LOCUS       RR210260                7260 bp    DNA     circular  SYN 
DEFINITION  Smo (Myc-DDK-tagged ORF) - Rat smoothened homolog (Drosophila) (Smo), (10 ug)
ACCESSION   NM_012807
VERSION     NM_012807.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3417..3508 
                     /label="Myc-DDK"  
     misc_feature    3538..4123 
                     /label="PolyA signal"  
     misc_feature    3595..3614 
                     /label="Restriction Enzyme"  
     misc_feature    4399..5071 
                     /label="ColE1"  
     misc_feature    5501..6295 
                     /label="Kan/Neo"  
     misc_feature    6330..6632 
                     /label="SV40 ori"  
     misc_feature    6718..7195 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3399..3405 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3399 
                     /label="Smo"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCTGGC CGCCCCGTGC GTGGGCCCGA GCTGGCGCCC CGGAGGCTGC 
     1081 TGCAGTTGCT GCTGCTGGTA CTGCTTGGGG GCCGGGGCCG GGGGGCGGCC TTGAGCGGGA 
     1141 ACGTGACCGG GCCTGGGCCT CGCAGTGCCG GCGGGAGCGC GAGGAGGAAC GCGCCGGTGA 
     1201 CCAGCCCTCC GCCGCCGCTG CTGAGCCACT GCGGCCGGGC CGCCCACTGC GAGCCTTTGC 
     1261 GCTACAACGT GTGCCTGGGC TCCGCGCTGC CCTACGGAGC CACCACCACG CTGCTGGCTG 
     1321 GGGACTCGGA CTCGCAGGAG GAAGCGCACA GCAAGCTCGT GCTCTGGTCC GGCCTCCGGA 
     1381 ATGCTCCCCG ATGCTGGGCA GTGATCCAGC CCCTGCTGTG TGCTGTCTAC ATGCCCAAGT 
     1441 GTGAAAATGA CCGAGTGGAG TTGCCCAGCC GTACCCTCTG CCAGGCCACC CGAGGCCCCT 
     1501 GTGCCATTGT GGAGCGGGAA CGAGGGTGGC CTGACTTTCT GCGTTGCACG CCGGACCACT 
     1561 TCCCTGAAGG CTGTCCAAAC GAGGTACAAA ACATCAAGTT CAACAGTTCA GGCCAATGTG 
     1621 AAGCACCCTT GGTGAGGACA GACAACCCCA AGAGCTGGTA CGAGGACGTG GAGGGCTGTG 
     1681 GGATCCAGTG CCAGAACCCG CTGTTCACCG AGGCTGAGCA CCAGGACATG CACAGTTACA 
     1741 TCGCAGCCTT CGGGGCGGTC ACCGGCCTCT GTACACTCTT CACCCTGGCC ACCTTTGTGG 
     1801 CTGACTGGCG GAACTCCAAT CGCTACCCTG CGGTTATTCT CTTCTATGTC AATGCGTGTT 
     1861 TCTTTGTGGG CAGCATTGGC TGGCTGGCCC AGTTCATGGA TGGTGCCCGC CGGGAGATTG 
     1921 TTTGCCGAGC AGATGGCACC ATGAGATTTG GGGAGCCCAC CTCCAGCGAG ACCCTATCCT 
     1981 GTGTCATCAT CTTTGTCATC GTGTACTATG CCTTGATGGC TGGAGTAGTG TGGTTCGTGG 
     2041 TCCTCACCTA TGCCTGGCAC ACCTCCTTCA AAGCCCTGGG CACCACTTAC CAGCCTCTCT 
     2101 CGGGCAAGAC ATCCTATTTC CACCTGCTCA CGTGGTCACT CCCCTTCGTC CTCACTGTGG 
     2161 CAATCCTTGC TGTGGCTCAG GTAGATGGGG ACTCCGTGAG TGGCATCTGC TTTGTAGGCT 
     2221 ACAAGAACTA TCGGTACCGT GCTGGCTTTG TACTTGCCCC AATTGGCCTG GTGCTTATTG 
     2281 TGGGAGGCTA CTTCCTCATC CGAGGGGTCA TGACTCTGTT CTCCATCAAG AGCAACCACC 
     2341 CTGGGCTTCT GAGTGAGAAG GCAGCCAGCA AGATCAATGA GACCATGCTG CGCCTGGGCA 
     2401 TTTTTGGCTT CCTCGCCTTT GGCTTCGTGC TCATCACCTT CAGCTGCCAC TTCTATGACT 
     2461 TCTTCAACCA GGCTGAGTGG GAGCGTAGCT TCCGGGACTA TGTGCTATGC CAAGCCAATG 
     2521 TGACCATTGG GCTGCCTACC AAGAAGCCCA TTCCTGATTG TGAGATCAAG AATCGGCCCA 
     2581 GCCTCCTGGT GGAGAAGATC AATCTGTTTG CCATGTTTGG CACTGGCATT GCCATGAGCA 
     2641 CCTGGGTCTG GACCAAGGCC ACCCTGCTCA TCTGGAGGCG CACCTGGTGC AGGTTGACTG 
     2701 GGCACAGTGA TGATGAACCC AAGAGAATCA AGAAAAGCAA GATGATTGCC AAGGCCTTCT 
     2761 CTAAGCGGCG TGAACTGCTG CAGAACCCGG GCCAGGAGCT CTCCTTCAGC ATGCACACTG 
     2821 TCTCCCATGA TGGACCTGTT GCCGGTTTGG CTTTTGAACT CAATGAACCC TCAGCTGATG 
     2881 TCTCCTCTGC CTGGGCCCAG CACGTCACCA AGATGGTGGC TCGAAGAGGA GCCATATTAC 
     2941 CCCAGGATGT GTCTGTCACC CCTGTGGCAA CTCCAGTGCC ACCAGAAGAA CAAGCCAACC 
     3001 TGTGGCTGGT TGAGGCAGAG ATCTCCCCAG AGTTAGAGAA GCGTTTAGGC CGGAAGAAGA 
     3061 AGCGGAGGAA GAGGAAGAAG GAGGTGTGCC CCTTGGGGCC AGCCCCTGAA CTTCACCACT 
     3121 CTGCCCCTGT TCCTGCCACC AGTGCAGTTC CTCGGCTGCC TCAGCTGCCT CGGCAGAAGT 
     3181 GCCTAGTAGC TGCAAATGCC TGGGGAACAG GAGAGCCCTG CCGACAGGGA GCCTGGACTG 
     3241 TAGTCTCCAA CCCCTTCTGC CCAGAGCCTA GTCCCCATCA AGATCCATTT CTCCCTGGTG 
     3301 CCTCAGCCCC CAGGGTCTGG GCTCAGGGCC GCCTCCAGGG GCTGGGATCC ATTCATTCCC 
     3361 GCACTAACCT AATGGAGGCT GAGCTCTTGG ATGCAGACTC GGACTTCACG CGTACGCGGC 
     3421 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3481 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3541 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3601 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3661 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3721 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3781 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3841 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3901 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3961 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4021 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4081 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4141 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4201 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4261 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4321 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4381 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4441 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4501 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4561 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4621 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4681 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4741 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4801 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4861 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4921 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4981 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5041 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5101 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5161 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5221 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5281 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5341 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5401 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5461 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5521 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5581 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5641 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5701 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5761 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5821 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5881 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5941 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6001 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6061 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6121 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6181 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6241 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6301 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6361 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6421 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6481 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6541 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6601 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6661 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6721 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6781 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6841 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6901 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6961 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7021 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7081 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7141 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7201 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//