LOCUS       RR210250                8409 bp    DNA     circular  SYN 
DEFINITION  Plcb4 (Myc-DDK-tagged ORF) - Rat phospholipase C, beta 4 (Plcb4), (10 ug)
ACCESSION   NM_024353
VERSION     NM_024353.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4566..4657 
                     /label="Myc-DDK"  
     misc_feature    4687..5272 
                     /label="PolyA signal"  
     misc_feature    4744..4763 
                     /label="Restriction Enzyme"  
     misc_feature    5548..6220 
                     /label="ColE1"  
     misc_feature    6650..7444 
                     /label="Kan/Neo"  
     misc_feature    7479..7781 
                     /label="SV40 ori"  
     misc_feature    7867..8344 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4548..4554 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4548 
                     /label="Plcb4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAAACCT TACGAATTTA ACTGGCAGAA GGAGGTACCC TCTTTCTTGC 
     1081 AAGAAGGAGC AGTTTTTGAC AGATATGAAG AGGAATCTTT CGTGTTTGAG CCCAACTGCC 
     1141 TCTTCAAAGT GGATGAATTC GGCTTCTTCC TGACCTGGAA GAGTGAAGGC AAGGAAGGAC 
     1201 AAGTGCTAGA ATGTTCCCTC ATCAACAGCA TTCGGCTGGC AGCCATACCA AAGGATCCCA 
     1261 AAATCCTGGC TGCTCTCGAG TCTGTTGGAA AATCTGAAAA TGATCTGGAA GGGCGGATAT 
     1321 TGTGTGTCTG CAGCGGCACG GATCTGGTGA ATATTGGCTT CACTTACATG GTGGCTGAAA 
     1381 ACCCAGAAAT AACTAAGCAA TGGGTAGAAG GCCTGAGATC AATCATTCAC AACTTCAGGG 
     1441 CAAACAACGT CAGTCCGATG ACATGCCTCA AGAAACACTG GATGAAACTG GCCTTTCTGA 
     1501 CCAACACAAG TGGCAAAATC CCAGTGAGGA GTATTACTAG AACCTTCGCA TCAGGGAAAA 
     1561 CAGAAAAGGT GATCTTTCAA GCACTCAAGG AATTAGGTCT CCCCAGTGGA AAGAATGATG 
     1621 AAATTGAACC TGCTGCATTT ACCTATGAAA AGTTCTATGA ACTGACACAG AAGATTTGTC 
     1681 CTCGGACAGA TATAGAAGAT CTTTTTAAAA AAATCAATGG AGACAAAACT GATTATTTAA 
     1741 CGGTAGACCA ATTAGTGAGC TTTCTAAACG AACATCAGCG AGATCCTCGG CTGAATGAAA 
     1801 TTTTATTCCC GTTTTATGAT GCTAAAAGAG CAATGCAGAT CATTGAGATG TATGAACCTG 
     1861 ATGAAGAGTT GAAGAAAAAA GGCCTCATAT CCAGTGATGG ATTCTGCAGA TATCTCATGT 
     1921 CAGATGAAAA TGCCCCCGTC TTCCTAGATC GCTTAGAACT TTACCAGGAG ATGGACCACC 
     1981 CCCTGGCCCA TTACTTCATC AGTTCCTCTC ACAACACCTA TCTCACCGGC CGGCAGTTTG 
     2041 GCGGAAAGTC TTCAGTGGAA ATGTACCGAC AAGTCCTCCT GGCTGGTTGC AGGTGTGTGG 
     2101 AACTTGACTG TTGGGATGGA AAAGGTGAAG ATCAGGAACC AATAATAACT CACGGAAAAG 
     2161 CAATGTGTAC AGACATCCTT TTTAAGGATG TAATCCAGGC CATCAAGGAA ACAGCGTTTG 
     2221 TCACATCAGA ATATCCTGTA ATTCTCTCCT TTGAAAACCA CTGCAGCAAA TATCAACAGT 
     2281 ACCAGATGTC CAAGTATTGT GAAGATCTAT TTGGGGATCT TCTGTTGAAA CAAGCACTTG 
     2341 AGTCACATCC ACTTGAACCA GGGAGGCTCT TGCCATCTCC TAATGACCTC AAAAGAAAAA 
     2401 TACTCATCAA AAATAAAAGG CTGAAGCCTG AAGTTGAAAA AAAACAGCTG GAGGCTTTGA 
     2461 AAAGCATGAT GGAGGCTGGA GAATCAGCTG CCCCGGCCAG CATCTTGGAA GATGACAATG 
     2521 AGGAGGAAAT AGAAAGCGCA GCTGATCAAG AGGAAGAAGC CCACCCTGAA TACAAGTTTG 
     2581 GAAATGAACT TTCTGCCGAT GACTTCAGTC ACAAGGAAGC GGTTGCAAAC AGCGTCAAGA 
     2641 AGGGCCTGGT CACCGTAGAA GATGAGCAGG CATGGATGGC ATCTTATAAA TACGTAGGTG 
     2701 CTACCACGAA TATCCATCCG TACTTGTCCA CGATGATCAA CTATGCACAG CCTGTGAAGT 
     2761 TTCAAGGTTT CCATGTGGCT GAAGAGCGCA ACATTCATTA TAACATGTCT TCTTTTAACG 
     2821 AGTCGGTTGG CCTTGGCTAC TTGAAGACGC ACGCGATTGA GTTTGTAAAT TACAATAAGC 
     2881 GACAAATGAG CCGCATTTAC CCCAAGGGAG GGCGAGTTGA TTCCAGTAAT TACATGCCTC 
     2941 AGATTTTCTG GAACGCTGGT TGCCAGATGG TTTCACTGAA CTATCAAACC CCAGATTTAG 
     3001 CGATGCAATT GAATCAAGGA AAATTTGAGT ATAACGGATC CTGCGGATAC CTCCTCAAGC 
     3061 CAGACTTCAT GAGACGGCCT GATCGGACAT TTGATCCCTT CTCTGAAACC CCTGTGGACG 
     3121 GGGTTATTGC AGCCACATGC TCAGTGCAGG TTATATCAGG GCAGTTCCTG TCAGATAAGA 
     3181 AGATCGGCAC ATACGTGGAA GTCGATATGT ACGGGCTGCC CACCGACACC ATACGGAAGG 
     3241 AGTTCCGAAC CCGCATGGTT ATGAACAATG GACTCAACCC AGTTTACAAT GAAGAATCGT 
     3301 TCGTGTTTCG GAAGGTGATC CTGCCTGACC TGGCTGTCCT GAGAATCGCT GTGTACGACG 
     3361 ACAACAACAA GCTCATTGGC CAGAGGATCC TTCCTCTGGA TGGCCTCCAG GCAGGTTACC 
     3421 GACACATCTC CCTGAGAAAT GAGGGAAACA AACCGTTATC ACTGCCAACA ATTTTCTGCA 
     3481 ATATCGTTCT TAAAACATAT GTGCCTGATG GATTGGGAGA TATTGTGGAT GCTCTATCCG 
     3541 ATCCAAAGAA ATTCCTTTCA ATCACAGAGA AGAGAGCAGA CCAATTGAGA GCAATGGGCA 
     3601 TTGAAACTAG TGACATAGCA GATGTGCCCA GTGACACTTC CAAAAATGAC AAGAAAGGCA 
     3661 AGGCCAACCC AGCCAAAGCG AATGTAACCC CTCAGAGCAG CTCTGAGCTC AGACCAACCA 
     3721 CCACAGCTGC CCTGGGATCT GGCCAGGAAG CCAAGAAAGG TATTGAACTT ATCCCTCAAG 
     3781 TGAGGATAGA AGATTTAAAG CAAATGAAGG CTTACTTGAA GCATTTAAAG AAACAACAAA 
     3841 AGGAGTTAAA CTCTTTAAAG AAGAAACACG CAAAGGAGCA CAGTACCATG CAGAAGTTAC 
     3901 ACTGCACACA AGTGGACAAA ATCGTGGCCC AGTATGACAA GGAGAAGTCG ACTCATGAGA 
     3961 AAATCCTAGA GAAGGCCATG AAGAAGAAGG GGGGAAGTAA TTGCCTTGAA ATAAAAAAAG 
     4021 AAACAGAAAT TAAGATTCAG ACCCTGACAT CGGATCACAA ATCTAAGGTC AAGGAGATTG 
     4081 TGGCGCAGCA CACAAAGGAG TGGTCAGAAA TGATCAACAC GCACAGCGCC GAGGAGCAGG 
     4141 AAATCCGGGA CCTGCACCTG AGCCAGCAGT GTGAGCTGCT GAGGAAGCTG CTCATCAACG 
     4201 CTCATGAGCA GCAGACCCAG CAGCTGAAGC TCTCCCATGA CAGGGAAAGC AAAGAGATGC 
     4261 GAGCCCATCA GGCTAAGATT TCTATGGAAA ATAGCAAAGC CATCAGTCAA GATAAATCTA 
     4321 TCAAGAATAA GGCAGAACGG GAGAGGCGAG TCAGGGAGTT GAACAGCAGC AACACTAAGA 
     4381 AGTTTCTAGA AGAAAGAAAG AGACTGGCAA TGAAGCAGTC GAAAGAAATG GATCAGCTGA 
     4441 AAAAGGTCCA GCTGGAGCAC CTAGAATTCC TAGAGAAACA GAATGAGCAG GCGAAGGAGA 
     4501 TGCAGCAGAT GGTGAAATTG GAAGCCGAGA TGGACCGCAG ACCAGCAACA GTAGTAACGC 
     4561 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4621 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4681 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4741 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4801 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4861 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4921 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4981 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5041 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5101 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5161 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5221 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5281 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5341 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5401 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5461 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5521 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5581 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5641 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5701 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5761 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5821 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5881 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5941 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6001 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6061 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6121 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6181 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6241 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6301 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6361 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6421 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6481 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6541 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6601 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6661 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6721 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6781 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6841 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6901 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6961 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7021 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7081 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7141 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7201 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7261 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7321 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7381 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7441 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7501 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7561 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7621 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7681 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7861 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7921 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7981 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8041 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8101 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8161 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8221 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8281 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8341 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8401 GCGAATTTT
//