LOCUS       RR210325                7956 bp    DNA     circular  SYN 
DEFINITION  Dpyd (Myc-DDK-tagged ORF) - Rat dihydropyrimidine dehydrogenase (Dpyd), (10 ug)
ACCESSION   NM_031027
VERSION     NM_031027.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4113..4204 
                     /label="Myc-DDK"  
     misc_feature    4234..4819 
                     /label="PolyA signal"  
     misc_feature    4291..4310 
                     /label="Restriction Enzyme"  
     misc_feature    5095..5767 
                     /label="ColE1"  
     misc_feature    6197..6991 
                     /label="Kan/Neo"  
     misc_feature    7026..7328 
                     /label="SV40 ori"  
     misc_feature    7414..7891 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4095..4101 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4095 
                     /label="Dpyd"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGTGTG CTGAGCAGGG ACGCGCCGGA CATCGAGAGT ATCCTGGCTT 
     1081 TAAATCCTCG AATACAAGCT CATGCGACTC TTCGTTCCAC CATGGCCAAG AAACTAGACA 
     1141 AGAAACATTG GAAAAGGAAC ACTGATAAGA ACTGCTTTAT CTGTGAGAAG CTGGAGAATA 
     1201 ATTTTGATGA CATCAAGCAC ACGACTCTTG GTGAGCGAGG GGCTCTCCGA GAAGCAGTGA 
     1261 GATGCTTGAA ATGTGCAGAT GCTCCCTGCC AGAAGAGTTG TCCCACGTCT CTTGACATTA 
     1321 AGTCATTCAT CACAAGTATC GCCAACAAGA ACTACTATGG TGCAGCTAAG CTGATTTTTT 
     1381 CCGATAATCC TCTTGGTCTT ACTTGCGGAA TGGTTTGTCC AACATCTGAC CTCTGTGTCG 
     1441 GAGGATGCAA CTTACATGCT ACTGAAGAGG GGCCAATTAA TATTGGTGGA CTGCAGCAGT 
     1501 TTGCTACCGA GGTGTTCAAA GCGATGAACA TCCCACAGAT CAGAAGCCCG TTGCTGCCTC 
     1561 CTCCGGAACA TATGCCGGAA GCTTACTCAG CAAAAATTGC GCTGTTTGGA GCTGGGCCTG 
     1621 CGAGTATAAG CTGTGCCTCC TTTCTGGCTC GACTGGGCTA TTCCGACATC ACCATATTTG 
     1681 AAAAGCAAGA ATACGTTGGT GGCTTAAGCA CTTCTGAAAT CCCTCAGTTT CGGCTCCCAT 
     1741 ATGATGTCGT GAATTTTGAG ATTGAGCTCA TGAAGGACCT TGGTGTCAAG ATAATTTGTG 
     1801 GTAAAAGCAT TTCCACAGAT GAAATGACTC TTAGTACTTT GAAAGAAAAT GGCTACAAAG 
     1861 CTGCTTTTAT TGGAATAGGT TTGCCAGAAC CCAAAAAGGA CCATATTTTC CAAGGCTTGA 
     1921 CACAAGTCCA GGGATTTTAC ACATCCAAAG ACTTTTTGCC ACTTGTCGCC AAAGGTAGCA 
     1981 AACCAGGAAT GTGCGCCTGT CACTCTCCAT TGCCATCCGT GAGGGGAGCC GTGATTGTAC 
     2041 TCGGAGCTGG GGACACTGCG TTTGACTGTG CAACATCCGC TCTGCGCTGC GGAGCACGTC 
     2101 GCGTGTTCAT CGTCTTCAGA AAGGGCTTTG CTAATATTCG AGCTGTTCCA GAGGAGATGG 
     2161 AGCTTGCTAA GGAAGAGAAA TGTGAATTTT TGCCTTTCCT TTCCCCACGG AAGGTTATAG 
     2221 TCAAAGATGG AAAGATTGTA GGAATGCAGT TTGTTCGAAC TGAGCAGGAT GAAACCGGAA 
     2281 ACTGGGTCGA AGATGAAGAG CAGATAGTGC GTTTGAAGGC TGATGTGGTT ATTAGCCCCT 
     2341 TTGGATCTGT CTTGGATGAT CCCAAAGTGA TAGAAGCATT GAGTCCCATC AAGTTTAACA 
     2401 GATGGGGTCT CCCTGAAGTA AACCCAGAAA CCATGCAAAC CAGTGAGCCA TGGGTATTTG 
     2461 CAGGTGGTGA TGTTGTGGGT ATGGCTAACA CCACAGTGGA ATCTGTCAAC GATGGAAAAC 
     2521 AGGCTTCATG GTACATTCAC GAGTACATAC AGGCACAATA TGGAGCCTTG GTGCCTTCCC 
     2581 AGCCTACACT GCCCCTGTTT TACACTCCTG TTGACCTCGT GGACATCAGT GTGGAAATGG 
     2641 CAGGGTTGAG GTTCCCCAAT CCCTTTGGCC TTGCCAGTGC GACACCAGCC ACTAGCACAC 
     2701 CAATGATTCG AAGGGCCTTT GAAGCAGGAT GGGGTTTTGC TTTGACCAAA ACTTTCTCTC 
     2761 TTGATAAGGA CATCGTGACA AACGTCTCAC CCAGAATCAT CCGAGGGACC ACTTCTGGCC 
     2821 CCTTGTATGG CCCTGGACAA AGCTCTTTCC TCAACATTGA GCTCATCAGT GAGAAAACAG 
     2881 CTGCATATTG GTGTCACAGT GTCACCGAAC TAAAGGCTGA CTTCCCGGAC AACATCCTGA 
     2941 TCGCCAGCAT CATGTGCAGT TACAACAAAA ATGACTGGAT GGAACTCTCC AAAATGGCTG 
     3001 AGGCTTCTGG AGCAGATGCC CTGGAGTTAA ATTTATCCTG TCCACATGGC ATGGGGGAGA 
     3061 GAGGAATGGG TCTGGCTTGT GGGCAGGATC CAGAGCTGGT GAGGAACATC TGTCGCTGGG 
     3121 TGAGACAATC TGTTCGGGTT CCATTTTTTG CCAAGTTGAC CCCAAATGTC ACTGATATTG 
     3181 TAAGCATCGC AAGAGCAGCA AAGGAAGGTG GTGCAGATGG CGTTACAGCC ACCAACACTG 
     3241 TCTCAGGCCT GATGGGACTG AAAGCTGATG GTTCACCCTG GCCTTCGGTG GGCAGTGGAA 
     3301 AGAGGACTAC ATATGGAGGA GTATCAGGAA CTACCATCAG GCCTATTGCT TTGAGAGCTG 
     3361 TGACCGCCAT TGCCCGCGCT TTGCCTGGGT TTCCTATACT GGCCACAGGT GGAATTGACT 
     3421 CAGCTGAAAG TGGACTTCAG TTTCTTCATA GTGGTGCTTC AGTTCTCCAG GTATGCAGTG 
     3481 CTATTCAGAA TCAGGACTTC ACTGTGATTG AAGATTACTG CACTGGCCTC AAAGCTCTGC 
     3541 TTTATCTGAA GAGTATTGAA GAGTTATCAG ACTGGGATGG GCAGAGTCCA CCCACTATGA 
     3601 GTCATCAGAA AGGGAAACCA GTTCCACACA TTGCTGAGCT CATGGGACAG AAACTTCCAA 
     3661 GCTTTGGACC GTACCTTGAA CGGCGCAAGA AAATCCTAGC AGCAAGTAAA ATCAGAGAGA 
     3721 ATGATCAAAA CAGAGCTTGC TCACCTCTCC AGAGAAAGCA CTTTAACTCC CAAAAGCCGA 
     3781 TTCCTGCCAT CAAGGATGTA ATTGGAAAAT CACTGCAATA CCTGGGGACG TTTGGTGAGC 
     3841 TGAACATCAT GGAGCAAGTT GTGGCCCTGA TCGATGAGGA AATGTGTATC AATTGCGGCA 
     3901 AATGTTACAT GACCTGTAAT GACTCTGGCT ACCAGGCTAT ACAGTTCGAT CCAGAAACTC 
     3961 ACCTGCCTAC TGTTAGCGAC ACATGTACAG GCTGCACTCT CTGCCTCAGC GTCTGCCCTA 
     4021 TTATGGACTG TATCAGGATG GTTTCCAGGG CAACACCTTA TGAACCAAAG AGAGGCCTAC 
     4081 CATTAGCCGT GAAGCCGGTG TGTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4141 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4201 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4261 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4321 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4381 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4441 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4501 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4561 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4621 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4681 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4741 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4801 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4861 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4921 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4981 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5041 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5101 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5161 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5221 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5281 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5341 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5401 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5461 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5521 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5581 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5641 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5701 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5761 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5821 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5881 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5941 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6001 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6061 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6121 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6181 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6241 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6301 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6361 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6421 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6481 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6541 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6601 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6661 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6721 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6781 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6841 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6901 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6961 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7021 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7081 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7141 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7201 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7261 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7321 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7381 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7441 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7501 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7561 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7621 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7681 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7741 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7801 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7861 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7921 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//