LOCUS       RR210421                7275 bp    DNA     circular  SYN 
DEFINITION  Rin1 (Myc-DDK-tagged ORF) - Rat Ras and Rab interactor 1 (Rin1), (10 ug)
ACCESSION   NM_139038
VERSION     NM_139038.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3432..3523 
                     /label="Myc-DDK"  
     misc_feature    3553..4138 
                     /label="PolyA signal"  
     misc_feature    3610..3629 
                     /label="Restriction Enzyme"  
     misc_feature    4414..5086 
                     /label="ColE1"  
     misc_feature    5516..6310 
                     /label="Kan/Neo"  
     misc_feature    6345..6647 
                     /label="SV40 ori"  
     misc_feature    6733..7210 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3414..3420 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3414 
                     /label="Rin1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGCACGCCT GTGCCTTGGG GGTTGGATTC TCTTCCTGAG TCCCAGGAGA 
     1081 AGCTGAAGGA GCTCCCAGCC ATGGAAGACC CTGGTGAGAC CGAAGCACAC CCTCTGGGAG 
     1141 CCACCAGCCT GAACTTTGTA CCTGGGTACC AACAGGAAGA AAAGCCATCT CCAGACCCAC 
     1201 TGTATGACAC ACCTGATGCC AGAGGGGTTC AGGCAGGCGG GTCCCAACAA CCAGCACGCA 
     1261 CAGTGAGCTT GCGGGAGCGG CTGCTGATCA CCCGGCCTGT GTGGTTACAG CTGCGGGCCA 
     1321 ACGCTGCAGC CGCACTGCAC GTGCTGAGGA CAGAGCCTCC GGGGACCTTC CTGGTGCGGA 
     1381 AATCTAACAC TCGCCAGTGC CAGACTCTAT GTGTGCGGCT GCCAGAAGCT AGTGGCCCCT 
     1441 CTTTTGTCTC CAGCCACTAT CTCCAAGAGA GTCCTGGCGG CATCTCTTTG GAGGGCTCAG 
     1501 AGCTCACATT CCCGGACCTG GTCCAGCTCA TCTGTGCATA CTGTCATACC AGGGACATTC 
     1561 TTCTCCTCCC TCTCCCGCTC CCCAGAGCCA TTCATCAGGC AGCCACCCAC AAAGAGCTGG 
     1621 AGGCCATCTC CCATCTGGGC ATGGAGTTCT GGAGTTCCTC TCTCAACACC AAGAACCAAC 
     1681 AGAGACCTTC AGAAGCTCCG CAGATACCCC GGCTGAAGGC TCGCTCCCCT CAAGAGCTGG 
     1741 ATCAGGGCAC TGGTGCAGCC CTCTGCTTCT TCAACCCCCT CTTCCCCGGG GATCTGGGCC 
     1801 CCACCAAACG TGAGAAATTC AAGAGGAGTT TCAAAGTGCG TGTATCCACA GAGACCTCCA 
     1861 GCCCTCTGTC TCCACCTGCT GTGCCGCCTC CTCCAGTCCC CGTTCTGCCA GGGACATCTT 
     1921 CCAGCCAAAC GGAAAGACTG CCCCCTCGAC AGCTGCTGCA GAGGGAGAGC TCAGTGGGAT 
     1981 ACCGTGTGCC AGGAAGTGCT TCTGGTCCTA GTCTTCCTCC CCTACCTTCA CTTCAGGAGG 
     2041 TGGACTGCTG CTCCCCCAGC AGCTCAGAGG AGGAGGGTTC TTCAGGAAGC CCTACAACCT 
     2101 CCCCACGCCT AAGCCGCCCA AGGCACCGGC GGCCTCTGCT TCGGTCCATG AGTTCTGCTT 
     2161 TCTGCTCTCT GCTGGCACCA GAGAGGCAGG TGGGCCGGGC AGCTACAATG CTAATGCAAA 
     2221 ACCGATACAC AGCTGTGGGT CAGCTAGTGC AGGACCTATT GACCCAGGTT CGTGTTGGTC 
     2281 CTGAGTCCCG GGAGTTGCAG GGCATCCGAC AGGCCCTGAG TCGGGCACGG GCCATGCTGA 
     2341 GTGCAGAGCT GGGCCCTGAG AAACTGCTAC CACCAGAGCG GCTGGAATTG GTCCTGGAGA 
     2401 AATCCCTGCA TCGCTCTGTT CTCAAGCCTC TTCGACCCAT TCTAGCTGCC CGCCTGCGGC 
     2461 GCCGACTTTC CACAGACGGT TCCCTTGGCC GCCTAGCTGA GGGCTTCCGC CTGGCCCGGA 
     2521 CCCAGGGGCC TGGAGCCTTC GGGTCCCACC TGAACCTTTC TTCCCCAGTG GAGATAGAAC 
     2581 CAGTGCGTCA GAAGCTGCTG CAGCTCCTTC GTGCCTACTC GCCTAGTGCC CAGATCAAGT 
     2641 GGCTCCTGCA GGCCTGCAAG TTGCTATACA CAGCCCTGAA AACCCAGGCA GGGGAGAATG 
     2701 CAGGTGCTGA TGAGTTCCTG CCTCTCTTGA GCCTTGTCTT GGCCCAGTGC GACCTTCCTG 
     2761 ACCTCCTGTT AGAAGCTGAG TATATGTCAG AGCTACTGGA ACCCACATTG CTCACTGGGG 
     2821 AGGGCGGCTA CTACCTGACC AGCCTCTCAG CCAGCCTGGC CTTGTTGAGT GGCCTAAGCC 
     2881 AGGCCCACGC ACTGCCACTC AGCCCTGCCC AGGAGCTCCA ACGTTCTCTG GCCCTCTGGG 
     2941 AACAGCGCCG CCTGCCGGCC ACCCACAACT TCCAGCACCT CCTCCGAGTA GCCTACCAGG 
     3001 ACCCCAGCAC AGGTTGCACC TCCAAGACCT TGGCTGTGCC CCCGGGGTCT TCGATTGCCA 
     3061 CCCTGAGTCA ACTCTGTGCC ACCAAGTTCC GAGTGACCCA ACCGGATGCA TTTGGCCTCT 
     3121 TTCTCTACAA GGACCAGGGC TACCATCGCC TGCCCCCTGA AGCCCTAGTC CACAGGCTTC 
     3181 CCACAACTGG TTACCTCATC TACCGCCGGG CAGAGCGCCC CGAGACCCAG AGGGCTGCAA 
     3241 CAGAGAAGAC CAAGACAGGA AATGAGAGGC CAGAGCGAGG AGCATGGGAG GAGGAGAAAG 
     3301 GGGGTCTAAA TGGTGAAGGG AAATCTGAGA TAGCTGTTGA CCAAGAAGGC AAGGATCAGG 
     3361 CCAGAGGAGG TCACATGCAG CTAGAGGAAC AAAAGGCAGA GGGATGCCCG GCTTTAGAAG 
     3421 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3481 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3541 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3601 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3661 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3721 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3781 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3841 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3901 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3961 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4021 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4081 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4141 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4201 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4261 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4321 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4381 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4441 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4501 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4561 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4621 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4681 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4741 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4801 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4861 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4921 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     4981 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5041 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5101 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5161 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5221 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5281 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5341 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5401 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5461 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5521 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5581 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5641 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5701 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5761 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5821 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5881 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     5941 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6001 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6061 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6121 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6181 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6241 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6301 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6361 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6421 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6481 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6541 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6601 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6661 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6721 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6781 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6841 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6901 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     6961 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7021 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7081 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7141 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7201 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7261 TTTAACGCGA ATTTT
//