LOCUS       RR210046                6732 bp    DNA     circular  SYN 
DEFINITION  F2 (Myc-DDK-tagged ORF) - Rat coagulation factor II (thrombin) (F2), (10 ug)
ACCESSION   NM_022924
VERSION     NM_022924.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2889..2980 
                     /label="Myc-DDK"  
     misc_feature    3010..3595 
                     /label="PolyA signal"  
     misc_feature    3067..3086 
                     /label="Restriction Enzyme"  
     misc_feature    3871..4543 
                     /label="ColE1"  
     misc_feature    4973..5767 
                     /label="Kan/Neo"  
     misc_feature    5802..6104 
                     /label="SV40 ori"  
     misc_feature    6190..6667 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2871..2877 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2871 
                     /label="F2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGCACGTC CGCGGCCTGG GCCTACCTGG CTGCCTGGCT CTGGCTGCCC 
     1081 TGGCTAGCCT GGTACACAGT CAGCATGTGT TCCTGGCCCC TCAGCAAGCA CTGTCGCTGC 
     1141 TCCAGCGGGT CCGCAGAGCC AACAGCGGCT TCCTGGAGGA GCTCCGAAAG GGCAACCTGG 
     1201 AGCGTGAGTG CGTGGAAGAG CAGTGCAGCT ATGAGGAGGC TTTTGAGGCC CTGGAGTCTC 
     1261 CCCAAGACAC GGATGTGTTC TGGGCCAAGT ACACAGTGTG TGATTCAGTG AGGAAACCTC 
     1321 GAGAGACCTT CATGGACTGT CTAGAAGGTC GCTGTGCTAT GGATCTGGGT CTGAACTACC 
     1381 ATGGGAATGT GAGTGTCACC CATACCGGCA TCGAGTGTCA GCTGTGGCGA AGCCGCTACC 
     1441 CACACAGGCC TGACATCAAC TCCACCACCC ATCCTGGGGC TGACCTGAAG GAGAACTTCT 
     1501 GCCGAAATCC AGACAGCAGC ACCTCAGGGC CCTGGTGCTA CACCACAGAC CCTACTGTGC 
     1561 GCAGAGAGGA ATGCAGTATC CCTGTCTGTG GTCAGGAGGG CCGTACCACT GTGAAAATGA 
     1621 CTCCTCGCTC AAGAGGTTCC AAGGAGAATC TGTCGCCTCC ATTGGGAGAG TGCCTCCTCG 
     1681 AGCGTGGCCG GCTGTACCAG GGGAACTTAG CTGTGACAAC ACTTGGGTCC CCCTGCCTGG 
     1741 CCTGGGACAG TTTGCCAACC AAGACCCTGA GCAAGTACCA GAACTTCGAC CCTGAGGTGA 
     1801 AACTCGTGCA AAACTTCTGC CGCAATCCAG ACCGGGATGA GGAGGGCGCG TGGTGCTTTG 
     1861 TTGCGCAGCA GCCTGGTTTT GAATACTGCA GCCTCAACTA CTGTGATGAA GCGGTGGGAG 
     1921 AGGAGAACCA TGACGGGGAC GAGTCCATCG CAGGACGCAC CACCGACGCG GAGTTCCACA 
     1981 CCTTCTTCGA TGAGAGGACT TTCGGCCTTG GGGAGGCAGA CTGTGGCCTG CGGCCTTTGT 
     2041 TCGAGAAGAA GTCCTTGACA GACAAAACGG AAAAGGAGCT TCTTGATTCT TACATAGATG 
     2101 GGCGCATCGT GGAGGGCTGG GACGCTGAGA AGGGTATCGC CCCATGGCAG GTGATGCTTT 
     2161 TTCGGAAGAG TCCCCAAGAG CTGCTGTGTG GAGCCAGCCT TATCAGTGAT CGATGGGTCC 
     2221 TCACTGCTGC CCACTGCATT CTGTACCCAC CCTGGGACAA GAACTTCACT GAGAATGACC 
     2281 TCCTGGTGCG CATTGGCAAG CACTCCAGAA CCAGATATGA GCGGAATGTT GAAAAGATCT 
     2341 CCATGCTGGA AAAGATCTAC ATCCACCCCA GATATAACTG GCGGGAAAAC CTAGACCGTG 
     2401 ACATTGCTCT GCTCAAGCTA AAGAAGCCAG TGCCCTTCAG TGACTACATT CACCCCGTGT 
     2461 GCTTGCCAGA CAAGCAGACA GTAACCAGTT TGCTCCAGGC TGGTTATAAA GGGCGGGTGA 
     2521 CAGGCTGGGG CAACCTTCGG GAGACGTGGA CAACTAACAT CAACGAGATA CAGCCCAGCG 
     2581 TCCTGCAGGT GGTGAACCTG CCCATTGTAG AGCGACCAGT GTGCAAGGCC TCTACCCGGA 
     2641 TACGCATTAC TGACAACATG TTCTGTGCTG GCTTCAAGGT GAATGACACC AAGCGAGGAG 
     2701 ATGCTTGTGA AGGTGACAGT GGGGGACCTT TTGTCATGAA GAGCCCCTAC AACCACCGCT 
     2761 GGTACCAAAT GGGTATTGTC TCCTGGGGTG AAGGCTGTGA CCGGAATGGG AAATATGGCT 
     2821 TCTACACGCA CGTGTTCCGT CTGAAAAGGT GGATGCAGAA AGTCATTGAT CAGCATAGAA 
     2881 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     2941 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3001 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3061 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3121 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3181 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3241 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3301 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3361 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3421 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     3481 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     3541 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     3601 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     3661 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     3721 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     3781 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     3841 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     3901 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     3961 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4021 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4081 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4141 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4201 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4261 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4321 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4381 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4441 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     4501 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     4561 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     4621 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     4681 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     4741 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     4801 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     4861 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     4921 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     4981 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5041 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5101 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5161 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5221 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5281 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5341 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5401 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     5461 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     5521 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     5581 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     5641 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     5701 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     5761 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     5821 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     5881 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     5941 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6001 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6061 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6121 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6181 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6241 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6301 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6361 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6421 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     6481 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     6541 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     6601 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     6661 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     6721 AACGCGAATT TT
//