LOCUS       RR209788                7350 bp    DNA     circular  SYN 
DEFINITION  Pde8a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 8A (Pde8a), (10 ug)
ACCESSION   NM_198767
VERSION     NM_198767.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3507..3598 
                     /label="Myc-DDK"  
     misc_feature    3628..4213 
                     /label="PolyA signal"  
     misc_feature    3685..3704 
                     /label="Restriction Enzyme"  
     misc_feature    4489..5161 
                     /label="ColE1"  
     misc_feature    5591..6385 
                     /label="Kan/Neo"  
     misc_feature    6420..6722 
                     /label="SV40 ori"  
     misc_feature    6808..7285 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3489..3495 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3489 
                     /label="Pde8a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTGCGCC CCGAGCATCC ACATTTCCGA CAACCGAGTG TCGAACCCCA 
     1081 GCGACAGCGA CGGCGAGGAC GAGGACGTCC GGGTCCCCGC GCTGAGGTCT AGACAGCGAT 
     1141 GGTCGACCCC GCCCGGGCTC GTGGAGCCTC AGCCTCGCGA CAGCGGTGCC TCGAAGGTAT 
     1201 CAGTTGCTGA TGTGCAATTT GGCCCGATGA GATTTCATCA AGACCAGCTT CAGGTACTTT 
     1261 TAGTGTTTAC CAAAGAAGAT AGCCAGTGTA ATGGATTCTA CAGGGCATGT GAAAAAGCCG 
     1321 GGTTTAAGTG CACAGTCACC AAGGAGGTGC AGACTGTCCT TGCCTGTTTC CAGGACAAAC 
     1381 TCCATGACAT CATCATCATA GACCACAGAA ATCCCCGACA GATGGATGCG GAGGCAATGT 
     1441 GCAGGTCTAT CAGATCATCA AAATTATCAG AAAACACAGT TATTGTTGGC GTAGTACGCA 
     1501 GGGTGGACAA AGAAGAGGTG TCTTTAATGC CCTTCCTTGC CGCTGGCTTC ACGCGGAGGT 
     1561 ATATAGAAAA CCCCAACGTC ATGGCCTGTT ATAATGAACT GCTCCAGTTG GCATTTGGAG 
     1621 AGGTGCGATC ACAGCTGAAA CTCAGGGCTT GTAACTCAGT GTTCACTGCG TTAGAGAAGA 
     1681 GTCAAGAAGC GATTGAGATC ACAAGTGAGG ACCACATCAT CCAGTATGCA AACCCTGCAT 
     1741 TTGAAACAAC AATGGGTTAT CAGTCGGGAG AACTAATAGG GAAGGAGTTA GCACAAGTGC 
     1801 CCATCAATGA GAAGAAAGGT GACTTGCTGG ATGCCATAAA TTCCTGTGTC ACAATCGACA 
     1861 AGGAGTGGCA AGGAGTTTAC CATACCCAAA AGAAAAATGG AGATAACATC CAACAAAATG 
     1921 TGAAGATAAT ACCTGTCATT GGGCAGGGAG GAAAAATCAG ACACTATGTG TCCATTATCA 
     1981 GAGTGTGCAA TGGCAGCAAT AAGGTTGAGA CCACAACTGA GTGTGTTCAG ACTGATAGTC 
     2041 AAACAGATAG TCAGGCAGGC AAGCATAAAG ACAGGAGGAA ACATTCGATG GACGTCAAAG 
     2101 CGGTTTCCTC CAGAACAAGC GAAGTTTCCA ATCAGAGAAG ACATTCCTCT CTGGCCCGGA 
     2161 TACATTCTAT GATGATTGAG GCACCAATCA CCAAGGTAAT CAATATTCTT AGTGCTGCCC 
     2221 AGGAAAACAG TCCTGTGTCT GTGACAGAAG CCTTGAACCA TGTGCTGGAT ATCCTGAGAA 
     2281 CCACTGAACT GTATTCACCC CAGTTTAATA CTGAGGATGA CCCTCACGCC ACTGACCTCG 
     2341 TTGGGGGCTT AATGTCTGAT GGCTTACGGA GGTTTTCAGG AAATGAGTAT ACTCTTGCAA 
     2401 CAAAAAGCCT TCGGCCATCT TCAAGCCAGT TGGCCGTCCC CATCTCCCTT CATGATATCC 
     2461 CACCACGGAT TGCTCAGGTC ATAGAAAATG AGGAGCAATG GGACTTTGAC ATTTTTGAGC 
     2521 TGGAAGCTGC AACCCAAAAT AGGCCTTTGA TTTATCTAGG TCTCAAAACG TTTGCTCGTT 
     2581 TTGAAATCTG TGAGTTCTTA CAGTGCTCTG AGGCAACGCT GAAATCATGG TTTCAAATTA 
     2641 TTGAATCCAA TTACCATTCC TCTAACCCCT ACCACAATTC CACGCACGCC GCGGATGTGC 
     2701 TTCACGCCAC TGCGTATTTC CTTTCCAGAA ACAAGTTAAA GGACACTTTA GATCGCCTTG 
     2761 ATGAGGTCGC TGCCCTCATC GCTGCCACCA TCCACGATGT GGATCACCCC GGGAGAACCA 
     2821 ACTCTTTCCT CTGCAATGCG GGAAAGGAGC TGGCCGTACT GTACAATGAC ACTGCTGTGC 
     2881 TAGAGAGCCA CCACGCAGCG CTGGCCTTCC AGCTGACCCT TGAAAATGAC AAATGCAATA 
     2941 TCTTTAAAAA CACAGACAGG AATGACTACC GGACACTACG CCAGAGTATC ATTGACATGG 
     3001 TCCTAGCCAC AGAGATGACA AAGCACTTTG AACACGTCAA CAAATTTATC AACAGCATCA 
     3061 ACAAGCCTCT GAAAGCACAG AAAAACGAGG TTCAGGAAAC TGGCAGAAGT CTGGAAGATA 
     3121 TAAGAGCCAT GGTCAGGACT CCAGAGAGCC GGACTCTCAT CAAAAGGATG ATGATTAAGT 
     3181 GTGCCGACGT GTCCAACCCC TGCCGGCCCC TCGAGCACTG CATCGAGTGG GCTGCACGCA 
     3241 TCTCAGAGGA GTATTTCTCC CAGACCGATG AAGAGAAACA GCTGGACTTG CCTGTGGTGA 
     3301 TGCCAGTGTT TGACAGAAAC ACCTGCAGCA TCCCCAAGTC TCAGATCTCC TTCATCGACT 
     3361 ACTTCATCAC AGACATGTTT GATGCCTGGG ATGCCTTTGT TTACCTGCCT AACCTTATGC 
     3421 GGCACCTGGA GGACAACTTC AAGTACTGGA AAGGACTGGA TGAGAAGGAG CTGTGCACCC 
     3481 TCCGGCCCCC TCCAGAAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3541 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3601 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3661 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3721 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3781 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3841 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3901 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3961 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4021 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4081 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4141 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4201 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4261 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4321 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4381 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4441 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4501 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4561 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4621 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4681 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4741 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4801 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4861 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4921 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4981 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5041 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5101 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5161 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5221 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5281 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5341 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5401 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5461 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5521 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5581 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5641 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5701 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5761 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5821 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5881 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5941 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6001 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6061 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6121 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6181 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6241 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6301 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6361 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6421 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6481 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6541 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6601 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6661 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6721 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6781 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6841 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6901 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6961 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7021 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7081 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7141 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7201 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7261 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7321 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//