LOCUS       RR210305                7242 bp    DNA     circular  SYN 
DEFINITION  Itgb6 (Myc-DDK-tagged ORF) - Rat integrin, beta 6 (Itgb6), (10 ug)
ACCESSION   NM_001004263
VERSION     NM_001004263.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3399..3490 
                     /label="Myc-DDK"  
     misc_feature    3520..4105 
                     /label="PolyA signal"  
     misc_feature    3577..3596 
                     /label="Restriction Enzyme"  
     misc_feature    4381..5053 
                     /label="ColE1"  
     misc_feature    5483..6277 
                     /label="Kan/Neo"  
     misc_feature    6312..6614 
                     /label="SV40 ori"  
     misc_feature    6700..7177 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3381..3387 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3381 
                     /label="Itgb6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGATTGAG CTGGTCTGCC TGTTCCTGCT ACTTCTAGGA AGGAATGATC 
     1081 ACGTCCAAGG TGGCTGTGCC TGGAGTGGTG CAGAAACCTG CTCAGACTGC CTGCTCACAG 
     1141 GACCTCACTG CGCCTGGTGC TCCCAGGAGA ATTTCACCCA CCTGTCCGGA GCTGGCGAAA 
     1201 GGTGCGACAC CCCCGAGAAT CTTTTAGCCA AAGGATGTCA GTTACCCTTC ATCGAAAACC 
     1261 CTGTCTCCCA AGTAGAAATA CTTCAAAATA AGCCTCTCAG CGTAGGTCGA CAGAAAAACA 
     1321 GTTCTGACAT TGTTCAGATT GCTCCTCAAA GCTTGGTTCT TAAGTTGAGA CCAGGGGGTG 
     1381 AGCAGACTCT GCAAGTGCAG GTCCGCCAGA CGGAAGATTA CCCAGTAGAT CTGTATTACC 
     1441 TCATGGACCT CTCGGCCTCC ATGGATGATG ACCTCAACAC AATCAAAGAG CTTGGCTCCC 
     1501 GGCTGGCCAA AGAGATGTCT AAATTAACCA GCAACTTTAG ACTGGGCTTT GGCTCTTTTG 
     1561 TGGAAAAGCC CGTTTCTCCT TTTATGAAAA CAACACCAGA GGAAATCACC AACCCTTGCA 
     1621 GTAGTATCCC CTATTTCTGC TTACCTACAT TTGGATTCAA GCACATTTTG CCATTGACTG 
     1681 ATGATGCTGA GAGATTCAAT GAAATTGTGA GGAAACAGAA GATTTCCGCT AATATTGACA 
     1741 CGCCTGAAGG CGGATTCGAT GCCATCATGC AAGCTGCTGT GTGTAAGGAA AAGATTGGCT 
     1801 GGCGCAACGA CTCGCTCCAC CTCCTGGTTT TTGTGAGTGA TGCTGATTCT CATTTTGGAA 
     1861 TGGACAGCAA GCTGGCAGGC ATTGTGATTC CCAACGATGG GCTCTGTCAC TTGGACAACA 
     1921 GGAATGAATA CTCCATGTCA ACTGTCTTGG AATATCCAAC GATCGGCCAA CTCATTGATA 
     1981 AACTGGTACA AAACAACGTG CTACTGATCT TTGCGGTCAC CCAAGAACAA GTCCATCTCT 
     2041 ATGAGAATTA TGCGAAACTC ATCCCTGGAG CGACTGTGGG ACTGCTTCAG AAGGATTCTG 
     2101 GGAACATTCT TCAGCTGATC ATCTCCGCTT ACGAAGAACT GCGGTCTGAG GTGGAACTAG 
     2161 AAGTATTAGG AGACACAGAA GGACTCAACC TGTCTTTCAC AGCTCTCTGT AGCAATGGCA 
     2221 TCCTCTTCCC ACACCAAAAG AAGTGTTCCC ACATGAAAGT CGGAGATACA GCATCCTTCA 
     2281 ACGTGTCTGT GAGCATAACA AACTGTGAGA AAAGAAGCAG GAAACTTATC ATCAAGCCTG 
     2341 TGGGACTTGG GGACACCCTG GAAATACTCG TCAGCGCAGA ATGTGACTGC GACTGCCAGA 
     2401 GAGAAGTAGA AGCCAACAGT TCTAAGTGCC ACCACGGGAA TGGCTCCTTC CAGTGTGGGG 
     2461 TGTGTGCCTG CAACCCTGGC CACATGGGTC CTCGCTGCGA GTGCGGCGAG GACATGGTGA 
     2521 GCACGGATTC CTGCAAGGAG TCCCCAGGTC ACCCCTCCTG CAGCGGAAGG GGTGACTGCT 
     2581 ATTGTGGGCA GTGTGTCTGC CACTTGTCTC CCTATGGAAG CATCTATGGA CCTTACTGCC 
     2641 AGTGTGACAA CTTCTCCTGT CTGAGACACA AAGGCCTGCT CTGTGGAGAT AATGGTGACT 
     2701 GTGACTGTGG TGAATGCGTG TGCCGGGATG GCTGGACGGG CGAGTACTGC AACTGTACAA 
     2761 CCAGCAGGGA TGCGTGTGCA TCGGAAGATG GCGTGCTGTG CAGCGGGCGT GGGGACTGTG 
     2821 TCTGCGGCAA GTGTGTCTGC AGAAACCCTG GAGCCTCGGG ACCCACCTGT GAACGCTGCC 
     2881 CTACCTGTGG AGACCCCTGT AACTCGAGAC GGAGCTGCAT CGAGTGCTAC CTGTCTGCAG 
     2941 ATGGCCAGGC CCAAGAAGAG TGCGAGGACA AGTGCAAAGC CACCGGTGCC ACCATCAGTG 
     3001 AGGAAGAGTT TTCAAAGGAT ACTTCTGTCC CCTGCTCGCT ACAAGGAGAA AATGAATGTC 
     3061 TTATTACATT CCTAATAACT GCGGATAATG AAGGGAAAAC CATCATTCAC AACATCAGTG 
     3121 AAAAAGACTG CCCCAAACCT CCAAACATTC CCATGATCAT GTTGGGGGTG TCACTGGCTA 
     3181 TCCTGCTCAT CGGTGTCGTG CTACTGTGCA TTTGGAAGCT GCTGGTATCC TTTCACGACC 
     3241 GGAAAGAAGT TGCTAAATTT GAAGCAGAAC GATCAAAGGC CAAGTGGCAA ACGGGAACCA 
     3301 ATCCGCTGTA CCGAGGCTCC ACCAGCACTT TTAAGAACGT GACCTACAAA CACAGGGAGA 
     3361 AGCACAAAGT GGGCCTTTCT TCAGATGGGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3421 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3481 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3541 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3601 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3661 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3721 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3781 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3841 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3901 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3961 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4021 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4081 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4141 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4201 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4261 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4321 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4381 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4441 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4501 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4561 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4621 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4681 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4741 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4801 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4861 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4921 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4981 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5041 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5101 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5161 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5221 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5281 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5341 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5401 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5461 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5521 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5581 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5641 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5701 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5761 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5821 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5881 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5941 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6001 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6061 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6121 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6181 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6241 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6301 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6361 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6421 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6481 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6541 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6601 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6661 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6721 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6781 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6841 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6901 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6961 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7021 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7081 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7141 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7201 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//