LOCUS       RR210349                6659 bp    DNA     circular  SYN 
DEFINITION  Gpr162 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 162 (Gpr162), (10 ug)
ACCESSION   NM_001108646
VERSION     NM_001108646.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2816..2907 
                     /label="Myc-DDK"  
     misc_feature    2937..3522 
                     /label="PolyA signal"  
     misc_feature    2994..3013 
                     /label="Restriction Enzyme"  
     misc_feature    3798..4470 
                     /label="ColE1"  
     misc_feature    4900..5694 
                     /label="Kan/Neo"  
     misc_feature    5729..6031 
                     /label="SV40 ori"  
     misc_feature    6117..6594 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2798..2804 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2798 
                     /label="Gpr162"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCGGGGT GGGTTGGGGG CAGAAGAGGC CTCCCTACGC TCAAACGCAT 
     1081 TGTCCTGGCT GGCCTGCGGG CTGCTGGCCC TGCTGGCCAA CGCCTGGATC ATCCTCAGCA 
     1141 TCTCGGCCAA ACAGCAGAAG CACAAGCCAC TGGAGCTGCT GCTCTGCTTC CTGGCGGGCA 
     1201 CACATATACT CATGGCCGCT GTGCCCCTTA CCACCTTCGC TGTGGTACAG CTCCGGCGCC 
     1261 AGGCCTCCTC TGACTATGAC TGGAACGAGA GCATCTGCAA GGTCTTTGTG TCCACCTACT 
     1321 ACACGCTGGC CCTGGCCACC TGCTTCACGG TCGCCTCGCT CTCCTACCAC CGCATGTGGA 
     1381 TGGTGCGCTG GCCCGTTAAC TACCGCCTCA GCAACGCCAA GAAGCAGGCA CTGCACGCCG 
     1441 TCATGGGCAT CTGGATGGTC AGCTTTATCC TGTCCACGCT GCCCTCCATC GGCTGGCACA 
     1501 ACAACGGGGA GCGCTACTAC GCCCGAGGCT GCCAGTTCAT AGTCTCCAAG ATTGGCCTGG 
     1561 GCTTTGGTGT CTGCTTCAGC CTCTTGCTCC TTGGGGGCAT TGTCATGGGG TTGGTCTGTG 
     1621 TGGCCATCAC GTTCTATCAG ACACTGTGGG CCCGGCCCCG GAGGGCTCGG CAAGCCCGGA 
     1681 GAGCGGGGGG CAGCGTGGGA GCCAAGGCAG GTGGGCTGGG GGGCTTGGGT ACCCGGCCAG 
     1741 CTTTTGAGGT GCCAGCCATC GTGGTGGAGG ACACCCGAGG GAAGCGGCGG TCCTCGCTGG 
     1801 ATGGCTCTGA ATCTGCCAAG ACATCCTTGC AGGTCACCAA CTTGGTCAGC GCCATCGTCT 
     1861 TTCTCTATGA CTCGCTCACG GGGGTGCCCA TCTTGGTGGT GAGCTTCTTC TCCTTGAAGT 
     1921 CAGACTCAGC CCCGCCATGG ATGGTCCTGG CTGTGCTGTG GTGCTCCATG GCACAGACGT 
     1981 TGCTCCTGCC CTCCTTCATC TGGTCCTGTG AGCGCTACCG TGCAGATGTG CGCACAGTGT 
     2041 GGGAGCAGTG TGTGGCCATC ATGTCCGAGG ATGACGGTGA TGACGATGGC GCCTGTGATG 
     2101 ACTACACAGA CGGCCGCGTG TGCAAAATTC GCTTTGATGC TAATGGAGCC ACAGGGTCAG 
     2161 GGAGTCGGGA CCCCACCCAG GTGAAACTGC TGCCGGGTCG GCACATGCTC TTCCCACCCC 
     2221 TTGAGAGAGT ACACTATTTA CAGGTTCCTT TGTCCCGCCG GTTATCCCAT GATGAGACCA 
     2281 ACGTCTTTTC TACTCCTCGG GCACCAGGCT CCTTCCTGCA CAAGTGGTCA TCGTCTGATG 
     2341 ATATCCGGAT CCTCCCAGCC CAGAGCAGAG CCCTTGGAGG TCCTCCAGAA TACCCGGGAC 
     2401 AGAGACTCAG GATAGAGGAT GAGGAGGACG AGGAAGAGGC TGAAGGTGGG GGCTTAGCCA 
     2461 GCCTTCGACA GTTTCTAGAA AGTGGGGTTC TGGGGTCAGG TGGGGGACCC CCACGAGGTC 
     2521 CTGGTTTTTT CCGGGAGGAG ATCACCACCT TCATCGATGA GACACCTCTG CCTTCTCCAA 
     2581 CTGCATCACC AGGACCCTCT CCTCGTAGGC CTAGGCCGCT GGGCTTTTCA CCTCGACGAC 
     2641 TCTCCCTTGG GTCTCCTGAG AGCCGAGCTG TTGGACTTCC TTTGGGGTTA AGTGCAGGAA 
     2701 GACGCTGTTC CCTTACAGGG AGTGAAGGGA GTTCAAGGGC TTGGGGAAGA CCCTGGGGGC 
     2761 CAGGCAACCC CATCTTCCCC CAGTTGACCC TGCCCAGTTG ACCCTGACGC GTACGCGGCC 
     2821 GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA 
     2881 GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC 
     2941 CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC 
     3001 CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT 
     3061 CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA 
     3121 CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT 
     3181 GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT 
     3241 TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC 
     3301 GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC 
     3361 CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC 
     3421 TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC 
     3481 ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT 
     3541 CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC 
     3601 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 
     3661 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 
     3721 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 
     3781 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 
     3841 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 
     3901 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 
     3961 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 
     4021 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 
     4081 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 
     4141 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 
     4201 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 
     4261 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 
     4321 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 
     4381 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 
     4441 TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG 
     4501 AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG 
     4561 TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC 
     4621 CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC 
     4681 ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG 
     4741 TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG 
     4801 AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT 
     4861 GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT 
     4921 CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA 
     4981 CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG 
     5041 CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC 
     5101 GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT 
     5161 CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT 
     5221 GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT 
     5281 CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC 
     5341 GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA 
     5401 GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT 
     5461 CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT 
     5521 CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT 
     5581 GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT 
     5641 AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA 
     5701 TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA 
     5761 AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA 
     5821 TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG 
     5881 GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT 
     5941 TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG 
     6001 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC 
     6061 TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT 
     6121 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     6181 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     6241 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     6301 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     6361 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     6421 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     6481 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     6541 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     6601 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//