LOCUS RR209950 7149 bp DNA circular SYN DEFINITION Plcd1 (Myc-DDK-tagged ORF) - Rat phospholipase C, delta 1 (Plcd1), (10 ug) ACCESSION NM_017035 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3306..3397 /label="Myc-DDK" misc_feature 3427..4012 /label="PolyA signal" misc_feature 3484..3503 /label="Restriction Enzyme" misc_feature 4288..4960 /label="ColE1" misc_feature 5390..6184 /label="Kan/Neo" misc_feature 6219..6521 /label="SV40 ori" misc_feature 6607..7084 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3288..3294 /label="Restriction Enzyme" misc_feature 1020..3288 /label="Plcd1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGACTCGGGT AGGGACTTCC TGACCCTGCA CGGGCTCCAG GATGACCCGG 1081 ACCTTCAGGC CCTTCTGAAG GGCAGCCAGC TTCTGAAGGT GAAGTCCAGC TCGTGGCGTA 1141 GGGAACGCTT CTACAAGCTA CAGGAGGACT GCAAGACCAT CTGGCAGGAA TCTCGAAAGG 1201 TCATGAGGTC CCCGGAGTCG CAGCTGTTCT CCATCGAGGA CATTCAGGAG GTACGGATGG 1261 GACACCGCAC AGAAGGCCTG GAGAAGTTTG CCCGAGACAT CCCCGAGGAT CGATGCTTCT 1321 CCATTGTCTT CAAGGACCAG CGCAACACCC TAGACCTCAT TGCCCCATCA CCAGCTGACG 1381 CTCAGCACTG GGTGCAGGGC CTGCGCAAGA TCATCCACCA CTCCGGCTCC ATGGACCAGC 1441 GGCAGAAGCT GCAGCACTGG ATTCACTCCT GCTTGCGAAA GGCTGATAAA AACAAGGACA 1501 ACAAGATGAA CTTCAAGGAG CTGAAGGACT TCCTGAAGGA GCTCAACATC CAGGTGGATG 1561 ACGGCTACGC CAGGAAGATC TTCAGGGAAT GTGACCACTC CCAGACAGAC TCGCTGGAGG 1621 ATGAGGAGAT TGAGACCTTC TACAAGATGC TGACCCAGCG CGCAGAGATC GACCGTGCTT 1681 TTGAGGAGGC CGCAGGATCC GCGGAGACCC TGTCGGTGGA GAGGCTAGTG ACGTTTCTGC 1741 AGCACCAGCA GCGGGAGGAG GAGGCAGGGC CAGCCTTGGC CCTCTCTCTC ATTGAGCGCT 1801 ACGAGCCCAG TGAGACTGCC AAGGCCCAGC GGCAGATGAC CAAGGATGGC TTCCTCATGT 1861 ACTTGCTGTC GGCTGACGGT AACGCCTTCA GCCTGGCACA CCGCCGTGTC TACCAGGACA 1921 TGGACCAGCC ACTGAGTCAC TACTTAGTGT CTTCTTCCCA CAACACCTAC CTGCTGGAAG 1981 ACCAGCTCAC AGGGCCCAGC AGCACGGAGG CCTACATCCG GGCTCTGTGC AAAGGCTGCC 2041 GGTGCTTGGA GCTCGACTGC TGGGATGGTC CCAACCAGGA ACCCATCATC TACCACGGCT 2101 ACACTTTTAC CTCTAAGATA CTTTTCTGTG ACGTGCTCAG GGCCATCAGG GACTATGCCT 2161 TCAAGGCATC CCCCTACCCA GTCATCCTGT CCCTGGAGAA CCACTGTAGC CTGGAGCAAC 2221 AACGAGTCAT GGCCCGTCAC CTGAGGGCTA TCCTGGGGCC CATACTGTTG GACCAGCCAC 2281 TGGATGGGGT TACCACGAGC CTGCCTTCAC CTGAGCAACT GAAGGGCAAG ATCCTGTTGA 2341 AAGGGAAGAA GCTGGGAGGG CTGCTGCCTG CTGGAGGGGA GAATGGGTCC GAAGCCACTG 2401 ACGTGTCTGA CGAGGTAGAG GCTGCTGAAA TGGAGGACGA GGCTGTGCGC AGCCAAGTGC 2461 AACACAAGCC CAAGGAGGAT AAACTAAAGC TGGTGCCGGA GCTCTCCGAC ATGATCATTT 2521 ACTGCAAGAG CGTCCACTTT GGTGGCTTCT CCAGTCCTGG CACCTCTGGG CAGGCATTCT 2581 ATGAGATGGC TTCCTTCTCT GAGAGCCGTG CCCTCCGGTT GCTGCAAGAA TCAGGAAATG 2641 GTTTTGTCCG TCATAACGTG AGCTGTCTGA GCAGGATCTA CCCGGCTGGG TGGAGAACAG 2701 ATTCCTCCAA CTACAGTCCT GTGGAGATGT GGAACGGGGG CTGCCAGATT GTTGCTCTGA 2761 ACTTCCAAAC CCCTGGGCCA GAAATGGATG TTTACCTTGG CTGCTTCCAG GACAATGGAG 2821 GCTGTGGGTA TGTGCTGAAA CCCGCCTTTC TGAGAGACCC CAACACCACC TTCAACTCAC 2881 GTGCCCTGAC TCAGGGGCCC TGGTGGCGTC CAGAGAGGCT CCGTGTCCGG ATCATCTCTG 2941 GACAGCAGCT GCCAAAGGTC AATAAGAATA AGAATTCTAT CGTGGACCCC AAGGTGATCG 3001 TGGAGATCCA TGGCGTGGGC CGGGACACCG GTAGCCGTCA GACAGCAGTT ATTACCAATA 3061 ATGGTTTCAA CCCACGGTGG GACATGGAGT TTGAGTTTGA GGTAACAGTG CCTGACCTTG 3121 CCCTCGTACG CTTCATGGTA GAGGATTATG ATTCCTCTTC TAAGAACGAC TTTATTGGCC 3181 AGAGTACTAT CCCCTGGAAC AGCCTCAAGC AGGGGTACCG CCATGTCCAC CTCTTGTCTA 3241 AGAATGGAGA CCAGCATCCA TCAGCCACAC TTTTTGTGAA GATCTCCATC CAGGACACGC 3301 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 3361 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 3421 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 3481 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 3541 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 3601 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 3661 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 3721 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 3781 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 3841 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 3901 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 3961 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 4021 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 4081 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 4141 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 4201 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 4261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 4321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 4381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 4441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 4501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 4561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 4621 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 4681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 4741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 4801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 4861 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 4921 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 4981 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 5041 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 5101 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 5161 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 5221 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 5281 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 5341 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 5401 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 5461 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 5521 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 5581 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 5641 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 5701 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 5761 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 5821 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 5881 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 5941 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 6001 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 6061 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 6121 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 6181 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 6241 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 6301 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 6361 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 6421 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 6481 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 6541 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 6601 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 6661 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 6721 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 6781 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 6841 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 6901 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 6961 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 7021 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 7081 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 7141 GCGAATTTT //