LOCUS       RR210033                9309 bp    DNA     circular  SYN 
DEFINITION  Cftr (Myc-DDK-tagged ORF) - Rat cystic fibrosis transmembrane conductance regulator homolog (Cftr), (10 ug)
ACCESSION   NM_031506
VERSION     NM_031506.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5466..5557 
                     /label="Myc-DDK"  
     misc_feature    5587..6172 
                     /label="PolyA signal"  
     misc_feature    5644..5663 
                     /label="Restriction Enzyme"  
     misc_feature    6448..7120 
                     /label="ColE1"  
     misc_feature    7550..8344 
                     /label="Kan/Neo"  
     misc_feature    8379..8681 
                     /label="SV40 ori"  
     misc_feature    8767..9244 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5448..5454 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5448 
                     /label="Cftr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAAGTCG CCTCTGGAGA AAGCCAGCTT TATCTCCAAA CTCTTCTTCA 
     1081 GCTGGACCAC ACCAATTTTG AGAAAAGGGT ACAGACACCA CTTGGAGCTG TCAGACATAT 
     1141 ACCAAGCCCC TTCTTCTGAT TCAGCTGACC ACCTGTCTGA AAAGCTAGAA AGAGAATGGG 
     1201 ACAGGGAACA AGCTTCCAAA AAGAAGCCCC AGCTTATCCA CGCCCTCCGG CGATGCTTTG 
     1261 TCTGGAGATT CGTCTTCTAC GGAGTCTTGT TATACCTCGG GGAAGTCACC AAGGCTGTCC 
     1321 AGCCTGTCTT GCTAGGAAGA ATCATAGCAT CCTATGATCC AGACAACACG GAGGAGCGTT 
     1381 CCATTGCCAT TTACCTAGGC ATAGGCTTAT GCCTCCTCTT CATTGTCAGG ACACTGCTTC 
     1441 TGCACCCAGC TATTTTTGGC CTTCATCACA TTGGAATGCA GATGAGAATA GCCATGTTTA 
     1501 GCTTGATTTA TAAGAAGACT TTAAAGTTGT CAAGCCGTGT TCTTGATAAA ATAAGTATTG 
     1561 GACAACTTAT TAGTCTTCTT TCCAACAACC TGAACAAATT TGATGAAGGA CTTGCCTTGG 
     1621 CACATTTTAT ATGGATTGCT CCTTTACAAG TGGTTCTTCT GATGGGGCTT CTCTGGGACT 
     1681 TGTTACAGTT CTCAGCCTTC TGTGGCCTTG GTTTACTGAT AGTCTTGGTT ATTTTTCAAG 
     1741 CTATCTTAGG GAAGATGATG GTGAAGTACA GAGATAAAAG AGCTGCAAAG ATCAATGAAA 
     1801 GACTTGTGAT CACATCAGAA GTTATTGACA ATATCTATTC TGTTAAGGCA TATTGTTGGG 
     1861 AATCAGCGAT GGAGAAGATA ATTGAAAGCT TGAGAGAGGA GGAACTGAAA ATGACCCGGA 
     1921 GGTCAGCCTA TATGAGGTTC TTCACTAGCT CTGCCTTCTT CTTTTCAGGG TTTTTTGTAG 
     1981 TCTTTTTATC TGTGCTTCCC TACACAGTCA TCAATGGAAT CGTCCTCCGA AAAATATTCA 
     2041 CAACCATTTC ATTCTGCATT GTCCTGCGTA TGTCAGTCAC ACGGCAATTC CCCACTGCTG 
     2101 TACAGATATG GTATGATTCT TTGGGAATGA TAAGAAAAAT ACAGGATTTC CTACAGACTC 
     2161 AAGAATATAA AGTGCTGGAG TATAACTTAA TGTTCACAGG CCTAGTCATG GAGAATGTAA 
     2221 CAGCATTTTG GGAGGAGGGA TTTCAGGAAT TACTGGAGAA AGTACAACTA AACAATGATG 
     2281 ACAGAAAAAC TTCCAATGGT GAGAACCACC TCAGTTTTAG TCATCTCTGT CTAGTGGGAA 
     2341 ATCCTGTGTT GAAAAACATC AATTTGAATA TAAAGAAAGG AGAGATGTTG GCTATTACTG 
     2401 GATCTACTGG AGCAGGAAAG ACATCACTCC TGATGCTGAT ACTGGGAGAA CTGGAAGCGT 
     2461 CAGAGGGAAT TATTAAGCAC AGTGGAAGAG TTTCATTCTC CTCTCAAATT TCTTGGATTA 
     2521 TGCCGGGTAC TATCAAAGAA AATATCATCT TTGGTGTTTC CTATGATGAG TACAGATATA 
     2581 AGAGTGTTGT CAAAGCTTGC CAACTACAGG AGGACATCAC CAAGTTTGCA GAACAAGACA 
     2641 ACACAGTTCT TGGAGAAGGT GGAGTCACAC TCAGTGGAGG TCAACGTGCA AGAATTTCTT 
     2701 TAGCAAGAGC AGTATATAAA GATGCTGATT TGTACCTGTT AGATTCCCCC TTCGGATATC 
     2761 TAGATGTTTT AACTGAAGAA CAAATATTTG AAAGTTGTGT CTGTAAATTG ATGGCCAGCA 
     2821 AAACTAGGAT TCTGGTCACA TCTAAAATGG AGCAGTTAAA GAAAGCTGAC AAGATACTCA 
     2881 TTTTGCATGA GGGCAGCAGC TATTTTTATG GGACGTTTTC TGAACTACAA AGTCTACGTC 
     2941 CAGACTTCAG TTCCAAACTC ATGGGGTATG ATACTTTTGA CCAGTTTACT GAGGAAAGAA 
     3001 GGAGTTCAAT TCTAACTGAG ACCTTACGCA GGTTCTCAGT GGACGATGCC TCTACCACCT 
     3061 GGAACAAAGC CAAACAGTCA TTTAGACAGA CTGGAGAGTT TGGAGAAAAA AGGAAGAACT 
     3121 CTATTCTAAG TTCATTCAGC TCTGTGAAGA AAATTTCCAT TGTGCAAAAG ACTCCATTGT 
     3181 CCATAGAAGG AGAATCTGAT GACCTCCAAG AAAGGAGACT GTCCCTGGTT CCAGACTCTG 
     3241 AACATGGAGA GGCCGCTCTG CCTCGCAGCA ACATGATCAC CGCCGGGCCC ACGTTTCCAG 
     3301 GCAGAAGAAG ACAGTCTGTT TTGGATCTTA TGACATTCAC ACCCAGCTCA GTCTCCAGCA 
     3361 GTCTCCAGAG GACCAGAGCT TCTATTCGAA AAATATCCTT AGCCCCTCGG ATAAGCTTAA 
     3421 AGGAAGAGGA TATATATTCA AGGCGATTAT CGCAAGATAG CACACTGAAC ATCACCGAAG 
     3481 AAATTAATGA AGAAGATTTA AAGGAGTGTT TTTTTGATGA TATGGTAAAG ATCCCCACGG 
     3541 TGACAACATG GAACACATAC CTTCGATATT TCACGCTCCA CAGAGGCTTA TTTGCAGTGC 
     3601 TGATTTGGTG CGTGCTGGTT TTTCTGGTTG AGGTGGCTGC TTCTTTATTT GTGTTGTGGT 
     3661 TGCTTAAAAA CAACCCCGTT AATGGTGGAA ACAATGGCAC TAAAATTGCC AATACCTCCT 
     3721 ATGTTGTGGT CATCACCAGT TCCAGCTTCT ATTACATTTT TTACATTTAC GTGGGAGTGG 
     3781 CTGACACTTT GCTTGCTCTG AGCCTCTTCA GAGGTTTGCC CCTGGTGCAT ACGTTAATCA 
     3841 CAGCATCAAA AATTTTGCAC AGGAAAATGT TACATTCTAT TCTTCATGCC CCTATGTCAA 
     3901 CCTTCAACAA GCTGAAAGCA GGTGGGATTC TTAACAGATT CTCCAAAGAT ATAGCAATTT 
     3961 TGGATGACTT TCTGCCTCTT ACCATATTTG ACTTCATCCA GTTGCTGTTC ATTGTGGTCG 
     4021 GAGCAATAAT AGTTGTCTCG GCTTTACAAC CCTACATCTT CCTAGCAACG GTGCCAGGGC 
     4081 TAGCAGTCTT CATTTTACTG AGGGCATACT TCCTTCATAC CTCCCAACAG CTCAAACAAC 
     4141 TGGAATCTGA AGGCAGGAGT CCAATTTTCA CGCACCTTGT TACAAGCTTA AAAGGACTGT 
     4201 GGACACTTCG AGCCTTCCGA CGCCAGACTT ACTTTGAAAC CCTATTCCAC AAAGCTCTGA 
     4261 ATTTGCACAC TGCCAACTGG TTTATGTATC TGGCAACCTT GCGCTGGTTC CAGATGAGAA 
     4321 TAGACATGAT TTTCGTCCTC TTCTTCATTG TTGTTACCTT CATCTCCATT TTAACAACAG 
     4381 GTGAAGGAGA AGGAACGACT GGTATTATTC TAACTTTAGC TATGAATATT ATGAGTACTT 
     4441 TGCAATGGGC TGTGAACTCA AGCATTGATA CAGATAGCTT GATGCGATCT GTGAGCAGAG 
     4501 TCTTTAAGTT TATTGATATA CAAACAGAAG AAAGTATATG TACCAAGATA ATGAAAGAAC 
     4561 TACATAGTGA AGACTCACCT AATGCTTTAG TCATTAAGAA TGAACATGTG AAGAAATGTG 
     4621 ATACCTGGCC CTCTGGAGGT GAAATGGTTG TCAAAGACCT TACTGTGAAA TATGTGGATG 
     4681 ATGGGAATGC CATATTAGAG AACATTTCTT TTTCAATAAG TCCTGGCCAG AGGGTGGGGC 
     4741 TCTTAGGAAG AACTGGCTCA GGAAAAAGTA CTTTGCTGTC AGCCTTTTTA CGAATGTTGA 
     4801 ACATTAAAGG CGAAATACAG ATCGATGGTG TCTCCTGGAA CTCAATGACC TTACAAGAAT 
     4861 GGAGAAAAGC TTTTGGAGTG ATAACACAGA AAGTATTTAT CTTTTCTGGA ACATTCAGAC 
     4921 AAAACTTGGA TCCCAATGGA AAATGGAGAG ATGAAGAAAT ATGGAAAGTT GCAGATCAGG 
     4981 TTGGCCTCAA GTCTGTGATA GAGCAGTTTC CTGGACAGCT CAACTTTACC CTTGTGGATG 
     5041 GGGGTTATGT GCTAAGCCAT GGTCACAAGC AGTTGATGTG CTTGGCCCGA TCTGTTCTCA 
     5101 GTAAGGCCAA GATCATACTG CTTGATGAGC CTAGTGCCAA TCTAGACCCC ATAACATACC 
     5161 AAGTGATTCG ACGAGTTCTA AGACAAGCCT TCGCTGGTTG CACAGTAGTC CTCTGTGAAC 
     5221 ACAGGATAGA AGCAATGTTG GACTGTCAGC GATTTTTGGT CATAGAGCAG GGCAATGTCT 
     5281 GGCAGTATGA ATCCCTCCAG GCACTTCTGA GCGAGAAGAG TGTCTTCCAG CGAGCCCTTA 
     5341 GCTCCTCAGA AAAGATGAAG CTCTTCCACG GCCGGCACTC CAGCAAGCAG AAGCCTCGGA 
     5401 CACAGATCAC TGCTGTGAAA GAGGAGACGG AGGAAGAAGT GCAAGAGACC CGGCTCACGC 
     5461 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5521 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5581 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5641 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5701 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5761 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5821 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5881 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5941 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     6001 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     6061 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     6121 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     6181 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6241 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6301 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6361 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6421 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6481 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6541 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6601 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6661 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6721 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6781 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6841 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6901 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6961 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     7021 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     7081 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     7141 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     7201 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7261 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7321 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7381 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7441 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7501 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7561 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7621 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7681 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7741 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7801 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7861 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7921 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7981 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     8041 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     8101 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     8161 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     8221 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8281 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8341 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8401 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8461 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8521 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8581 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8641 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8701 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8761 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8821 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8881 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8941 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     9001 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     9061 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     9121 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     9181 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9241 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9301 GCGAATTTT
//