LOCUS       RR209809                8370 bp    DNA     circular  SYN 
DEFINITION  Kcnh2 (Myc-DDK-tagged ORF) - Rat potassium voltage-gated channel, subfamily H (eag-related), member 2 (Kcnh2), (10 ug)
ACCESSION   NM_053949
VERSION     NM_053949.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4527..4618 
                     /label="Myc-DDK"  
     misc_feature    4648..5233 
                     /label="PolyA signal"  
     misc_feature    4705..4724 
                     /label="Restriction Enzyme"  
     misc_feature    5509..6181 
                     /label="ColE1"  
     misc_feature    6611..7405 
                     /label="Kan/Neo"  
     misc_feature    7440..7742 
                     /label="SV40 ori"  
     misc_feature    7828..8305 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4509..4515 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4509 
                     /label="Kcnh2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGTGCGG AGGGGCCACG TCGCGCCGCA GAACACCTTC CTCGACACCA 
     1081 TCATCCGCAA GTTTGAGGGC CAGAGCCGCA AGTTCATCAT CGCTAACGCT CGTGTAGAGA 
     1141 ACTGCGCCGT CATCTACTGC AACGACGGCT TCTGCGAACT GTGCGGCTAC TCTCGGGCCG 
     1201 AGGTGATGCA GCGGCCCTGC ACCTGCGATT TCCTGCATGG GCCGCGCACG CAGCGCCGTG 
     1261 CCGCCGCGCA GATCGCGCAG GCCCTGCTGG GCGCGGAGGA GCGCAAAGTG GAGATCGCCT 
     1321 TCTACCGGAA AGATGGGAGC TGCTTCCTGT GTTTGGTGGA TGTGGTGCCC GTGAAGAATG 
     1381 AGGATGGGGC TGTCATCATG TTTATCCTCA ACTTTGAAGT GGTGATGGAG AAGGACATGG 
     1441 TAGGGTCGCC AGCTCATGAC ACCAATCACA GGGGGCCCTC TACCAGCTGG CTAGCTTCTG 
     1501 GCCGGGCCAA GACCTTCCGC CTGAAGCTGC CTGCCTTGTT GGCACTGACT GCCAGGGAAT 
     1561 CGCCCATGCG GACAGGAAGC ACGGGCAGTC CTGGAGCCCC CGGGGCAGTG GTGGTGGATG 
     1621 TGGACCTGAC GCCGGCAGCA CCCAGCAGTG AGTCCCTGGC CTTGGATGAG GTTTCTGCCA 
     1681 TGGACAACCA TGTGGCTGGA CTTGGGCCTG CAGAAGAGAG GCGAGCACTG GTGGGCCCAG 
     1741 CGTCTGCTTC ACCGGTAGCC AGCATCCCAG GCCCTCACCC GTCCCCTCGA GCTCAGAGCC 
     1801 TTAACCCTGA CGCCTCTGGT TCCAGCTGCA GTCTGGCCCG AACACGCTCA CGGGAGAGCT 
     1861 GCGCTAGTGT GCGCCGGGCG TCTTCCGCAG ATGACATCGA GGCAATGCGG GCTGGGGCGC 
     1921 TGCCCCTCCC GCCCCGACAT GCTAGTACAG GGGCCATGCA CCCCCTGCGC AGTGGCCTGC 
     1981 TTAACTCCAC CTCAGACTCT GACCTCGTGC GCTACCGAAC CATTAGCAAG ATACCCCAAA 
     2041 TCACCCTCAA CTTTGTGGAC CTCAAAGGCG ACCCTTTCCT GGCTTCACCC ACCAGTGACC 
     2101 GGGAAATCAT AGCACCCAAG ATAAAAGAGC GGACCCACAA TGTCACCGAG AAGGTCACCC 
     2161 AGGTCCTGTC CTTGGGAGCC GATGTGCTTC CTGAATATAA GCTGCAGGCC CCAAGAATAC 
     2221 ACCGCTGGAC CATCCTACAC TACAGCCCCT TCAAGGCAGT GTGGGACTGG CTCATTCTGC 
     2281 TGCTGGTCAT CTACACAGCC GTCTTCACAC CGTACTCGGC TGCCTTCCTG CTGAAGGAGA 
     2341 CTGAAGATGG GTCCCAAGCC CCTGACTGCG GCTATGCCTG CCAGCCTCTG GCTGTAGTGG 
     2401 ACCTCCTCGT GGACATCATG TTCATTGTGG ACATCCTTAT CAATTTCCGC ACCACCTACG 
     2461 TTAACGCCAA TGAAGAGGTG GTCAGCCATC CTGGCCGCAT CGCTGTGCAC TACTTCAAGG 
     2521 GCTGGTTCCT CATCGACATG GTGGCTGCCA TCCCCTTCGA CCTGCTCATC TTTGGCTCTG 
     2581 GCTCTGAGGA GCTCATCGGA CTGCTGAAAA CTGCCAGGCT GCTGCGGCTG GTGCGAGTGG 
     2641 CTCGGAAGCT GGACCGCTAC TCGGAGTATG GGGCAGCAGT GCTCTTCCTG CTCATGTGCA 
     2701 CCTTCGCGCT CATTGCACAC TGGTTGGCCT GCATCTGGTA CGCCATCGGC AACATGGAAC 
     2761 AGCCTCACAT GGACTCACAC ATTGGCTGGC TGCACAACTT GGGCGACCAG ATCGGCAAGC 
     2821 CCTACAACAG CAGCGGCCTG GGAGGCCCCT CCATCAAGGA CAAGTACGTC ACAGCGCTCT 
     2881 ACTTCACCTT CAGCAGCCTC ACCAGCGTGG GCTTTGGCAA TGTCTCTCCC AACACCAACT 
     2941 CAGAAAAGAT CTTCTCCATC TGTGTCATGC TCATTGGCTC CCTCATGTAT GCCAGCATCT 
     3001 TCGGCAACGT GTCCGCCATC ATCCAGCGGC TGTACTCGGG CACGGCCCGC TACCACACAC 
     3061 AGATGCTCCG GGTGCGGGAG TTTATCCGCT TCCATCAGAT TCCCAACCCG CTACGCCAGC 
     3121 GCCTCGAGGA GTACTTCCAG CACGCTTGGT CCTACACCAA CGGCATCGAC ATGAATGCGG 
     3181 TGCTGAAGGG TTTCCCCGAG TGCCTGCAGG CTGACATCTG CCTACATCTA AACCGCTCAC 
     3241 TGCTGCAGCA CTGCAAACCA TTCCGAGGGG CCACTAAAGG CTGCCTGAGG GCCCTGGCCA 
     3301 TGAAATTCAA GACCACACAT GCGCCACCAG GGGACACGCT GGTGCACGCT GGGGACCTGC 
     3361 TTACTGCCCT CTACTTCATC TCCAGGGGCT CCATCGAGAT CCTGCGGGGT GATGTCGTGG 
     3421 TGGCCATCTT GGGGAAGAAC GACATCTTTG GAGAGCCTCT GAACCTGTAT GCACGTCCTG 
     3481 GGAAGTCCAA TGGGGATGTG AGGGCCCTCA CTTACTGTGA CCTGCACAAG ATCCATCGAG 
     3541 ACGATCTGCT GGAGGTGTTG GACATGTACC CCGAGTTCTC AGACCACTTT TGGTCTAGCC 
     3601 TGGAGATCAC CTTCAACCTT CGAGATACCA ACATGATTCC TGGCTCCCCC AGCAGTGCGG 
     3661 AACTAGAGAG CGGCTTCAAC AGGCAGCGAA AGCGCAAACT GTCCTTCCGC AGGCGTACTG 
     3721 ACAAGGACAC AGAGCAGCCA GGGGAGGTGT CAGCCCTGGG GCAAGGCCCT GCCCGAGTTG 
     3781 GGCCAGGGCC GAGTTGCCGG GGACAGCCGG GAGGGCCATG GGGGGAGAGC CCATCCAGTG 
     3841 GCCCTTCCAG CCCAGAGAGC AGTGAGGATG AGGGCCCAGG TCGCAGCTCC AGCCCCCTCC 
     3901 GCCTGGTGCC CTTCTCCAGC CCCAGGCCTC CCGGAGACTC CCCAGGTGGG GAGCCTTTGA 
     3961 CAGAGGATGG AGAGAAAAGC AGTGACACTT GTAATCCCCT GTCAGGTGCC TTCTCTGGGG 
     4021 TGTCCAATAT TTTCAGCTTC TGGGGGGACA GTCGGGGGCG CCAGTACCAG GAGCTGCCTC 
     4081 GATGCCCTGC CCCGGCCCCT AGCCTCCTCA ACATCCCCTT GTCTAGCCCT GGCCGGCGTT 
     4141 CCCGGGGTGA CGTGGAGAGC AGGCTGGATG CACTCCAGAG ACAGCTGAAC AGGCTGGAAA 
     4201 CCCGGCTAAG TGCAGACATG GCCACTGTCC TACAGCTGCT ACAGAGGCAA ATGACCCTGG 
     4261 TCCCTCCTGC CTACAGTGCT GTGACCACCC CTGGGCCTGG CCCCACTTCC ACGTCCCCTT 
     4321 TGTTGCCTGT CGGCCCCGTC CCCACTCTCA CCCTGGACTC GCTTTCTCAG GTTTCCCAGT 
     4381 TCGTGGCGTT TGAGGAGCTT CCTGCAGGAG CCCCAGAGCT CCCCCAAGAT GGCCCCACTC 
     4441 GACGCCTCTC CCTGCCGGGC CAGCTGGGGG CCCTCACCTC CCAGCCCCTG CACAGACATG 
     4501 GCTCAGATCC AGGCAGTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4561 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4621 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4681 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4741 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4801 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4861 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4921 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4981 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5041 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5101 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5161 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5221 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5281 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5341 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5401 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5461 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5521 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5581 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5641 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5701 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5761 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5821 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5881 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5941 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6001 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6061 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6121 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6181 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6241 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6301 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6361 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6421 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6481 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6541 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6601 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6661 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6721 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6781 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6841 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6901 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6961 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7021 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7081 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7141 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7201 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7261 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7321 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7381 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7441 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7501 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7561 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7621 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7681 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7741 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7801 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7861 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7921 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7981 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8041 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8101 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8161 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8221 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8281 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8341 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//