GAGGCACGGT 
     1861 ATGGGGTGAG GATCGACACC TCCCACAGGT CCCTGATCCT CAAATGCAGC AGCTACCGGC 
     1921 AGGCACGGTG GTGGGGCCAG GAGATCACGG AGCTGGCACA GGGTCCGGGC AGAGATTTTC 
     1981 TACAGCTACA CCAGCATGAC AGCTATGCCC CACCCCGGCC TGGCACCCTG GCCCGGTGGT 
     2041 TTGTGAATGG GGCAGGTTAC TTTGCTGCTG TGGCAGATGC CATCCTCCGA GCTCGAGAGG 
     2101 AGATTTTCAT CACAGACTGG TGGCTGAGTC CTGAGATTTA CCTGAAGCGT CCAGCCCACT 
     2161 CAGATGACTG GAGACTGGAC ATTATGCTCA AGAGGAAGGC GGAAGAAGGT GTCCGTGTTT 
     2221 CCATACTGCT GTTTAAGGAA GTGGAACTGG CCTTGGGCAT CAACAGTGGC TATAGCAAGA 
     2281 GGACACTGAT GCTACTGCAC CCCAACATAA AGGTGATGCG ACACCCAGAC CTTGTAACAT 
     2341 TGTGGGCTCA TCATGAGAAG CTCCTGGTGG TAGACCAAGC GGTGGCATTC TTGGGTGGGC 
     2401 TGGACCTTGC CTATGGCCGC TGGGATGATG TGCAGTACCG ACTGACTGAC CTGGGGGACC 
     2461 CCTCCGAATC TGCAGATTCA CAGACTCCCA CGCCAGGTTC AGATCCTGCA GCCACTCCAG 
     2521 ACCTCTCGCA TAATCACTTC TTCTGGCTGG GAAAAGACTA CAGCAACCTC ATTACCAAGG 
     2581 ACTGGGTACA GCTGGACCGG CCTTTTGAAG ATTTCATCGA CAGGGAGACC ACACCCAGAA 
     2641 TGCCATGGAG GGATGTTGGA GTGGTTGTAC ATGGAGTAGC TGCCCGGGAC CTTGCCCGGC 
     2701 ACTTCATCCA GCGCTGGAAC TTCACCAAGA CCATCAAGGC CAGATACAAG ATACCTCAGT 
     2761 ACCCCTACCT GCTGCCTAAG TCCGCCAGCA CTGCAAACCA TCTTCCCTTC ATAATCCCAG 
     2821 GCGCGCAGTG CGCCACTGTG CAGGTCTTGC GGTCTGTGGA TCGATGGTCA GCAGGGACTT 
     2881 TGGAGAGCTC CATCCTCAAT GCCTACCTAC ATACCATCCG AGAGAGCCAG CACTTTCTCT 
     2941 ACATTGAGAA TCAGTTCTTC ATCAGCTGCT CAGATGGGCG TACAGTTCTG AACAAGGTGG 
     3001 GCGATGAGAT TGTGGACAGG ATCCTAAAGG CTCATGAACA GGGGCAGTGT TTCCGAGTCT 
     3061 ACGTGCTTCT GCCTTTGCTC CCTGGCTTTG AGGGGGACAT CTCCACAGGG GGTGGCAACT 
     3121 CCATCCAGGC CATTCTGCAC TTCACCTACA GGACCCTGTG TCGTGGGGAA TATTCAATCT 
     3181 TACATCGTCT CAAAGCAGCC ATGGGGACAG CATGGCGGGA TTACATGTCC ATCTGTGGGC 
     3241 TTCGCACACA TGGAGAGCTG GGCGGGCACC CGATCTCCGA GCTCATCTAT ATCCACAGCA 
     3301 AGTTGCTCAT TGCAGATGAC AGAACAGTCA TCATCGGCTC TGCAAACATC AACGACAGGA 
     3361 GCTTGCTGGG GAAGCGCGAC AGTGAGCTAG CCATACTGAT CGAGGACACA GAAATGGAGC 
     3421 CATCCCTCAT GGATGGGGTG GAGTACCAGG CAGGCAGATT TGCCTTGAGT CTGCGGAAGC 
     3481 ACTGTTTCAG TGTCATTCTT GGGGCAAATA CCTGGCCAGA CCTGGATCTC CGAGACCCTG 
     3541 TCTGTGATGA CTTCTTCCAG CTGTGGCAGG AAACAGCGGA GAACAATGCC ACCATCTATG 
     3601 AGCAGATCTT CCGCTGCCTG CCGTCCAATG CTACTCGTTC CCTGCGGGCT CTCCGGGAGT 
     3661 ACGTGGCTGT GGAGTCCTTG GCTACAGTCA GCCCTTCTTT GGCTCAGTCT GAGCTTGCCC 
     3721 ACATCCGGGG CCACTTAGTT CACTTCCCCC TCAAGTTTCT GGAGGATGAG TCCTTGTTGC 
     3781 CTCCACTGGG GAGCAAAGAG GGGATGATAC CTTTAGAAGT GTGGACAACG CGTACGCGGC 
     3841 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3901 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3961 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4021 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4081 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4141 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4201 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4261 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4321 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4381 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4441 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4501 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4561 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4621 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4681 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4741 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4801 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4861 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4921 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4981 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5041 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5101 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5161 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5221 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5281 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5341 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5401 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5461 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5521 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5581 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5641 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5701 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5761 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5821 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5881 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5941 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6001 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6061 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6121 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6181 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6241 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6301 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6361 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6421 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6481 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6541 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6601 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6661 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6721 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6781 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6841 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6901 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6961 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7021 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7081 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7141 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7201 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7261 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7321 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7381 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7441 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7501 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7561 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7621 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//