LOCUS       RR209940                8481 bp    DNA     circular  SYN 
DEFINITION  Taf2 (Myc-DDK-tagged ORF) - Rat TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf2), (10 ug)
ACCESSION   NM_133319
VERSION     NM_133319.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4638..4729 
                     /label="Myc-DDK"  
     misc_feature    4759..5344 
                     /label="PolyA signal"  
     misc_feature    4816..4835 
                     /label="Restriction Enzyme"  
     misc_feature    5620..6292 
                     /label="ColE1"  
     misc_feature    6722..7516 
                     /label="Kan/Neo"  
     misc_feature    7551..7853 
                     /label="SV40 ori"  
     misc_feature    7939..8416 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4620..4626 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4620 
                     /label="Taf2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGCCCACC GGCTTAGAGT CCTCCAGGAT GAACAGGAAG AAAGGAGACA 
     1081 AGGGCTTCGA AAGCCCACGG CCGTACAAAT TAACCCATCA GGTCGTCTGC ATCAACAACA 
     1141 TAAACTTCCA GAGAAAGTCT GTTGTGGGGT TTGTGGAGCT GACTATATTT CCTACGGTTG 
     1201 CAAACTTGAA TAGAATCAAG CTGAACAGTA AACAGTGTAG AATATACCGA GTCAGGATCA 
     1261 ATGACTTGGA GGCTGCTTTT ATTTATAATG ATCCAACCTT AGAAGTTTGT CACAGCGAAT 
     1321 CAAAACAGAG AAACCTCAAT TATTTTTCCA ATGCGTATGC AGCTGCAGTC AGTGCTGTGG 
     1381 ACCCTGATGC GGGGAACGGA GAACTCTGCA TTAAGGTCCC CTCAGAGCTC TGGAAGCACG 
     1441 TCGATGAGTT AAAGGTCCTG AAGATACATA TTAATTTTTC TTTGGATCAG CCAAAAGGAG 
     1501 GTCTTCATTT TGTGGTACCC AGTGTAGAGG GGAGTATGGC AGAGAGAGGT GCTCATGTCT 
     1561 TCTCTTGTGG GTATCAGAAT TCTACAAGAT TTTGGTTCCC TTGTGTTGAT TCATACTCTG 
     1621 AGTTGTGTAC CTGGAAATTA GAATTTACAG TCGATGCTGC GATGGTGGCT GTGTCCAATG 
     1681 GAGATTTGGT GGAGACAGTG TATACTCACG ACATGAGAAA GAAGACCTTC CATTATGTGC 
     1741 TTGCTATTCC TACAGCAGCG TCCAATATCT CCTTGGCCAT CGGGCCATTT GAAATACTTG 
     1801 TAGATCCATA TATGCACGAG GTTACCCATT TTTGTTTGCC TCAACTTCTT CCATTACTTA 
     1861 AACATACTAC ATCATACCTT CACGAAGTTT TTGAATTTTA TGAGGAAATT TTGACTTGTC 
     1921 GCTATCCGTA CTCCTGTTTT AAGACGGTCT TCATTGATGA GGCTTACGTG GAAGTGGCCG 
     1981 CTTATGCGTC TATGAGCATT TTCAGCACAA ATCTCCTGCA CAGCGCCATG ATTATAGATG 
     2041 AGACACCGCT CACCAGGCGG TGCCTCGCAC AGTCTCTAGC ACAGCAGTTC TTCGGATGTT 
     2101 TCATCTCCAG AATGTCTTGG TCTGATGAGT GGGTGCTGAA GGGAATTTCA GGTTATATTT 
     2161 ATGGACTGTG GATGAAGAAA ACGTTTGGTG TCAATGAGTA CCACCATTGG ATTAAAGAGG 
     2221 AACTCGATAA AATAGTGGCA TATGAACTGA AAACTGGGGG AGTTTTACTG CATCCCATAT 
     2281 TTGGTGGTGG AAAAGAAAAG GATAACCCCG CATCTCACCT CCACTTTTCA ATAAAGCATC 
     2341 CACATACACT GTCTTGGGAA TACTACACTA TGTTTCAGTG TAAAGCTCAC CTCGTGATGA 
     2401 GACTGATTGA AAACAGAATC AGCATGGAAT TTATGCTGCA AGTTTTCAAT AAACTATTAA 
     2461 GTCTGGCCAG TACCGCTTCA TCCCAGAAGT TCCAGTCACA TATGTGGAGC CAGATGCTGG 
     2521 TCTCCACCTA CGGGTTTTTG AAGTCCATTT CCAATGTCTC TGGCAAAGAC ATCCAGCCCT 
     2581 TAATAAAGCA GTGGGTAGAC CAGAGTGGAG TGGTAAAGTT TTATGGAAGT TTTGCATTTA 
     2641 ATAGAAAACG AAATGTTTTA GAACTAGAAA TAAAACAAGA TTACACATCT CCTGGCACCC 
     2701 AGAAGTACGT GGGACCACTT AAAGTGACAG TGCAAGAGTT AGACGGCTCC TTCAACCATA 
     2761 CCTTGCAAAT TGAAGAAAAC AGCCTTAAAC ATGACATACC CTGCCACTCC AAAAGCAGAA 
     2821 GGAATAAGAA GAAGAAAATC CCACTGATGA ATGGGGAAGA AGTCGATATG GATCTTTCTG 
     2881 CAATGGATGC GGACTCCCCT TTGCTGTGGA TAAGGATAGA CCCAGATATG TCTGTCCTGA 
     2941 GGAAGGTGGA GTTTGAGCAG GCTGACTTTA TGTGGCAGTA TGAGCTCCGC TATGAGAGAG 
     3001 ATGTTGTTGC TCAGCAGGAA TCCATCTTGG CCTTGGAAAA GTTCCCCACG CCCGCATCGC 
     3061 GCCTCGCGCT CACTGATATA CTAGAGCAAG AGCAGTGTTT CTACCGAGTG AGGATGTCAG 
     3121 CATGCTTCTG CCTTGCAAAG ATTGCAAACT CAATGGTGAG CACATGGACG GGACCACCAG 
     3181 CCATGAAGTC CCTCTTTACT AGAATGTTTT GTTGTAAAAC TTGTCCCAAC ATCGTGAAAA 
     3241 CAAACAACTT CATGAGCTTT CAGAGTTATT TTCTACAGAA GACTATGCCA GTTGCAATGG 
     3301 CTTTGTTAAG AGACGTACAT AATCTTTGTC CCAAAGAAGT TTTAACATTT ATTTTAGACT 
     3361 TAATCAAGTA CAATGACAAC AGAAAAAATA AGTTTTCAGA TAACTATTAC CGTGCAGAAA 
     3421 TGATTGATGC CTTGGCTAAC TCTGTCACAC CTGCTGTCAG CGTGAATAAT GAAGTCAGAA 
     3481 CTTTGGATAA CTTAAATCCT GATGTGCGAC TAATACTTGA AGAAATTACC CGGTTTTTGA 
     3541 ATATGGAAAA ACTTCTCCCA AGTTACAGAC ATACTATCAC TGTCAGTTGT TTGAGAGCCA 
     3601 TAAGAGTGCT TCAGAAGAAT GGACATGTGC CAAGTGATGC ATCTCTTTTC AAATCCTATG 
     3661 CTGAGTATGG CCACTTCGTG GACATCAGGA TAGCGGCTCT GGAAGCCGTC GTCGATTACA 
     3721 CCAAAGTGGA CAGGAGTTAT GAAGAACTTC AATGGCTACT TAATATGATT CAGACTGACC 
     3781 CTGTCCCCTA TGTGAGGCAT AAGATTCTAA ACATGCTGAC TAAAAATCCG CCATTTACAA 
     3841 AGAACATGGA GTCTCCTTTA TGCAACGAGG CCTTGGTAGA TCAGCTCTGG AAGCTGATGA 
     3901 ATTCTGGTAC TGCACATGAC TGGAGGTTAC GGTGTGGAGC TGTGGACTTG TACTTCACGC 
     3961 TCTTTGGCCT CAGTAGACCT TCTTGTTTAC CCTTGCCAGA GCTCGGGCTT GTTCTTAACC 
     4021 TAAAAGAGAA GAAAGCTGTC TTGAATCCAA CCATAATTCC AGAAGCGGGA GTAGGCAATC 
     4081 AGGAAACTGC AAGTAACCCA GGCTGTCACA CTCAGCTAGC AGGATTTCAG AACCCTTTCT 
     4141 CAAGTTCTCA GGATGAGGAG GAGGTCGATA TGGACACCGT TCATGATAGT CAGGCCTTCA 
     4201 TTTCCCATCA TCTGAACATG CTTGAAAGGC CGTCCACTCC AGGGCTCTCT AAGTACCGAC 
     4261 CATCCAGCTC CAGGTCTTCC TTAATGCCCC AGCATGCATT AGGCTGTGAC ATCACACCAA 
     4321 CCACAAAACC CCAGTGGAGT ATGGAACTGT CCCGAAAGGG AGCAGGTAAA GAGCAGCCTT 
     4381 TGGAGATGGG TGTGCATCCC GTGGCGGCCC CGCTCTCCGT GTTTGCTAAG GAGGCTCTGT 
     4441 CCTCTGCCCG GCACAGCGAG CACCACCACC ACCATCACCA TGAGCACAAG AAGAAGAAGA 
     4501 AGAAGCACAA ACACAAGCAC AAGCACAAGC ACAAGCATGA CGGCAAGGAC AAGGACAAGG 
     4561 ACAAGGAGCC CTTCACCTTC TCCAGCCCTG CCAGTGGCAG GTCTGTGCGC TCCCCTTCAC 
     4621 TCTCAGACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4681 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4741 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4801 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4861 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4921 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4981 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5041 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5101 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5161 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5221 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5281 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5341 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5401 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5461 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5521 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5581 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5641 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5701 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5761 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5821 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5881 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5941 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6001 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6061 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6121 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6181 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6241 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6301 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6361 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6421 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6481 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6541 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6601 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6661 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6721 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6781 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6841 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6901 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6961 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7021 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7081 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7141 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7201 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7261 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7321 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7381 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7441 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7501 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7561 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7621 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7681 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7921 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7981 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8041 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8101 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8161 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8221 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8281 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8341 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8401 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8461 CAAAAATTTA ACGCGAATTT T
//