LOCUS       RR210483                8544 bp    DNA     circular  SYN 
DEFINITION  Vcpip1 (Myc-DDK-tagged ORF) - Rat valosin containing protein (p97)/p47 complex interacting protein 1 (Vcpip1), (10 ug)
ACCESSION   NM_176857
VERSION     NM_176857.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4701..4792 
                     /label="Myc-DDK"  
     misc_feature    4822..5407 
                     /label="PolyA signal"  
     misc_feature    4879..4898 
                     /label="Restriction Enzyme"  
     misc_feature    5683..6355 
                     /label="ColE1"  
     misc_feature    6785..7579 
                     /label="Kan/Neo"  
     misc_feature    7614..7916 
                     /label="SV40 ori"  
     misc_feature    8002..8479 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4683..4689 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4683 
                     /label="Vcpip1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCAGCCG CCGCCGCCTC CTCCGCTGCC GCCGCCGCCG CCTCCCCCCG 
     1081 AGGCTCCGCA GACTTCGTCG TCCCTGGCGG CGGCGGCTAC TCCGGGGGGC CTTTCGAAAC 
     1141 GAAGGGACCG GAGAATCCTT TCCGGGAGCT GCCCGGATCC CAAGTGCCAG GCGCGGCTCT 
     1201 TCTTCCCGGC CTCAGGTTCT GTCAGCATCG AGTGTACCGA GTGCGGACAG CGGCACGAGC 
     1261 AGCAACAGCT GCTGGGAGTG GAGGAGGTGA CCGACCCGGA CGTAGTGCTG CACAATCTGC 
     1321 TGCGGAACGC GCTACTCGGG GTGACGGGGG CTCCCAAGAA GAACACGGAA CTGGTAAAGG 
     1381 TGATGGGCCT CTCCAACTAC CACTGCAAAT TATTGTCGCC CATATTGGCA CGCTATGGAA 
     1441 TGGACAAACA AACGGGCCGC GCCAAGCTTC TCCGGGACAT GAACCAAGGC GAACTGTTCG 
     1501 ATTGCGCGTT ACTCGGTGAC CGGGCCTTCC TCATCGAACC AGAGCACGTC AACACAGTGG 
     1561 GCTATGGCAA GGACCGATCT GGAAGCCTCC TATATTTGCA TGACACTCTC GAGGATATCA 
     1621 AACGGGCCAA TAAAAGTCAA GAATGCCTTA TTCCAGTGCA TGTGGATGGG GATGGACACT 
     1681 GCCTAGTGCA TGCTGTGTCT AGGGCTCTAG TAGGCCGAGA GCTCTTCTGG CATGCCCTGA 
     1741 GGGAGAATCT GAAGCAGCAC TTTCAGCAGC ACCTAGCCCG ATATCAAGCC CTGTTTCATG 
     1801 ACTTTATTGA TGCTGCAGAG TGGGAGGACA TTATCAATGA ATGTGACCCT CTGTTTGTGC 
     1861 CACCTGAAGG TGTCCCCTTA GGCCTGAGGA ATATCCACAT ATTTGGCCTT GCCAATGTGT 
     1921 TACATCGTCC CATAATTCTC TTAGATTCCC TCAGTGGCAT GAGAAGCTCT GGTGATTATT 
     1981 CAGCCACCTT TCTACCTGGG CTCATACCTG CAGAGAAGTG CACTGGGAGA GATGGTCATT 
     2041 TGAACAAGCC AATCTGTATT GCGTGGAGTA GCTCTGGTAG AAACCATTAT ATTCCCTTGG 
     2101 TAGGCATAAA GGGGGCTGCC TTGCCCAAAC TACCCATGAA TTTGCTACCT AAAGCATGGG 
     2161 GTGTGCCTCA GGACCTTATT AAAAAGTACA TCAAGCTTGA GGAGGATGGT GGTTGTGTTA 
     2221 TTGGAGGAGA CAGAAGTTTG CAGGATAAGT ACTTACTTAG GTTGGTTGCT GCTATGGAGG 
     2281 AAGTCTTTAT GGACAAACAT GGTATCCATC CTAGTTTGGT TGCTGATGTA CATCAGTATT 
     2341 TCTACAGAAG GACTGGGGTG ATAGGAGTTC AGCCTGAGGA AGTTACAGCA GCTGCTAAAA 
     2401 AAGCAGTAAT GGATAATCGC CTTCACAAGT GTTTGCTTTG TGGTGCCCTT TCTGAACTTC 
     2461 ATGTCCCTCC TGAGTGGTTG GCTCCAGGAG GAAAACTGTA TAACCTGGCA AAAAGTACTC 
     2521 ATGGACAGCT GAGGCCTGAC AAAAATTACA GCTTTCCTTT AAACAATTTG GTTTGTTCAT 
     2581 ATGATCCAGT GAAAGATGTT CTGTTACCAG ACTATGGATT GAGTAATCTA ACAGCTTGTA 
     2641 ATTGGTGCCA TGGCACATCT GTGCGAAGAG TCAGAGGCGA TGGCTCTATT GTATATTTGG 
     2701 ATGGAGACAG AACTAATTCT AGGTCTACTG GTGGCAAATG TGGTTGTGGA TTCAAACACT 
     2761 TTTGGGAAGG TAAAGAATAT GACAACCTTC CAGAAGCTTT TCCTATCACT TTGGAGTGGG 
     2821 GAGGAAGAGT AGTCAGAGAA ACAGTATATT GGTTCCAGTA TGAAAGTGAT CCATCTTTGA 
     2881 ATAGTAATGT TTATGATGTT GCAATGAAAC TTGTTACCAA GCACTTTCCA GGTGAATTTG 
     2941 GGAGTGAGAT CCTAGTTCAG AAAGTTGTCC ACACTATATT GCATCAGACT GCAAAAAAAA 
     3001 ATCCTGACGA TTACACTCCT GTAAATATAG ATGGTGCTCA TGCTCAGAGA ATTGGAGATG 
     3061 TGCAAGGACA AGAATTGGAG TCTCAGCTAC CAACTAAAAT TATTCTTACT GGACAGAAAA 
     3121 CAAAAACTTT GCACAAGGAA GAATTAAACA TGAGTAAAAC TGAAAGAACT ATTCAACAGA 
     3181 ACATCACAGA ACAAGCTTCT GTGATGCAGA AACGGAAAAC AGAGAAATTA AAACAAGAGC 
     3241 AAAAGGGGCA ACCCAGAACT GTTTCTCCAA GTACTATTCG TGATGGGCCT TCATCTGCAC 
     3301 CTGCCACCCC CACGAAGGCT CCCTACTCAC CTACCACTTC TAAGGAGAAG AAGATTCGCA 
     3361 TAACAACTAA TGATGGACGG CAGTCCATGG TTACCCTTAA GTCTTCAACA ACCTTTTTTG 
     3421 AACTTCAGGA AAGTATAGCC AGAGAGTTCA ACATTCCTCC ATATTTACAG TGTATTCGAT 
     3481 ATGGTTTTCC TCCTAAAGAG TTAATGCCAC CCCAAGCAGG AATGGAAAAG GAGCCAGTTC 
     3541 CTTTGCAGCA TGGTGACAGA ATTACCATAG AGATCTTAAA AGGCAAAGCA GAAGGTGGTC 
     3601 CATCCACTGC TGCACACTCA GCCCACACTG TGAGACAAGA AGAGATTGCT GTTACTGGCA 
     3661 AGCTGTCCTC CAAGGAACTT CAGGAGCAAG CTGACAAAGA AATGTATTCC TTGTGTCTTT 
     3721 TAGCAACATT AATGGGAGAA GACGTGTGGT CTTATGCAAA GGGACTTCCT CACATGTTCC 
     3781 AGCAGGGTGG TGTATTCTAC AATATTATGA AGAAAACTAT GGGCATGGCT GATGGCAAAC 
     3841 ATTGTACTTT TCCACATCTA CCTGGCAAAA CCTTTGTTTA TAATGCTTCT GAAGACAGAC 
     3901 TGGAGTTGTG TGTCGATGCC GCAGGACATT TCCCCATTGG TCCTGATGTT GAAGATTTAG 
     3961 TTAAAGAGGC TGTAAGTCAG GTTCGAGCAG AGGCTACTAC AAGAAGTAGG GAATCAAGCC 
     4021 CTTCACATGG GTTATTAAAA CTAGGTAGTG GTGGAGTAGT GAAAAAGAAG TCTGAGCAAC 
     4081 TTCACAATGT AACTGCCTTT CAGGGGAAGG GCCATTCTCT AGGAACTGCA TCCAGTAACC 
     4141 CGCACATGGA TCCCAGAGCT AGGGAAACTC TGGCTGTAAG AAAGCATAAT ACAGGGACAG 
     4201 ATTTTAGTAA TAGTTCCATT AAAACAGAGC CTCCTGTGTT CACAGCTGCT TCTAGTAATA 
     4261 GTGAGCTTAT TCGAATAGCT CCTGGAGTGG TAACAATGAG AGATGGTAGG CAGATTGATC 
     4321 CCGATGTGGT TGAGGCCCAG CGAAAAAAAT TGCAGGAAAT GGTTTCTTCT ATTCAGGCAT 
     4381 CAATGGACAA GCACTTGCGG GATCAAAGTA CAGAGCAAAC ACCATCTGAT CTTTCTCAAA 
     4441 GAAAAGTAGA AGCTGTGAGT TCTTCTGTGA GGCCTGGGAA TCTTCAGACT GGCTTGCCTG 
     4501 AATCTTTTTC TTTAACTGGT GGCACTGAGA ATTTGAATAC TGAAACAACT GATAGTCGTG 
     4561 TAGCAGATGT ACTGGGAGCA GCATTTGCCA CAAGGTCAAA AGCACAAAAA GAAAATTCCA 
     4621 TGGAGGAACC TGAAGAGATG GATAGTCAAG ATGCTGAGAC AACTAACACA ACTGAGCCGA 
     4681 TGGATCACTC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4741 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4801 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4861 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4921 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4981 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5041 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5101 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5161 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5221 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5281 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5341 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5401 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5461 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5521 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5581 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5641 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5701 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5761 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5821 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5881 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5941 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6001 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6061 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6121 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6181 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6241 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6301 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6361 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6421 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6481 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6541 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6601 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6661 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6721 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6781 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6841 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6901 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6961 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7021 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7081 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7141 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7201 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7261 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7321 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7381 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7441 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7501 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7561 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7621 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7681 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7741 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7801 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7861 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7921 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7981 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8041 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8101 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8161 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8221 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8281 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8341 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8401 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8461 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8521 TAACAAAAAT TTAACGCGAA TTTT
//