LOCUS       RR210061                8841 bp    DNA     circular  SYN 
DEFINITION  Igsf1 (Myc-DDK-tagged ORF) - Rat immunoglobulin superfamily, member 1 (Igsf1), (10 ug)
ACCESSION   NM_175763
VERSION     NM_175763.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4998..5089 
                     /label="Myc-DDK"  
     misc_feature    5119..5704 
                     /label="PolyA signal"  
     misc_feature    5176..5195 
                     /label="Restriction Enzyme"  
     misc_feature    5980..6652 
                     /label="ColE1"  
     misc_feature    7082..7876 
                     /label="Kan/Neo"  
     misc_feature    7911..8213 
                     /label="SV40 ori"  
     misc_feature    8299..8776 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4980..4986 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4980 
                     /label="Igsf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGCTTCGG ACCTTCACTC TTTTGCTCCT TTGCATTTGG CTCAATCGGG 
     1081 GTATGACTTC AATGGCAGCA GTGGAGTCTC AACCAGAGCT ATGGATAGAG TCCAACTACC 
     1141 CCCAGGCCCC TTGGGAGAAC ATCACACTTT GGTGCAAAAG CCCCTCTCGG GTATCCAGCA 
     1201 AGTTCCTGCT GCTGAAGGAT AACACACAGA TGACCTGGAT TCATCCTCCT TACAAGACCT 
     1261 TCCAAGTTTC ATTCTTCATA GGTGCCCTTA CTGAGTCCAA TACAGGTCTT TATCGATGCT 
     1321 GCTACTGGAA TGAGAAAGGC TGGTCGAAGC CCAGTAAAAT TCTAGAGCTA GAAGCACCAG 
     1381 GCCAACTCCC TAAACCTATC TTCTGGATCC AGGCAGAGAC CCCCCCTTTT CCTGGATGCA 
     1441 ATGTTAATAT TCTCTGCCAT GGATGGCTTC AGGATTTGGT ATTTATGCTG TTTAAAGAGG 
     1501 GATACACAGA GCCCATAGAT TACCAAGTCC CAACTGGGAC AATGGCCATC TTCTCCATTG 
     1561 ACAACTTGGC ACCTGAGAAT GAAGGCATTT ACATCTGCCG CACCCATATC CAGATGCTCC 
     1621 CCACTCTGTG GTCAGAGCCC AGCAACCCCC TAAAGTTGGT GGTAGCAGGT CTCTACCCCA 
     1681 AACCAACTCT GACAGCCCAT CCTGGGCCCA TCCTGGCACC AGGAGAAAGC CTGAGTCTCA 
     1741 GGTGCCAAGG GCCAATATAT GGAATGACGT TTGCTTTAAT GAGGTTGGAA GACTTGAAGA 
     1801 AACCATATTA CTACAAGAAG CCAATAAAAA ATGAGGCATA TTTCTACTTC CAGGCTCTGA 
     1861 AGACACAGGA TACAGGGCAC TACCTCTGTT TTTACTATGA TGGGTCATAC AGGGGCTCAC 
     1921 TGCTTAGTGA TATCCTGAAA ATCTGGGTGA CTGACACTTT TCCCAAGACC TGGCTACTTG 
     1981 TTCAGCCCAG CCCTGTGATT CAAATGGGTC AAAACGTGAG CCTGAGATGT GGAGGACTGA 
     2041 TGGATGGAGT GGGTCTTGCA CTCCATAAGA AGGGAGAAGA AAAGCCCCTT CAGTTTCTGG 
     2101 ATGCCACCAG CAACACTGGC AACAATTCAT TCTTCCTAAA GAATGTGACC TACAGGGATG 
     2161 CTGGCATCTA TAGCTGCCAC TATTATCTTA CCTGGAAGAC TTCCATTAAG ATGGCGACAT 
     2221 ACAACACTGT GGAGCTGATT GTTGTAGCTT GGCCCAGTTC CGTGTTCAAA GTAGGAAAGA 
     2281 CCATCACCCT TCAGTGCCGA GTATCTCATC CAGTTCTTGA ATTTTCTTTG GAATGGGAAG 
     2341 AAACAACAAC ATTCCAAAAA TTCTCAGTAG ACGGAGACTT CATCATCACT AACATTGAAG 
     2401 GGCAAGGCAC GGGTACCTAC AGTTGCAGCT ATCGTATTGA GTCACACGCT AACATCTGGT 
     2461 CACATCGCAG TGAGCCTCTC AAGTTGGTGG GGCCAGCAGT CACAGGCTTC CTCCCCTGGA 
     2521 ATTCCATTCT GAATGAAGCT ATCAGGGTGT CCTTAACGGT GCAGTTTCTT TCCTTGCTGT 
     2581 TGCTAGTGCT GTGGCTACAA TGGAAGTGTC GGAGACTGAG ACTCAGAGAA GCCTGGTTGT 
     2641 TGGGAACAGC TCAAGGGGTT GCCATGCTCG TCATACTCAT AGCTCTTCTT TGCTGTGGGC 
     2701 TGTGCAATGG GGCATTGACA GAGGAGATTG AAATCATCAT GCCAACCCCT AAACCTGAAC 
     2761 TGTGGGCAGA GACGAACTTC CCTCAGGCCC CGTGGAAGAA CGTAACCCTC TGGTGCAGAA 
     2821 GCCCTTCTGG CTCAACTAAG GAGTTTGTGC TACTAAAGGA CGGGACTGGG TGGATTGCAA 
     2881 CTCGCCCGGC CTCAGAGCAG GTCCGGGCTG CCTTCCCCCT TGGCGCCCTG ACCCAAAGCC 
     2941 ACACCGGGAG TTACCATTGC CATTCATGGG AGGAGATGGC TGTGTCGGAG CCTAGTGAAG 
     3001 CACTTGAACT AGTGGGGACA GACATCCTTC CCAAACCTGT CATTTCGGCT TCCCTTCCAA 
     3061 TCCGGGGCCA GGAACTGCAG ATCCGATGTA AAGGATGGCT GGAAGGCTTG GGGTTTGCTC 
     3121 TGTATAAGAT GGGAGAGCAG GAACCTGTCC AGCAACTCGG TGCTGTTGGG AGAGAAGCCT 
     3181 TCTTTACAAT TCAAAGAATG GAGGATAAAG AGGAGGGCAA TTACAGCTGC CGCACACATA 
     3241 CTGAAAAGCA GCCCTTCAAG TGGTCTGAGC CCAGTGCTCC CCTGGAGCTT GTCATAAAAG 
     3301 AACTGTACCC CAAGCCTTTC TTCAAGACAT GGGCTAGCCC CGTGGTCACT CCTGGTTCTA 
     3361 GAGTGACTTT CAATTGCTCC ACCTCCCGGG AACACATGAG TTTTATTCTT TACAAGGATG 
     3421 GCAATGAAAT TGCATCCAGT GACCCAGTCT GGGGAAACCC AGGAACCAGC ACAGCACACT 
     3481 TTCTGATCAT TTCAGTGGGC ATTGGTGATG GAGGAAACTA TAGCTGCCGT TATTATGACT 
     3541 TTTCCATCTG GTCTGAGCCC AGCGACCCCG TGGAGCTTAT TGTGACAGAA TTCTACCCCA 
     3601 AACCCACTCT CCTGGCACAG CCAGGCCCTG TGGTGCTTCC TGGGAAGAAT GTGACCCTGC 
     3661 GCTGTCAAGG AATTTTCCAG GGCATGAGGT TTGCCCTCTT ACAGGAGGGT GCTCATGCTC 
     3721 CCTTACAGTT TCAGAGTGCC TCGGGGACCT CCGTTGACTT CCTCCTCCAC ACTGTTGGAG 
     3781 CAGAGGACTT TGGGAACTAC AGCTGTGTCT ACTATGAGAC AACCATGTCC AACAGGGGCT 
     3841 CATATCTTAG TACCCCCCTT ATGATCTGGG TGACTGACAC ATTCCCCAGA CCATGGTTGT 
     3901 CTGCTGAGCC TAGTTCTGTG GTCACCATGG GGCAGAATGT TACTCTATGG TGCCAAGGAC 
     3961 CAGTCCATGG AGTTGGGTAC ATTCTGCACA AAGAAGGAGA AGCCACTTCA ATGCAGCTTT 
     4021 GGGATTCCAC TAGCAATGAA GGGGCATTCC CTATCATCAA TATATCTGGT GCTAGCATAG 
     4081 GACGTTATAG CTGCTGTTAC CACCCTGACT GGATGAGCCC TATCAAGATA CAGCCTAGCA 
     4141 ACACTCTGGA ACTCATAGTC ACTGGTTTAC TCCCTAAGCC CAGCCTGTTA GTCCAGCCTG 
     4201 GTCCCATGGT AGTCCCCGGA GAAAACATGA CTTTTCAATG TCAAGGAGAA CTGCCAGATT 
     4261 CGACATTTGT CCTGTTGAAG GAGGGGACAC AACAGCCCAT AGAACAACAG AGGCCAAGTG 
     4321 GGTACAGAGC TGACTTCTGG ATGCCAGTGG TGAGGGATCA AGATTCTGGT GTTTACAGCT 
     4381 GTGTTTATTA TCTGGATTCT GCTCCCCTTG TGGCTTCTAA TCACAGCAAC TCTCTAGAGA 
     4441 TCTGGGTGAC CGATAAGCCC CCTAAACCCT CTCTGTCAGC GTGGCCCAGC ACTGTGTTCA 
     4501 AACTAGGAAA GGATATCACC CTTCAGTGCC GAGGACCCCT GCCAGGAGTT GAATTCGTGC 
     4561 TAGAACACGA TGGAGAAGAG GCCCCCCAAC AGTTCTCAGA GGATGGAGAT TTTGTTATCG 
     4621 GCAACATGGA AGGGAAAGGC ATTGGAAACT ACAGTTGCAG CTATCGGCTG CAGGCTTACC 
     4681 CTGACATCTG GTCAGAGCCC AGCGACTCCC TGGAGCTGGT TGGAGCCGCA GGGCCTGTTG 
     4741 CTCAAGAGTG CACTGTGGGT AACATTGTCC GAAGTACCCT GATTGTGGTG GTTGTGGTAG 
     4801 CCTTGGGGAT AGTGCTAGCC ATAGAGTGGA AGAAGTGGCC TCGACTTCGG ACTAGGGGCT 
     4861 CTGAGACAGA TGGGAGAGAC CAGACCATAG TCCTTGAAGA GTGTAACCAA GATGGAGAAC 
     4921 CCGGCACTGC CACCAATTCT CCTTCATCTG CTTTGCAGGG TGTCTCAGTG GAACAAACAG 
     4981 TCCCAATAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5041 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5101 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5161 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5221 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5281 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5341 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5401 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5461 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5521 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5581 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5641 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5701 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5761 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5821 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5881 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5941 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6001 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6061 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6121 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6181 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6241 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6301 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6361 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6421 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6481 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6541 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6601 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6661 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6721 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6781 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6841 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6901 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6961 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7021 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7081 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7141 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7201 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7261 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7321 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7381 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7441 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7501 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7561 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7621 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7681 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7741 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7801 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7861 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7921 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7981 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8041 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8101 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8161 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8221 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8281 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8341 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8401 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8461 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8521 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8581 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8641 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8701 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8761 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8821 CAAAAATTTA ACGCGAATTT T
//