LOCUS       RR210290                8217 bp    DNA     circular  SYN 
DEFINITION  Stard13 (Myc-DDK-tagged ORF) - Rat StAR-related lipid transfer (START) domain containing 13 (Stard13), (10 ug)
ACCESSION   NM_001109060
VERSION     NM_001109060.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4374..4465 
                     /label="Myc-DDK"  
     misc_feature    4495..5080 
                     /label="PolyA signal"  
     misc_feature    4552..4571 
                     /label="Restriction Enzyme"  
     misc_feature    5356..6028 
                     /label="ColE1"  
     misc_feature    6458..7252 
                     /label="Kan/Neo"  
     misc_feature    7287..7589 
                     /label="SV40 ori"  
     misc_feature    7675..8152 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4356..4362 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4356 
                     /label="Stard13"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCAGTCAG GTGCCCAGGA CGCCGGCTGC AGGCTGCTAC TGTCTAAATC 
     1081 CCTTGACACC TGAGAGCCAG GAGATGTACT TGCGATTTGA TCAGACTGCC AGACGCTCTC 
     1141 CCTACAGGAT GAGCCGGATC CTAGCGCGCC ATCACCTAGT GACTAAAATT CAGCAAGAAA 
     1201 TCGAGGCGAA GGAAGCATGT GACTGGCTGC GAGCTGCCGG GTTCCCACAG TACGCTCAGC 
     1261 TGTATGAAGA TTCGCAGTTT CCCATCAACA TTGCGGCTGT CAAAAAAGAC CATGATTTTC 
     1321 TTGAAAGGGA CCTTGTAGAA CCTCTTTGCA GACGACTTAA TACGTTGAAC AAGTGTGCCT 
     1381 CAATGAGACT TGATGTGAAC TTCCAAAGGA AAAAGGGCGA CGACTCAGAT GAGGAAGACC 
     1441 TGTGCATCAG CAACAAATGG ACTTTCCAGA GAACCAGCCG CAGGTGGTCC CGTGTGGATG 
     1501 ACCTGCATCC GCTCCTCCCC GGGGCAGACA GAAACGGGTC CCCAGGAGGC CCTAGGATGA 
     1561 GAAACACGGC CAGCAGTGAA AGCGTGCTCA CAGATCTGAG CGAGCCTGAG GTCTGCTCCA 
     1621 TCCACAGCGA AAGCAGCGGA GGCAGCGACG GCCGCGGCCA ATCGGGGCAT CATTTTGCTG 
     1681 ATGGCACGCC TGTGCTGGAG GGCACCCTAG TCGGTGGCAG CCTCCCGCAG TCTCCCCGAG 
     1741 ACAGTCTCAG CCAGTCTTTT CACCCCAAGA ATGAGAAACC CACCAGGACC AGGGCCAAGT 
     1801 CATTTCTGAG ACGCATGGAT ACCCTGAGAG TGAAGGGAGC ACTCGGGAGG CATAAGGGAC 
     1861 CAGGGCGAAC AGGAGGCCTC GTTATCAGCA GGCCCATGCT GCAGCAGGAG CCAGAGTCCT 
     1921 TTAAGACCAT GCAGCATGTC CAGATACCCA ACGGAGATCT GCAGACCTCA CCACCAGCTG 
     1981 CCTGCAAGAA AGGCCTTCCA GGCTCCAGTA AATCAAGCGG TGAGAACAGC CCCTTGGAGA 
     2041 GCAGCCGTGT GAGCACCCCA TGTATGAAGG AACGCAAGTG CCAACACGAG GCCAACAAGC 
     2101 GCGGTGGCAT GTACCTGGAG GACCTGGATG TGCTGGCAGG GACGACATTA CCAGACACAG 
     2161 GGGACCAAAA CCACATACAC GGGTTTCACT CGCAAGAGAA CCTGGTGGTC CATGTTCCCA 
     2221 AGGATCACAA ACCAGGAACA TTCCCCAAGG CACTGTCTAT AGAAAGCCTC TCGCCCACAG 
     2281 ACAGCAGCAA TGGGGTTAAC TGGAGGACTG GGAGTATCTC CCTGGGCAGG CAACAGGGCC 
     2341 CTGGCATAAG GGAACCTAGA CTCGTGTCAT CCTGCCACAG GGCCAGCAGA GTCAGTATCT 
     2401 ATGACAACGT GCCCAGCTCA CATCTGTATG CCAGCACTGG AGATCTGTTG GACTTGGAGA 
     2461 AAGACGGGCT CCTTCCACAG CTGGATGACA TTATACAGCA TGTCAATGGC ATACAAGAGG 
     2521 TAGTGGATGA CTGGTCAAGA AATATCTTGC CTGAACTGCA AAGTCACGAT GCAGTGGCAG 
     2581 GGGACCCTGG TCAACCCCCA TTTCAGTCTC CTCATCAGAT CACTTTAGAT TTTGAAGGCA 
     2641 ACTCTGTCTC GGAAGGTCAG ACAACACCTA GTGATGTGGA AAGGGACAGG ACTTCTCTGA 
     2701 ATGAATCGGA CACCACTGGG GTCAGAGAAA GAAGGGATTC TGGTGTGGGG GCCTCTCTGA 
     2761 CCAGGCCAAA CAGGCGCCTA AGGTGGAGTA GCTTCCAGCT CTCACACCAG CCTCAGCTGT 
     2821 CTCCGGCCAC GCCCCACATC AGCAGCCAGA CGGCCGCCCA GCTGAACCTG CTCCAGCGCT 
     2881 TTTCTCTGCT TCGCCTCACA GCCATCATGG AGAAGTACTC CATGTCCAAC AAGCACGGCT 
     2941 GGACCTGGTC CGTCCCAAAG TTCATGAAGA GAATCAAAGC TCCTGACTAC AGAGACAAGG 
     3001 CTGTCTTCGG TGTCCCGCTC ATAGTCCATG TCCAGAGAAC AGGACAGCCC CTGCCTCAGA 
     3061 GCATTCAACA AGCACTGAGG TATCTACGTA GCAACTGTCT GGATCAGGTG GGTCTTTTCC 
     3121 GAAAGTCGGG AGTGAAGTCT CGAATCCATG CCCTACGTCA GATGAATGAG AACTTCCCTG 
     3181 ACAACGTGAG CTATGAAGAC CAGTCTGCCT ATGACGTGGC AGATATGGTA AAGCAGTTCT 
     3241 TCCGGGACCT CCCTGAGCCC CTTTTCACCA ACAAGCTCAG TGAGACCTTC CTCCACATCT 
     3301 ATCAGTACGT CCCCAAAGAA CAGCGGCTGC AGGCCGTGCA GGCAGCCATC CTCCTGCTGG 
     3361 CGGATGAGAA CCGGGAGGCC CTGCAGACGC TCCTGTGCTT CCTGCATGAC GTAGTGAACT 
     3421 TGGTGGACGA GAATCAGATG ACGCCCATGA ATCTGGCCGT GTGCCTGGCC CCCTCCCTCT 
     3481 TTCATCTCAA CTTGCTGAAA AAAGAAAGTT CCCCAAGAGT CATCCAGAAG AAATACGCCA 
     3541 CTGGGAAGCC AGATCAGAAA GACCTCAATG AAAATCTAGC CGCAGCTCAG GGGCTCGCCC 
     3601 ACATGATCAC GGAATGCAAC CGACTCTTCG AGGTTCCACA CGAGATGGTG GCCCAGTCTC 
     3661 GTAACTCCTA CTTAGAGGCT GAGATCCACG CGCCCAGCCT AGAAGACTTG GGAACTCAGC 
     3721 TGGCAGAGAA TGGGGCAACT TTTCACACGT ACCTGGAGCA TCTTGTCCAA GGCCTCCAGA 
     3781 AAGAGGCTAA GGAGAAATTC AAGGGGTGGG TCACGTGTTC CAGCCCTGAC AACACGGACC 
     3841 TTGCTTTCAA AAAGGTGGGG GACGGGAACC CGCTGAAGCT GTGGAAGGCA TCCGTGGAGG 
     3901 TGGAGGCACC GCCCTCCGTG GTGTTGAACC GCGTGCTGAG AGAACGTCAC CTGTGGGATG 
     3961 AGGATTTCGT GCAGTGGAAG GTCGTGGAAA CGTTGGACAG ACAGACAGAA ATATACCAGT 
     4021 ATGTGCTAAA CAGCATGGTT CCACACCCCT CCAGAGACTT CGTGGTGCTC AGGACCTGGA 
     4081 AGACCGATTT GCCCAAAGGA ATGTGCACCC TGGTGTCCCT GTCTGTGGAG CATGAAGAAG 
     4141 CCCAGCTCAT GGGTGGTGTG CGGGCGGTGG TGATGGACTC TCAGTACCTG ATAGAGCCCT 
     4201 GTGGCTCCGG CAAGTCCAGG CTGACCCACA TCTGCAGGAT AGACCTGAAA GGCCACTCCC 
     4261 CAGAATGGTA CAGCAAAGGC TTCGGACACC TCTGTGCCGC AGAAGTTGCC AGAATTAGGA 
     4321 ACTCTTTCCA GCCTCTCGTT GCTGAGGGTC CAGAAACAAA AATCACGCGT ACGCGGCCGC 
     4381 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4441 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4501 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4561 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4621 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4681 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4741 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4801 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4861 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4921 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4981 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5041 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5101 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5161 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5221 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5281 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5341 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5401 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5461 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5521 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5581 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5641 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5701 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5761 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5821 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5881 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5941 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6001 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6061 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6121 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6181 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6241 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6301 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6361 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6421 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6481 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6541 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6601 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6661 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6721 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6781 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6841 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6901 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6961 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7021 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7081 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7141 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7201 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7261 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7321 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7381 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7441 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7501 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7561 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7621 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7681 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7741 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7801 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7861 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7921 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7981 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8041 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8101 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8161 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//